Restriction map for
pF1KB4108
(Circular) (Six-base) MAPSORT of: pF1KB4108.seq Check: 4057 from: 1 to: 6044
pF1KB4108 6044 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2834 3082 2834
Size: 248 5796
Acc65I G'GTAC_C
Cuts at: 2108 3060 2108
Size: 952 5092
AclI AA'CG_TT
Cuts at: 1795 5884 1795
Size: 4089 1955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1369 1551 2309 3869 4301 4654 5316 772
Size: 597 182 758 1560 432 353 662 1500
Fragments arranged by size:
1560 1500 758 662 597 432 353 182
AflII C'TTAA_G
Cuts at: 375 375
Size: 6044
AflIII A'CryG_T
Cuts at: 1102 1514 1949 3396 5196 5477 1102
Size: 412 435 1447 1800 281 1669
Fragments arranged by size:
1800 1669 1447 435 412 281
AleI CACnn'nnGTG
Cuts at: 846 1531 2136 846
Size: 685 605 4754
Fragments arranged by size:
4754 685 605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1799 1799
Size: 6044
AlwNI CAG_nnn'CTG
Cuts at: 795 1035 1818 3349 4787 795
Size: 240 783 1531 1438 2052
Fragments arranged by size:
2052 1531 1438 783 240
ApaLI G'TGCA_C
Cuts at: 4882 4882
Size: 6044
ApoI r'AATT_y
Cuts at: 275 520 814 1602 1879 2014 2424 2484 2616
Size: 245 294 788 277 135 410 60 132
Cuts at: 2616 3048 5397 5653 275
Size: 432 2349 256 666
Fragments arranged by size:
2349 788 666 432 410 294 277 256
245 135 132 60
AseI AT'TA_AT
Cuts at: 20 296 20
Size: 276 5768
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6044
AvaI C'yCGr_G
Cuts at: 2088 2771 3064 2088
Size: 683 293 5068
Fragments arranged by size:
5068 683 293
BamHI G'GATC_C
Cuts at: 3069 3069
Size: 6044
BanI G'GyrC_C
Cuts at: 486 1897 2108 3060 3474 3722 3757 486
Size: 1411 211 952 414 248 35 2773
Fragments arranged by size:
2773 1411 952 414 248 211 35
BanII G_rGCy'C
Cuts at: 362 1841 2095 3058 4088 362
Size: 1479 254 963 1030 2318
Fragments arranged by size:
2318 1479 1030 963 254
BbeI G_GCGC'C
Cuts at: 3478 3726 3478
Size: 248 5796
BbsI GAAGACnn'nnnn_
Cuts at: 1083 1125 2217 2411 1083
Size: 42 1092 194 4716
Fragments arranged by size:
4716 1092 194 42
BciVI GTATCCnnnnn_n'
Cuts at: 3942 4998 6044 3942
Size: 1056 1046 3942
Fragments arranged by size:
3942 1056 1046
BclI T'GATC_A
Cuts at: 2114 3564 2114
Size: 1450 4594
BfrBI ATG'CAT
Cuts at: 1786 1786
Size: 6044
BglII A'GATC_T
Cuts at: 3559 5596 3559
Size: 2037 4007
BlpI GC'TnA_GC
Cuts at: 2678 3156 2678
Size: 478 5566
Bme1580I G_kGCm'C
Cuts at: 1491 3669 3762 4886 1491
Size: 2178 93 1124 2649
Fragments arranged by size:
2649 2178 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1092 2872 3256 3337 3672 5816 1092
Size: 1780 384 81 335 2144 1320
Fragments arranged by size:
2144 1780 1320 384 335 81
BmtI G_CTAG'C
Cuts at: 1693 1693
Size: 6044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4411 4411
Size: 6044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 675 1089 512
Size: 163 414 5467
Fragments arranged by size:
5467 414 163
Bpu10I CC'TnA_GC
Cuts at: 863 2797 863
Size: 1934 4110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2226 2767 3220 4099 4813 5111 2226
Size: 541 453 879 714 298 3159
Fragments arranged by size:
3159 879 714 541 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 146 2815 5748 146
Size: 2669 2933 442
Fragments arranged by size:
2933 2669 442
BsaAI yAC'GTr
Cuts at: 3024 3397 3024
Size: 373 5671
BsaBI GATnn'nnATC
Cuts at: 6 3558 5595 6
Size: 3552 2037 455
Fragments arranged by size:
3552 2037 455
BsaHI Gr'CG_yC
Cuts at: 3475 3723 4425 5921 3475
Size: 248 702 1496 3598
Fragments arranged by size:
3598 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3233 3754 4432 4843 4990 5264 3233
Size: 521 678 411 147 274 4013
Fragments arranged by size:
4013 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 593 623 673 703 1204 1234 593
Size: 30 50 30 501 30 5403
Fragments arranged by size:
5403 501 50 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 1086 319
Size: 767 5277
BseYI C'CCAG_C
Cuts at: 1013 3469 4227 4892 5300 1013
Size: 2456 758 665 408 1757
Fragments arranged by size:
2456 1757 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3586 3632 4862 104
Size: 3482 46 1230 1286
Fragments arranged by size:
3482 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 362 3058 3836 4026 4886 362
Size: 2696 778 190 860 1520
Fragments arranged by size:
2696 1520 860 778 190
BsmI GAATG_Cn'
Cuts at: 225 652 1646 2409 2801 225
Size: 427 994 763 392 3468
Fragments arranged by size:
3468 994 763 427 392
Bsp1286I G_dGCh'C
Cuts at: 362 1491 1841 2095 3058 3669 3762 3836 4026
Size: 1129 350 254 963 611 93 74 190
Cuts at: 4026 4088 4886 362
Size: 62 798 1520
Fragments arranged by size:
1520 1129 963 798 611 350 254 190
93 74 62
BspHI T'CATG_A
Cuts at: 4476 4476
Size: 6044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1759 3094 3308 3610 3991 1759
Size: 1335 214 302 381 3812
Fragments arranged by size:
3812 1335 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4336 4390 5874 6039 50
Size: 4286 54 1484 165 55
Fragments arranged by size:
4286 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 332 731 2273 2568 3956 332
Size: 399 1542 295 1388 2420
Fragments arranged by size:
2420 1542 1388 399 295
BsrFI r'CCGG_y
Cuts at: 4042 4223 4042
Size: 181 5863
BssSI C'ACGA_G
Cuts at: 622 1499 4315 5023 622
Size: 877 2816 708 1643
Fragments arranged by size:
2816 1643 877 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2536 3421 2536
Size: 885 5159
BstBI TT'CG_AA
Cuts at: 4405 4405
Size: 6044
BstEII G'GTnAC_C
Cuts at: 2791 2791
Size: 6044
BstXI CCAn_nnnn'nTGG
Cuts at: 900 1533 3036 900
Size: 633 1503 3908
Fragments arranged by size:
3908 1503 633
BstYI r'GATC_y
Cuts at: 1 750 2123 3069 3536 3559 3894 4140 4446
Size: 749 1373 946 467 23 335 246 306
Cuts at: 4446 4458 4544 5596 1
Size: 12 86 1052 449
Fragments arranged by size:
1373 1052 946 749 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1330 107
Size: 1223 4821
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3994 4175 4338 3994
Size: 181 163 5700
Fragments arranged by size:
5700 181 163
BtsI GCAGTG_nn'
Cuts at: 853 1094 1807 3287 853
Size: 241 713 1480 3610
Fragments arranged by size:
3610 1480 713 241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1890 1925 1890
Size: 35 6009
DraI TTT'AAA
Cuts at: 285 3043 285
Size: 2758 3286
DraIII CAC_nnn'GTG
Cuts at: 2791 2791
Size: 6044
DrdI GACnn_nn'nnGTC
Cuts at: 1812 3750 5094 1812
Size: 1938 1344 2762
Fragments arranged by size:
2762 1938 1344
EaeI y'GGCC_r
Cuts at: 716 980 3629 3803 4194 4221 5566 716
Size: 264 2649 174 391 27 1345 1194
Fragments arranged by size:
2649 1345 1194 391 264 174 27
EagI C'GGCC_G
Cuts at: 3629 3629
Size: 6044
EarI CTCTTCn'nnn_
Cuts at: 827 2300 2957 4067 4277 827
Size: 1473 657 1110 210 2594
Fragments arranged by size:
2594 1473 1110 657 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1394 4252 4994 5140 5854 1394
Size: 2858 742 146 714 1584
Fragments arranged by size:
2858 1584 742 714 146
EcoICRI GAG'CTC
Cuts at: 360 3056 360
Size: 2696 3348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 675 772 1089 1369 1551 2309 3869 4301
Size: 163 97 317 280 182 758 1560 432
Cuts at: 4301 4654 5316 512
Size: 353 662 1240
Fragments arranged by size:
1560 1240 758 662 432 353 317 280
182 163 97
EcoO109I rG'GnC_Cy
Cuts at: 867 1335 1615 3183 867
Size: 468 280 1568 3728
Fragments arranged by size:
3728 1568 468 280
EcoRI G'AATT_C
Cuts at: 520 814 3048 520
Size: 294 2234 3516
Fragments arranged by size:
3516 2234 294
EcoRV GAT'ATC
Cuts at: 2631 2631
Size: 6044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4063 4063
Size: 6044
FspI TGC'GCA
Cuts at: 3825 3825
Size: 6044
HaeII r_GCGC'y
Cuts at: 3478 3726 4956 5727 3478
Size: 248 1230 771 3795
Fragments arranged by size:
3795 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 686 718 1166 1198 2199 2231 2874
Size: 32 511 32 448 32 1001 32 643
Cuts at: 2874 2906 3886 3916 3918 3948 143
Size: 32 980 30 2 30 2239
Fragments arranged by size:
2239 1001 980 643 511 448 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1027 1748 2728 3083 1027
Size: 721 980 355 3988
Fragments arranged by size:
3988 980 721 355
HindIII A'AGCT_T
Cuts at: 378 2029 378
Size: 1651 4393
HpaI GTT'AAC
Cuts at: 1748 1748
Size: 6044
KasI G'GCGC_C
Cuts at: 3474 3722 3474
Size: 248 5796
KpnI G_GTAC'C
Cuts at: 2112 3064 2112
Size: 952 5092
MfeI C'AATT_G
Cuts at: 338 1020 1714 2534 338
Size: 682 694 820 3848
Fragments arranged by size:
3848 820 694 682
MluI A'CGCG_T
Cuts at: 5477 5477
Size: 6044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 375 414 803 4803 4987 375
Size: 39 389 4000 184 1432
Fragments arranged by size:
4000 1432 389 184 39
MscI TGG'CCA
Cuts at: 718 982 3805 718
Size: 264 2823 2957
Fragments arranged by size:
2957 2823 264
MslI CAynn'nnrTG
Cuts at: 846 1531 2136 4033 4160 846
Size: 685 605 1897 127 2730
Fragments arranged by size:
2730 1897 685 605 127
MspA1I CmG'CkG
Cuts at: 632 1032 3155 3469 3829 4613 4858 632
Size: 400 2123 314 360 784 245 1818
Fragments arranged by size:
2123 1818 784 400 360 314 245
NaeI GCC'GGC
Cuts at: 4225 4225
Size: 6044
NarI GG'CG_CC
Cuts at: 3475 3723 3475
Size: 248 5796
NcoI C'CATG_G
Cuts at: 107 107
Size: 6044
NdeI CA'TA_TG
Cuts at: 1348 1348
Size: 6044
NgoMIV G'CCGG_C
Cuts at: 4223 4223
Size: 6044
NheI G'CTAG_C
Cuts at: 1689 1689
Size: 6044
NsiI A_TGCA'T
Cuts at: 1788 1788
Size: 6044
NspI r_CATG'y
Cuts at: 415 1106 1518 1953 2956 3097 3597 5200 415
Size: 691 412 435 1003 141 500 1603 1259
Fragments arranged by size:
1603 1259 1003 691 500 435 412 141
PasI CC'CwG_GG
Cuts at: 2594 2594
Size: 6044
PciI A'CATG_T
Cuts at: 1102 1514 1949 5196 1102
Size: 412 435 3247 1950
Fragments arranged by size:
3247 1950 435 412
PfoI T'CCnGG_A
Cuts at: 1646 1646
Size: 6044
PmeI GTTT'AAAC
Cuts at: 3043 3043
Size: 6044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 6012
PpuMI rG'GwC_Cy
Cuts at: 867 1335 1615 867
Size: 468 280 5296
Fragments arranged by size:
5296 468 280
PshAI GACnn'nnGTC
Cuts at: 932 1861 932
Size: 929 5115
PstI C_TGCA'G
Cuts at: 1814 2739 3091 3776 1814
Size: 925 352 685 4082
Fragments arranged by size:
4082 925 685 352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6044
PvuII CAG'CTG
Cuts at: 1032 3469 3829 1032
Size: 2437 360 3247
Fragments arranged by size:
3247 2437 360
RsrII CG'GwC_CG
Cuts at: 4239 4239
Size: 6044
SacI G_AGCT'C
Cuts at: 362 3058 362
Size: 2696 3348
SalI G'TCGA_C
Cuts at: 3081 3081
Size: 6044
SapI GCTCTTCn'nnn_
Cuts at: 827 4067 4277 827
Size: 3240 210 2594
Fragments arranged by size:
3240 2594 210
SbfI CC_TGCA'GG
Cuts at: 3091 3091
Size: 6044
ScaI AGT'ACT
Cuts at: 5561 5561
Size: 6044
SexAI A'CCwGG_T
Cuts at: 2243 2243
Size: 6044
SfcI C'TryA_G
Cuts at: 33 147 1810 2735 3087 3772 4740 4931 33
Size: 114 1663 925 352 685 968 191 1146
Fragments arranged by size:
1663 1146 968 925 685 352 191 114
SfoI GGC'GCC
Cuts at: 3476 3724 3476
Size: 248 5796
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6044
SmaI CCC'GGG
Cuts at: 3066 3066
Size: 6044
SmlI C'TyrA_G
Cuts at: 375 2205 2746 2771 3199 4114 4828 5090 375
Size: 1830 541 25 428 915 714 262 1329
Fragments arranged by size:
1830 1329 915 714 541 428 262 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6044
SphI G_CATG'C
Cuts at: 3097 3597 3097
Size: 500 5544
SspI AAT'ATT
Cuts at: 294 1832 2757 294
Size: 1538 925 3581
Fragments arranged by size:
3581 1538 925
StuI AGG'CCT
Cuts at: 712 2297 712
Size: 1585 4459
StyI C'CwwG_G
Cuts at: 107 713 3178 3532 107
Size: 606 2465 354 2619
Fragments arranged by size:
2619 2465 606 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2317 2641 3025 3627 3760 4219 4428 2317
Size: 324 384 602 133 459 209 3933
Fragments arranged by size:
3933 602 459 384 324 209 133
TatI w'GTAC_w
Cuts at: 1481 1718 5559 1481
Size: 237 3841 1966
Fragments arranged by size:
3841 1966 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 593 625 673 705 1204 1236 2870 2902 5561
Size: 32 48 32 499 32 1634 32 2659
Cuts at: 5561 5593 593
Size: 32 1044
Fragments arranged by size:
2659 1634 1044 499 48 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3004 3841 3004
Size: 837 5207
XbaI T'CTAG_A
Cuts at: 3075 3075
Size: 6044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1505 3539 663
Size: 842 2034 3168
Fragments arranged by size:
3168 2034 842
XhoI C'TCGA_G
Cuts at: 2771 2771
Size: 6044
XmaI C'CCGG_G
Cuts at: 3064 3064
Size: 6044
XmnI GAAnn'nnTTC
Cuts at: 908 908
Size: 6044
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI EcoNI
FseI FspAI NotI NruI PacI PflMI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI ZraI