Restriction map for
pF1KB4092
(Circular) (Six-base) MAPSORT of: pF1KB4092.seq Check: 8680 from: 1 to: 4358
pF1KB4092 4358 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 220 220
Size: 4358
AccI GT'mk_AC
Cuts at: 1396 1396
Size: 4358
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4358
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 2183 2615 2968 3630 724
Size: 1459 432 353 662 1452
Fragments arranged by size:
1459 1452 662 432 353
AflIII A'CryG_T
Cuts at: 1710 3510 3791 1710
Size: 1800 281 2277
Fragments arranged by size:
2277 1800 281
AgeI A'CCGG_T
Cuts at: 347 347
Size: 4358
AlwNI CAG_nnn'CTG
Cuts at: 905 1663 3101 905
Size: 758 1438 2162
Fragments arranged by size:
2162 1438 758
ApaI G_GGCC'C
Cuts at: 121 734 121
Size: 613 3745
ApaLI G'TGCA_C
Cuts at: 3196 3196
Size: 4358
ApoI r'AATT_y
Cuts at: 1191 1362 3711 3967 1191
Size: 171 2349 256 1582
Fragments arranged by size:
2349 1582 256 171
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 745 1378 745
Size: 633 3725
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 808 1374 1788 2036 2071 808
Size: 566 414 248 35 3095
Fragments arranged by size:
3095 566 414 248 35
BanII G_rGCy'C
Cuts at: 121 734 1372 2402 121
Size: 613 638 1030 2077
Fragments arranged by size:
2077 1030 638 613
BbeI G_GCGC'C
Cuts at: 1792 2040 1792
Size: 248 4110
BbvCI CC'TCA_GC
Cuts at: 898 898
Size: 4358
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3312 4358 2256
Size: 1056 1046 2256
Fragments arranged by size:
2256 1056 1046
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 1470 1470
Size: 4358
Bme1580I G_kGCm'C
Cuts at: 121 641 734 1983 2076 3200 121
Size: 520 93 1249 93 1124 1279
Fragments arranged by size:
1279 1249 1124 520 93 93
BmgBI CAC'GTC
Cuts at: 186 186
Size: 4358
BmrI ACTGGGnnnn_n'
Cuts at: 1570 1651 1986 4130 1570
Size: 81 335 2144 1798
Fragments arranged by size:
2144 1798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 1303
Size: 4358
Bpu10I CC'TnA_GC
Cuts at: 898 898
Size: 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 3127 3425 1534
Size: 879 714 298 2467
Fragments arranged by size:
2467 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 887 4062 887
Size: 3175 1183
BsaAI yAC'GTr
Cuts at: 1711 1711
Size: 4358
BsaBI GATnn'nnATC
Cuts at: 6 1872 3909 6
Size: 1866 2037 455
Fragments arranged by size:
2037 1866 455
BsaHI Gr'CG_yC
Cuts at: 1789 2037 2739 4235 1789
Size: 248 702 1496 1912
Fragments arranged by size:
1912 1496 702 248
BsaWI w'CCGG_w
Cuts at: 347 913 1547 2068 2746 3157 3304 3578 347
Size: 566 634 521 678 411 147 274 1127
Fragments arranged by size:
1127 678 634 566 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 895 925 1276 1306 895
Size: 30 351 30 3947
Fragments arranged by size:
3947 351 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 853 1028 853
Size: 175 4183
BseYI C'CCAG_C
Cuts at: 114 1783 2541 3206 3614 114
Size: 1669 758 665 408 858
Fragments arranged by size:
1669 858 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 208 598 739 976 192
Size: 16 390 141 237 3574
Fragments arranged by size:
3574 390 237 141 16
BsiEI CG_ry'CG
Cuts at: 104 1010 1900 1946 3176 104
Size: 906 890 46 1230 1286
Fragments arranged by size:
1286 1230 906 890 46
BsiHKAI G_wGCw'C
Cuts at: 964 1372 2150 2340 3200 964
Size: 408 778 190 860 2122
Fragments arranged by size:
2122 860 778 408 190
Bsp1286I G_dGCh'C
Cuts at: 121 641 734 964 1372 1983 2076 2150 2340
Size: 520 93 230 408 611 93 74 190
Cuts at: 2340 2402 3200 121
Size: 62 798 1279
Fragments arranged by size:
1279 798 611 520 408 230 190 93
93 74 62
BspEI T'CCGG_A
Cuts at: 913 913
Size: 4358
BspHI T'CATG_A
Cuts at: 216 1119 2790 216
Size: 903 1671 1784
Fragments arranged by size:
1784 1671 903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 1408 1622 1924 2305 220
Size: 1188 214 302 381 2273
Fragments arranged by size:
2273 1188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2650 2704 4188 4353 50
Size: 2600 54 1484 165 55
Fragments arranged by size:
2600 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 532 1218 2270 532
Size: 686 1052 2620
Fragments arranged by size:
2620 1052 686
BsrFI r'CCGG_y
Cuts at: 347 740 1315 2356 2537 347
Size: 393 575 1041 181 2168
Fragments arranged by size:
2168 1041 575 393 181
BssSI C'ACGA_G
Cuts at: 2629 3337 2629
Size: 708 3650
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1735
Size: 4358
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4358
BstYI r'GATC_y
Cuts at: 1 693 1383 1850 1873 2208 2454 2760 2772
Size: 692 690 467 23 335 246 306 12
Cuts at: 2772 2858 3910 1
Size: 86 1052 449
Fragments arranged by size:
1052 692 690 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 122 122
Size: 4358
BtgI C'CryG_G
Cuts at: 153 153
Size: 4358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2308 2489 2652 2308
Size: 181 163 4014
Fragments arranged by size:
4014 181 163
BtsI GCAGTG_nn'
Cuts at: 1601 1601
Size: 4358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 135 170 135
Size: 35 4323
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DrdI GACnn_nn'nnGTC
Cuts at: 2064 3408 2064
Size: 1344 3014
EaeI y'GGCC_r
Cuts at: 724 1943 2117 2508 2535 3880 724
Size: 1219 174 391 27 1345 1202
Fragments arranged by size:
1345 1219 1202 391 174 27
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4358
EarI CTCTTCn'nnn_
Cuts at: 1077 1189 2381 2591 1077
Size: 112 1192 210 2844
Fragments arranged by size:
2844 1192 210 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2566 3308 3454 4168 2566
Size: 742 146 714 2756
Fragments arranged by size:
2756 742 714 146
EcoICRI GAG'CTC
Cuts at: 1370 1370
Size: 4358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1303 2183 2615 2968 3630 724
Size: 579 880 432 353 662 1452
Fragments arranged by size:
1452 880 662 579 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 125 125
Size: 4358
EcoO109I rG'GnC_Cy
Cuts at: 118 730 731 990 1497 118
Size: 612 1 259 507 2979
Fragments arranged by size:
2979 612 507 259 1
EcoRI G'AATT_C
Cuts at: 1191 1362 1191
Size: 171 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4358
HaeII r_GCGC'y
Cuts at: 431 1792 2040 3270 4041 431
Size: 1361 248 1230 771 748
Fragments arranged by size:
1361 1230 771 748 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 2200 2230 2232 2262 1276
Size: 32 892 30 2 30 3372
Fragments arranged by size:
3372 892 32 30 30 2
HincII GTy'rAC
Cuts at: 1397 1397
Size: 4358
KasI G'GCGC_C
Cuts at: 1788 2036 1788
Size: 248 4110
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4358
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 3117 3301 488
Size: 2629 184 1545
Fragments arranged by size:
2629 1545 184
MscI TGG'CCA
Cuts at: 2119 2119
Size: 4358
MslI CAynn'nnrTG
Cuts at: 358 468 652 715 1249 2347 2474 358
Size: 110 184 63 534 1098 127 2242
Fragments arranged by size:
2242 1098 534 184 127 110 63
MspA1I CmG'CkG
Cuts at: 723 1469 1783 2143 2927 3172 723
Size: 746 314 360 784 245 1909
Fragments arranged by size:
1909 784 746 360 314 245
NaeI GCC'GGC
Cuts at: 1317 2539 1317
Size: 1222 3136
NarI GG'CG_CC
Cuts at: 1789 2037 1789
Size: 248 4110
NgoMIV G'CCGG_C
Cuts at: 1315 2537 1315
Size: 1222 3136
NspI r_CATG'y
Cuts at: 1411 1911 3514 1411
Size: 500 1603 2255
Fragments arranged by size:
2255 1603 500
PciI A'CATG_T
Cuts at: 3510 3510
Size: 4358
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 184 216 2804 2836 184
Size: 32 2588 32 1706
Fragments arranged by size:
2588 1706 32 32
PspOMI G'GGCC_C
Cuts at: 117 730 117
Size: 613 3745
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1006 1038 1006
Size: 32 4326
PstI C_TGCA'G
Cuts at: 1405 2090 1405
Size: 685 3673
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 1783 2143 1783
Size: 360 3998
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4358
SacI G_AGCT'C
Cuts at: 1372 1372
Size: 4358
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 2381 2591 2381
Size: 210 4148
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SfcI C'TryA_G
Cuts at: 33 813 1320 1401 2086 3054 3245 33
Size: 780 507 81 685 968 191 1146
Fragments arranged by size:
1146 968 780 685 507 191 81
SfoI GGC'GCC
Cuts at: 1790 2038 1790
Size: 248 4110
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 747 1380 747
Size: 633 3725
SmlI C'TyrA_G
Cuts at: 1513 2428 3142 3404 1513
Size: 915 714 262 2467
Fragments arranged by size:
2467 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
StuI AGG'CCT
Cuts at: 788 804 788
Size: 16 4342
StyI C'CwwG_G
Cuts at: 1492 1846 1492
Size: 354 4004
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 1024 1330 1941 2074 2533 2742 381
Size: 643 306 611 133 459 209 1997
Fragments arranged by size:
1997 643 611 459 306 209 133
TatI w'GTAC_w
Cuts at: 842 3873 842
Size: 3031 1327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4326
Tth111I GACn'n_nGTC
Cuts at: 2155 2155
Size: 4358
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1066 1297 1853 1066
Size: 231 556 3571
Fragments arranged by size:
3571 556 231
XmaI C'CCGG_G
Cuts at: 745 1378 745
Size: 633 3725
XmnI GAAnn'nnTTC
Cuts at: 488 488
Size: 4358
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI