Restriction map for
pF1KB4084
(Circular) (Six-base) MAPSORT of: pF1KB4084.seq Check: 2421 from: 1 to: 6020
pF1KB4084 6020 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2931 2931
Size: 6020
AccI GT'mk_AC
Cuts at: 3058 3058
Size: 6020
Acc65I G'GTAC_C
Cuts at: 3036 3036
Size: 6020
AclI AA'CG_TT
Cuts at: 5860 5860
Size: 6020
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1538 2242 2583 3845 4277 4630 5292 1114
Size: 424 704 341 1262 432 353 662 1842
Fragments arranged by size:
1842 1262 704 662 432 424 353 341
AflIII A'CryG_T
Cuts at: 551 1383 2144 3372 5172 5453 551
Size: 832 761 1228 1800 281 1118
Fragments arranged by size:
1800 1228 1118 832 761 281
AhdI GACnn_n'nnGTC
Cuts at: 393 1645 393
Size: 1252 4768
AleI CACnn'nnGTG
Cuts at: 2888 2888
Size: 6020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 572 1066 572
Size: 494 5526
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1373 1405 1373
Size: 32 5988
AlwNI CAG_nnn'CTG
Cuts at: 232 975 3325 4763 232
Size: 743 2350 1438 1489
Fragments arranged by size:
2350 1489 1438 743
ApaI G_GGCC'C
Cuts at: 924 2912 924
Size: 1988 4032
ApaLI G'TGCA_C
Cuts at: 188 1728 2323 2500 4858 188
Size: 1540 595 177 2358 1350
Fragments arranged by size:
2358 1540 1350 595 177
ApoI r'AATT_y
Cuts at: 1137 2179 2305 2631 3024 5373 5629 1137
Size: 1042 126 326 393 2349 256 1528
Fragments arranged by size:
2349 1528 1042 393 326 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 6020
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6020
AvaI C'yCGr_G
Cuts at: 808 850 2654 3040 808
Size: 42 1804 386 3788
Fragments arranged by size:
3788 1804 386 42
AvrII C'CTAG_G
Cuts at: 361 361
Size: 6020
BamHI G'GATC_C
Cuts at: 804 2249 3045 804
Size: 1445 796 3779
Fragments arranged by size:
3779 1445 796
BanI G'GyrC_C
Cuts at: 332 3036 3450 3698 3733 332
Size: 2704 414 248 35 2619
Fragments arranged by size:
2704 2619 414 248 35
BanII G_rGCy'C
Cuts at: 223 924 2912 3034 4064 223
Size: 701 1988 122 1030 2179
Fragments arranged by size:
2179 1988 1030 701 122
BbeI G_GCGC'C
Cuts at: 3454 3702 3454
Size: 248 5772
BbsI GAAGACnn'nnnn_
Cuts at: 581 1297 1380 1962 3003 581
Size: 716 83 582 1041 3598
Fragments arranged by size:
3598 1041 716 582 83
BbvCI CC'TCA_GC
Cuts at: 857 857
Size: 6020
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 449 1972 2006 415
Size: 34 1523 34 4429
Fragments arranged by size:
4429 1523 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2186 3918 4974 6020 2186
Size: 1732 1056 1046 2186
Fragments arranged by size:
2186 1732 1056 1046
BclI T'GATC_A
Cuts at: 1605 3540 1605
Size: 1935 4085
BglI GCCn_nnn'nGGC
Cuts at: 928 928
Size: 6020
BglII A'GATC_T
Cuts at: 1913 3535 5572 1913
Size: 1622 2037 2361
Fragments arranged by size:
2361 2037 1622
BlpI GC'TnA_GC
Cuts at: 1783 3132 1783
Size: 1349 4671
Bme1580I G_kGCm'C
Cuts at: 192 924 1439 1732 1958 2327 2497 2504 2571
Size: 732 515 293 226 369 170 7 67
Cuts at: 2571 2912 3645 3738 4862 192
Size: 341 733 93 1124 1350
Fragments arranged by size:
1350 1124 733 732 515 369 341 293
226 170 93 67 7
BmgBI CAC'GTC
Cuts at: 444 1968 444
Size: 1524 4496
BmrI ACTGGGnnnn_n'
Cuts at: 1295 3232 3313 3648 5792 1295
Size: 1937 81 335 2144 1523
Fragments arranged by size:
2144 1937 1523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4387 4387
Size: 6020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 933 1237 2539 228
Size: 705 304 1302 3709
Fragments arranged by size:
3709 1302 705 304
Bpu10I CC'TnA_GC
Cuts at: 592 857 592
Size: 265 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 4075 4789 5087 3196
Size: 879 714 298 4129
Fragments arranged by size:
4129 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 987 1024 1331 5724 987
Size: 37 307 4393 1283
Fragments arranged by size:
4393 1283 307 37
BsaAI yAC'GTr
Cuts at: 552 1611 3373 552
Size: 1059 1762 3199
Fragments arranged by size:
3199 1762 1059
BsaBI GATnn'nnATC
Cuts at: 6 1084 3534 5571 6
Size: 1078 2450 2037 455
Fragments arranged by size:
2450 2037 1078 455
BsaHI Gr'CG_yC
Cuts at: 1811 3451 3699 4401 5897 1811
Size: 1640 248 702 1496 1934
Fragments arranged by size:
1934 1640 1496 702 248
BsaWI w'CCGG_w
Cuts at: 989 3209 3730 4408 4819 4966 5240 989
Size: 2220 521 678 411 147 274 1769
Fragments arranged by size:
2220 1769 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 934 1183 835
Size: 99 249 5672
Fragments arranged by size:
5672 249 99
BseYI C'CCAG_C
Cuts at: 436 997 1393 1719 2585 2710 3445 4203 4868
Size: 561 396 326 866 125 735 758 665
Cuts at: 4868 5276 436
Size: 408 1180
Fragments arranged by size:
1180 866 758 735 665 561 408 396
326 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1128 1824 230
Size: 898 696 4426
Fragments arranged by size:
4426 898 696
BsiEI CG_ry'CG
Cuts at: 104 3562 3608 4838 104
Size: 3458 46 1230 1286
Fragments arranged by size:
3458 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 192 793 944 1732 2327 2504 3034 3812 4002
Size: 601 151 788 595 177 530 778 190
Cuts at: 4002 4862 192
Size: 860 1350
Fragments arranged by size:
1350 860 788 778 601 595 530 190
177 151
BsiWI C'GTAC_G
Cuts at: 2160 2160
Size: 6020
BsmBI CGTCTCn'nnnn_
Cuts at: 401 2674 401
Size: 2273 3747
Bsp1286I G_dGCh'C
Cuts at: 192 223 793 924 944 1439 1732 1958 2327
Size: 31 570 131 20 495 293 226 369
Cuts at: 2327 2497 2504 2571 2912 3034 3645 3738 3812
Size: 170 7 67 341 122 611 93 74
Cuts at: 3812 4002 4064 4862 192
Size: 190 62 798 1350
Fragments arranged by size:
1350 798 611 570 495 369 341 293
226 190 170 131 122 93 74 67
62 31 20 7
BspEI T'CCGG_A
Cuts at: 989 989
Size: 6020
BspHI T'CATG_A
Cuts at: 1755 1853 2675 4452 1755
Size: 98 822 1777 3323
Fragments arranged by size:
3323 1777 822 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1325 2588 2931 3070 3284 3586 3967 1325
Size: 1263 343 139 214 302 381 3378
Fragments arranged by size:
3378 1263 381 343 302 214 139
BsrBI CCG'CTC
Cuts at: 50 4312 4366 5850 6015 50
Size: 4262 54 1484 165 55
Fragments arranged by size:
4262 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3932 3932
Size: 6020
BsrFI r'CCGG_y
Cuts at: 299 1279 4018 4199 299
Size: 980 2739 181 2120
Fragments arranged by size:
2739 2120 980 181
BssHII G'CGCG_C
Cuts at: 2946 2946
Size: 6020
BssSI C'ACGA_G
Cuts at: 4291 4999 4291
Size: 708 5312
BstAPI GCAn_nnn'nTGC
Cuts at: 2580 3397 2580
Size: 817 5203
BstBI TT'CG_AA
Cuts at: 4381 4381
Size: 6020
BstEII G'GTnAC_C
Cuts at: 1008 1008
Size: 6020
BstXI CCAn_nnnn'nTGG
Cuts at: 436 1181 1821 2086 2517 2688 436
Size: 745 640 265 431 171 3768
Fragments arranged by size:
3768 745 640 431 265 171
BstYI r'GATC_y
Cuts at: 1 804 1913 2249 3045 3512 3535 3870 4116
Size: 803 1109 336 796 467 23 335 246
Cuts at: 4116 4422 4434 4520 5572 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1109 1052 803 796 467 449 336 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 197 544 197
Size: 347 5673
BtgI C'CryG_G
Cuts at: 107 328 1004 1424 2951 107
Size: 221 676 420 1527 3176
Fragments arranged by size:
3176 1527 676 420 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1350 3970 4151 4314 1350
Size: 2620 181 163 3056
Fragments arranged by size:
3056 2620 181 163
BtsI GCAGTG_nn'
Cuts at: 190 213 960 3263 190
Size: 23 747 2303 2947
Fragments arranged by size:
2947 2303 747 23
ClaI AT'CG_AT
Cuts at: 666 666
Size: 6020
DraI TTT'AAA
Cuts at: 3019 3019
Size: 6020
DraIII CAC_nnn'GTG
Cuts at: 788 788
Size: 6020
DrdI GACnn_nn'nnGTC
Cuts at: 3726 5070 3726
Size: 1344 4676
EaeI y'GGCC_r
Cuts at: 110 129 1183 3605 3779 4170 4197 5542 110
Size: 19 1054 2422 174 391 27 1345 588
Fragments arranged by size:
2422 1345 1054 588 391 174 27 19
EagI C'GGCC_G
Cuts at: 3605 3605
Size: 6020
EarI CTCTTCn'nnn_
Cuts at: 266 677 985 1483 1586 1730 3007 4043 4253
Size: 411 308 498 103 144 1277 1036 210
Cuts at: 4253 266
Size: 2033
Fragments arranged by size:
2033 1277 1036 498 411 308 210 144
103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1461 4228 4970 5116 5830 1461
Size: 2767 742 146 714 1651
Fragments arranged by size:
2767 1651 742 714 146
EcoICRI GAG'CTC
Cuts at: 3032 3032
Size: 6020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 933 1114 1237 1538 2242 2539 2583 3845
Size: 705 181 123 301 704 297 44 1262
Cuts at: 3845 4277 4630 5292 228
Size: 432 353 662 956
Fragments arranged by size:
1262 956 705 704 662 432 353 301
297 181 123 44
EcoNI CCTnn'n_nnAGG
Cuts at: 200 850 200
Size: 650 5370
EcoO109I rG'GnC_Cy
Cuts at: 448 488 540 921 1899 2909 2994 3159 448
Size: 40 52 381 978 1010 85 165 3309
Fragments arranged by size:
3309 1010 978 381 165 85 52 40
EcoRI G'AATT_C
Cuts at: 1137 2179 3024 1137
Size: 1042 845 4133
Fragments arranged by size:
4133 1042 845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4039 4039
Size: 6020
FspI TGC'GCA
Cuts at: 3801 3801
Size: 6020
HaeII r_GCGC'y
Cuts at: 3454 3702 4932 5703 3454
Size: 248 1230 771 3771
Fragments arranged by size:
3771 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 789 821 1159 1191 1218 1233 1250
Size: 32 378 32 338 32 27 15 17
Cuts at: 1250 1265 1309 1341 1590 1622 1631 1663 2012
Size: 15 44 32 249 32 9 32 349
Cuts at: 2012 2044 3862 3892 3894 3924 379
Size: 32 1818 30 2 30 2475
Fragments arranged by size:
2475 1818 378 349 338 249 44 32
32 32 32 32 32 32 30 30
27 17 15 15 9 2
HincII GTy'rAC
Cuts at: 912 1487 1503 1938 2808 3059 912
Size: 575 16 435 870 251 3873
Fragments arranged by size:
3873 870 575 435 251 16
HindIII A'AGCT_T
Cuts at: 1546 1546
Size: 6020
HpaI GTT'AAC
Cuts at: 1487 1487
Size: 6020
KasI G'GCGC_C
Cuts at: 3450 3698 3450
Size: 248 5772
KpnI G_GTAC'C
Cuts at: 3040 3040
Size: 6020
MluI A'CGCG_T
Cuts at: 1383 5453 1383
Size: 4070 1950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1227 2049 4779 4963 1227
Size: 822 2730 184 2284
Fragments arranged by size:
2730 2284 822 184
MscI TGG'CCA
Cuts at: 112 131 1185 3781 112
Size: 19 1054 2596 2351
Fragments arranged by size:
2596 2351 1054 19
MslI CAynn'nnrTG
Cuts at: 1048 1819 2084 2888 4009 4136 1048
Size: 771 265 804 1121 127 2932
Fragments arranged by size:
2932 1121 804 771 265 127
MspA1I CmG'CkG
Cuts at: 140 185 636 1224 1782 2365 3131 3445 3805
Size: 45 451 588 558 583 766 314 360
Cuts at: 3805 4589 4834 140
Size: 784 245 1326
Fragments arranged by size:
1326 784 766 588 583 558 451 360
314 245 45
NaeI GCC'GGC
Cuts at: 1281 4201 1281
Size: 2920 3100
NarI GG'CG_CC
Cuts at: 3451 3699 3451
Size: 248 5772
NcoI C'CATG_G
Cuts at: 107 1424 2951 107
Size: 1317 1527 3176
Fragments arranged by size:
3176 1527 1317
NgoMIV G'CCGG_C
Cuts at: 1279 4199 1279
Size: 2920 3100
NspI r_CATG'y
Cuts at: 1713 2539 3073 3573 5176 1713
Size: 826 534 500 1603 2557
Fragments arranged by size:
2557 1603 826 534 500
PciI A'CATG_T
Cuts at: 5172 5172
Size: 6020
PflMI CCAn_nnn'nTGG
Cuts at: 302 1425 302
Size: 1123 4897
PfoI T'CCnGG_A
Cuts at: 244 2128 244
Size: 1884 4136
PmeI GTTT'AAAC
Cuts at: 3019 3019
Size: 6020
PmlI CAC'GTG
Cuts at: 552 1611 552
Size: 1059 4961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 5988
PpuMI rG'GwC_Cy
Cuts at: 448 488 540 1899 448
Size: 40 52 1359 4569
Fragments arranged by size:
4569 1359 52 40
PshAI GACnn'nnGTC
Cuts at: 140 446 140
Size: 306 5714
PspOMI G'GGCC_C
Cuts at: 920 2908 920
Size: 1988 4032
PspXI vC'TCGA_Gb
Cuts at: 2654 2654
Size: 6020
PstI C_TGCA'G
Cuts at: 177 3067 3752 177
Size: 2890 685 2445
Fragments arranged by size:
2890 2445 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6020
PvuII CAG'CTG
Cuts at: 140 185 1224 1782 3445 3805 140
Size: 45 1039 558 1663 360 2355
Fragments arranged by size:
2355 1663 1039 558 360 45
RsrII CG'GwC_CG
Cuts at: 4215 4215
Size: 6020
SacI G_AGCT'C
Cuts at: 3034 3034
Size: 6020
SalI G'TCGA_C
Cuts at: 3057 3057
Size: 6020
SanDI GG'GwC_CC
Cuts at: 1899 1899
Size: 6020
SapI GCTCTTCn'nnn_
Cuts at: 677 985 1586 3007 4043 4253 677
Size: 308 601 1421 1036 210 2444
Fragments arranged by size:
2444 1421 1036 601 308 210
SbfI CC_TGCA'GG
Cuts at: 3067 3067
Size: 6020
ScaI AGT'ACT
Cuts at: 2719 5537 2719
Size: 2818 3202
SfcI C'TryA_G
Cuts at: 33 173 2844 3063 3748 4716 4907 33
Size: 140 2671 219 685 968 191 1146
Fragments arranged by size:
2671 1146 968 685 219 191 140
SfoI GGC'GCC
Cuts at: 3452 3700 3452
Size: 248 5772
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6020
SmaI CCC'GGG
Cuts at: 3042 3042
Size: 6020
SmlI C'TyrA_G
Cuts at: 2654 3175 4090 4804 5066 2654
Size: 521 915 714 262 3608
Fragments arranged by size:
3608 915 714 521 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6020
SphI G_CATG'C
Cuts at: 3073 3573 3073
Size: 500 5520
StuI AGG'CCT
Cuts at: 360 2516 360
Size: 2156 3864
StyI C'CwwG_G
Cuts at: 107 361 646 1424 1635 2951 3154 3508 107
Size: 254 285 778 211 1316 203 354 2619
Fragments arranged by size:
2619 1316 778 354 285 254 211 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1978 2310 3603 3736 4195 4404 1978
Size: 332 1293 133 459 209 3594
Fragments arranged by size:
3594 1293 459 332 209 133
TatI w'GTAC_w
Cuts at: 1037 2483 2717 5535 1037
Size: 1446 234 2818 1522
Fragments arranged by size:
2818 1522 1446 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5537 5569 5537
Size: 32 5988
Tth111I GACn'n_nGTC
Cuts at: 2812 3817 2812
Size: 1005 5015
XbaI T'CTAG_A
Cuts at: 1563 3051 1563
Size: 1488 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3515 3515
Size: 6020
XhoI C'TCGA_G
Cuts at: 2654 2654
Size: 6020
XmaI C'CCGG_G
Cuts at: 3040 3040
Size: 6020
XmnI GAAnn'nnTTC
Cuts at: 1477 1477
Size: 6020
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AscI BaeI BfrBI BmtI
BsaXI BsmI BsrGI BstZ17I CspCI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PsiI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI