Restriction map for
pF1KB4107
(Circular) (Six-base) MAPSORT of: pF1KB4107.seq Check: 4141 from: 1 to: 5036
pF1KB4107 5036 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1365 1365
Size: 5036
AccI GT'mk_AC
Cuts at: 2074 2074
Size: 5036
Acc65I G'GTAC_C
Cuts at: 2052 2052
Size: 5036
AclI AA'CG_TT
Cuts at: 4876 4876
Size: 5036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 2861 3293 3646 4308 1220
Size: 1641 432 353 662 1948
Fragments arranged by size:
1948 1641 662 432 353
AflIII A'CryG_T
Cuts at: 806 2388 4188 4469 806
Size: 1582 1800 281 1373
Fragments arranged by size:
1800 1582 1373 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 659 691 659
Size: 32 5004
AlwNI CAG_nnn'CTG
Cuts at: 2341 3779 2341
Size: 1438 3598
ApaI G_GGCC'C
Cuts at: 1466 1466
Size: 5036
ApaLI G'TGCA_C
Cuts at: 973 3874 973
Size: 2901 2135
ApoI r'AATT_y
Cuts at: 145 967 1159 2040 4389 4645 145
Size: 822 192 881 2349 256 536
Fragments arranged by size:
2349 881 822 536 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 5036
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
AvaI C'yCGr_G
Cuts at: 638 2056 638
Size: 1418 3618
BamHI G'GATC_C
Cuts at: 252 2061 252
Size: 1809 3227
BanI G'GyrC_C
Cuts at: 586 1641 2052 2466 2714 2749 586
Size: 1055 411 414 248 35 2873
Fragments arranged by size:
2873 1055 414 411 248 35
BanII G_rGCy'C
Cuts at: 465 1422 1466 2050 3080 465
Size: 957 44 584 1030 2421
Fragments arranged by size:
2421 1030 957 584 44
BbeI G_GCGC'C
Cuts at: 2470 2718 2470
Size: 248 4788
BbsI GAAGACnn'nnnn_
Cuts at: 809 809
Size: 5036
BbvCI CC'TCA_GC
Cuts at: 618 618
Size: 5036
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3990 5036 2934
Size: 1056 1046 2934
Fragments arranged by size:
2934 1056 1046
BclI T'GATC_A
Cuts at: 2556 2556
Size: 5036
BglI GCCn_nnn'nGGC
Cuts at: 1380 1380
Size: 5036
BglII A'GATC_T
Cuts at: 118 777 2551 4588 118
Size: 659 1774 2037 566
Fragments arranged by size:
2037 1774 659 566
BlpI GC'TnA_GC
Cuts at: 500 2148 500
Size: 1648 3388
Bme1580I G_kGCm'C
Cuts at: 591 977 1466 1716 1847 2661 2754 3878 591
Size: 386 489 250 131 814 93 1124 1749
Fragments arranged by size:
1749 1124 814 489 386 250 131 93
BmrI ACTGGGnnnn_n'
Cuts at: 301 2248 2329 2664 4808 301
Size: 1947 81 335 2144 529
Fragments arranged by size:
2144 1947 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3403 3403
Size: 5036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1397 1469 1517 1580 1397
Size: 72 48 63 4853
Fragments arranged by size:
4853 72 63 48
Bpu10I CC'TnA_GC
Cuts at: 618 618
Size: 5036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 2212 3091 3805 4103 719
Size: 1493 879 714 298 1652
Fragments arranged by size:
1652 1493 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1428 4740 1428
Size: 3312 1724
BsaAI yAC'GTr
Cuts at: 2389 2389
Size: 5036
BsaBI GATnn'nnATC
Cuts at: 6 123 878 2550 4587 6
Size: 117 755 1672 2037 455
Fragments arranged by size:
2037 1672 755 455 117
BsaHI Gr'CG_yC
Cuts at: 2467 2715 3417 4913 2467
Size: 248 702 1496 2590
Fragments arranged by size:
2590 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2225 2746 3424 3835 3982 4256 2225
Size: 521 678 411 147 274 3005
Fragments arranged by size:
3005 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1875 1905 1875
Size: 30 5006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 719 722 1082 1153 1412 1547 719
Size: 3 360 71 259 135 4208
Fragments arranged by size:
4208 360 259 135 71 3
BseYI C'CCAG_C
Cuts at: 590 830 2461 3219 3884 4292 590
Size: 240 1631 758 665 408 1334
Fragments arranged by size:
1631 1334 758 665 408 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1574 1778 1835 763
Size: 811 204 57 3964
Fragments arranged by size:
3964 811 204 57
BsiEI CG_ry'CG
Cuts at: 104 2578 2624 3854 104
Size: 2474 46 1230 1286
Fragments arranged by size:
2474 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 977 1422 2050 2828 3018 3878 977
Size: 445 628 778 190 860 2135
Fragments arranged by size:
2135 860 778 628 445 190
BsmI GAATG_Cn'
Cuts at: 501 1451 501
Size: 950 4086
Bsp1286I G_dGCh'C
Cuts at: 465 591 977 1422 1466 1716 1847 2050 2661
Size: 126 386 445 44 250 131 203 611
Cuts at: 2661 2754 2828 3018 3080 3878 465
Size: 93 74 190 62 798 1623
Fragments arranged by size:
1623 798 611 445 386 250 203 190
131 126 93 74 62 44
BspHI T'CATG_A
Cuts at: 840 2006 3468 840
Size: 1166 1462 2408
Fragments arranged by size:
2408 1462 1166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1365 1665 2086 2300 2602 2983 1111
Size: 254 300 421 214 302 381 3164
Fragments arranged by size:
3164 421 381 302 300 254 214
BsrBI CCG'CTC
Cuts at: 50 3328 3382 4866 5031 50
Size: 3278 54 1484 165 55
Fragments arranged by size:
3278 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2948 2948
Size: 5036
BsrFI r'CCGG_y
Cuts at: 3034 3215 3034
Size: 181 4855
BssSI C'ACGA_G
Cuts at: 1559 3307 4015 1559
Size: 1748 708 2580
Fragments arranged by size:
2580 1748 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2413 2413
Size: 5036
BstBI TT'CG_AA
Cuts at: 3397 3397
Size: 5036
BstXI CCAn_nnnn'nTGG
Cuts at: 1249 1249
Size: 5036
BstYI r'GATC_y
Cuts at: 1 118 252 777 2061 2528 2551 2886 3132
Size: 117 134 525 1284 467 23 335 246
Cuts at: 3132 3438 3450 3536 4588 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1284 1052 525 467 449 335 306 246
134 117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 884 1136 884
Size: 252 4784
BtgI C'CryG_G
Cuts at: 107 107
Size: 5036
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2986 3167 3330 2986
Size: 181 163 4692
Fragments arranged by size:
4692 181 163
BtsI GCAGTG_nn'
Cuts at: 1259 1847 2279 1259
Size: 588 432 4016
Fragments arranged by size:
4016 588 432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1706 1741 1706
Size: 35 5001
DraI TTT'AAA
Cuts at: 242 2035 242
Size: 1793 3243
DrdI GACnn_nn'nnGTC
Cuts at: 2742 4086 2742
Size: 1344 3692
EaeI y'GGCC_r
Cuts at: 753 2621 2795 3186 3213 4558 753
Size: 1868 174 391 27 1345 1231
Fragments arranged by size:
1868 1345 1231 391 174 27
EagI C'GGCC_G
Cuts at: 2621 2621
Size: 5036
EarI CTCTTCn'nnn_
Cuts at: 181 451 665 1100 3059 3269 181
Size: 270 214 435 1959 210 1948
Fragments arranged by size:
1959 1948 435 270 214 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1274 1759 3244 3986 4132 4846 1274
Size: 485 1485 742 146 714 1464
Fragments arranged by size:
1485 1464 742 714 485 146
EcoICRI GAG'CTC
Cuts at: 1420 2048 1420
Size: 628 4408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 1397 1469 1517 1580 2861 3293 3646 4308
Size: 177 72 48 63 1281 432 353 662
Cuts at: 4308 1220
Size: 1948
Fragments arranged by size:
1948 1281 662 432 353 177 72 63
48
EcoNI CCTnn'n_nnAGG
Cuts at: 1520 1520
Size: 5036
EcoO109I rG'GnC_Cy
Cuts at: 1462 1463 2175 1462
Size: 1 712 4323
Fragments arranged by size:
4323 712 1
EcoRI G'AATT_C
Cuts at: 2040 2040
Size: 5036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 5036
FspI TGC'GCA
Cuts at: 2817 2817
Size: 5036
HaeII r_GCGC'y
Cuts at: 2470 2718 3948 4719 2470
Size: 248 1230 771 2787
Fragments arranged by size:
2787 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 237 269 1240 1272 1800 1832 1848 1880 2878
Size: 32 971 32 528 32 16 32 998
Cuts at: 2878 2908 2910 2940 237
Size: 30 2 30 2333
Fragments arranged by size:
2333 998 971 528 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 2075 2075
Size: 5036
KasI G'GCGC_C
Cuts at: 2466 2714 2466
Size: 248 4788
KpnI G_GTAC'C
Cuts at: 2056 2056
Size: 5036
MluI A'CGCG_T
Cuts at: 4469 4469
Size: 5036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 658 3795 3979 578
Size: 80 3137 184 1635
Fragments arranged by size:
3137 1635 184 80
MscI TGG'CCA
Cuts at: 2797 2797
Size: 5036
MslI CAynn'nnrTG
Cuts at: 845 1247 3025 3152 845
Size: 402 1778 127 2729
Fragments arranged by size:
2729 1778 402 127
MspA1I CmG'CkG
Cuts at: 2147 2461 2821 3605 3850 2147
Size: 314 360 784 245 3333
Fragments arranged by size:
3333 784 360 314 245
NaeI GCC'GGC
Cuts at: 3217 3217
Size: 5036
NarI GG'CG_CC
Cuts at: 2467 2715 2467
Size: 248 4788
NcoI C'CATG_G
Cuts at: 107 107
Size: 5036
NgoMIV G'CCGG_C
Cuts at: 3215 3215
Size: 5036
NspI r_CATG'y
Cuts at: 508 547 810 2089 2589 4192 508
Size: 39 263 1279 500 1603 1352
Fragments arranged by size:
1603 1352 1279 500 263 39
PasI CC'CwG_GG
Cuts at: 1666 1699 1666
Size: 33 5003
PciI A'CATG_T
Cuts at: 806 4188 806
Size: 3382 1654
PflMI CCAn_nnn'nTGG
Cuts at: 583 1345 1699 583
Size: 762 354 3920
Fragments arranged by size:
3920 762 354
PfoI T'CCnGG_A
Cuts at: 566 1413 1485 566
Size: 847 72 4117
Fragments arranged by size:
4117 847 72
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 5004
PspOMI G'GGCC_C
Cuts at: 1462 1462
Size: 5036
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 368 400 368
Size: 32 5004
PstI C_TGCA'G
Cuts at: 237 2083 2768 237
Size: 1846 685 2505
Fragments arranged by size:
2505 1846 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5036
PvuII CAG'CTG
Cuts at: 2461 2821 2461
Size: 360 4676
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5036
SacI G_AGCT'C
Cuts at: 1422 2050 1422
Size: 628 4408
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5036
SapI GCTCTTCn'nnn_
Cuts at: 3059 3269 3059
Size: 210 4826
SbfI CC_TGCA'GG
Cuts at: 2083 2083
Size: 5036
ScaI AGT'ACT
Cuts at: 338 918 4553 338
Size: 580 3635 821
Fragments arranged by size:
3635 821 580
SfcI C'TryA_G
Cuts at: 33 233 1388 2079 2764 3732 3923 33
Size: 200 1155 691 685 968 191 1146
Fragments arranged by size:
1155 1146 968 691 685 200 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1380 1380
Size: 5036
SfoI GGC'GCC
Cuts at: 2468 2716 2468
Size: 248 4788
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
SmaI CCC'GGG
Cuts at: 2058 2058
Size: 5036
SmlI C'TyrA_G
Cuts at: 734 2191 3106 3820 4082 734
Size: 1457 915 714 262 1688
Fragments arranged by size:
1688 1457 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5036
SphI G_CATG'C
Cuts at: 2089 2589 2089
Size: 500 4536
SspI AAT'ATT
Cuts at: 478 478
Size: 5036
StuI AGG'CCT
Cuts at: 1394 1394
Size: 5036
StyI C'CwwG_G
Cuts at: 107 1573 1650 1739 1820 2170 2524 107
Size: 1466 77 89 81 350 354 2619
Fragments arranged by size:
2619 1466 354 350 89 81 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1959 2619 2752 3211 3420 1734
Size: 225 660 133 459 209 3350
Fragments arranged by size:
3350 660 459 225 209 133
TatI w'GTAC_w
Cuts at: 336 916 1974 4551 336
Size: 580 1058 2577 821
Fragments arranged by size:
2577 1058 821 580
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 587 619 4553 4585 587
Size: 32 3934 32 1038
Fragments arranged by size:
3934 1038 32 32
Tth111I GACn'n_nGTC
Cuts at: 2833 2833
Size: 5036
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 2531
Size: 5036
XmaI C'CCGG_G
Cuts at: 2056 2056
Size: 5036
XmnI GAAnn'nnTTC
Cuts at: 380 380
Size: 5036
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI
SacII SanDI SexAI SgrAI SnaBI SrfI SwaI XhoI
ZraI