Restriction map for
pF1KB4113
(Circular) (Six-base) MAPSORT of: pF1KB4113.seq Check: 467 from: 1 to: 4685
pF1KB4113 4685 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1424 1637 1723 1424
Size: 213 86 4386
Fragments arranged by size:
4386 213 86
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 530 1160 1559 2510 2942 3295 3957 172
Size: 358 630 399 951 432 353 662 900
Fragments arranged by size:
951 900 662 630 432 399 358 353
AfeI AGC'GCT
Cuts at: 145 145
Size: 4685
AflIII A'CryG_T
Cuts at: 2037 3837 4118 2037
Size: 1800 281 2604
Fragments arranged by size:
2604 1800 281
AgeI A'CCGG_T
Cuts at: 1439 1439
Size: 4685
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 203 235 203
Size: 32 4653
AlwNI CAG_nnn'CTG
Cuts at: 1990 3428 1990
Size: 1438 3247
ApaLI G'TGCA_C
Cuts at: 3523 3523
Size: 4685
ApoI r'AATT_y
Cuts at: 253 278 809 1689 4038 4294 253
Size: 25 531 880 2349 256 644
Fragments arranged by size:
2349 880 644 531 256 25
AseI AT'TA_AT
Cuts at: 20 316 20
Size: 296 4389
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 1705 1705
Size: 4685
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 1701 2115 2363 2398 1701
Size: 414 248 35 3988
Fragments arranged by size:
3988 414 248 35
BanII G_rGCy'C
Cuts at: 1699 2729 1699
Size: 1030 3655
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbsI GAAGACnn'nnnn_
Cuts at: 935 1598 935
Size: 663 4022
BciVI GTATCCnnnnn_n'
Cuts at: 375 2583 3639 4685 375
Size: 2208 1056 1046 375
Fragments arranged by size:
2208 1056 1046 375
BclI T'GATC_A
Cuts at: 1412 2205 1412
Size: 793 3892
BglII A'GATC_T
Cuts at: 537 2200 4237 537
Size: 1663 2037 985
Fragments arranged by size:
2037 1663 985
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 1343 2310 2403 3527 1343
Size: 967 93 1124 2501
Fragments arranged by size:
2501 1124 967 93
BmrI ACTGGGnnnn_n'
Cuts at: 171 942 989 1897 1978 2313 4457 171
Size: 771 47 908 81 335 2144 399
Fragments arranged by size:
2144 908 771 399 335 81 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1187 3052 1187
Size: 1865 2820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1537 293
Size: 1244 3441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3454 3752 1861
Size: 879 714 298 2794
Fragments arranged by size:
2794 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4389 4389
Size: 4685
BsaAI yAC'GTr
Cuts at: 2038 2038
Size: 4685
BsaBI GATnn'nnATC
Cuts at: 6 2199 4236 6
Size: 2193 2037 455
Fragments arranged by size:
2193 2037 455
BsaHI Gr'CG_yC
Cuts at: 2116 2364 3066 4562 2116
Size: 248 702 1496 2239
Fragments arranged by size:
2239 1496 702 248
BsaWI w'CCGG_w
Cuts at: 367 1439 1874 2395 3073 3484 3631 3905 367
Size: 1072 435 521 678 411 147 274 1147
Fragments arranged by size:
1147 1072 678 521 435 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 203 233 203
Size: 30 4655
BseYI C'CCAG_C
Cuts at: 622 907 951 2110 2868 3533 3941 622
Size: 285 44 1159 758 665 408 1366
Fragments arranged by size:
1366 1159 758 665 408 285 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 4685
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3503 104
Size: 2123 46 1230 1286
Fragments arranged by size:
2123 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1699 2477 2667 3527 1699
Size: 778 190 860 2857
Fragments arranged by size:
2857 860 778 190
BsiWI C'GTAC_G
Cuts at: 1032 1032
Size: 4685
Bsp1286I G_dGCh'C
Cuts at: 1343 1699 2310 2403 2477 2667 2729 3527 1343
Size: 356 611 93 74 190 62 798 2501
Fragments arranged by size:
2501 798 611 356 190 93 74 62
BspEI T'CCGG_A
Cuts at: 367 367
Size: 4685
BspHI T'CATG_A
Cuts at: 668 1091 3117 668
Size: 423 2026 2236
Fragments arranged by size:
2236 2026 423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1735 1949 2251 2632 1735
Size: 214 302 381 3788
Fragments arranged by size:
3788 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2977 3031 4515 4680 50
Size: 2927 54 1484 165 55
Fragments arranged by size:
2927 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 416 1415 2597 416
Size: 999 1182 2504
Fragments arranged by size:
2504 1182 999
BsrFI r'CCGG_y
Cuts at: 117 1439 2683 2864 117
Size: 1322 1244 181 1938
Fragments arranged by size:
1938 1322 1244 181
BssHII G'CGCG_C
Cuts at: 384 384
Size: 4685
BssSI C'ACGA_G
Cuts at: 2956 3664 2956
Size: 708 3977
BstAPI GCAn_nnn'nTGC
Cuts at: 2062 2062
Size: 4685
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstEII G'GTnAC_C
Cuts at: 1356 1356
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1065
Size: 4685
BstYI r'GATC_y
Cuts at: 1 537 1710 2177 2200 2535 2781 3087 3099
Size: 536 1173 467 23 335 246 306 12
Cuts at: 3099 3185 4237 1
Size: 86 1052 449
Fragments arranged by size:
1173 1052 536 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 179 185 1058 1361 1382 1451 107
Size: 72 6 873 303 21 69 3341
Fragments arranged by size:
3341 873 303 72 69 21 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4341
Fragments arranged by size:
4341 181 163
BtsI GCAGTG_nn'
Cuts at: 1114 1238 1928 1114
Size: 124 690 3871
Fragments arranged by size:
3871 690 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 399 434 1563 1598 399
Size: 35 1129 35 3486
Fragments arranged by size:
3486 1129 35 35
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DrdI GACnn_nn'nnGTC
Cuts at: 651 2391 3735 651
Size: 1740 1344 1601
Fragments arranged by size:
1740 1601 1344
EaeI y'GGCC_r
Cuts at: 182 965 1307 2270 2444 2835 2862 4207 182
Size: 783 342 963 174 391 27 1345 660
Fragments arranged by size:
1345 963 783 660 391 342 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 644 1104 2708 2918 644
Size: 460 1604 210 2411
Fragments arranged by size:
2411 1604 460 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3635 3781 4495 2893
Size: 742 146 714 3083
Fragments arranged by size:
3083 742 714 146
EcoICRI GAG'CTC
Cuts at: 1697 1697
Size: 4685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 293 530 1160 1537 1559 2510 2942 3295
Size: 121 237 630 377 22 951 432 353
Cuts at: 3295 3957 172
Size: 662 900
Fragments arranged by size:
951 900 662 630 432 377 353 237
121 22
EcoO109I rG'GnC_Cy
Cuts at: 1824 1824
Size: 4685
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 147 2119 2367 3597 4368 147
Size: 1972 248 1230 771 464
Fragments arranged by size:
1972 1230 771 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1519 1551 1639 1671 2527 2557 2559 2589 1519
Size: 32 88 32 856 30 2 30 3615
Fragments arranged by size:
3615 856 88 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1425 1458 1545 1724 1425
Size: 33 87 179 4386
Fragments arranged by size:
4386 179 87 33
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 383 3444 3628 383
Size: 3061 184 1440
Fragments arranged by size:
3061 1440 184
MscI TGG'CCA
Cuts at: 967 2446 967
Size: 1479 3206
MslI CAynn'nnrTG
Cuts at: 1063 2674 2801 1063
Size: 1611 127 2947
Fragments arranged by size:
2947 1611 127
MspA1I CmG'CkG
Cuts at: 187 664 1014 1133 1240 1796 2110 2470 3254
Size: 477 350 119 107 556 314 360 784
Cuts at: 3254 3499 187
Size: 245 1373
Fragments arranged by size:
1373 784 556 477 360 350 314 245
119 107
NaeI GCC'GGC
Cuts at: 2866 2866
Size: 4685
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NcoI C'CATG_G
Cuts at: 107 1058 1361 1451 107
Size: 951 303 90 3341
Fragments arranged by size:
3341 951 303 90
NgoMIV G'CCGG_C
Cuts at: 2864 2864
Size: 4685
NspI r_CATG'y
Cuts at: 723 1738 2238 3841 723
Size: 1015 500 1603 1567
Fragments arranged by size:
1603 1567 1015 500
PciI A'CATG_T
Cuts at: 3837 3837
Size: 4685
PflMI CCAn_nnn'nTGG
Cuts at: 477 477
Size: 4685
PfoI T'CCnGG_A
Cuts at: 270 1433 270
Size: 1163 3522
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 203 235 3131 3163 203
Size: 32 2896 32 1725
Fragments arranged by size:
2896 1725 32 32
PshAI GACnn'nnGTC
Cuts at: 1453 1453
Size: 4685
PstI C_TGCA'G
Cuts at: 1732 2417 1732
Size: 685 4000
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 664 2110 2470 664
Size: 1446 360 2879
Fragments arranged by size:
2879 1446 360
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 1699 1699
Size: 4685
SacII CC_GC'GG
Cuts at: 188 188
Size: 4685
SalI G'TCGA_C
Cuts at: 1423 1722 1423
Size: 299 4386
SapI GCTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SexAI A'CCwGG_T
Cuts at: 1151 1151
Size: 4685
SfcI C'TryA_G
Cuts at: 33 1728 2413 3381 3572 33
Size: 1695 685 968 191 1146
Fragments arranged by size:
1695 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4685
SmlI C'TyrA_G
Cuts at: 1840 2755 3469 3731 1840
Size: 915 714 262 2794
Fragments arranged by size:
2794 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
SspI AAT'ATT
Cuts at: 941 941
Size: 4685
StuI AGG'CCT
Cuts at: 577 577
Size: 4685
StyI C'CwwG_G
Cuts at: 107 1058 1361 1451 1464 1565 1819 2173 107
Size: 951 303 90 13 101 254 354 2619
Fragments arranged by size:
2619 951 354 303 254 101 90 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 469 484 1678 2268 2401 2860 3069 153
Size: 316 15 1194 590 133 459 209 1769
Fragments arranged by size:
1769 1194 590 459 316 209 133 15
TatI w'GTAC_w
Cuts at: 590 4200 590
Size: 3610 1075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4202 4234 4202
Size: 32 4653
Tth111I GACn'n_nGTC
Cuts at: 2482 2482
Size: 4685
XbaI T'CTAG_A
Cuts at: 246 1716 246
Size: 1470 3215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 213 1512 2180 213
Size: 1299 668 2718
Fragments arranged by size:
2718 1299 668
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4685
XmnI GAAnn'nnTTC
Cuts at: 145 553 1045 145
Size: 408 492 3785
Fragments arranged by size:
3785 492 408
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PsiI PspOMI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI