Restriction map for
pF1KB4095
(Circular) (Six-base) MAPSORT of: pF1KB4095.seq Check: 9485 from: 1 to: 6362
pF1KB4095 6362 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2320 2320
Size: 6362
AccI GT'mk_AC
Cuts at: 185 3400 185
Size: 3215 3147
Acc65I G'GTAC_C
Cuts at: 3378 3378
Size: 6362
AclI AA'CG_TT
Cuts at: 6202 6202
Size: 6362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1648 3163 4187 4619 4972 5634 1030
Size: 618 1515 1024 432 353 662 1758
Fragments arranged by size:
1758 1515 1024 662 618 432 353
AflIII A'CryG_T
Cuts at: 1472 1781 3714 5514 5795 1472
Size: 309 1933 1800 281 2039
Fragments arranged by size:
2039 1933 1800 309 281
AhdI GACnn_n'nnGTC
Cuts at: 2691 2691
Size: 6362
AleI CACnn'nnGTG
Cuts at: 2120 2350 2120
Size: 230 6132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2128 2138 2128
Size: 10 6352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 988 1020 2646 2678 988
Size: 32 1626 32 4672
Fragments arranged by size:
4672 1626 32 32
AlwNI CAG_nnn'CTG
Cuts at: 603 1110 1776 3029 3667 5105 603
Size: 507 666 1253 638 1438 1860
Fragments arranged by size:
1860 1438 1253 666 638 507
ApaI G_GGCC'C
Cuts at: 2243 2243
Size: 6362
ApaLI G'TGCA_C
Cuts at: 5200 5200
Size: 6362
ApoI r'AATT_y
Cuts at: 1587 3366 5715 5971 1587
Size: 1779 2349 256 1978
Fragments arranged by size:
2349 1978 1779 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6362
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
AvaI C'yCGr_G
Cuts at: 1640 2367 2928 3382 1640
Size: 727 561 454 4620
Fragments arranged by size:
4620 727 561 454
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3068 3101 3068
Size: 33 6329
BamHI G'GATC_C
Cuts at: 621 1722 3387 621
Size: 1101 1665 3596
Fragments arranged by size:
3596 1665 1101
BanI G'GyrC_C
Cuts at: 744 858 2363 2838 3378 3792 4040 4075 744
Size: 114 1505 475 540 414 248 35 3031
Fragments arranged by size:
3031 1505 540 475 414 248 114 35
BanII G_rGCy'C
Cuts at: 462 578 989 1394 2041 2243 2359 2627 2935
Size: 116 411 405 647 202 116 268 308
Cuts at: 2935 2967 3376 4406 462
Size: 32 409 1030 2418
Fragments arranged by size:
2418 1030 647 411 409 405 308 268
202 116 116 32
BbeI G_GCGC'C
Cuts at: 2367 2842 3796 4044 2367
Size: 475 954 248 4685
Fragments arranged by size:
4685 954 475 248
BbvCI CC'TCA_GC
Cuts at: 1417 2591 1417
Size: 1174 5188
BciVI GTATCCnnnnn_n'
Cuts at: 3069 4260 5316 6362 3069
Size: 1191 1056 1046 3069
Fragments arranged by size:
3069 1191 1056 1046
BclI T'GATC_A
Cuts at: 1238 3882 1238
Size: 2644 3718
BfrBI ATG'CAT
Cuts at: 1620 1620
Size: 6362
BglI GCCn_nnn'nGGC
Cuts at: 326 1202 2159 2372 2852 2914 2939 326
Size: 876 957 213 480 62 25 3749
Fragments arranged by size:
3749 957 876 480 213 62 25
BglII A'GATC_T
Cuts at: 3877 5914 3877
Size: 2037 4325
BlpI GC'TnA_GC
Cuts at: 3474 3474
Size: 6362
Bme1580I G_kGCm'C
Cuts at: 863 2243 3987 4080 5204 863
Size: 1380 1744 93 1124 2021
Fragments arranged by size:
2021 1744 1380 1124 93
BmgBI CAC'GTC
Cuts at: 882 882
Size: 6362
BmrI ACTGGGnnnn_n'
Cuts at: 514 2114 3039 3574 3655 3990 6134 514
Size: 1600 925 535 81 335 2144 742
Fragments arranged by size:
2144 1600 925 742 535 335 81
BmtI G_CTAG'C
Cuts at: 2921 2921
Size: 6362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 432 2322 3036 4729 432
Size: 1890 714 1693 2065
Fragments arranged by size:
2065 1890 1693 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 1330 2147 2458 2981 3006 3205 501
Size: 829 817 311 523 25 199 3658
Fragments arranged by size:
3658 829 817 523 311 199 25
Bpu10I CC'TnA_GC
Cuts at: 968 1417 2591 3003 968
Size: 449 1174 412 4327
Fragments arranged by size:
4327 1174 449 412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1450 3298 3538 4417 5131 5429 922
Size: 528 1848 240 879 714 298 1855
Fragments arranged by size:
1855 1848 879 714 528 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 1756 6066 1756
Size: 4310 2052
BsaAI yAC'GTr
Cuts at: 774 798 981 1527 3715 774
Size: 24 183 546 2188 3421
Fragments arranged by size:
3421 2188 546 183 24
BsaBI GATnn'nnATC
Cuts at: 6 446 626 1441 3876 5913 6
Size: 440 180 815 2435 2037 455
Fragments arranged by size:
2435 2037 815 455 440 180
BsaHI Gr'CG_yC
Cuts at: 2364 2398 2419 2512 2728 2737 2839 3254 3793
Size: 34 21 93 216 9 102 415 539
Cuts at: 3793 4041 4743 6239 2364
Size: 248 702 1496 2487
Fragments arranged by size:
2487 1496 702 539 415 248 216 102
93 34 21 9
BsaWI w'CCGG_w
Cuts at: 3551 4072 4750 5161 5308 5582 3551
Size: 521 678 411 147 274 4331
Fragments arranged by size:
4331 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 539 569 988 1018 539
Size: 30 419 30 5883
Fragments arranged by size:
5883 419 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 157 187 259 262 271 274 295 1597
Size: 3 30 72 3 9 3 21 1302
Cuts at: 1597 154
Size: 4919
Fragments arranged by size:
4919 1302 72 30 21 9 3 3
3
BseYI C'CCAG_C
Cuts at: 593 988 1485 2032 2806 2812 2847 2874 2923
Size: 395 497 547 774 6 35 27 49
Cuts at: 2923 3787 4545 5210 5618 593
Size: 864 758 665 408 1337
Fragments arranged by size:
1337 864 774 758 665 547 497 408
395 49 35 27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1735 2287 224
Size: 1511 552 4299
Fragments arranged by size:
4299 1511 552
BsiEI CG_ry'CG
Cuts at: 104 1038 3904 3950 5180 104
Size: 934 2866 46 1230 1286
Fragments arranged by size:
2866 1286 1230 934 46
BsiHKAI G_wGCw'C
Cuts at: 578 887 1330 1349 1394 1637 2967 3133 3182
Size: 309 443 19 45 243 1330 166 49
Cuts at: 3182 3376 4154 4344 5204 578
Size: 194 778 190 860 1736
Fragments arranged by size:
1736 1330 860 778 443 309 243 194
190 166 49 45 19
BsmBI CGTCTCn'nnnn_
Cuts at: 544 544
Size: 6362
Bsp1286I G_dGCh'C
Cuts at: 462 578 863 887 989 1330 1349 1394 1637
Size: 116 285 24 102 341 19 45 243
Cuts at: 1637 2041 2243 2359 2627 2935 2967 3133 3182
Size: 404 202 116 268 308 32 166 49
Cuts at: 3182 3376 3987 4080 4154 4344 4406 5204 462
Size: 194 611 93 74 190 62 798 1620
Fragments arranged by size:
1620 798 611 404 341 308 285 268
243 202 194 190 166 116 116 102
93 74 62 49 45 32 24 19
BspHI T'CATG_A
Cuts at: 4794 4794
Size: 6362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 836 1409 1655 2320 3412 3626 3928 4309 836
Size: 573 246 665 1092 214 302 381 2889
Fragments arranged by size:
2889 1092 665 573 381 302 246 214
BsrBI CCG'CTC
Cuts at: 50 195 1341 4654 4708 6192 6357 50
Size: 145 1146 3313 54 1484 165 55
Fragments arranged by size:
3313 1484 1146 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 695 962 1968 4274 695
Size: 267 1006 2306 2783
Fragments arranged by size:
2783 2306 1006 267
BsrFI r'CCGG_y
Cuts at: 2739 4360 4541 2739
Size: 1621 181 4560
Fragments arranged by size:
4560 1621 181
BsrGI T'GTAC_A
Cuts at: 2747 2747
Size: 6362
BssSI C'ACGA_G
Cuts at: 4633 5341 4633
Size: 708 5654
BstAPI GCAn_nnn'nTGC
Cuts at: 603 858 3739 603
Size: 255 2881 3226
Fragments arranged by size:
3226 2881 255
BstBI TT'CG_AA
Cuts at: 4723 4723
Size: 6362
BstEII G'GTnAC_C
Cuts at: 3033 3033
Size: 6362
BstXI CCAn_nnnn'nTGG
Cuts at: 926 2328 2674 926
Size: 1402 346 4614
Fragments arranged by size:
4614 1402 346
BstYI r'GATC_y
Cuts at: 1 565 621 1412 1722 3317 3387 3854 3877
Size: 564 56 791 310 1595 70 467 23
Cuts at: 3877 4212 4458 4764 4776 4862 5914 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1595 1052 791 564 467 449 335 310
306 246 86 70 56 23 12
Bsu36I CC'TnA_GG
Cuts at: 233 233
Size: 6362
BtgI C'CryG_G
Cuts at: 321 668 2069 2285 2348 2903 3248 321
Size: 347 1401 216 63 555 345 3435
Fragments arranged by size:
3435 1401 555 347 345 216 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4312 4493 4656 4312
Size: 181 163 6018
Fragments arranged by size:
6018 181 163
BtsI GCAGTG_nn'
Cuts at: 168 219 2172 3117 3605 168
Size: 51 1953 945 488 2925
Fragments arranged by size:
2925 1953 945 488 51
ClaI AT'CG_AT
Cuts at: 425 425
Size: 6362
DraI TTT'AAA
Cuts at: 3361 3361
Size: 6362
DraIII CAC_nnn'GTG
Cuts at: 2025 2835 2025
Size: 810 5552
DrdI GACnn_nn'nnGTC
Cuts at: 4068 5412 4068
Size: 1344 5018
EaeI y'GGCC_r
Cuts at: 671 905 1542 2214 2741 3947 4121 4512 4539
Size: 234 637 672 527 1206 174 391 27
Cuts at: 4539 5884 671
Size: 1345 1149
Fragments arranged by size:
1345 1206 1149 672 637 527 391 234
174 27
EagI C'GGCC_G
Cuts at: 3947 3947
Size: 6362
EarI CTCTTCn'nnn_
Cuts at: 185 221 236 248 272 1951 4385 4595 185
Size: 36 15 12 24 1679 2434 210 1952
Fragments arranged by size:
2434 1952 1679 210 36 24 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 213 922 4570 5312 5458 6172 213
Size: 709 3648 742 146 714 403
Fragments arranged by size:
3648 742 714 709 403 146
EcoICRI GAG'CTC
Cuts at: 576 1392 2965 3374 576
Size: 816 1573 409 3564
Fragments arranged by size:
3564 1573 816 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 1030 1330 1648 2147 2458 2981 3006 3163
Size: 529 300 318 499 311 523 25 157
Cuts at: 3163 3205 4187 4619 4972 5634 501
Size: 42 982 432 353 662 1229
Fragments arranged by size:
1229 982 662 529 523 499 432 353
318 311 300 157 42 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2391 3232 2391
Size: 841 5521
EcoO109I rG'GnC_Cy
Cuts at: 1133 1461 1644 2239 2240 2552 2559 3501 1133
Size: 328 183 595 1 312 7 942 3994
Fragments arranged by size:
3994 942 595 328 312 183 7 1
EcoRI G'AATT_C
Cuts at: 3366 3366
Size: 6362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4381 4381
Size: 6362
FspI TGC'GCA
Cuts at: 688 2227 4143 688
Size: 1539 1916 2907
Fragments arranged by size:
2907 1916 1539
FspAI rTGC'GCAy
Cuts at: 2227 2227
Size: 6362
HaeII r_GCGC'y
Cuts at: 2367 2842 3796 4044 5274 6045 2367
Size: 475 954 248 1230 771 2684
Fragments arranged by size:
2684 1230 954 771 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 1138 1170 1654 1686 1926 1958 2677
Size: 32 544 32 484 32 240 32 719
Cuts at: 2677 2709 2749 2781 4204 4234 4236 4266 562
Size: 32 40 32 1423 30 2 30 2658
Fragments arranged by size:
2658 1423 719 544 484 240 40 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 354 2706 3401 354
Size: 2352 695 3315
Fragments arranged by size:
3315 2352 695
HindIII A'AGCT_T
Cuts at: 1953 1953
Size: 6362
KasI G'GCGC_C
Cuts at: 2363 2838 3792 4040 2363
Size: 475 954 248 4685
Fragments arranged by size:
4685 954 475 248
KpnI G_GTAC'C
Cuts at: 3382 3382
Size: 6362
MluI A'CGCG_T
Cuts at: 5795 5795
Size: 6362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 1380 1457 5121 5305 92
Size: 1288 77 3664 184 1149
Fragments arranged by size:
3664 1288 1149 184 77
MscI TGG'CCA
Cuts at: 907 4123 907
Size: 3216 3146
MslI CAynn'nnrTG
Cuts at: 772 1444 1879 2120 2260 2326 2350 3049 4351
Size: 672 435 241 140 66 24 699 1302
Cuts at: 4351 4478 772
Size: 127 2656
Fragments arranged by size:
2656 1302 699 672 435 241 140 127
66 24
MspA1I CmG'CkG
Cuts at: 208 603 1719 2340 3473 3787 4147 4931 5176
Size: 395 1116 621 1133 314 360 784 245
Cuts at: 5176 208
Size: 1394
Fragments arranged by size:
1394 1133 1116 784 621 395 360 314
245
NaeI GCC'GGC
Cuts at: 2741 4543 2741
Size: 1802 4560
NarI GG'CG_CC
Cuts at: 2364 2839 3793 4041 2364
Size: 475 954 248 4685
Fragments arranged by size:
4685 954 475 248
NcoI C'CATG_G
Cuts at: 321 2903 321
Size: 2582 3780
NgoMIV G'CCGG_C
Cuts at: 2739 4541 2739
Size: 1802 4560
NheI G'CTAG_C
Cuts at: 2917 2917
Size: 6362
NsiI A_TGCA'T
Cuts at: 1622 1622
Size: 6362
NspI r_CATG'y
Cuts at: 757 1476 1785 2001 3129 3415 3915 5518 757
Size: 719 309 216 1128 286 500 1603 1601
Fragments arranged by size:
1603 1601 1128 719 500 309 286 216
PasI CC'CwG_GG
Cuts at: 1137 2235 1137
Size: 1098 5264
PciI A'CATG_T
Cuts at: 1472 1781 5514 1472
Size: 309 3733 2320
Fragments arranged by size:
3733 2320 309
PflMI CCAn_nnn'nTGG
Cuts at: 632 855 1734 2835 3029 632
Size: 223 879 1101 194 3965
Fragments arranged by size:
3965 1101 879 223 194
PfoI T'CCnGG_A
Cuts at: 437 2474 3347 437
Size: 2037 873 3452
Fragments arranged by size:
3452 2037 873
PmeI GTTT'AAAC
Cuts at: 3361 3361
Size: 6362
PmlI CAC'GTG
Cuts at: 798 1527 798
Size: 729 5633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 4808 4840 988
Size: 32 3788 32 2510
Fragments arranged by size:
3788 2510 32 32
PpuMI rG'GwC_Cy
Cuts at: 1133 1461 1644 2559 1133
Size: 328 183 915 4936
Fragments arranged by size:
4936 915 328 183
PshAI GACnn'nnGTC
Cuts at: 3100 3100
Size: 6362
PspOMI G'GGCC_C
Cuts at: 2239 2239
Size: 6362
PstI C_TGCA'G
Cuts at: 728 1406 1652 3409 4094 728
Size: 678 246 1757 685 2996
Fragments arranged by size:
2996 1757 685 678 246
PvuI CG_AT'CG
Cuts at: 104 1038 104
Size: 934 5428
PvuII CAG'CTG
Cuts at: 603 1719 3787 4147 603
Size: 1116 2068 360 2818
Fragments arranged by size:
2818 2068 1116 360
RsrII CG'GwC_CG
Cuts at: 4557 4557
Size: 6362
SacI G_AGCT'C
Cuts at: 578 1394 2967 3376 578
Size: 816 1573 409 3564
Fragments arranged by size:
3564 1573 816 409
SalI G'TCGA_C
Cuts at: 3399 3399
Size: 6362
SanDI GG'GwC_CC
Cuts at: 2559 2559
Size: 6362
SapI GCTCTTCn'nnn_
Cuts at: 185 221 4385 4595 185
Size: 36 4164 210 1952
Fragments arranged by size:
4164 1952 210 36
SbfI CC_TGCA'GG
Cuts at: 1406 3409 1406
Size: 2003 4359
ScaI AGT'ACT
Cuts at: 5879 5879
Size: 6362
SexAI A'CCwGG_T
Cuts at: 2021 2831 2021
Size: 810 5552
SfcI C'TryA_G
Cuts at: 33 724 1402 1648 2943 3405 4090 5058 5249
Size: 691 678 246 1295 462 685 968 191
Cuts at: 5249 33
Size: 1146
Fragments arranged by size:
1295 1146 968 691 685 678 462 246
191
SfoI GGC'GCC
Cuts at: 2365 2840 3794 4042 2365
Size: 475 954 248 4685
Fragments arranged by size:
4685 954 475 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
SmaI CCC'GGG
Cuts at: 1642 2369 3384 1642
Size: 727 1015 4620
Fragments arranged by size:
4620 1015 727
SmlI C'TyrA_G
Cuts at: 937 1465 3313 3517 4432 5146 5408 937
Size: 528 1848 204 915 714 262 1891
Fragments arranged by size:
1891 1848 915 714 528 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6362
SphI G_CATG'C
Cuts at: 3415 3915 3415
Size: 500 5862
SrfI GCCC'GGGC
Cuts at: 2369 2369
Size: 6362
StuI AGG'CCT
Cuts at: 781 1565 2644 781
Size: 784 1079 4499
Fragments arranged by size:
4499 1079 784
StyI C'CwwG_G
Cuts at: 321 1853 2903 3496 3850 321
Size: 1532 1050 593 354 2833
Fragments arranged by size:
2833 1532 1050 593 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 314 607 1726 2062 2463 3945 4078 4537 4746
Size: 293 1119 336 401 1482 133 459 209
Cuts at: 4746 314
Size: 1930
Fragments arranged by size:
1930 1482 1119 459 401 336 293 209
133
TatI w'GTAC_w
Cuts at: 2747 5877 2747
Size: 3130 3232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 539 571 5879 5911 539
Size: 32 5308 32 990
Fragments arranged by size:
5308 990 32 32
Tth111I GACn'n_nGTC
Cuts at: 4159 4159
Size: 6362
XbaI T'CTAG_A
Cuts at: 405 3393 405
Size: 2988 3374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 385 540 816 2067 2292 3180 3857 385
Size: 155 276 1251 225 888 677 2890
Fragments arranged by size:
2890 1251 888 677 276 225 155
XmaI C'CCGG_G
Cuts at: 1640 2367 3382 1640
Size: 727 1015 4620
Fragments arranged by size:
4620 1015 727
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AscI AvrII BbsI BcgI
BsiWI BsmI BspEI BssHII BstZ17I CspCI EcoRV FseI
HpaI MfeI NdeI NotI NruI PacI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI