Restriction map for
pF1KB4082
(Circular) (Six-base) MAPSORT of: pF1KB4082.seq Check: 4197 from: 1 to: 5795
pF1KB4082 5795 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 704 704
Size: 5795
AccI GT'mk_AC
Cuts at: 1021 1475 2833 1021
Size: 454 1358 3983
Fragments arranged by size:
3983 1358 454
Acc65I G'GTAC_C
Cuts at: 2811 2811
Size: 5795
AclI AA'CG_TT
Cuts at: 5635 5635
Size: 5795
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 2132 3620 4052 4799 5067 1016
Size: 1116 1488 432 747 268 1744
Fragments arranged by size:
1744 1488 1116 747 432 268
AfeI AGC'GCT
Cuts at: 255 964 2559 255
Size: 709 1595 3491
Fragments arranged by size:
3491 1595 709
AflIII A'CryG_T
Cuts at: 1500 2762 3147 4251 5228 1500
Size: 1262 385 1104 977 2067
Fragments arranged by size:
2067 1262 1104 977 385
AgeI A'CCGG_T
Cuts at: 4183 4183
Size: 5795
AhdI GACnn_n'nnGTC
Cuts at: 429 429
Size: 5795
AlwNI CAG_nnn'CTG
Cuts at: 3100 4667 3100
Size: 1567 4228
ApaI G_GGCC'C
Cuts at: 465 465
Size: 5795
ApaLI G'TGCA_C
Cuts at: 4565 4565
Size: 5795
ApoI r'AATT_y
Cuts at: 709 988 1912 2173 2641 2799 5148 5404 709
Size: 279 924 261 468 158 2349 256 1100
Fragments arranged by size:
2349 1100 924 468 279 261 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 5795
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5795
AvaI C'yCGr_G
Cuts at: 390 2276 2815 390
Size: 1886 539 3370
Fragments arranged by size:
3370 1886 539
AvrII C'CTAG_G
Cuts at: 813 813
Size: 5795
BamHI G'GATC_C
Cuts at: 1557 1752 2820 1557
Size: 195 1068 4532
Fragments arranged by size:
4532 1068 195
BanI G'GyrC_C
Cuts at: 1948 2811 3225 3473 3508 1948
Size: 863 414 248 35 4235
Fragments arranged by size:
4235 863 414 248 35
BanII G_rGCy'C
Cuts at: 465 1179 2809 3839 465
Size: 714 1630 1030 2421
Fragments arranged by size:
2421 1630 1030 714
BbeI G_GCGC'C
Cuts at: 3229 3477 3229
Size: 248 5547
BbsI GAAGACnn'nnnn_
Cuts at: 144 553 1865 2120 144
Size: 409 1312 255 3819
Fragments arranged by size:
3819 1312 409 255
BciVI GTATCCnnnnn_n'
Cuts at: 3693 4454 5795 3693
Size: 761 1341 3693
Fragments arranged by size:
3693 1341 761
BclI T'GATC_A
Cuts at: 2033 3315 2033
Size: 1282 4513
BfrBI ATG'CAT
Cuts at: 891 891
Size: 5795
BglII A'GATC_T
Cuts at: 3310 5347 3310
Size: 2037 3758
BlpI GC'TnA_GC
Cuts at: 1128 2421 2907 1128
Size: 1293 486 4016
Fragments arranged by size:
4016 1293 486
Bme1580I G_kGCm'C
Cuts at: 465 1148 3420 3513 4569 465
Size: 683 2272 93 1056 1691
Fragments arranged by size:
2272 1691 1056 683 93
BmrI ACTGGGnnnn_n'
Cuts at: 378 3007 3088 3423 5567 378
Size: 2629 81 335 2144 606
Fragments arranged by size:
2629 2144 606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4162 4162
Size: 5795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1139 1170 2335 1139
Size: 31 1165 4599
Fragments arranged by size:
4599 1165 31
Bpu10I CC'TnA_GC
Cuts at: 2569 2569
Size: 5795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 2971 3850 4342 4640 810
Size: 2161 879 492 298 1965
Fragments arranged by size:
2161 1965 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5499 5499
Size: 5795
BsaAI yAC'GTr
Cuts at: 330 3148 330
Size: 2818 2977
BsaBI GATnn'nnATC
Cuts at: 6 3309 5346 6
Size: 3303 2037 455
Fragments arranged by size:
3303 2037 455
BsaHI Gr'CG_yC
Cuts at: 701 3226 3474 4176 5672 701
Size: 2525 248 702 1496 824
Fragments arranged by size:
2525 1496 824 702 248
BsaWI w'CCGG_w
Cuts at: 2158 2984 3505 4183 4457 4604 5015 2158
Size: 826 521 678 274 147 411 2938
Fragments arranged by size:
2938 826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1098 1128 1793 1823 1098
Size: 30 665 30 5070
Fragments arranged by size:
5070 665 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1095 1095
Size: 5795
BseYI C'CCAG_C
Cuts at: 534 965 3220 3978 4555 5051 534
Size: 431 2255 758 577 496 1278
Fragments arranged by size:
2255 1278 758 577 496 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 292 451 532 262
Size: 30 159 81 5525
Fragments arranged by size:
5525 159 81 30
BsiEI CG_ry'CG
Cuts at: 104 3337 3383 4591 104
Size: 3233 46 1208 1308
Fragments arranged by size:
3233 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 143 251 1179 2809 3587 3777 4569 143
Size: 108 928 1630 778 190 792 1369
Fragments arranged by size:
1630 1369 928 792 778 190 108
BsmI GAATG_Cn'
Cuts at: 1011 1011
Size: 5795
Bsp1286I G_dGCh'C
Cuts at: 143 251 465 1148 1179 2809 3420 3513 3587
Size: 108 214 683 31 1630 611 93 74
Cuts at: 3587 3777 3839 4569 143
Size: 190 62 730 1369
Fragments arranged by size:
1630 1369 730 683 611 214 190 108
93 74 62 31
BspEI T'CCGG_A
Cuts at: 5015 5015
Size: 5795
BspHI T'CATG_A
Cuts at: 227 1682 1703 4971 227
Size: 1455 21 3268 1051
Fragments arranged by size:
3268 1455 1051 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1820 2845 3059 3361 3742 1820
Size: 1025 214 302 381 3873
Fragments arranged by size:
3873 1025 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4087 4141 5625 5790 50
Size: 4037 54 1484 165 55
Fragments arranged by size:
4037 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1356 2580 3707 1356
Size: 1224 1127 3444
Fragments arranged by size:
3444 1224 1127
BsrFI r'CCGG_y
Cuts at: 3793 3974 4183 3793
Size: 181 209 5405
Fragments arranged by size:
5405 209 181
BsrGI T'GTAC_A
Cuts at: 1418 1418
Size: 5795
BssSI C'ACGA_G
Cuts at: 1877 4066 4424 1877
Size: 2189 358 3248
Fragments arranged by size:
3248 2189 358
BstAPI GCAn_nnn'nTGC
Cuts at: 138 1138 3172 138
Size: 1000 2034 2761
Fragments arranged by size:
2761 2034 1000
BstBI TT'CG_AA
Cuts at: 4156 4156
Size: 5795
BstXI CCAn_nnnn'nTGG
Cuts at: 2628 2628
Size: 5795
BstYI r'GATC_y
Cuts at: 1 1557 1752 2185 2820 3287 3310 3645 3891
Size: 1556 195 433 635 467 23 335 246
Cuts at: 3891 4903 4989 5001 5347 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1556 1012 635 467 449 433 346 335
246 195 86 23 12
BstZ17I GTA'TAC
Cuts at: 1022 1022
Size: 5795
Bsu36I CC'TnA_GG
Cuts at: 1078 1078
Size: 5795
BtgI C'CryG_G
Cuts at: 107 107
Size: 5795
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3745 3926 4089 3745
Size: 181 163 5451
Fragments arranged by size:
5451 181 163
BtsI GCAGTG_nn'
Cuts at: 656 3038 656
Size: 2382 3413
ClaI AT'CG_AT
Cuts at: 1256 1256
Size: 5795
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 665 700 665
Size: 35 5760
DraI TTT'AAA
Cuts at: 2794 2794
Size: 5795
DrdI GACnn_nn'nnGTC
Cuts at: 772 3501 4359 772
Size: 2729 858 2208
Fragments arranged by size:
2729 2208 858
EaeI y'GGCC_r
Cuts at: 110 2618 3380 3554 3945 3972 5317 110
Size: 2508 762 174 391 27 1345 588
Fragments arranged by size:
2508 1345 762 588 391 174 27
EagI C'GGCC_G
Cuts at: 3380 3380
Size: 5795
EarI CTCTTCn'nnn_
Cuts at: 3818 4028 3818
Size: 210 5585
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4003 4313 4459 5605 4003
Size: 310 146 1146 4193
Fragments arranged by size:
4193 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1177 2807 1177
Size: 1630 4165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1139 1170 2132 2335 3620 4052 4799 5067
Size: 123 31 962 203 1285 432 747 268
Cuts at: 5067 1016
Size: 1744
Fragments arranged by size:
1744 1285 962 747 432 268 203 123
31
EcoO109I rG'GnC_Cy
Cuts at: 461 462 2147 2934 461
Size: 1 1685 787 3322
Fragments arranged by size:
3322 1685 787 1
EcoRI G'AATT_C
Cuts at: 709 988 2641 2799 709
Size: 279 1653 158 3705
Fragments arranged by size:
3705 1653 279 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 774 2322 3814 774
Size: 1548 1492 2755
Fragments arranged by size:
2755 1548 1492
FspI TGC'GCA
Cuts at: 3576 3576
Size: 5795
HaeII r_GCGC'y
Cuts at: 257 551 966 2561 3229 3477 4499 5478 257
Size: 294 415 1595 668 248 1022 979 574
Fragments arranged by size:
1595 1022 979 668 574 415 294 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 608 640 1345 1377 2079 2111 2544
Size: 32 161 32 705 32 702 32 433
Cuts at: 2544 2576 3637 3667 3669 3699 415
Size: 32 1061 30 2 30 2511
Fragments arranged by size:
2511 1061 705 702 433 161 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 927 2321 2834 927
Size: 1394 513 3888
Fragments arranged by size:
3888 1394 513
HindIII A'AGCT_T
Cuts at: 1064 1064
Size: 5795
KasI G'GCGC_C
Cuts at: 3225 3473 3225
Size: 248 5547
KpnI G_GTAC'C
Cuts at: 2815 2815
Size: 5795
MluI A'CGCG_T
Cuts at: 5228 5228
Size: 5795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1986 2583 4466 4650 1986
Size: 597 1883 184 3131
Fragments arranged by size:
3131 1883 597 184
MscI TGG'CCA
Cuts at: 112 2620 3556 112
Size: 2508 936 2351
Fragments arranged by size:
2508 2351 936
MslI CAynn'nnrTG
Cuts at: 526 1258 1384 2626 3784 3911 526
Size: 732 126 1242 1158 127 2410
Fragments arranged by size:
2410 1242 1158 732 127 126
MspA1I CmG'CkG
Cuts at: 2233 2906 3220 3580 4593 4838 2233
Size: 673 314 360 1013 245 3190
Fragments arranged by size:
3190 1013 673 360 314 245
NaeI GCC'GGC
Cuts at: 3976 3976
Size: 5795
NarI GG'CG_CC
Cuts at: 3226 3474 3226
Size: 248 5547
NcoI C'CATG_G
Cuts at: 107 107
Size: 5795
NdeI CA'TA_TG
Cuts at: 1410 2142 1410
Size: 732 5063
NgoMIV G'CCGG_C
Cuts at: 3974 3974
Size: 5795
NsiI A_TGCA'T
Cuts at: 893 893
Size: 5795
NspI r_CATG'y
Cuts at: 846 1504 2848 3348 4255 846
Size: 658 1344 500 907 2386
Fragments arranged by size:
2386 1344 907 658 500
PciI A'CATG_T
Cuts at: 1500 4251 1500
Size: 2751 3044
PfoI T'CCnGG_A
Cuts at: 1102 2536 1102
Size: 1434 4361
PmeI GTTT'AAAC
Cuts at: 2794 2794
Size: 5795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 4934
Size: 32 5763
PpuMI rG'GwC_Cy
Cuts at: 2147 2147
Size: 5795
PspOMI G'GGCC_C
Cuts at: 461 461
Size: 5795
PstI C_TGCA'G
Cuts at: 2842 3527 2842
Size: 685 5110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5795
PvuII CAG'CTG
Cuts at: 3220 3580 3220
Size: 360 5435
RsrII CG'GwC_CG
Cuts at: 3990 3990
Size: 5795
SacI G_AGCT'C
Cuts at: 1179 2809 1179
Size: 1630 4165
SalI G'TCGA_C
Cuts at: 2832 2832
Size: 5795
SanDI GG'GwC_CC
Cuts at: 2147 2147
Size: 5795
SapI GCTCTTCn'nnn_
Cuts at: 3818 4028 3818
Size: 210 5585
SbfI CC_TGCA'GG
Cuts at: 2842 2842
Size: 5795
ScaI AGT'ACT
Cuts at: 5312 5312
Size: 5795
SfcI C'TryA_G
Cuts at: 33 2838 3523 4516 4707 33
Size: 2805 685 993 191 1121
Fragments arranged by size:
2805 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3227 3475 3227
Size: 248 5547
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5795
SmaI CCC'GGG
Cuts at: 2817 2817
Size: 5795
SmlI C'TyrA_G
Cuts at: 390 825 2950 3865 4357 4619 390
Size: 435 2125 915 492 262 1566
Fragments arranged by size:
2125 1566 915 492 435 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5795
SphI G_CATG'C
Cuts at: 2848 3348 2848
Size: 500 5295
SspI AAT'ATT
Cuts at: 1215 1922 1215
Size: 707 5088
StyI C'CwwG_G
Cuts at: 107 813 1943 2291 2528 2929 3283 107
Size: 706 1130 348 237 401 354 2619
Fragments arranged by size:
2619 1130 706 401 354 348 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 957 1936 2710 3378 3511 3970 4179 957
Size: 979 774 668 133 459 209 2573
Fragments arranged by size:
2573 979 774 668 459 209 133
TatI w'GTAC_w
Cuts at: 1194 1418 2333 5310 1194
Size: 224 915 2977 1679
Fragments arranged by size:
2977 1679 915 224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 1098 1130 1533 1565 5312 5344 328
Size: 32 738 32 403 32 3747 32 779
Fragments arranged by size:
3747 779 738 403 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3592 3592
Size: 5795
XbaI T'CTAG_A
Cuts at: 2826 2826
Size: 5795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3290 3290
Size: 5795
XhoI C'TCGA_G
Cuts at: 390 390
Size: 5795
XmaI C'CCGG_G
Cuts at: 2815 2815
Size: 5795
XmnI GAAnn'nnTTC
Cuts at: 310 310
Size: 5795
ZraI GAC'GTC
Cuts at: 702 702
Size: 5795
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AleI AlfI AloI AscI BaeI BbvCI
BcgI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI