Restriction map for
pF1KB4121
(Circular) (Six-base) MAPSORT of: pF1KB4121.seq Check: 4175 from: 1 to: 4922
pF1KB4121 4922 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 175 175
Size: 4922
AccI GT'mk_AC
Cuts at: 1135 1960 1135
Size: 825 4097
Acc65I G'GTAC_C
Cuts at: 1938 1938
Size: 4922
AclI AA'CG_TT
Cuts at: 4762 4762
Size: 4922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1187 2747 3179 3532 4194 1187
Size: 1560 432 353 662 1915
Fragments arranged by size:
1915 1560 662 432 353
AflIII A'CryG_T
Cuts at: 527 2274 4074 4355 527
Size: 1747 1800 281 1094
Fragments arranged by size:
1800 1747 1094 281
AlwNI CAG_nnn'CTG
Cuts at: 2227 3665 2227
Size: 1438 3484
ApaLI G'TGCA_C
Cuts at: 3760 3760
Size: 4922
ApoI r'AATT_y
Cuts at: 420 779 917 1357 1477 1630 1926 4275 4531
Size: 359 138 440 120 153 296 2349 256
Cuts at: 4531 420
Size: 811
Fragments arranged by size:
2349 811 440 359 296 256 153 138
120
AseI AT'TA_AT
Cuts at: 20 671 20
Size: 651 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4922
AvaI C'yCGr_G
Cuts at: 176 378 1562 1942 176
Size: 202 1184 380 3156
Fragments arranged by size:
3156 1184 380 202
BamHI G'GATC_C
Cuts at: 1947 1947
Size: 4922
BanI G'GyrC_C
Cuts at: 1938 2352 2600 2635 1938
Size: 414 248 35 4225
Fragments arranged by size:
4225 414 248 35
BanII G_rGCy'C
Cuts at: 689 1787 1936 2966 689
Size: 1098 149 1030 2645
Fragments arranged by size:
2645 1098 1030 149
BbeI G_GCGC'C
Cuts at: 2356 2604 2356
Size: 248 4674
BciVI GTATCCnnnnn_n'
Cuts at: 2820 3876 4922 2820
Size: 1056 1046 2820
Fragments arranged by size:
2820 1056 1046
BclI T'GATC_A
Cuts at: 2442 2442
Size: 4922
BfrBI ATG'CAT
Cuts at: 1006 1006
Size: 4922
BglI GCCn_nnn'nGGC
Cuts at: 973 973
Size: 4922
BglII A'GATC_T
Cuts at: 195 2437 4474 195
Size: 2242 2037 643
Fragments arranged by size:
2242 2037 643
BlpI GC'TnA_GC
Cuts at: 1256 2034 1256
Size: 778 4144
Bme1580I G_kGCm'C
Cuts at: 2547 2640 3764 2547
Size: 93 1124 3705
Fragments arranged by size:
3705 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2134 2215 2550 4694 2134
Size: 81 335 2144 2362
Fragments arranged by size:
2362 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 191 3289 191
Size: 3098 1824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 1408
Size: 4922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1840 2098 2977 3691 3989 1840
Size: 258 879 714 298 2773
Fragments arranged by size:
2773 879 714 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 4626 4626
Size: 4922
BsaAI yAC'GTr
Cuts at: 2275 2275
Size: 4922
BsaBI GATnn'nnATC
Cuts at: 6 1007 2436 4473 6
Size: 1001 1429 2037 455
Fragments arranged by size:
2037 1429 1001 455
BsaHI Gr'CG_yC
Cuts at: 172 2353 2601 3303 4799 172
Size: 2181 248 702 1496 295
Fragments arranged by size:
2181 1496 702 295 248
BsaWI w'CCGG_w
Cuts at: 746 2111 2632 3310 3721 3868 4142 746
Size: 1365 521 678 411 147 274 1526
Fragments arranged by size:
1526 1365 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 142
Size: 30 4892
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 428 998 428
Size: 570 4352
BseYI C'CCAG_C
Cuts at: 949 2347 3105 3770 4178 949
Size: 1398 758 665 408 1693
Fragments arranged by size:
1693 1398 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2464 2510 3740 104
Size: 2360 46 1230 1286
Fragments arranged by size:
2360 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 689 1787 1936 2714 2904 3764 689
Size: 1098 149 778 190 860 1847
Fragments arranged by size:
1847 1098 860 778 190 149
BsmBI CGTCTCn'nnnn_
Cuts at: 179 179
Size: 4922
Bsp1286I G_dGCh'C
Cuts at: 689 1787 1936 2547 2640 2714 2904 2966 3764
Size: 1098 149 611 93 74 190 62 798
Cuts at: 3764 689
Size: 1847
Fragments arranged by size:
1847 1098 798 611 190 149 93 74
62
BspEI T'CCGG_A
Cuts at: 746 746
Size: 4922
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1972 2186 2488 2869 1972
Size: 214 302 381 4025
Fragments arranged by size:
4025 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3214 3268 4752 4917 50
Size: 3164 54 1484 165 55
Fragments arranged by size:
3164 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1227 2834 1227
Size: 1607 3315
BsrFI r'CCGG_y
Cuts at: 2920 3101 2920
Size: 181 4741
BssSI C'ACGA_G
Cuts at: 519 3193 3901 519
Size: 2674 708 1540
Fragments arranged by size:
2674 1540 708
BstAPI GCAn_nnn'nTGC
Cuts at: 639 1893 2299 639
Size: 1254 406 3262
Fragments arranged by size:
3262 1254 406
BstBI TT'CG_AA
Cuts at: 1316 3283 1316
Size: 1967 2955
BstYI r'GATC_y
Cuts at: 1 195 512 1947 2414 2437 2772 3018 3324
Size: 194 317 1435 467 23 335 246 306
Cuts at: 3324 3336 3422 4474 1
Size: 12 86 1052 449
Fragments arranged by size:
1435 1052 467 449 335 317 306 246
194 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 463 463
Size: 4922
BtgI C'CryG_G
Cuts at: 107 107
Size: 4922
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2872 3053 3216 2872
Size: 181 163 4578
Fragments arranged by size:
4578 181 163
BtsI GCAGTG_nn'
Cuts at: 968 1239 2165 968
Size: 271 926 3725
Fragments arranged by size:
3725 926 271
DraI TTT'AAA
Cuts at: 569 1070 1921 569
Size: 501 851 3570
Fragments arranged by size:
3570 851 501
DrdI GACnn_nn'nnGTC
Cuts at: 2628 3972 2628
Size: 1344 3578
EaeI y'GGCC_r
Cuts at: 965 1271 2507 2681 3072 3099 4444 965
Size: 306 1236 174 391 27 1345 1443
Fragments arranged by size:
1443 1345 1236 391 306 174 27
EagI C'GGCC_G
Cuts at: 2507 2507
Size: 4922
EarI CTCTTCn'nnn_
Cuts at: 285 492 1204 2945 3155 285
Size: 207 712 1741 210 2052
Fragments arranged by size:
2052 1741 712 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3130 3872 4018 4732 3130
Size: 742 146 714 3320
Fragments arranged by size:
3320 742 714 146
EcoICRI GAG'CTC
Cuts at: 687 1785 1934 687
Size: 1098 149 3675
Fragments arranged by size:
3675 1098 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1187 1408 2747 3179 3532 4194 1187
Size: 221 1339 432 353 662 1915
Fragments arranged by size:
1915 1339 662 432 353 221
EcoO109I rG'GnC_Cy
Cuts at: 767 2061 767
Size: 1294 3628
EcoRI G'AATT_C
Cuts at: 779 1477 1926 779
Size: 698 449 3775
Fragments arranged by size:
3775 698 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 4922
FspI TGC'GCA
Cuts at: 2703 2703
Size: 4922
HaeII r_GCGC'y
Cuts at: 2356 2604 3834 4605 2356
Size: 248 1230 771 2673
Fragments arranged by size:
2673 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 170 174 202 284 316 2764 2794 2796
Size: 28 4 28 82 32 2448 30 2
Cuts at: 2796 2826 142
Size: 30 2238
Fragments arranged by size:
2448 2238 82 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 1961 1961
Size: 4922
HindIII A'AGCT_T
Cuts at: 1849 1849
Size: 4922
KasI G'GCGC_C
Cuts at: 2352 2600 2352
Size: 248 4674
KpnI G_GTAC'C
Cuts at: 1942 1942
Size: 4922
MluI A'CGCG_T
Cuts at: 527 4355 527
Size: 3828 1094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1265 3681 3865 1265
Size: 2416 184 2322
Fragments arranged by size:
2416 2322 184
MscI TGG'CCA
Cuts at: 967 1273 2683 967
Size: 306 1410 3206
Fragments arranged by size:
3206 1410 306
MslI CAynn'nnrTG
Cuts at: 1232 1279 1397 2911 3038 1232
Size: 47 118 1514 127 3116
Fragments arranged by size:
3116 1514 127 118 47
MspA1I CmG'CkG
Cuts at: 1260 2033 2347 2707 3491 3736 1260
Size: 773 314 360 784 245 2446
Fragments arranged by size:
2446 784 773 360 314 245
NaeI GCC'GGC
Cuts at: 3103 3103
Size: 4922
NarI GG'CG_CC
Cuts at: 2353 2601 2353
Size: 248 4674
NcoI C'CATG_G
Cuts at: 107 107
Size: 4922
NdeI CA'TA_TG
Cuts at: 262 262
Size: 4922
NgoMIV G'CCGG_C
Cuts at: 3101 3101
Size: 4922
NsiI A_TGCA'T
Cuts at: 1008 1008
Size: 4922
NspI r_CATG'y
Cuts at: 1975 2475 4078 1975
Size: 500 1603 2819
Fragments arranged by size:
2819 1603 500
PciI A'CATG_T
Cuts at: 4074 4074
Size: 4922
PfoI T'CCnGG_A
Cuts at: 222 222
Size: 4922
PmeI GTTT'AAAC
Cuts at: 1921 1921
Size: 4922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1817 1849 3368 3400 1817
Size: 32 1519 32 3339
Fragments arranged by size:
3339 1519 32 32
PpuMI rG'GwC_Cy
Cuts at: 767 767
Size: 4922
PsiI TTA'TAA
Cuts at: 1501 1501
Size: 4922
PstI C_TGCA'G
Cuts at: 1832 1969 2654 1832
Size: 137 685 4100
Fragments arranged by size:
4100 685 137
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4922
PvuII CAG'CTG
Cuts at: 1260 2347 2707 1260
Size: 1087 360 3475
Fragments arranged by size:
3475 1087 360
RsrII CG'GwC_CG
Cuts at: 3117 3117
Size: 4922
SacI G_AGCT'C
Cuts at: 689 1787 1936 689
Size: 1098 149 3675
Fragments arranged by size:
3675 1098 149
SalI G'TCGA_C
Cuts at: 1959 1959
Size: 4922
SapI GCTCTTCn'nnn_
Cuts at: 2945 3155 2945
Size: 210 4712
SbfI CC_TGCA'GG
Cuts at: 1969 1969
Size: 4922
ScaI AGT'ACT
Cuts at: 4439 4439
Size: 4922
SfcI C'TryA_G
Cuts at: 33 1296 1466 1828 1965 2650 3618 3809 33
Size: 1263 170 362 137 685 968 191 1146
Fragments arranged by size:
1263 1146 968 685 362 191 170 137
SfoI GGC'GCC
Cuts at: 2354 2602 2354
Size: 248 4674
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4922
SmaI CCC'GGG
Cuts at: 1944 1944
Size: 4922
SmlI C'TyrA_G
Cuts at: 176 378 1562 1819 2077 2992 3706 3968 176
Size: 202 1184 257 258 915 714 262 1130
Fragments arranged by size:
1184 1130 915 714 262 258 257 202
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4922
SphI G_CATG'C
Cuts at: 1975 2475 1975
Size: 500 4422
SspI AAT'ATT
Cuts at: 1047 1047
Size: 4922
StyI C'CwwG_G
Cuts at: 107 146 1268 2056 2410 107
Size: 39 1122 788 354 2619
Fragments arranged by size:
2619 1122 788 354 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 1698 2505 2638 3097 3306 358
Size: 1340 807 133 459 209 1974
Fragments arranged by size:
1974 1340 807 459 209 133
TatI w'GTAC_w
Cuts at: 4437 4437
Size: 4922
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4439 4471 4439
Size: 32 4890
Tth111I GACn'n_nGTC
Cuts at: 2719 2719
Size: 4922
XbaI T'CTAG_A
Cuts at: 432 1953 432
Size: 1521 3401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 791 1281 2417 791
Size: 490 1136 3296
Fragments arranged by size:
3296 1136 490
XhoI C'TCGA_G
Cuts at: 176 378 1562 176
Size: 202 1184 3536
Fragments arranged by size:
3536 1184 202
XmaI C'CCGG_G
Cuts at: 1942 1942
Size: 4922
ZraI GAC'GTC
Cuts at: 173 173
Size: 4922
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI