Restriction map for
pF1KB4122
(Circular) (Six-base) MAPSORT of: pF1KB4122.seq Check: 5299 from: 1 to: 4955
pF1KB4122 4955 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 700 1767 1993 700
Size: 1067 226 3662
Fragments arranged by size:
3662 1067 226
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1033 1507 2780 3212 3565 4227 998
Size: 35 474 1273 432 353 662 1726
Fragments arranged by size:
1726 1273 662 474 432 353 35
AflIII A'CryG_T
Cuts at: 2307 4107 4388 2307
Size: 1800 281 2874
Fragments arranged by size:
2874 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 683 683
Size: 4955
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1832 1864 1832
Size: 32 4923
AlwNI CAG_nnn'CTG
Cuts at: 2260 3698 2260
Size: 1438 3517
ApaI G_GGCC'C
Cuts at: 191 191
Size: 4955
ApaLI G'TGCA_C
Cuts at: 158 1016 3793 158
Size: 858 2777 1320
Fragments arranged by size:
2777 1320 858
ApoI r'AATT_y
Cuts at: 265 276 390 444 1264 1339 1959 4308 4564
Size: 11 114 54 820 75 620 2349 256
Cuts at: 4564 265
Size: 656
Fragments arranged by size:
2349 820 656 620 256 114 75 54
11
AseI AT'TA_AT
Cuts at: 20 1508 20
Size: 1488 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 635 1975 635
Size: 1340 3615
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 627 1971 2385 2633 2668 627
Size: 1344 414 248 35 2914
Fragments arranged by size:
2914 1344 414 248 35
BanII G_rGCy'C
Cuts at: 191 1969 2999 191
Size: 1778 1030 2147
Fragments arranged by size:
2147 1778 1030
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BbsI GAAGACnn'nnnn_
Cuts at: 792 1541 792
Size: 749 4206
BciVI GTATCCnnnnn_n'
Cuts at: 1228 2853 3909 4955 1228
Size: 1625 1056 1046 1228
Fragments arranged by size:
1625 1228 1056 1046
BclI T'GATC_A
Cuts at: 2475 2475
Size: 4955
BfrBI ATG'CAT
Cuts at: 1888 1888
Size: 4955
BglII A'GATC_T
Cuts at: 2470 4507 2470
Size: 2037 2918
BlpI GC'TnA_GC
Cuts at: 2067 2067
Size: 4955
Bme1580I G_kGCm'C
Cuts at: 162 191 1020 2580 2673 3797 162
Size: 29 829 1560 93 1124 1320
Fragments arranged by size:
1560 1320 1124 829 93 29
BmrI ACTGGGnnnn_n'
Cuts at: 1260 2167 2248 2583 4727 1260
Size: 907 81 335 2144 1488
Fragments arranged by size:
2144 1488 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 4955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1296 1296
Size: 4955
Bpu10I CC'TnA_GC
Cuts at: 473 473
Size: 4955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2131 3010 3724 4022 2131
Size: 879 714 298 3064
Fragments arranged by size:
3064 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 125 4659 125
Size: 4534 421
BsaAI yAC'GTr
Cuts at: 742 2308 742
Size: 1566 3389
BsaBI GATnn'nnATC
Cuts at: 6 2469 4506 6
Size: 2463 2037 455
Fragments arranged by size:
2463 2037 455
BsaHI Gr'CG_yC
Cuts at: 2386 2634 3336 4832 2386
Size: 248 702 1496 2509
Fragments arranged by size:
2509 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2144 2665 3343 3754 3901 4175 2144
Size: 521 678 411 147 274 2924
Fragments arranged by size:
2924 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1351 1381 1351
Size: 30 4925
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1206 1359 1580 1206
Size: 153 221 4581
Fragments arranged by size:
4581 221 153
BseYI C'CCAG_C
Cuts at: 2380 3138 3803 4211 2380
Size: 758 665 408 3124
Fragments arranged by size:
3124 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1892 676
Size: 1216 3739
BsiEI CG_ry'CG
Cuts at: 104 141 2497 2543 3773 104
Size: 37 2356 46 1230 1286
Fragments arranged by size:
2356 1286 1230 46 37
BsiHKAI G_wGCw'C
Cuts at: 162 293 723 1020 1969 2747 2937 3797 162
Size: 131 430 297 949 778 190 860 1320
Fragments arranged by size:
1320 949 860 778 430 297 190 131
BsmI GAATG_Cn'
Cuts at: 869 869
Size: 4955
Bsp1286I G_dGCh'C
Cuts at: 162 191 293 723 1020 1969 2580 2673 2747
Size: 29 102 430 297 949 611 93 74
Cuts at: 2747 2937 2999 3797 162
Size: 190 62 798 1320
Fragments arranged by size:
1320 949 798 611 430 297 190 102
93 74 62 29
BspHI T'CATG_A
Cuts at: 1068 1115 3387 1068
Size: 47 2272 2636
Fragments arranged by size:
2636 2272 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2005 2219 2521 2902 2005
Size: 214 302 381 4058
Fragments arranged by size:
4058 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3247 3301 4785 4950 50
Size: 3197 54 1484 165 55
Fragments arranged by size:
3197 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2867 2867
Size: 4955
BsrFI r'CCGG_y
Cuts at: 2953 3134 2953
Size: 181 4774
BssSI C'ACGA_G
Cuts at: 3226 3934 3226
Size: 708 4247
BstAPI GCAn_nnn'nTGC
Cuts at: 1894 2332 1894
Size: 438 4517
BstBI TT'CG_AA
Cuts at: 3316 3316
Size: 4955
BstYI r'GATC_y
Cuts at: 1 529 1980 2447 2470 2805 3051 3357 3369
Size: 528 1451 467 23 335 246 306 12
Cuts at: 3369 3455 4507 1
Size: 86 1052 449
Fragments arranged by size:
1451 1052 528 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1768 1768
Size: 4955
Bsu36I CC'TnA_GG
Cuts at: 725 725
Size: 4955
BtgI C'CryG_G
Cuts at: 135 1211 1221 135
Size: 1076 10 3869
Fragments arranged by size:
3869 1076 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 617 2905 3086 3249 617
Size: 2288 181 163 2323
Fragments arranged by size:
2323 2288 181 163
BtsI GCAGTG_nn'
Cuts at: 458 2198 458
Size: 1740 3215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1596 1631 1596
Size: 35 4920
DraI TTT'AAA
Cuts at: 1954 1954
Size: 4955
DrdI GACnn_nn'nnGTC
Cuts at: 2661 4005 2661
Size: 1344 3611
EaeI y'GGCC_r
Cuts at: 138 481 952 2540 2714 3105 3132 4477 138
Size: 343 471 1588 174 391 27 1345 616
Fragments arranged by size:
1588 1345 616 471 391 343 174 27
EagI C'GGCC_G
Cuts at: 138 2540 138
Size: 2402 2553
EarI CTCTTCn'nnn_
Cuts at: 1103 2978 3188 1103
Size: 1875 210 2870
Fragments arranged by size:
2870 1875 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 3905 4051 4765 3163
Size: 742 146 714 3353
Fragments arranged by size:
3353 742 714 146
EcoICRI GAG'CTC
Cuts at: 1967 1967
Size: 4955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1033 1296 1507 2780 3212 3565 4227 998
Size: 35 263 211 1273 432 353 662 1726
Fragments arranged by size:
1726 1273 662 432 353 263 211 35
EcoO109I rG'GnC_Cy
Cuts at: 187 188 2094 187
Size: 1 1906 3048
Fragments arranged by size:
3048 1906 1
EcoRI G'AATT_C
Cuts at: 1264 1959 1264
Size: 695 4260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4955
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 2389 2637 3867 4638 2389
Size: 248 1230 771 2706
Fragments arranged by size:
2706 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 891 923 1294 1326 1462 1494 1582 1614 1776
Size: 32 371 32 136 32 88 32 162
Cuts at: 1776 1808 1908 1940 2797 2827 2829 2859 891
Size: 32 100 32 857 30 2 30 2987
Fragments arranged by size:
2987 857 371 162 136 100 88 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1237 1994 1237
Size: 757 4198
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 1819 3714 3898 492
Size: 1327 1895 184 1549
Fragments arranged by size:
1895 1549 1327 184
MscI TGG'CCA
Cuts at: 483 954 2716 483
Size: 471 1762 2722
Fragments arranged by size:
2722 1762 471
MslI CAynn'nnrTG
Cuts at: 2944 3071 2944
Size: 127 4828
MspA1I CmG'CkG
Cuts at: 137 1906 2066 2380 2740 3524 3769 137
Size: 1769 160 314 360 784 245 1323
Fragments arranged by size:
1769 1323 784 360 314 245 160
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 4955
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NcoI C'CATG_G
Cuts at: 1211 1221 1211
Size: 10 4945
NdeI CA'TA_TG
Cuts at: 1884 1884
Size: 4955
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 4955
NsiI A_TGCA'T
Cuts at: 1890 1890
Size: 4955
NspI r_CATG'y
Cuts at: 689 2008 2508 4111 689
Size: 1319 500 1603 1533
Fragments arranged by size:
1603 1533 1319 500
PasI CC'CwG_GG
Cuts at: 217 217
Size: 4955
PciI A'CATG_T
Cuts at: 4107 4107
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 4923
PshAI GACnn'nnGTC
Cuts at: 1232 1232
Size: 4955
PspOMI G'GGCC_C
Cuts at: 187 187
Size: 4955
PstI C_TGCA'G
Cuts at: 1905 2002 2687 1905
Size: 97 685 4173
Fragments arranged by size:
4173 685 97
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 1906 2380 2740 1906
Size: 474 360 4121
Fragments arranged by size:
4121 474 360
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1969 1969
Size: 4955
SacII CC_GC'GG
Cuts at: 138 138
Size: 4955
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 2978 3188 2978
Size: 210 4745
SbfI CC_TGCA'GG
Cuts at: 2002 2002
Size: 4955
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SfcI C'TryA_G
Cuts at: 33 1298 1901 1998 2683 3651 3842 33
Size: 1265 603 97 685 968 191 1146
Fragments arranged by size:
1265 1146 968 685 603 191 97
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 1977 1977
Size: 4955
SmlI C'TyrA_G
Cuts at: 2110 3025 3739 4001 2110
Size: 915 714 262 3064
Fragments arranged by size:
3064 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4955
SphI G_CATG'C
Cuts at: 2008 2508 2008
Size: 500 4455
SspI AAT'ATT
Cuts at: 676 676
Size: 4955
StyI C'CwwG_G
Cuts at: 1211 1221 2089 2443 1211
Size: 10 868 354 3723
Fragments arranged by size:
3723 868 354 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 143 161 1190 2538 2671 3130 3339 143
Size: 18 1029 1348 133 459 209 1759
Fragments arranged by size:
1759 1348 1029 459 209 133 18
TatI w'GTAC_w
Cuts at: 4470 4470
Size: 4955
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 4923
Tth111I GACn'n_nGTC
Cuts at: 2752 2752
Size: 4955
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1146 2450 1146
Size: 1304 3651
XmaI C'CCGG_G
Cuts at: 1975 1975
Size: 4955
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstXI ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI