Restriction map for
pF1KB4125
(Circular) (Six-base) MAPSORT of: pF1KB4125.seq Check: 788 from: 1 to: 6263
pF1KB4125 6263 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1008 1008
Size: 6263
AccI GT'mk_AC
Cuts at: 3301 3301
Size: 6263
Acc65I G'GTAC_C
Cuts at: 3279 3279
Size: 6263
AclI AA'CG_TT
Cuts at: 6103 6103
Size: 6263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 2810 3014 4088 4520 5267 5535 820
Size: 1990 204 1074 432 747 268 1548
Fragments arranged by size:
1990 1548 1074 747 432 268 204
AfeI AGC'GCT
Cuts at: 346 367 346
Size: 21 6242
AflIII A'CryG_T
Cuts at: 664 817 832 3615 4719 5696 664
Size: 153 15 2783 1104 977 1231
Fragments arranged by size:
2783 1231 1104 977 153 15
AgeI A'CCGG_T
Cuts at: 1259 4651 1259
Size: 3392 2871
AlwNI CAG_nnn'CTG
Cuts at: 1576 2125 2224 3568 5135 1576
Size: 549 99 1344 1567 2704
Fragments arranged by size:
2704 1567 1344 549 99
ApaI G_GGCC'C
Cuts at: 176 223 1610 176
Size: 47 1387 4829
Fragments arranged by size:
4829 1387 47
ApaLI G'TGCA_C
Cuts at: 5033 5033
Size: 6263
ApoI r'AATT_y
Cuts at: 961 1437 2091 2682 3040 3148 3175 3267 5616
Size: 476 654 591 358 108 27 92 2349
Cuts at: 5616 5872 961
Size: 256 1352
Fragments arranged by size:
2349 1352 654 591 476 358 256 108
92 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 6263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
AvaI C'yCGr_G
Cuts at: 3283 3283
Size: 6263
BamHI G'GATC_C
Cuts at: 2523 3288 2523
Size: 765 5498
BanI G'GyrC_C
Cuts at: 183 306 1268 3279 3693 3941 3976 183
Size: 123 962 2011 414 248 35 2470
Fragments arranged by size:
2470 2011 962 414 248 123 35
BanII G_rGCy'C
Cuts at: 176 223 400 678 1350 1610 1945 2419 3277
Size: 47 177 278 672 260 335 474 858
Cuts at: 3277 4307 176
Size: 1030 2132
Fragments arranged by size:
2132 1030 858 672 474 335 278 260
177 47
BbeI G_GCGC'C
Cuts at: 187 310 3697 3945 187
Size: 123 3387 248 2505
Fragments arranged by size:
3387 2505 248 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 134 168 1258 1292 134
Size: 34 1090 34 5105
Fragments arranged by size:
5105 1090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4161 4922 6263 4161
Size: 761 1341 4161
Fragments arranged by size:
4161 1341 761
BclI T'GATC_A
Cuts at: 911 3783 911
Size: 2872 3391
BfrBI ATG'CAT
Cuts at: 1188 1294 1188
Size: 106 6157
BglII A'GATC_T
Cuts at: 3778 5815 3778
Size: 2037 4226
BlpI GC'TnA_GC
Cuts at: 3375 3375
Size: 6263
Bme1580I G_kGCm'C
Cuts at: 176 223 1610 2641 2672 3888 3981 5037 176
Size: 47 1387 1031 31 1216 93 1056 1402
Fragments arranged by size:
1402 1387 1216 1056 1031 93 47 31
BmgBI CAC'GTC
Cuts at: 665 665
Size: 6263
BmrI ACTGGGnnnn_n'
Cuts at: 484 1294 2239 2756 3475 3556 3891 6035 484
Size: 810 945 517 719 81 335 2144 712
Fragments arranged by size:
2144 945 810 719 712 517 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 4630 2635
Size: 1995 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 1501 2093 2157 2278 719
Size: 782 592 64 121 4704
Fragments arranged by size:
4704 782 592 121 64
Bpu10I CC'TnA_GC
Cuts at: 1412 1412
Size: 6263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3439 4318 4810 5108 3439
Size: 879 492 298 4594
Fragments arranged by size:
4594 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 400 5967 400
Size: 5567 696
BsaAI yAC'GTr
Cuts at: 3616 3616
Size: 6263
BsaBI GATnn'nnATC
Cuts at: 6 3777 5814 6
Size: 3771 2037 455
Fragments arranged by size:
3771 2037 455
BsaHI Gr'CG_yC
Cuts at: 184 307 3694 3942 4644 6140 184
Size: 123 3387 248 702 1496 307
Fragments arranged by size:
3387 1496 702 307 248 123
BsaWI w'CCGG_w
Cuts at: 1259 1980 2295 3452 3973 4651 4925 5072 5483
Size: 721 315 1157 521 678 274 147 411
Cuts at: 5483 1259
Size: 2039
Fragments arranged by size:
2039 1157 721 678 521 411 315 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1256 1286 1256
Size: 30 6233
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1694 2443 2611 1694
Size: 749 168 5346
Fragments arranged by size:
5346 749 168
BseYI C'CCAG_C
Cuts at: 362 393 562 3688 4446 5023 5519 362
Size: 31 169 3126 758 577 496 1106
Fragments arranged by size:
3126 1106 758 577 496 169 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 530 1913 2615 313
Size: 217 1383 702 3961
Fragments arranged by size:
3961 1383 702 217
BsiEI CG_ry'CG
Cuts at: 104 404 3805 3851 5059 104
Size: 300 3401 46 1208 1308
Fragments arranged by size:
3401 1308 1208 300 46
BsiHKAI G_wGCw'C
Cuts at: 678 1945 2419 3277 4055 4245 5037 678
Size: 1267 474 858 778 190 792 1904
Fragments arranged by size:
1904 1267 858 792 778 474 190
BsmI GAATG_Cn'
Cuts at: 1604 1604
Size: 6263
Bsp1286I G_dGCh'C
Cuts at: 176 223 400 678 1350 1610 1945 2419 2641
Size: 47 177 278 672 260 335 474 222
Cuts at: 2641 2672 3277 3888 3981 4055 4245 4307 5037
Size: 31 605 611 93 74 190 62 730
Cuts at: 5037 176
Size: 1402
Fragments arranged by size:
1402 730 672 611 605 474 335 278
260 222 190 177 93 74 62 47
31
BspEI T'CCGG_A
Cuts at: 1980 5483 1980
Size: 3503 2760
BspHI T'CATG_A
Cuts at: 5439 5439
Size: 6263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1008 3313 3527 3829 4210 1008
Size: 2305 214 302 381 3061
Fragments arranged by size:
3061 2305 381 302 214
BsrBI CCG'CTC
Cuts at: 50 191 4555 4609 6093 6258 50
Size: 141 4364 54 1484 165 55
Fragments arranged by size:
4364 1484 165 141 55 54
BsrDI GCAATG_nn'
Cuts at: 4175 4175
Size: 6263
BsrFI r'CCGG_y
Cuts at: 438 1259 4261 4442 4651 438
Size: 821 3002 181 209 2050
Fragments arranged by size:
3002 2050 821 209 181
BsrGI T'GTAC_A
Cuts at: 595 2142 3204 595
Size: 1547 1062 3654
Fragments arranged by size:
3654 1547 1062
BssSI C'ACGA_G
Cuts at: 4534 4892 4534
Size: 358 5905
BstAPI GCAn_nnn'nTGC
Cuts at: 1185 1557 2714 3640 1185
Size: 372 1157 926 3808
Fragments arranged by size:
3808 1157 926 372
BstBI TT'CG_AA
Cuts at: 2268 4624 2268
Size: 2356 3907
BstXI CCAn_nnnn'nTGG
Cuts at: 114 939 114
Size: 825 5438
BstYI r'GATC_y
Cuts at: 1 977 2523 3288 3755 3778 4113 4359 5371
Size: 976 1546 765 467 23 335 246 1012
Cuts at: 5371 5457 5469 5815 1
Size: 86 12 346 449
Fragments arranged by size:
1546 1012 976 765 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1105 4213 4394 4557 1105
Size: 3108 181 163 2811
Fragments arranged by size:
3108 2811 181 163
BtsI GCAGTG_nn'
Cuts at: 1830 3506 1830
Size: 1676 4587
ClaI AT'CG_AT
Cuts at: 1934 1934
Size: 6263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 453 488 453
Size: 35 6228
DraI TTT'AAA
Cuts at: 3262 3262
Size: 6263
DraIII CAC_nnn'GTG
Cuts at: 1453 2167 1453
Size: 714 5549
DrdI GACnn_nn'nnGTC
Cuts at: 709 3969 4827 709
Size: 3260 858 2145
Fragments arranged by size:
3260 2145 858
EaeI y'GGCC_r
Cuts at: 401 2049 3848 4022 4413 4440 5785 401
Size: 1648 1799 174 391 27 1345 879
Fragments arranged by size:
1799 1648 1345 879 391 174 27
EagI C'GGCC_G
Cuts at: 401 3848 401
Size: 3447 2816
EarI CTCTTCn'nnn_
Cuts at: 1982 2208 2549 4286 4496 1982
Size: 226 341 1737 210 3749
Fragments arranged by size:
3749 1737 341 226 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4471 4781 4927 6073 4471
Size: 310 146 1146 4661
Fragments arranged by size:
4661 1146 310 146
EcoICRI GAG'CTC
Cuts at: 676 1943 2417 3275 676
Size: 1267 474 858 3664
Fragments arranged by size:
3664 1267 858 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 820 1501 2093 2157 2278 2810 3014 4088
Size: 101 681 592 64 121 532 204 1074
Cuts at: 4088 4520 5267 5535 719
Size: 432 747 268 1447
Fragments arranged by size:
1447 1074 747 681 592 532 432 268
204 121 101 64
EcoNI CCTnn'n_nnAGG
Cuts at: 1111 1111
Size: 6263
EcoO109I rG'GnC_Cy
Cuts at: 172 173 219 1606 2122 3402 172
Size: 1 46 1387 516 1280 3033
Fragments arranged by size:
3033 1387 1280 516 46 1
EcoRI G'AATT_C
Cuts at: 1437 3040 3148 3267 1437
Size: 1603 108 119 4433
Fragments arranged by size:
4433 1603 119 108
EcoRV GAT'ATC
Cuts at: 1726 1726
Size: 6263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4282 4282
Size: 6263
FspI TGC'GCA
Cuts at: 4044 4044
Size: 6263
HaeII r_GCGC'y
Cuts at: 187 310 348 369 2838 3697 3945 4967 5946
Size: 123 38 21 2469 859 248 1022 979
Cuts at: 5946 187
Size: 504
Fragments arranged by size:
2469 1022 979 859 504 248 123 38
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 1927 1959 2332 2364 2482 2514 4105
Size: 32 1180 32 373 32 118 32 1591
Cuts at: 4105 4135 4137 4167 715
Size: 30 2 30 2811
Fragments arranged by size:
2811 1591 1180 373 118 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2807 2945 3302 2807
Size: 138 357 5768
Fragments arranged by size:
5768 357 138
HindIII A'AGCT_T
Cuts at: 890 890
Size: 6263
HpaI GTT'AAC
Cuts at: 2807 2807
Size: 6263
KasI G'GCGC_C
Cuts at: 183 306 3693 3941 183
Size: 123 3387 248 2505
Fragments arranged by size:
3387 2505 248 123
KpnI G_GTAC'C
Cuts at: 3283 3283
Size: 6263
MfeI C'AATT_G
Cuts at: 1171 1171
Size: 6263
MluI A'CGCG_T
Cuts at: 5696 5696
Size: 6263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 245 453 2160 2501 2532 4934 5118 245
Size: 208 1707 341 31 2402 184 1390
Fragments arranged by size:
2402 1707 1390 341 208 184 31
MscI TGG'CCA
Cuts at: 2051 4024 2051
Size: 1973 4290
MslI CAynn'nnrTG
Cuts at: 112 4252 4379 112
Size: 4140 127 1996
Fragments arranged by size:
4140 1996 127
MspA1I CmG'CkG
Cuts at: 148 446 798 3374 3688 4048 5061 5306 148
Size: 298 352 2576 314 360 1013 245 1105
Fragments arranged by size:
2576 1105 1013 360 352 314 298 245
NaeI GCC'GGC
Cuts at: 4444 4444
Size: 6263
NarI GG'CG_CC
Cuts at: 184 307 3694 3942 184
Size: 123 3387 248 2505
Fragments arranged by size:
3387 2505 248 123
NcoI C'CATG_G
Cuts at: 107 107
Size: 6263
NdeI CA'TA_TG
Cuts at: 1523 1523
Size: 6263
NgoMIV G'CCGG_C
Cuts at: 4442 4442
Size: 6263
NsiI A_TGCA'T
Cuts at: 1190 1296 1190
Size: 106 6157
NspI r_CATG'y
Cuts at: 821 836 1183 3316 3816 4723 821
Size: 15 347 2133 500 907 2361
Fragments arranged by size:
2361 2133 907 500 347 15
PciI A'CATG_T
Cuts at: 817 832 4719 817
Size: 15 3887 2361
Fragments arranged by size:
3887 2361 15
PflMI CCAn_nnn'nTGG
Cuts at: 1453 1453
Size: 6263
PmeI GTTT'AAAC
Cuts at: 3262 3262
Size: 6263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5402 5434 5402
Size: 32 6231
PpuMI rG'GwC_Cy
Cuts at: 2122 2122
Size: 6263
PsiI TTA'TAA
Cuts at: 1964 1964
Size: 6263
PspOMI G'GGCC_C
Cuts at: 172 219 1606 172
Size: 47 1387 4829
Fragments arranged by size:
4829 1387 47
PstI C_TGCA'G
Cuts at: 3310 3995 3310
Size: 685 5578
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6263
PvuII CAG'CTG
Cuts at: 798 3688 4048 798
Size: 2890 360 3013
Fragments arranged by size:
3013 2890 360
RsrII CG'GwC_CG
Cuts at: 4458 4458
Size: 6263
SacI G_AGCT'C
Cuts at: 678 1945 2419 3277 678
Size: 1267 474 858 3664
Fragments arranged by size:
3664 1267 858 474
SalI G'TCGA_C
Cuts at: 3300 3300
Size: 6263
SapI GCTCTTCn'nnn_
Cuts at: 2208 4286 4496 2208
Size: 2078 210 3975
Fragments arranged by size:
3975 2078 210
SbfI CC_TGCA'GG
Cuts at: 3310 3310
Size: 6263
ScaI AGT'ACT
Cuts at: 2001 5780 2001
Size: 3779 2484
SfcI C'TryA_G
Cuts at: 33 1774 2813 3306 3991 4984 5175 33
Size: 1741 1039 493 685 993 191 1121
Fragments arranged by size:
1741 1121 1039 993 685 493 191
SfoI GGC'GCC
Cuts at: 185 308 3695 3943 185
Size: 123 3387 248 2505
Fragments arranged by size:
3387 2505 248 123
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
SmaI CCC'GGG
Cuts at: 3285 3285
Size: 6263
SmlI C'TyrA_G
Cuts at: 3418 4333 4825 5087 3418
Size: 915 492 262 4594
Fragments arranged by size:
4594 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1888 2137 67
Size: 1821 249 4193
Fragments arranged by size:
4193 1821 249
SphI G_CATG'C
Cuts at: 3316 3816 3316
Size: 500 5763
SspI AAT'ATT
Cuts at: 2019 2019
Size: 6263
StuI AGG'CCT
Cuts at: 2114 2355 2795 2114
Size: 241 440 5582
Fragments arranged by size:
5582 440 241
StyI C'CwwG_G
Cuts at: 107 3397 3751 107
Size: 3290 354 2619
Fragments arranged by size:
3290 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 481 3846 3979 4438 4647 481
Size: 3365 133 459 209 2097
Fragments arranged by size:
3365 2097 459 209 133
TatI w'GTAC_w
Cuts at: 568 595 806 1222 1999 2142 2461 2981 3204
Size: 27 211 416 777 143 319 520 223
Cuts at: 3204 5778 568
Size: 2574 1053
Fragments arranged by size:
2574 1053 777 520 416 319 223 211
143 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 5780 5812 2225
Size: 32 3523 32 2676
Fragments arranged by size:
3523 2676 32 32
Tth111I GACn'n_nGTC
Cuts at: 4060 4060
Size: 6263
XbaI T'CTAG_A
Cuts at: 3294 3294
Size: 6263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2696 3758 2696
Size: 1062 5201
XmaI C'CCGG_G
Cuts at: 3283 3283
Size: 6263
XmnI GAAnn'nnTTC
Cuts at: 893 3104 893
Size: 2211 4052
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BglI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I FseI FspAI NheI NotI NruI
PacI PasI PfoI PmlI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI