Restriction map for
pF1KB4089
(Circular) (Six-base) MAPSORT of: pF1KB4089.seq Check: 3960 from: 1 to: 7781
pF1KB4089 7781 bp
With 182 enzymes: SgfI *
September 19, 2018 16:07 ..
AccI GT'mk_AC
Cuts at: 282 3693 4819 282
Size: 3411 1126 3244
Fragments arranged by size:
3411 3244 1126
Acc65I G'GTAC_C
Cuts at: 4797 4797
Size: 7781
AclI AA'CG_TT
Cuts at: 3705 7621 3705
Size: 3916 3865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 811 897 1586 1769 2321 2627 2939 3686
Size: 486 86 689 183 552 306 312 747
Cuts at: 3686 4221 5606 6038 6785 7053 325
Size: 535 1385 432 747 268 1053
Fragments arranged by size:
1385 1053 747 747 689 552 535 486
432 312 306 268 183 86
AflII C'TTAA_G
Cuts at: 1327 2131 1327
Size: 804 6977
AflIII A'CryG_T
Cuts at: 561 5133 6237 7214 561
Size: 4572 1104 977 1128
Fragments arranged by size:
4572 1128 1104 977
AgeI A'CCGG_T
Cuts at: 6169 6169
Size: 7781
AhdI GACnn_n'nnGTC
Cuts at: 4370 4714 4370
Size: 344 7437
AleI CACnn'nnGTG
Cuts at: 1918 1918
Size: 7781
AlwNI CAG_nnn'CTG
Cuts at: 1792 5086 6653 1792
Size: 3294 1567 2920
Fragments arranged by size:
3294 2920 1567
ApaLI G'TGCA_C
Cuts at: 2897 6551 2897
Size: 3654 4127
ApoI r'AATT_y
Cuts at: 416 2105 2193 2371 2926 4785 7134 7390 416
Size: 1689 88 178 555 1859 2349 256 807
Fragments arranged by size:
2349 1859 1689 807 555 256 178 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 7781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7781
AvaI C'yCGr_G
Cuts at: 4801 4801
Size: 7781
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 280 313 280
Size: 33 7748
BamHI G'GATC_C
Cuts at: 4806 4806
Size: 7781
BanI G'GyrC_C
Cuts at: 4797 5211 5459 5494 4797
Size: 414 248 35 7084
Fragments arranged by size:
7084 414 248 35
BanII G_rGCy'C
Cuts at: 2474 4795 5825 2474
Size: 2321 1030 4430
Fragments arranged by size:
4430 2321 1030
BbeI G_GCGC'C
Cuts at: 5215 5463 5215
Size: 248 7533
BbsI GAAGACnn'nnnn_
Cuts at: 2615 3212 3720 3770 3906 2615
Size: 597 508 50 136 6490
Fragments arranged by size:
6490 597 508 136 50
BbvCI CC'TCA_GC
Cuts at: 2948 2948
Size: 7781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7747
BciVI GTATCCnnnnn_n'
Cuts at: 2682 5679 6440 7781 2682
Size: 2997 761 1341 2682
Fragments arranged by size:
2997 2682 1341 761
BclI T'GATC_A
Cuts at: 5301 5301
Size: 7781
BfrBI ATG'CAT
Cuts at: 1417 2995 1417
Size: 1578 6203
BglII A'GATC_T
Cuts at: 203 2983 4273 5296 7333 203
Size: 2780 1290 1023 2037 651
Fragments arranged by size:
2780 2037 1290 1023 651
BlpI GC'TnA_GC
Cuts at: 4893 4893
Size: 7781
Bme1580I G_kGCm'C
Cuts at: 2901 5406 5499 6555 2901
Size: 2505 93 1056 4127
Fragments arranged by size:
4127 2505 1056 93
BmgBI CAC'GTC
Cuts at: 4716 4716
Size: 7781
BmrI ACTGGGnnnn_n'
Cuts at: 1883 4378 4718 4993 5074 5409 7553 1883
Size: 2495 340 275 81 335 2144 2111
Fragments arranged by size:
2495 2144 2111 340 335 275 81
BmtI G_CTAG'C
Cuts at: 2908 2908
Size: 7781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3481 6148 3479
Size: 2 2667 5112
Fragments arranged by size:
5112 2667 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2705 3144 4331 2705
Size: 439 1187 6155
Fragments arranged by size:
6155 1187 439
Bpu10I CC'TnA_GC
Cuts at: 2948 2948
Size: 7781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 2642 3637 3848 3939 4957 5836 6328 6626
Size: 2311 995 211 91 1018 879 492 298
Cuts at: 6626 331
Size: 1486
Fragments arranged by size:
2311 1486 1018 995 879 492 298 211
91
BsaI GGTCTCn'nnnn_
Cuts at: 933 3726 7485 933
Size: 2793 3759 1229
Fragments arranged by size:
3759 2793 1229
BsaAI yAC'GTr
Cuts at: 1252 2734 5134 1252
Size: 1482 2400 3899
Fragments arranged by size:
3899 2400 1482
BsaBI GATnn'nnATC
Cuts at: 6 5295 7332 6
Size: 5289 2037 455
Fragments arranged by size:
5289 2037 455
BsaHI Gr'CG_yC
Cuts at: 5212 5460 6162 7658 5212
Size: 248 702 1496 5335
Fragments arranged by size:
5335 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4970 5491 6169 6443 6590 7001 4970
Size: 521 678 274 147 411 5750
Fragments arranged by size:
5750 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3267 3297 3817 3847 4286 4316 3267
Size: 30 520 30 439 30 6732
Fragments arranged by size:
6732 520 439 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1850 3711 3838 4061 4412 1850
Size: 1861 127 223 351 5219
Fragments arranged by size:
5219 1861 351 223 127
BseYI C'CCAG_C
Cuts at: 5206 5964 6541 7037 5206
Size: 758 577 496 5950
Fragments arranged by size:
5950 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 1731 1756 2767 3038 1646
Size: 85 25 1011 271 6389
Fragments arranged by size:
6389 1011 271 85 25
BsiEI CG_ry'CG
Cuts at: 104 2532 5323 5369 6577 104
Size: 2428 2791 46 1208 1308
Fragments arranged by size:
2791 2428 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2474 2901 4012 4795 5573 5763 6555 2474
Size: 427 1111 783 778 190 792 3700
Fragments arranged by size:
3700 1111 792 783 778 427 190
BsmI GAATG_Cn'
Cuts at: 3555 3555
Size: 7781
Bsp1286I G_dGCh'C
Cuts at: 2474 2901 4012 4795 5406 5499 5573 5763 5825
Size: 427 1111 783 611 93 74 190 62
Cuts at: 5825 6555 2474
Size: 730 3700
Fragments arranged by size:
3700 1111 783 730 611 427 190 93
74 62
BspEI T'CCGG_A
Cuts at: 7001 7001
Size: 7781
BspHI T'CATG_A
Cuts at: 4404 6957 4404
Size: 2553 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 167 4831 5045 5347 5728 167
Size: 4664 214 302 381 2220
Fragments arranged by size:
4664 2220 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3384 6073 6127 7611 7776 50
Size: 3334 2689 54 1484 165 55
Fragments arranged by size:
3334 2689 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3356 4244 4526 5693 3356
Size: 888 282 1167 5444
Fragments arranged by size:
5444 1167 888 282
BsrFI r'CCGG_y
Cuts at: 4752 5779 5960 6169 4752
Size: 1027 181 209 6364
Fragments arranged by size:
6364 1027 209 181
BsrGI T'GTAC_A
Cuts at: 2649 2649
Size: 7781
BssSI C'ACGA_G
Cuts at: 767 6052 6410 767
Size: 5285 358 2138
Fragments arranged by size:
5285 2138 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 5158 1669
Size: 3489 4292
BstBI TT'CG_AA
Cuts at: 4615 6142 4615
Size: 1527 6254
BstXI CCAn_nnnn'nTGG
Cuts at: 2780 4126 4731 2780
Size: 1346 605 5830
Fragments arranged by size:
5830 1346 605
BstYI r'GATC_y
Cuts at: 1 203 1734 2661 2983 4273 4806 5273 5296
Size: 202 1531 927 322 1290 533 467 23
Cuts at: 5296 5631 5877 6889 6975 6987 7333 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1531 1290 1012 927 533 467 449 346
335 322 246 202 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2666 2666
Size: 7781
BtgI C'CryG_G
Cuts at: 152 152
Size: 7781
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3596 3815 4340 5731 5912 6075 3596
Size: 219 525 1391 181 163 5302
Fragments arranged by size:
5302 1391 525 219 181 163
BtsI GCAGTG_nn'
Cuts at: 511 3336 4132 5024 511
Size: 2825 796 892 3268
Fragments arranged by size:
3268 2825 892 796
ClaI AT'CG_AT
Cuts at: 450 450
Size: 7781
DraI TTT'AAA
Cuts at: 2367 4780 2367
Size: 2413 5368
DraIII CAC_nnn'GTG
Cuts at: 1519 1519
Size: 7781
DrdI GACnn_nn'nnGTC
Cuts at: 5487 6345 5487
Size: 858 6923
EaeI y'GGCC_r
Cuts at: 1696 3963 4386 5366 5540 5931 5958 7303 1696
Size: 2267 423 980 174 391 27 1345 2174
Fragments arranged by size:
2267 2174 1345 980 423 391 174 27
EagI C'GGCC_G
Cuts at: 5366 5366
Size: 7781
EarI CTCTTCn'nnn_
Cuts at: 927 1175 1568 2579 2915 3209 3824 3863 3951
Size: 248 393 1011 336 294 615 39 88
Cuts at: 3951 5804 6014 927
Size: 1853 210 2694
Fragments arranged by size:
2694 1853 1011 615 393 336 294 248
210 88 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 5989 6299 6445 7591 199
Size: 5790 310 146 1146 389
Fragments arranged by size:
5790 1146 389 310 146
EcoICRI GAG'CTC
Cuts at: 2472 4793 2472
Size: 2321 5460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 811 897 1586 1769 2321 2627 2705 2939
Size: 486 86 689 183 552 306 78 234
Cuts at: 2939 3144 3686 4221 4331 5606 6038 6785 7053
Size: 205 542 535 110 1275 432 747 268
Cuts at: 7053 325
Size: 1053
Fragments arranged by size:
1275 1053 747 689 552 542 535 486
432 306 268 234 205 183 110 86
78
EcoO109I rG'GnC_Cy
Cuts at: 4494 4920 4494
Size: 426 7355
EcoRI G'AATT_C
Cuts at: 2105 4785 2105
Size: 2680 5101
EcoRV GAT'ATC
Cuts at: 429 1825 429
Size: 1396 6385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 647 1231 2146 3277 3421 3514 4007 5800 647
Size: 584 915 1131 144 93 493 1793 2628
Fragments arranged by size:
2628 1793 1131 915 584 493 144 93
FspI TGC'GCA
Cuts at: 5562 5562
Size: 7781
HaeII r_GCGC'y
Cuts at: 5215 5463 6485 7464 5215
Size: 248 1022 979 5532
Fragments arranged by size:
5532 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 443 475 1760 1786 1792 1818 2305 2337 3635
Size: 32 1285 26 6 26 487 32 1298
Cuts at: 3635 3667 4300 4332 4356 4388 4700 4732 5623
Size: 32 633 32 24 32 312 32 891
Cuts at: 5623 5653 5655 5685 443
Size: 30 2 30 2539
Fragments arranged by size:
2539 1298 1285 891 633 487 312 32
32 32 32 32 32 30 30 26
26 24 6 2
HincII GTy'rAC
Cuts at: 567 3694 4820 567
Size: 3127 1126 3528
Fragments arranged by size:
3528 3127 1126
HindIII A'AGCT_T
Cuts at: 781 4702 781
Size: 3921 3860
HpaI GTT'AAC
Cuts at: 567 567
Size: 7781
KasI G'GCGC_C
Cuts at: 5211 5459 5211
Size: 248 7533
KpnI G_GTAC'C
Cuts at: 4801 4801
Size: 7781
MfeI C'AATT_G
Cuts at: 659 659
Size: 7781
MluI A'CGCG_T
Cuts at: 7214 7214
Size: 7781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 3433 6452 6636 2738
Size: 695 3019 184 3883
Fragments arranged by size:
3883 3019 695 184
MscI TGG'CCA
Cuts at: 1698 3965 5542 1698
Size: 2267 1577 3937
Fragments arranged by size:
3937 2267 1577
MslI CAynn'nnrTG
Cuts at: 218 542 1918 4124 5770 5897 218
Size: 324 1376 2206 1646 127 2102
Fragments arranged by size:
2206 2102 1646 1376 324 127
MspA1I CmG'CkG
Cuts at: 114 154 1690 2098 2751 4460 4892 5206 5566
Size: 40 1536 408 653 1709 432 314 360
Cuts at: 5566 6579 6824 114
Size: 1013 245 1071
Fragments arranged by size:
1709 1536 1071 1013 653 432 408 360
314 245 40
NaeI GCC'GGC
Cuts at: 5962 5962
Size: 7781
NarI GG'CG_CC
Cuts at: 5212 5460 5212
Size: 248 7533
NdeI CA'TA_TG
Cuts at: 438 2997 438
Size: 2559 5222
NgoMIV G'CCGG_C
Cuts at: 5960 5960
Size: 7781
NheI G'CTAG_C
Cuts at: 2904 2904
Size: 7781
NruI TCG'CGA
Cuts at: 2528 2528
Size: 7781
NsiI A_TGCA'T
Cuts at: 1419 2997 1419
Size: 1578 6203
NspI r_CATG'y
Cuts at: 565 2538 2616 4834 5334 6241 565
Size: 1973 78 2218 500 907 2105
Fragments arranged by size:
2218 2105 1973 907 500 78
PciI A'CATG_T
Cuts at: 561 6237 561
Size: 5676 2105
PflMI CCAn_nnn'nTGG
Cuts at: 814 814
Size: 7781
PfoI T'CCnGG_A
Cuts at: 2051 2568 2051
Size: 517 7264
PmeI GTTT'AAAC
Cuts at: 4780 4780
Size: 7781
PmlI CAC'GTG
Cuts at: 2734 2734
Size: 7781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6920 6952 6920
Size: 32 7749
PpuMI rG'GwC_Cy
Cuts at: 4494 4494
Size: 7781
PshAI GACnn'nnGTC
Cuts at: 2726 2726
Size: 7781
PsiI TTA'TAA
Cuts at: 3434 3434
Size: 7781
PstI C_TGCA'G
Cuts at: 1674 1720 4828 5513 1674
Size: 46 3108 685 3942
Fragments arranged by size:
3942 3108 685 46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7781
PvuII CAG'CTG
Cuts at: 1690 2098 2751 4460 5206 5566 1690
Size: 408 653 1709 746 360 3905
Fragments arranged by size:
3905 1709 746 653 408 360
RsrII CG'GwC_CG
Cuts at: 5976 5976
Size: 7781
SacI G_AGCT'C
Cuts at: 2474 4795 2474
Size: 2321 5460
SacII CC_GC'GG
Cuts at: 155 155
Size: 7781
SalI G'TCGA_C
Cuts at: 3692 4818 3692
Size: 1126 6655
SapI GCTCTTCn'nnn_
Cuts at: 3951 5804 6014 3951
Size: 1853 210 5718
Fragments arranged by size:
5718 1853 210
SbfI CC_TGCA'GG
Cuts at: 4828 4828
Size: 7781
ScaI AGT'ACT
Cuts at: 3291 7298 3291
Size: 4007 3774
SfcI C'TryA_G
Cuts at: 33 1670 1716 1850 4076 4824 5509 6502 6693
Size: 1637 46 134 2226 748 685 993 191
Cuts at: 6693 33
Size: 1121
Fragments arranged by size:
2226 1637 1121 993 748 685 191 134
46
SfoI GGC'GCC
Cuts at: 5213 5461 5213
Size: 248 7533
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7781
SmaI CCC'GGG
Cuts at: 4803 4803
Size: 7781
SmlI C'TyrA_G
Cuts at: 310 1327 2131 2657 3652 3863 3954 4936 5851
Size: 1017 804 526 995 211 91 982 915
Cuts at: 5851 6343 6605 310
Size: 492 262 1486
Fragments arranged by size:
1486 1017 995 982 915 804 526 492
262 211 91
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7781
SphI G_CATG'C
Cuts at: 4834 5334 4834
Size: 500 7281
SspI AAT'ATT
Cuts at: 4284 4284
Size: 7781
StyI C'CwwG_G
Cuts at: 2519 2724 3081 3512 3740 4915 5269 2519
Size: 205 357 431 228 1175 354 5031
Fragments arranged by size:
5031 1175 431 357 354 228 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 915 4029 4737 5364 5497 5956 6165 915
Size: 3114 708 627 133 459 209 2531
Fragments arranged by size:
3114 2531 708 627 459 209 133
TatI w'GTAC_w
Cuts at: 2649 3289 3310 7296 2649
Size: 640 21 3986 3134
Fragments arranged by size:
3986 3134 640 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 477 509 7298 7330 477
Size: 32 6789 32 928
Fragments arranged by size:
6789 928 32 32
Tth111I GACn'n_nGTC
Cuts at: 5578 5578
Size: 7781
XbaI T'CTAG_A
Cuts at: 2836 3709 3724 4812 2836
Size: 873 15 1088 5805
Fragments arranged by size:
5805 1088 873 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1966 5276 1966
Size: 3310 4471
XmaI C'CCGG_G
Cuts at: 4801 4801
Size: 7781
XmnI GAAnn'nnTTC
Cuts at: 2985 4380 2985
Size: 1395 6386
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BglI BsiWI BsmBI BssHII BstEII BstZ17I CspCI EcoNI
FseI FspAI NcoI NotI PacI PasI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI