Restriction map for
pF1KB4058
(Circular) (Six-base) MAPSORT of: pF1KB4058.seq Check: 2098 from: 1 to: 4724
pF1KB4058 4724 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1762 1762
Size: 4724
Acc65I G'GTAC_C
Cuts at: 1740 1740
Size: 4724
AclI AA'CG_TT
Cuts at: 4564 4564
Size: 4724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 2549 2981 3334 3996 656
Size: 1893 432 353 662 1384
Fragments arranged by size:
1893 1384 662 432 353
AflII C'TTAA_G
Cuts at: 1621 1621
Size: 4724
AflIII A'CryG_T
Cuts at: 2076 3876 4157 2076
Size: 1800 281 2643
Fragments arranged by size:
2643 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 2029 3467 2029
Size: 1438 3286
ApaI G_GGCC'C
Cuts at: 462 462
Size: 4724
ApaLI G'TGCA_C
Cuts at: 1315 3562 1315
Size: 2247 2477
ApoI r'AATT_y
Cuts at: 1728 4077 4333 1728
Size: 2349 256 2119
Fragments arranged by size:
2349 2119 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
AvaI C'yCGr_G
Cuts at: 994 1744 994
Size: 750 3974
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1537 1570 1537
Size: 33 4691
BamHI G'GATC_C
Cuts at: 237 1016 1175 1749 237
Size: 779 159 574 3212
Fragments arranged by size:
3212 779 574 159
BanI G'GyrC_C
Cuts at: 1740 2154 2402 2437 1740
Size: 414 248 35 4027
Fragments arranged by size:
4027 414 248 35
BanII G_rGCy'C
Cuts at: 462 499 993 1738 2768 462
Size: 37 494 745 1030 2418
Fragments arranged by size:
2418 1030 745 494 37
BbeI G_GCGC'C
Cuts at: 2158 2406 2158
Size: 248 4476
BbsI GAAGACnn'nnnn_
Cuts at: 1300 1588 1300
Size: 288 4436
BbvCI CC'TCA_GC
Cuts at: 140 140
Size: 4724
BciVI GTATCCnnnnn_n'
Cuts at: 2622 3678 4724 2622
Size: 1056 1046 2622
Fragments arranged by size:
2622 1056 1046
BclI T'GATC_A
Cuts at: 1206 2244 1206
Size: 1038 3686
BfrBI ATG'CAT
Cuts at: 192 1525 192
Size: 1333 3391
BglII A'GATC_T
Cuts at: 2239 4276 2239
Size: 2037 2687
BlpI GC'TnA_GC
Cuts at: 1836 1836
Size: 4724
Bme1580I G_kGCm'C
Cuts at: 462 1319 2349 2442 3566 462
Size: 857 1030 93 1124 1620
Fragments arranged by size:
1620 1124 1030 857 93
BmrI ACTGGGnnnn_n'
Cuts at: 502 1936 2017 2352 4496 502
Size: 1434 81 335 2144 730
Fragments arranged by size:
2144 1434 730 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 4724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 674 1042 1356 1552 533
Size: 141 368 314 196 3705
Fragments arranged by size:
3705 368 314 196 141
Bpu10I CC'TnA_GC
Cuts at: 140 744 1559 140
Size: 604 815 3305
Fragments arranged by size:
3305 815 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 1900 2779 3493 3791 1717
Size: 183 879 714 298 2650
Fragments arranged by size:
2650 879 714 298 183
BsaI GGTCTCn'nnnn_
Cuts at: 1528 4428 1528
Size: 2900 1824
BsaAI yAC'GTr
Cuts at: 1314 2077 1314
Size: 763 3961
BsaBI GATnn'nnATC
Cuts at: 6 2238 4275 6
Size: 2232 2037 455
Fragments arranged by size:
2232 2037 455
BsaHI Gr'CG_yC
Cuts at: 2155 2403 3105 4601 2155
Size: 248 702 1496 2278
Fragments arranged by size:
2278 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1607 1913 2434 3112 3523 3670 3944 1607
Size: 306 521 678 411 147 274 2387
Fragments arranged by size:
2387 678 521 411 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 1174 1204 1504 1534 349
Size: 30 795 30 300 30 3539
Fragments arranged by size:
3539 795 300 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 354 395 952 1012 1234 354
Size: 41 557 60 222 3844
Fragments arranged by size:
3844 557 222 60 41
BseYI C'CCAG_C
Cuts at: 982 2149 2907 3572 3980 982
Size: 1167 758 665 408 1726
Fragments arranged by size:
1726 1167 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 831 658
Size: 173 4551
BsiEI CG_ry'CG
Cuts at: 104 2266 2312 3542 104
Size: 2162 46 1230 1286
Fragments arranged by size:
2162 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1319 1738 2516 2706 3566 1319
Size: 419 778 190 860 2477
Fragments arranged by size:
2477 860 778 419 190
Bsp1286I G_dGCh'C
Cuts at: 462 499 993 1319 1738 2349 2442 2516 2706
Size: 37 494 326 419 611 93 74 190
Cuts at: 2706 2768 3566 462
Size: 62 798 1620
Fragments arranged by size:
1620 798 611 494 419 326 190 93
74 62 37
BspHI T'CATG_A
Cuts at: 3156 3156
Size: 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1774 1988 2290 2671 1774
Size: 214 302 381 3827
Fragments arranged by size:
3827 381 302 214
BsrBI CCG'CTC
Cuts at: 50 121 3016 3070 4554 4719 50
Size: 71 2895 54 1484 165 55
Fragments arranged by size:
2895 1484 165 71 55 54
BsrDI GCAATG_nn'
Cuts at: 1247 2636 1247
Size: 1389 3335
BsrFI r'CCGG_y
Cuts at: 168 2722 2903 168
Size: 2554 181 1989
Fragments arranged by size:
2554 1989 181
BssSI C'ACGA_G
Cuts at: 2995 3703 2995
Size: 708 4016
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 2101 1033
Size: 1068 3656
BstBI TT'CG_AA
Cuts at: 3085 3085
Size: 4724
BstYI r'GATC_y
Cuts at: 1 237 1016 1175 1749 2216 2239 2574 2820
Size: 236 779 159 574 467 23 335 246
Cuts at: 2820 3126 3138 3224 4276 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 779 574 467 449 335 306 246
236 159 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1441 1441
Size: 4724
BtgI C'CryG_G
Cuts at: 524 524
Size: 4724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2674 2855 3018 2674
Size: 181 163 4380
Fragments arranged by size:
4380 181 163
BtsI GCAGTG_nn'
Cuts at: 166 1967 166
Size: 1801 2923
DraI TTT'AAA
Cuts at: 1723 1723
Size: 4724
DrdI GACnn_nn'nnGTC
Cuts at: 2430 3774 2430
Size: 1344 3380
EaeI y'GGCC_r
Cuts at: 905 1715 2309 2483 2874 2901 4246 905
Size: 810 594 174 391 27 1345 1383
Fragments arranged by size:
1383 1345 810 594 391 174 27
EagI C'GGCC_G
Cuts at: 2309 2309
Size: 4724
EarI CTCTTCn'nnn_
Cuts at: 913 1042 1165 2747 2957 913
Size: 129 123 1582 210 2680
Fragments arranged by size:
2680 1582 210 129 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 287 2932 3674 3820 4534 287
Size: 2645 742 146 714 477
Fragments arranged by size:
2645 742 714 477 146
EcoICRI GAG'CTC
Cuts at: 1736 1736
Size: 4724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 656 674 1042 1356 1552 2549 2981 3334
Size: 123 18 368 314 196 997 432 353
Cuts at: 3334 3996 533
Size: 662 1261
Fragments arranged by size:
1261 997 662 432 368 353 314 196
123 18
EcoNI CCTnn'n_nnAGG
Cuts at: 382 382
Size: 4724
EcoO109I rG'GnC_Cy
Cuts at: 458 459 530 669 1069 1476 1503 1863 458
Size: 1 71 139 400 407 27 360 3319
Fragments arranged by size:
3319 407 400 360 139 71 27 1
EcoRI G'AATT_C
Cuts at: 1728 1728
Size: 4724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 807 2743 807
Size: 1936 2788
FspI TGC'GCA
Cuts at: 2505 2505
Size: 4724
HaeII r_GCGC'y
Cuts at: 864 2158 2406 3636 4407 864
Size: 1294 248 1230 771 1181
Fragments arranged by size:
1294 1230 1181 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 727 759 1274 1306 1378 1410 2566
Size: 32 346 32 515 32 72 32 1156
Cuts at: 2566 2596 2598 2628 349
Size: 30 2 30 2445
Fragments arranged by size:
2445 1156 515 346 72 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1763 1763
Size: 4724
KasI G'GCGC_C
Cuts at: 2154 2402 2154
Size: 248 4476
KpnI G_GTAC'C
Cuts at: 1744 1744
Size: 4724
MluI A'CGCG_T
Cuts at: 4157 4157
Size: 4724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1508 3483 3667 1508
Size: 1975 184 2565
Fragments arranged by size:
2565 1975 184
MscI TGG'CCA
Cuts at: 907 2485 907
Size: 1578 3146
MslI CAynn'nnrTG
Cuts at: 913 1414 1690 2713 2840 913
Size: 501 276 1023 127 2797
Fragments arranged by size:
2797 1023 501 276 127
MspA1I CmG'CkG
Cuts at: 144 247 335 770 867 875 948 1135 1835
Size: 103 88 435 97 8 73 187 700
Cuts at: 1835 2149 2509 3293 3538 144
Size: 314 360 784 245 1330
Fragments arranged by size:
1330 784 700 435 360 314 245 187
103 97 88 73 8
NaeI GCC'GGC
Cuts at: 2905 2905
Size: 4724
NarI GG'CG_CC
Cuts at: 2155 2403 2155
Size: 248 4476
NcoI C'CATG_G
Cuts at: 524 524
Size: 4724
NgoMIV G'CCGG_C
Cuts at: 2903 2903
Size: 4724
NsiI A_TGCA'T
Cuts at: 194 1527 194
Size: 1333 3391
NspI r_CATG'y
Cuts at: 151 1777 2277 3880 151
Size: 1626 500 1603 995
Fragments arranged by size:
1626 1603 995 500
PasI CC'CwG_GG
Cuts at: 384 613 700 384
Size: 229 87 4408
Fragments arranged by size:
4408 229 87
PciI A'CATG_T
Cuts at: 3876 3876
Size: 4724
PflMI CCAn_nnn'nTGG
Cuts at: 113 445 1713 113
Size: 332 1268 3124
Fragments arranged by size:
3124 1268 332
PfoI T'CCnGG_A
Cuts at: 1019 1019
Size: 4724
PmeI GTTT'AAAC
Cuts at: 1723 1723
Size: 4724
PmlI CAC'GTG
Cuts at: 1314 1314
Size: 4724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 4692
PpuMI rG'GwC_Cy
Cuts at: 530 1069 1503 530
Size: 539 434 3751
Fragments arranged by size:
3751 539 434
PspOMI G'GGCC_C
Cuts at: 458 458
Size: 4724
PstI C_TGCA'G
Cuts at: 1771 2456 1771
Size: 685 4039
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4724
PvuII CAG'CTG
Cuts at: 770 1135 2149 2509 770
Size: 365 1014 360 2985
Fragments arranged by size:
2985 1014 365 360
RsrII CG'GwC_CG
Cuts at: 2919 2919
Size: 4724
SacI G_AGCT'C
Cuts at: 1738 1738
Size: 4724
SalI G'TCGA_C
Cuts at: 1761 1761
Size: 4724
SanDI GG'GwC_CC
Cuts at: 530 530
Size: 4724
SapI GCTCTTCn'nnn_
Cuts at: 2747 2957 2747
Size: 210 4514
SbfI CC_TGCA'GG
Cuts at: 1771 1771
Size: 4724
ScaI AGT'ACT
Cuts at: 883 4241 883
Size: 3358 1366
SfcI C'TryA_G
Cuts at: 33 1767 2452 3420 3611 33
Size: 1734 685 968 191 1146
Fragments arranged by size:
1734 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2156 2404 2156
Size: 248 4476
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
SmaI CCC'GGG
Cuts at: 1746 1746
Size: 4724
SmlI C'TyrA_G
Cuts at: 1621 1696 1879 2794 3508 3770 1621
Size: 75 183 915 714 262 2575
Fragments arranged by size:
2575 915 714 262 183 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4724
SphI G_CATG'C
Cuts at: 151 1777 2277 151
Size: 1626 500 2598
Fragments arranged by size:
2598 1626 500
StyI C'CwwG_G
Cuts at: 444 524 1472 1858 2212 444
Size: 80 948 386 354 2956
Fragments arranged by size:
2956 948 386 354 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1352 2307 2440 2899 3108 1352
Size: 955 133 459 209 2968
Fragments arranged by size:
2968 955 459 209 133
TatI w'GTAC_w
Cuts at: 881 4239 881
Size: 3358 1366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1174 1206 4241 4273 1174
Size: 32 3035 32 1625
Fragments arranged by size:
3035 1625 32 32
Tth111I GACn'n_nGTC
Cuts at: 2521 2521
Size: 4724
XbaI T'CTAG_A
Cuts at: 1629 1755 1629
Size: 126 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2219 2219
Size: 4724
XmaI C'CCGG_G
Cuts at: 1744 1744
Size: 4724
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
AscI AvrII BcgI BglI BmgBI BmtI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI