Restriction map for
pF1KB4115
(Circular) (Six-base) MAPSORT of: pF1KB4115.seq Check: 9179 from: 1 to: 5927
pF1KB4115 5927 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1263 2965 1263
Size: 1702 4225
Acc65I G'GTAC_C
Cuts at: 2943 2943
Size: 5927
AclI AA'CG_TT
Cuts at: 362 5767 362
Size: 5405 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1210 1583 1661 2465 3752 4184 4537 5199
Size: 719 373 78 804 1287 432 353 662
Cuts at: 5199 491
Size: 1219
Fragments arranged by size:
1287 1219 804 719 662 432 373 353
78
AflIII A'CryG_T
Cuts at: 1974 3279 5079 5360 1974
Size: 1305 1800 281 2541
Fragments arranged by size:
2541 1800 1305 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1151 1151
Size: 5927
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 182 214 182
Size: 32 5895
AlwNI CAG_nnn'CTG
Cuts at: 3232 4670 3232
Size: 1438 4489
ApaLI G'TGCA_C
Cuts at: 4765 4765
Size: 5927
ApoI r'AATT_y
Cuts at: 1588 2319 2931 5280 5536 1588
Size: 731 612 2349 256 1979
Fragments arranged by size:
2349 1979 731 612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5927
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5927
AvaI C'yCGr_G
Cuts at: 2947 2947
Size: 5927
AvrII C'CTAG_G
Cuts at: 2079 2079
Size: 5927
BamHI G'GATC_C
Cuts at: 2952 2952
Size: 5927
BanI G'GyrC_C
Cuts at: 2428 2943 3357 3605 3640 2428
Size: 515 414 248 35 4715
Fragments arranged by size:
4715 515 414 248 35
BanII G_rGCy'C
Cuts at: 2110 2460 2801 2941 3971 2110
Size: 350 341 140 1030 4066
Fragments arranged by size:
4066 1030 350 341 140
BbeI G_GCGC'C
Cuts at: 3361 3609 3361
Size: 248 5679
BbsI GAAGACnn'nnnn_
Cuts at: 647 647
Size: 5927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 892 926 892
Size: 34 5893
BciVI GTATCCnnnnn_n'
Cuts at: 2629 3825 4881 5927 2629
Size: 1196 1056 1046 2629
Fragments arranged by size:
2629 1196 1056 1046
BclI T'GATC_A
Cuts at: 717 3447 717
Size: 2730 3197
BglI GCCn_nnn'nGGC
Cuts at: 226 226
Size: 5927
BglII A'GATC_T
Cuts at: 807 1995 3442 5479 807
Size: 1188 1447 2037 1255
Fragments arranged by size:
2037 1447 1255 1188
BlpI GC'TnA_GC
Cuts at: 3039 3039
Size: 5927
Bme1580I G_kGCm'C
Cuts at: 2433 3552 3645 4769 2433
Size: 1119 93 1124 3591
Fragments arranged by size:
3591 1124 1119 93
BmgBI CAC'GTC
Cuts at: 2605 2605
Size: 5927
BmrI ACTGGGnnnn_n'
Cuts at: 1750 2516 3139 3220 3555 5699 1750
Size: 766 623 81 335 2144 1978
Fragments arranged by size:
2144 1978 766 623 335 81
BmtI G_CTAG'C
Cuts at: 1651 1651
Size: 5927
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4294 4294
Size: 5927
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3103 3982 4696 4994 3103
Size: 879 714 298 4036
Fragments arranged by size:
4036 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 840 5631 840
Size: 4791 1136
BsaAI yAC'GTr
Cuts at: 3280 3280
Size: 5927
BsaBI GATnn'nnATC
Cuts at: 6 446 1703 2675 3441 5478 6
Size: 440 1257 972 766 2037 455
Fragments arranged by size:
2037 1257 972 766 455 440
BsaHI Gr'CG_yC
Cuts at: 1578 3358 3606 4308 5804 1578
Size: 1780 248 702 1496 1701
Fragments arranged by size:
1780 1701 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1949 3116 3637 4315 4726 4873 5147 1949
Size: 1167 521 678 411 147 274 2729
Fragments arranged by size:
2729 1167 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 182 212 182
Size: 30 5897
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1208 1603 2087 1208
Size: 395 484 5048
Fragments arranged by size:
5048 484 395
BseYI C'CCAG_C
Cuts at: 175 2284 3352 4110 4775 5183 175
Size: 2109 1068 758 665 408 919
Fragments arranged by size:
2109 1068 919 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 1507 2327 830
Size: 677 820 4430
Fragments arranged by size:
4430 820 677
BsiEI CG_ry'CG
Cuts at: 104 3469 3515 4745 104
Size: 3365 46 1230 1286
Fragments arranged by size:
3365 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2941 3719 3909 4769 2941
Size: 778 190 860 4099
Fragments arranged by size:
4099 860 778 190
BsmI GAATG_Cn'
Cuts at: 460 2016 460
Size: 1556 4371
Bsp1286I G_dGCh'C
Cuts at: 2110 2433 2460 2801 2941 3552 3645 3719 3909
Size: 323 27 341 140 611 93 74 190
Cuts at: 3909 3971 4769 2110
Size: 62 798 3268
Fragments arranged by size:
3268 798 611 341 323 190 140 93
74 62 27
BspEI T'CCGG_A
Cuts at: 1949 1949
Size: 5927
BspHI T'CATG_A
Cuts at: 4359 4359
Size: 5927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2977 3191 3493 3874 2977
Size: 214 302 381 5030
Fragments arranged by size:
5030 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1882 2094 4219 4273 5757 5922 50
Size: 1832 212 2125 54 1484 165 55
Fragments arranged by size:
2125 1832 1484 212 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2207 3839 2009
Size: 198 1632 4097
Fragments arranged by size:
4097 1632 198
BsrFI r'CCGG_y
Cuts at: 2714 3925 4106 2714
Size: 1211 181 4535
Fragments arranged by size:
4535 1211 181
BssSI C'ACGA_G
Cuts at: 1835 4198 4906 1835
Size: 2363 708 2856
Fragments arranged by size:
2856 2363 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3304 3304
Size: 5927
BstBI TT'CG_AA
Cuts at: 4288 4288
Size: 5927
BstEII G'GTnAC_C
Cuts at: 1939 1939
Size: 5927
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 2632
Size: 5927
BstYI r'GATC_y
Cuts at: 1 295 807 1130 1995 2952 3419 3442 3777
Size: 294 512 323 865 957 467 23 335
Cuts at: 3777 4023 4329 4341 4427 5479 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 957 865 512 467 449 335 323
306 294 246 86 23 12
BtgI C'CryG_G
Cuts at: 1692 1785 2625 1692
Size: 93 840 4994
Fragments arranged by size:
4994 840 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 455 3877 4058 4221 455
Size: 3422 181 163 2161
Fragments arranged by size:
3422 2161 181 163
BtsI GCAGTG_nn'
Cuts at: 1100 3170 1100
Size: 2070 3857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1758 1793 2406 2441 1758
Size: 35 613 35 5244
Fragments arranged by size:
5244 613 35 35
DraI TTT'AAA
Cuts at: 2926 2926
Size: 5927
DrdI GACnn_nn'nnGTC
Cuts at: 1756 2371 2445 3633 4977 1756
Size: 615 74 1188 1344 2706
Fragments arranged by size:
2706 1344 1188 615 74
EaeI y'GGCC_r
Cuts at: 1181 1451 1766 1866 2673 3512 3686 4077 4104
Size: 270 315 100 807 839 174 391 27
Cuts at: 4104 5449 1181
Size: 1345 1659
Fragments arranged by size:
1659 1345 839 807 391 315 270 174
100 27
EagI C'GGCC_G
Cuts at: 3512 3512
Size: 5927
EarI CTCTTCn'nnn_
Cuts at: 243 496 557 629 710 767 1602 1637 1872
Size: 253 61 72 81 57 835 35 235
Cuts at: 1872 3950 4160 243
Size: 2078 210 2010
Fragments arranged by size:
2078 2010 835 253 235 210 81 72
61 57 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4135 4877 5023 5737 4135
Size: 742 146 714 4325
Fragments arranged by size:
4325 742 714 146
EcoICRI GAG'CTC
Cuts at: 2939 2939
Size: 5927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1210 1583 1661 2465 3752 4184 4537 5199
Size: 719 373 78 804 1287 432 353 662
Cuts at: 5199 491
Size: 1219
Fragments arranged by size:
1287 1219 804 719 662 432 373 353
78
EcoNI CCTnn'n_nnAGG
Cuts at: 857 1228 1663 2171 857
Size: 371 435 508 4613
Fragments arranged by size:
4613 508 435 371
EcoO109I rG'GnC_Cy
Cuts at: 1221 3066 1221
Size: 1845 4082
EcoRI G'AATT_C
Cuts at: 2319 2931 2319
Size: 612 5315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1328 2253 3946 1328
Size: 925 1693 3309
Fragments arranged by size:
3309 1693 925
FspI TGC'GCA
Cuts at: 3708 3708
Size: 5927
HaeII r_GCGC'y
Cuts at: 3361 3609 4839 5610 3361
Size: 248 1230 771 3678
Fragments arranged by size:
3678 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 720 752 1089 1121 2568 2600 3769 3799 3801
Size: 32 337 32 1447 32 1169 30 2
Cuts at: 3801 3831 720
Size: 30 2816
Fragments arranged by size:
2816 1447 1169 337 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1264 2469 2966 1264
Size: 1205 497 4225
Fragments arranged by size:
4225 1205 497
HindIII A'AGCT_T
Cuts at: 581 2771 581
Size: 2190 3737
KasI G'GCGC_C
Cuts at: 3357 3605 3357
Size: 248 5679
KpnI G_GTAC'C
Cuts at: 2947 2947
Size: 5927
MfeI C'AATT_G
Cuts at: 674 1088 674
Size: 414 5513
MluI A'CGCG_T
Cuts at: 5360 5360
Size: 5927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1049 2353 2728 4686 4870 1049
Size: 1304 375 1958 184 2106
Fragments arranged by size:
2106 1958 1304 375 184
MscI TGG'CCA
Cuts at: 1453 1768 2675 3688 1453
Size: 315 907 1013 3692
Fragments arranged by size:
3692 1013 907 315
MslI CAynn'nnrTG
Cuts at: 2612 2630 2669 3916 4043 2612
Size: 18 39 1247 127 4496
Fragments arranged by size:
4496 1247 127 39 18
MspA1I CmG'CkG
Cuts at: 2866 3038 3352 3712 4496 4741 2866
Size: 172 314 360 784 245 4052
Fragments arranged by size:
4052 784 360 314 245 172
NaeI GCC'GGC
Cuts at: 2716 4108 2716
Size: 1392 4535
NarI GG'CG_CC
Cuts at: 3358 3606 3358
Size: 248 5679
NcoI C'CATG_G
Cuts at: 1692 2625 1692
Size: 933 4994
NgoMIV G'CCGG_C
Cuts at: 2714 4106 2714
Size: 1392 4535
NheI G'CTAG_C
Cuts at: 1647 1647
Size: 5927
NspI r_CATG'y
Cuts at: 2980 3480 5083 2980
Size: 500 1603 3824
Fragments arranged by size:
3824 1603 500
PciI A'CATG_T
Cuts at: 5079 5079
Size: 5927
PflMI CCAn_nnn'nTGG
Cuts at: 113 616 1081 2404 113
Size: 503 465 1323 3636
Fragments arranged by size:
3636 1323 503 465
PmeI GTTT'AAAC
Cuts at: 2926 2926
Size: 5927
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 182 214 4373 4405 182
Size: 32 4159 32 1704
Fragments arranged by size:
4159 1704 32 32
PstI C_TGCA'G
Cuts at: 1023 2974 3659 1023
Size: 1951 685 3291
Fragments arranged by size:
3291 1951 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5927
PvuII CAG'CTG
Cuts at: 2866 3352 3712 2866
Size: 486 360 5081
Fragments arranged by size:
5081 486 360
RsrII CG'GwC_CG
Cuts at: 4122 4122
Size: 5927
SacI G_AGCT'C
Cuts at: 2941 2941
Size: 5927
SalI G'TCGA_C
Cuts at: 1262 2964 1262
Size: 1702 4225
SapI GCTCTTCn'nnn_
Cuts at: 243 1637 1872 3950 4160 243
Size: 1394 235 2078 210 2010
Fragments arranged by size:
2078 2010 1394 235 210
SbfI CC_TGCA'GG
Cuts at: 2974 2974
Size: 5927
ScaI AGT'ACT
Cuts at: 5444 5444
Size: 5927
SexAI A'CCwGG_T
Cuts at: 1074 1943 1074
Size: 869 5058
SfcI C'TryA_G
Cuts at: 33 1019 2085 2970 3655 4623 4814 33
Size: 986 1066 885 685 968 191 1146
Fragments arranged by size:
1146 1066 986 968 885 685 191
SfoI GGC'GCC
Cuts at: 3359 3607 3359
Size: 248 5679
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5927
SmaI CCC'GGG
Cuts at: 2949 2949
Size: 5927
SmlI C'TyrA_G
Cuts at: 3082 3997 4711 4973 3082
Size: 915 714 262 4036
Fragments arranged by size:
4036 915 714 262
SpeI A'CTAG_T
Cuts at: 67 2302 67
Size: 2235 3692
SphI G_CATG'C
Cuts at: 2980 3480 2980
Size: 500 5427
StuI AGG'CCT
Cuts at: 2078 2158 2078
Size: 80 5847
StyI C'CwwG_G
Cuts at: 452 1217 1692 1919 2079 2387 2460 2625 3061
Size: 765 475 227 160 308 73 165 436
Cuts at: 3061 3415 452
Size: 354 2964
Fragments arranged by size:
2964 765 475 436 354 308 227 165
160 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2285 3510 3643 4102 4311 2285
Size: 1225 133 459 209 3901
Fragments arranged by size:
3901 1225 459 209 133
TatI w'GTAC_w
Cuts at: 2225 5442 2225
Size: 3217 2710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 108 140 5444 5476 108
Size: 32 5304 32 559
Fragments arranged by size:
5304 559 32 32
Tth111I GACn'n_nGTC
Cuts at: 1945 3724 1945
Size: 1779 4148
XbaI T'CTAG_A
Cuts at: 648 2958 648
Size: 2310 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 220 2178 3422 220
Size: 1958 1244 2725
Fragments arranged by size:
2725 1958 1244
XmaI C'CCGG_G
Cuts at: 2947 2947
Size: 5927
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI ApaI
AscI BaeI BbvCI BfrBI BpmI Bpu10I BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI NdeI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI