Restriction map for
pF1KB4123
(Circular) (Six-base) MAPSORT of: pF1KB4123.seq Check: 9245 from: 1 to: 4517
pF1KB4123 4517 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 989 1555 989
Size: 566 3951
Acc65I G'GTAC_C
Cuts at: 1319 1460 1533 1319
Size: 141 73 4303
Fragments arranged by size:
4303 141 73
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 2342 2774 3521 3789 182
Size: 2160 432 747 268 910
Fragments arranged by size:
2160 910 747 432 268
AflIII A'CryG_T
Cuts at: 767 1869 2973 3950 767
Size: 1102 1104 977 1334
Fragments arranged by size:
1334 1104 1102 977
AgeI A'CCGG_T
Cuts at: 2905 2905
Size: 4517
AleI CACnn'nnGTG
Cuts at: 673 786 788 673
Size: 113 2 4402
Fragments arranged by size:
4402 113 2
AlwNI CAG_nnn'CTG
Cuts at: 1822 3389 1822
Size: 1567 2950
ApaI G_GGCC'C
Cuts at: 1406 1406
Size: 4517
ApaLI G'TGCA_C
Cuts at: 3287 3287
Size: 4517
ApoI r'AATT_y
Cuts at: 1219 1521 3870 4126 1219
Size: 302 2349 256 1610
Fragments arranged by size:
2349 1610 302 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4517
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 245 302 1537 245
Size: 57 1235 3225
Fragments arranged by size:
3225 1235 57
BamHI G'GATC_C
Cuts at: 1542 1542
Size: 4517
BanI G'GyrC_C
Cuts at: 216 340 1319 1460 1533 1947 2195 2230 216
Size: 124 979 141 73 414 248 35 2503
Fragments arranged by size:
2503 979 414 248 141 124 73 35
BanII G_rGCy'C
Cuts at: 821 1161 1406 1531 2561 821
Size: 340 245 125 1030 2777
Fragments arranged by size:
2777 1030 340 245 125
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 751 1505 751
Size: 754 3763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1102 1136 1102
Size: 34 4483
BciVI GTATCCnnnnn_n'
Cuts at: 615 2415 3176 4517 615
Size: 1800 761 1341 615
Fragments arranged by size:
1800 1341 761 615
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BfrBI ATG'CAT
Cuts at: 998 998
Size: 4517
BglI GCCn_nnn'nGGC
Cuts at: 137 137
Size: 4517
BglII A'GATC_T
Cuts at: 683 2032 4069 683
Size: 1349 2037 1131
Fragments arranged by size:
2037 1349 1131
BlpI GC'TnA_GC
Cuts at: 616 1629 616
Size: 1013 3504
Bme1580I G_kGCm'C
Cuts at: 1406 2142 2235 3291 1406
Size: 736 93 1056 2632
Fragments arranged by size:
2632 1056 736 93
BmrI ACTGGGnnnn_n'
Cuts at: 1729 1810 2145 4289 1729
Size: 81 335 2144 1957
Fragments arranged by size:
2144 1957 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 1175 111
Size: 1064 3453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1693 2572 3064 3362 895
Size: 798 879 492 298 2050
Fragments arranged by size:
2050 879 798 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 969 4221 969
Size: 3252 1265
BsaAI yAC'GTr
Cuts at: 1870 1870
Size: 4517
BsaBI GATnn'nnATC
Cuts at: 6 1026 2031 4068 6
Size: 1020 1005 2037 455
Fragments arranged by size:
2037 1020 1005 455
BsaHI Gr'CG_yC
Cuts at: 1948 2196 2898 4394 1948
Size: 248 702 1496 2071
Fragments arranged by size:
2071 1496 702 248
BsaWI w'CCGG_w
Cuts at: 836 1316 1706 2227 2905 3179 3326 3737 836
Size: 480 390 521 678 274 147 411 1616
Fragments arranged by size:
1616 678 521 480 411 390 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 678 678
Size: 4517
BseYI C'CCAG_C
Cuts at: 1942 2700 3277 3773 1942
Size: 758 577 496 2686
Fragments arranged by size:
2686 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 1498
Size: 4517
BsiEI CG_ry'CG
Cuts at: 104 2059 2105 3313 104
Size: 1955 46 1208 1308
Fragments arranged by size:
1955 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 844 1531 2309 2499 3291 844
Size: 687 778 190 792 2070
Fragments arranged by size:
2070 792 778 687 190
BsmI GAATG_Cn'
Cuts at: 998 1380 998
Size: 382 4135
Bsp1286I G_dGCh'C
Cuts at: 821 844 1161 1406 1531 2142 2235 2309 2499
Size: 23 317 245 125 611 93 74 190
Cuts at: 2499 2561 3291 821
Size: 62 730 2047
Fragments arranged by size:
2047 730 611 317 245 190 125 93
74 62 23
BspEI T'CCGG_A
Cuts at: 3737 3737
Size: 4517
BspHI T'CATG_A
Cuts at: 477 1370 3693 477
Size: 893 2323 1301
Fragments arranged by size:
2323 1301 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1567 1781 2083 2464 611
Size: 956 214 302 381 2664
Fragments arranged by size:
2664 956 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2809 2863 4347 4512 50
Size: 2759 54 1484 165 55
Fragments arranged by size:
2759 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2429 2429
Size: 4517
BsrFI r'CCGG_y
Cuts at: 2515 2696 2905 2515
Size: 181 209 4127
Fragments arranged by size:
4127 209 181
BsrGI T'GTAC_A
Cuts at: 509 509
Size: 4517
BssSI C'ACGA_G
Cuts at: 2788 3146 2788
Size: 358 4159
BstAPI GCAn_nnn'nTGC
Cuts at: 1455 1894 1455
Size: 439 4078
BstBI TT'CG_AA
Cuts at: 699 2878 699
Size: 2179 2338
BstYI r'GATC_y
Cuts at: 1 683 1542 2009 2032 2367 2613 3625 3711
Size: 682 859 467 23 335 246 1012 86
Cuts at: 3711 3723 4069 1
Size: 12 346 449
Fragments arranged by size:
1012 859 682 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1424 1424
Size: 4517
BtgI C'CryG_G
Cuts at: 107 980 107
Size: 873 3644
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 807 2467 2648 2811 807
Size: 1660 181 163 2513
Fragments arranged by size:
2513 1660 181 163
BtsI GCAGTG_nn'
Cuts at: 1760 1760
Size: 4517
DraI TTT'AAA
Cuts at: 1516 1516
Size: 4517
DraIII CAC_nnn'GTG
Cuts at: 450 450
Size: 4517
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3081 2223
Size: 858 3659
EaeI y'GGCC_r
Cuts at: 2102 2276 2667 2694 4039 2102
Size: 174 391 27 1345 2580
Fragments arranged by size:
2580 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2102 2102
Size: 4517
EarI CTCTTCn'nnn_
Cuts at: 273 433 454 2540 2750 273
Size: 160 21 2086 210 2040
Fragments arranged by size:
2086 2040 210 160 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 328 2725 3035 3181 4327 328
Size: 2397 310 146 1146 518
Fragments arranged by size:
2397 1146 518 310 146
EcoICRI GAG'CTC
Cuts at: 1529 1529
Size: 4517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 182 1175 2342 2774 3521 3789 111
Size: 71 993 1167 432 747 268 839
Fragments arranged by size:
1167 993 839 747 432 268 71
EcoNI CCTnn'n_nnAGG
Cuts at: 1339 1339
Size: 4517
EcoO109I rG'GnC_Cy
Cuts at: 1418 1656 1418
Size: 238 4279
EcoRI G'AATT_C
Cuts at: 1521 1521
Size: 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4517
FspI TGC'GCA
Cuts at: 2298 2298
Size: 4517
HaeII r_GCGC'y
Cuts at: 1951 2199 3221 4200 1951
Size: 248 1022 979 2268
Fragments arranged by size:
2268 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 465 497 2359 2389 2391 2421 285
Size: 32 148 32 1862 30 2 30 2381
Fragments arranged by size:
2381 1862 148 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 1323 1464 1537 1323
Size: 141 73 4303
Fragments arranged by size:
4303 141 73
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3188 3372 3188
Size: 184 4333
MscI TGG'CCA
Cuts at: 2278 2278
Size: 4517
MslI CAynn'nnrTG
Cuts at: 673 786 788 1411 2506 2633 673
Size: 113 2 623 1095 127 2557
Fragments arranged by size:
2557 1095 623 127 113 2
MspA1I CmG'CkG
Cuts at: 366 673 1628 1942 2302 3315 3560 366
Size: 307 955 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 955 360 314 307 245
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 4517
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 107 980 107
Size: 873 3644
NdeI CA'TA_TG
Cuts at: 994 1203 994
Size: 209 4308
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 4517
NsiI A_TGCA'T
Cuts at: 1000 1000
Size: 4517
NspI r_CATG'y
Cuts at: 1570 2070 2977 1570
Size: 500 907 3110
Fragments arranged by size:
3110 907 500
PciI A'CATG_T
Cuts at: 2973 2973
Size: 4517
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4485
PspOMI G'GGCC_C
Cuts at: 1402 1402
Size: 4517
PstI C_TGCA'G
Cuts at: 1564 2249 1564
Size: 685 3832
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 1942 2302 1942
Size: 360 4157
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 1531 1531
Size: 4517
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 433 2540 2750 433
Size: 2107 210 2200
Fragments arranged by size:
2200 2107 210
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SfcI C'TryA_G
Cuts at: 33 420 1560 2245 3238 3429 33
Size: 387 1140 685 993 191 1121
Fragments arranged by size:
1140 1121 993 685 387 191
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 1539 1539
Size: 4517
SmlI C'TyrA_G
Cuts at: 910 1672 2587 3079 3341 910
Size: 762 915 492 262 2086
Fragments arranged by size:
2086 915 762 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1570 2070 1570
Size: 500 4017
SspI AAT'ATT
Cuts at: 271 796 271
Size: 525 3992
StuI AGG'CCT
Cuts at: 1037 1037
Size: 4517
StyI C'CwwG_G
Cuts at: 107 828 980 1651 2005 107
Size: 721 152 671 354 2619
Fragments arranged by size:
2619 721 671 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 442 2100 2233 2692 2901 442
Size: 1658 133 459 209 2058
Fragments arranged by size:
2058 1658 459 209 133
TatI w'GTAC_w
Cuts at: 509 533 929 4032 509
Size: 24 396 3103 994
Fragments arranged by size:
3103 994 396 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4034 4066 4034
Size: 32 4485
Tth111I GACn'n_nGTC
Cuts at: 2314 2314
Size: 4517
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2012 2012
Size: 4517
XmaI C'CCGG_G
Cuts at: 1537 1537
Size: 4517
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI