Restriction map for
pF1KB4102
(Circular) (Six-base) MAPSORT of: pF1KB4102.seq Check: 8107 from: 1 to: 5258
pF1KB4102 5258 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1787 1787
Size: 5258
AccI GT'mk_AC
Cuts at: 2296 2296
Size: 5258
Acc65I G'GTAC_C
Cuts at: 2274 2274
Size: 5258
AclI AA'CG_TT
Cuts at: 5098 5098
Size: 5258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 1385 3083 3515 3868 4530 419
Size: 966 1698 432 353 662 1147
Fragments arranged by size:
1698 1147 966 662 432 353
AflIII A'CryG_T
Cuts at: 125 1946 2610 4410 4691 125
Size: 1821 664 1800 281 692
Fragments arranged by size:
1821 1800 692 664 281
AleI CACnn'nnGTG
Cuts at: 847 1519 2181 847
Size: 672 662 3924
Fragments arranged by size:
3924 672 662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1210 1210
Size: 5258
AlwNI CAG_nnn'CTG
Cuts at: 282 393 889 937 997 1230 1363 1789 2563
Size: 111 496 48 60 233 133 426 774
Cuts at: 2563 4001 282
Size: 1438 1539
Fragments arranged by size:
1539 1438 774 496 426 233 133 111
60 48
ApaLI G'TGCA_C
Cuts at: 4096 4096
Size: 5258
ApoI r'AATT_y
Cuts at: 2224 2262 4611 4867 2224
Size: 38 2349 256 2615
Fragments arranged by size:
2615 2349 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 5258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
AvaI C'yCGr_G
Cuts at: 2278 2278
Size: 5258
BamHI G'GATC_C
Cuts at: 2283 2283
Size: 5258
BanI G'GyrC_C
Cuts at: 835 1122 1480 2274 2688 2936 2971 835
Size: 287 358 794 414 248 35 3122
Fragments arranged by size:
3122 794 414 358 287 248 35
BanII G_rGCy'C
Cuts at: 146 2272 3302 146
Size: 2126 1030 2102
Fragments arranged by size:
2126 2102 1030
BbeI G_GCGC'C
Cuts at: 1484 2692 2940 1484
Size: 1208 248 3802
Fragments arranged by size:
3802 1208 248
BbsI GAAGACnn'nnnn_
Cuts at: 1611 1611
Size: 5258
BbvCI CC'TCA_GC
Cuts at: 930 998 1183 930
Size: 68 185 5005
Fragments arranged by size:
5005 185 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1539 1573 1656 1690 2198 2232 1539
Size: 34 83 34 508 34 4565
Fragments arranged by size:
4565 508 83 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3156 4212 5258 3156
Size: 1056 1046 3156
Fragments arranged by size:
3156 1056 1046
BclI T'GATC_A
Cuts at: 2778 2778
Size: 5258
BfrBI ATG'CAT
Cuts at: 2079 2079
Size: 5258
BglI GCCn_nnn'nGGC
Cuts at: 223 278 322 670 1203 1420 223
Size: 55 44 348 533 217 4061
Fragments arranged by size:
4061 533 348 217 55 44
BglII A'GATC_T
Cuts at: 1838 2773 4810 1838
Size: 935 2037 2286
Fragments arranged by size:
2286 2037 935
BlpI GC'TnA_GC
Cuts at: 2370 2370
Size: 5258
Bme1580I G_kGCm'C
Cuts at: 827 1013 1293 1643 1669 2883 2976 4100 827
Size: 186 280 350 26 1214 93 1124 1985
Fragments arranged by size:
1985 1214 1124 350 280 186 93 26
BmgBI CAC'GTC
Cuts at: 1947 1947
Size: 5258
BmrI ACTGGGnnnn_n'
Cuts at: 548 1674 2175 2470 2551 2886 5030 548
Size: 1126 501 295 81 335 2144 776
Fragments arranged by size:
2144 1126 776 501 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2085 2190 3625 2085
Size: 105 1435 3718
Fragments arranged by size:
3718 1435 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 304 370 426 759 780 1252 283
Size: 21 66 56 333 21 472 4289
Fragments arranged by size:
4289 472 333 66 56 21 21
Bpu10I CC'TnA_GC
Cuts at: 930 998 1063 1183 1816 930
Size: 68 65 120 633 4372
Fragments arranged by size:
4372 633 120 68 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 1915 2434 3313 4027 4325 1453
Size: 462 519 879 714 298 2386
Fragments arranged by size:
2386 879 714 519 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 166 406 647 2164 4962 166
Size: 240 241 1517 2798 462
Fragments arranged by size:
2798 1517 462 241 240
BsaAI yAC'GTr
Cuts at: 249 2611 249
Size: 2362 2896
BsaBI GATnn'nnATC
Cuts at: 6 2772 4809 6
Size: 2766 2037 455
Fragments arranged by size:
2766 2037 455
BsaHI Gr'CG_yC
Cuts at: 1271 1481 2689 2937 3639 5135 1271
Size: 210 1208 248 702 1496 1394
Fragments arranged by size:
1496 1394 1208 702 248 210
BsaWI w'CCGG_w
Cuts at: 404 2447 2968 3646 4057 4204 4478 404
Size: 2043 521 678 411 147 274 1184
Fragments arranged by size:
2043 1184 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1622 1652 1622
Size: 30 5228
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 723 997 1147 1345 1456 2179 2182 723
Size: 274 150 198 111 723 3 3799
Fragments arranged by size:
3799 723 274 198 150 111 3
BseYI C'CCAG_C
Cuts at: 526 1274 2235 2248 2683 3441 4106 4514 526
Size: 748 961 13 435 758 665 408 1270
Fragments arranged by size:
1270 961 758 748 665 435 408 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1509 1759 1509
Size: 250 5008
BsiEI CG_ry'CG
Cuts at: 104 2800 2846 4076 104
Size: 2696 46 1230 1286
Fragments arranged by size:
2696 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1661 2272 3050 3240 4100 1661
Size: 611 778 190 860 2819
Fragments arranged by size:
2819 860 778 611 190
BsmBI CGTCTCn'nnnn_
Cuts at: 232 232
Size: 5258
Bsp1286I G_dGCh'C
Cuts at: 146 827 1013 1293 1643 1661 1669 2272 2883
Size: 681 186 280 350 18 8 603 611
Cuts at: 2883 2976 3050 3240 3302 4100 146
Size: 93 74 190 62 798 1304
Fragments arranged by size:
1304 798 681 611 603 350 280 190
186 93 74 62 18 8
BspHI T'CATG_A
Cuts at: 3690 3690
Size: 5258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 1140 1197 1763 1781 1787 2114 2141 2308
Size: 868 57 566 18 6 327 27 167
Cuts at: 2308 2522 2824 3205 272
Size: 214 302 381 2325
Fragments arranged by size:
2325 868 566 381 327 302 214 167
57 27 18 6
BsrBI CCG'CTC
Cuts at: 50 134 537 3550 3604 5088 5253 50
Size: 84 403 3013 54 1484 165 55
Fragments arranged by size:
3013 1484 403 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 3170 3170
Size: 5258
BsrFI r'CCGG_y
Cuts at: 1125 2120 3256 3437 1125
Size: 995 1136 181 2946
Fragments arranged by size:
2946 1136 995 181
BsrGI T'GTAC_A
Cuts at: 327 327
Size: 5258
BssHII G'CGCG_C
Cuts at: 116 200 226 116
Size: 84 26 5148
Fragments arranged by size:
5148 84 26
BssSI C'ACGA_G
Cuts at: 3529 4237 3529
Size: 708 4550
BstAPI GCAn_nnn'nTGC
Cuts at: 2635 2635
Size: 5258
BstBI TT'CG_AA
Cuts at: 3619 3619
Size: 5258
BstXI CCAn_nnnn'nTGG
Cuts at: 153 153
Size: 5258
BstYI r'GATC_y
Cuts at: 1 593 1838 2283 2750 2773 3108 3354 3660
Size: 592 1245 445 467 23 335 246 306
Cuts at: 3660 3672 3758 4810 1
Size: 12 86 1052 449
Fragments arranged by size:
1245 1052 592 467 449 445 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1200 1200
Size: 5258
BtgI C'CryG_G
Cuts at: 164 1613 1625 164
Size: 1449 12 3797
Fragments arranged by size:
3797 1449 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2010 3208 3389 3552 2010
Size: 1198 181 163 3716
Fragments arranged by size:
3716 1198 181 163
BtsI GCAGTG_nn'
Cuts at: 237 1450 1643 2501 237
Size: 1213 193 858 2994
Fragments arranged by size:
2994 1213 858 193
DraI TTT'AAA
Cuts at: 2257 2257
Size: 5258
DrdI GACnn_nn'nnGTC
Cuts at: 1798 2964 4308 1798
Size: 1166 1344 2748
Fragments arranged by size:
2748 1344 1166
EaeI y'GGCC_r
Cuts at: 215 687 1085 1127 1610 1961 2843 3017 3408
Size: 472 398 42 483 351 882 174 391
Cuts at: 3408 3435 4780 215
Size: 27 1345 693
Fragments arranged by size:
1345 882 693 483 472 398 391 351
174 42 27
EagI C'GGCC_G
Cuts at: 2843 2843
Size: 5258
EarI CTCTTCn'nnn_
Cuts at: 3281 3491 3281
Size: 210 5048
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 3466 4208 4354 5068 154
Size: 3312 742 146 714 344
Fragments arranged by size:
3312 742 714 344 146
EcoICRI GAG'CTC
Cuts at: 2270 2270
Size: 5258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 304 370 419 426 759 780 1252 1385
Size: 21 66 49 7 333 21 472 133
Cuts at: 1385 3083 3515 3868 4530 283
Size: 1698 432 353 662 1011
Fragments arranged by size:
1698 1011 662 472 432 353 333 133
66 49 21 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 286 2161 286
Size: 1875 3383
EcoO109I rG'GnC_Cy
Cuts at: 279 430 663 1152 1179 2397 279
Size: 151 233 489 27 1218 3140
Fragments arranged by size:
3140 1218 489 233 151 27
EcoRI G'AATT_C
Cuts at: 2224 2262 2224
Size: 38 5220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 5258
FspI TGC'GCA
Cuts at: 1891 2056 3039 1891
Size: 165 983 4110
Fragments arranged by size:
4110 983 165
FspAI rTGC'GCAy
Cuts at: 1891 1891
Size: 5258
HaeII r_GCGC'y
Cuts at: 651 1484 2692 2940 4170 4941 651
Size: 833 1208 248 1230 771 968
Fragments arranged by size:
1230 1208 968 833 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 1387 1419 3100 3130 3132 3162 403
Size: 32 952 32 1681 30 2 30 2499
Fragments arranged by size:
2499 1681 952 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2297 2297
Size: 5258
KasI G'GCGC_C
Cuts at: 1480 2688 2936 1480
Size: 1208 248 3802
Fragments arranged by size:
3802 1208 248
KpnI G_GTAC'C
Cuts at: 2278 2278
Size: 5258
MluI A'CGCG_T
Cuts at: 125 4691 125
Size: 4566 692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4017 4201 4017
Size: 184 5074
MscI TGG'CCA
Cuts at: 689 1087 1612 3019 689
Size: 398 525 1407 2928
Fragments arranged by size:
2928 1407 525 398
MslI CAynn'nnrTG
Cuts at: 340 485 847 1099 1519 1829 1990 2014 2181
Size: 145 362 252 420 310 161 24 167
Cuts at: 2181 3247 3374 340
Size: 1066 127 2224
Fragments arranged by size:
2224 1066 420 362 310 252 167 161
145 127 24
MspA1I CmG'CkG
Cuts at: 166 728 800 997 1319 1734 1786 1969 2369
Size: 562 72 197 322 415 52 183 400
Cuts at: 2369 2683 3043 3827 4072 166
Size: 314 360 784 245 1352
Fragments arranged by size:
1352 784 562 415 400 360 322 314
245 197 183 72 52
NaeI GCC'GGC
Cuts at: 1127 3439 1127
Size: 2312 2946
NarI GG'CG_CC
Cuts at: 1481 2689 2937 1481
Size: 1208 248 3802
Fragments arranged by size:
3802 1208 248
NcoI C'CATG_G
Cuts at: 1613 1625 1613
Size: 12 5246
NgoMIV G'CCGG_C
Cuts at: 1125 3437 1125
Size: 2312 2946
NsiI A_TGCA'T
Cuts at: 2081 2081
Size: 5258
NspI r_CATG'y
Cuts at: 1560 2083 2311 2811 4414 1560
Size: 523 228 500 1603 2404
Fragments arranged by size:
2404 1603 523 500 228
PasI CC'CwG_GG
Cuts at: 954 954
Size: 5258
PciI A'CATG_T
Cuts at: 4410 4410
Size: 5258
PflMI CCAn_nnn'nTGG
Cuts at: 483 483
Size: 5258
PmeI GTTT'AAAC
Cuts at: 2257 2257
Size: 5258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 5226
PpuMI rG'GwC_Cy
Cuts at: 1152 1179 1152
Size: 27 5231
PstI C_TGCA'G
Cuts at: 278 498 903 1943 2111 2138 2305 2990 278
Size: 220 405 1040 168 27 167 685 2546
Fragments arranged by size:
2546 1040 685 405 220 168 167 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5258
PvuII CAG'CTG
Cuts at: 800 997 1734 1786 1969 2683 3043 800
Size: 197 737 52 183 714 360 3015
Fragments arranged by size:
3015 737 714 360 197 183 52
RsrII CG'GwC_CG
Cuts at: 3453 3453
Size: 5258
SacI G_AGCT'C
Cuts at: 2272 2272
Size: 5258
SacII CC_GC'GG
Cuts at: 167 167
Size: 5258
SalI G'TCGA_C
Cuts at: 2295 2295
Size: 5258
SapI GCTCTTCn'nnn_
Cuts at: 3281 3491 3281
Size: 210 5048
SbfI CC_TGCA'GG
Cuts at: 278 1943 2138 2305 278
Size: 1665 195 167 3231
Fragments arranged by size:
3231 1665 195 167
ScaI AGT'ACT
Cuts at: 4775 4775
Size: 5258
SfcI C'TryA_G
Cuts at: 33 274 494 899 1538 1939 2084 2107 2134
Size: 241 220 405 639 401 145 23 27
Cuts at: 2134 2301 2986 3954 4145 33
Size: 167 685 968 191 1146
Fragments arranged by size:
1146 968 685 639 405 401 241 220
191 167 145 27 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 670 1420 670
Size: 750 4508
SfoI GGC'GCC
Cuts at: 1482 2690 2938 1482
Size: 1208 248 3802
Fragments arranged by size:
3802 1208 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
SmaI CCC'GGG
Cuts at: 2280 2280
Size: 5258
SmlI C'TyrA_G
Cuts at: 1468 1930 2413 3328 4042 4304 1468
Size: 462 483 915 714 262 2422
Fragments arranged by size:
2422 915 714 483 462 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5258
SphI G_CATG'C
Cuts at: 2083 2311 2811 2083
Size: 228 500 4530
Fragments arranged by size:
4530 500 228
StuI AGG'CCT
Cuts at: 584 1117 584
Size: 533 4725
StyI C'CwwG_G
Cuts at: 875 1415 1613 1625 1715 2392 2746 875
Size: 540 198 12 90 677 354 3387
Fragments arranged by size:
3387 677 540 354 198 90 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 636 822 853 2841 2974 3433 3642 636
Size: 186 31 1988 133 459 209 2252
Fragments arranged by size:
2252 1988 459 209 186 133 31
TatI w'GTAC_w
Cuts at: 327 359 4773 327
Size: 32 4414 812
Fragments arranged by size:
4414 812 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1622 1654 1910 1942 4775 4807 1622
Size: 32 256 32 2833 32 2073
Fragments arranged by size:
2833 2073 256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 417 3055 417
Size: 2638 2620
XbaI T'CTAG_A
Cuts at: 2289 2289
Size: 5258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1491 2753 1491
Size: 1262 3996
XmaI C'CCGG_G
Cuts at: 2278 2278
Size: 5258
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BmtI BsiWI BsmI BspEI BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI