Restriction map for
pF1KB4126
(Circular) (Six-base) MAPSORT of: pF1KB4126.seq Check: 4672 from: 1 to: 4412
pF1KB4126 4412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1450 1450
Size: 4412
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 503 4252 503
Size: 3749 663
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 950 1026 2237 2669 3022 3684 796
Size: 154 76 1211 432 353 662 1524
Fragments arranged by size:
1524 1211 662 432 353 154 76
AflIII A'CryG_T
Cuts at: 917 1764 3564 3845 917
Size: 847 1800 281 1484
Fragments arranged by size:
1800 1484 847 281
AlwNI CAG_nnn'CTG
Cuts at: 973 1717 3155 973
Size: 744 1438 2230
Fragments arranged by size:
2230 1438 744
ApaLI G'TGCA_C
Cuts at: 3250 3250
Size: 4412
ApoI r'AATT_y
Cuts at: 557 645 1225 1416 3765 4021 557
Size: 88 580 191 2349 256 948
Fragments arranged by size:
2349 948 580 256 191 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 1432 1432
Size: 4412
BamHI G'GATC_C
Cuts at: 1437 1437
Size: 4412
BanI G'GyrC_C
Cuts at: 1428 1842 2090 2125 1428
Size: 414 248 35 3715
Fragments arranged by size:
3715 414 248 35
BanII G_rGCy'C
Cuts at: 1426 2456 1426
Size: 1030 3382
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbsI GAAGACnn'nnnn_
Cuts at: 649 1032 649
Size: 383 4029
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3366 4412 2310
Size: 1056 1046 2310
Fragments arranged by size:
2310 1056 1046
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BfrBI ATG'CAT
Cuts at: 1210 1210
Size: 4412
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 1524 1524
Size: 4412
Bme1580I G_kGCm'C
Cuts at: 2037 2130 3254 2037
Size: 93 1124 3195
Fragments arranged by size:
3195 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 736 1624 1705 2040 4184 736
Size: 888 81 335 2144 964
Fragments arranged by size:
2144 964 888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 448
Size: 4412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 2467 3181 3479 1588
Size: 879 714 298 2521
Fragments arranged by size:
2521 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4116 4116
Size: 4412
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 241 1601 2122 2800 3211 3358 3632 241
Size: 1360 521 678 411 147 274 1021
Fragments arranged by size:
1360 1021 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1837 2595 3260 3668 1837
Size: 758 665 408 2581
Fragments arranged by size:
2581 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1954 2000 3230 104
Size: 1850 46 1230 1286
Fragments arranged by size:
1850 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1426 2204 2394 3254 1426
Size: 778 190 860 2584
Fragments arranged by size:
2584 860 778 190
BsmI GAATG_Cn'
Cuts at: 1210 1210
Size: 4412
Bsp1286I G_dGCh'C
Cuts at: 1426 2037 2130 2204 2394 2456 3254 1426
Size: 611 93 74 190 62 798 2584
Fragments arranged by size:
2584 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 555 1462 1676 1978 2359 555
Size: 907 214 302 381 2608
Fragments arranged by size:
2608 907 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2704 2758 4242 4407 50
Size: 2654 54 1484 165 55
Fragments arranged by size:
2654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2324 2324
Size: 4412
BsrFI r'CCGG_y
Cuts at: 2410 2591 2410
Size: 181 4231
BssSI C'ACGA_G
Cuts at: 2683 3391 2683
Size: 708 3704
BstAPI GCAn_nnn'nTGC
Cuts at: 1789 1789
Size: 4412
BstBI TT'CG_AA
Cuts at: 828 2773 828
Size: 1945 2467
BstYI r'GATC_y
Cuts at: 1 755 1326 1437 1904 1927 2262 2508 2814
Size: 754 571 111 467 23 335 246 306
Cuts at: 2814 2826 2912 3964 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 754 571 467 449 335 306 246
111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1391 1391
Size: 4412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 437 936 1655 437
Size: 499 719 3194
Fragments arranged by size:
3194 719 499
DraI TTT'AAA
Cuts at: 1103 1411 1103
Size: 308 4104
DrdI GACnn_nn'nnGTC
Cuts at: 2118 3462 2118
Size: 1344 3068
EaeI y'GGCC_r
Cuts at: 1997 2171 2562 2589 3934 1997
Size: 174 391 27 1345 2475
Fragments arranged by size:
2475 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2620 3362 3508 4222 2620
Size: 742 146 714 2810
Fragments arranged by size:
2810 742 714 146
EcoICRI GAG'CTC
Cuts at: 1424 1424
Size: 4412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 796 950 1026 2237 2669 3022 3684 448
Size: 348 154 76 1211 432 353 662 1176
Fragments arranged by size:
1211 1176 662 432 353 348 154 76
EcoO109I rG'GnC_Cy
Cuts at: 1551 1551
Size: 4412
EcoRI G'AATT_C
Cuts at: 557 1416 557
Size: 859 3553
EcoRV GAT'ATC
Cuts at: 593 593
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1056 2431 1056
Size: 1375 3037
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1846 2094 3324 4095 1846
Size: 248 1230 771 2163
Fragments arranged by size:
2163 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 518 550 873 905 2254 2284 2286 2316 518
Size: 32 323 32 1349 30 2 30 2614
Fragments arranged by size:
2614 1349 323 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
HindIII A'AGCT_T
Cuts at: 222 222
Size: 4412
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 341 3171 3355 341
Size: 2830 184 1398
Fragments arranged by size:
2830 1398 184
MscI TGG'CCA
Cuts at: 2173 2173
Size: 4412
MslI CAynn'nnrTG
Cuts at: 160 193 693 2401 2528 160
Size: 33 500 1708 127 2044
Fragments arranged by size:
2044 1708 500 127 33
MspA1I CmG'CkG
Cuts at: 1523 1837 2197 2981 3226 1523
Size: 314 360 784 245 2709
Fragments arranged by size:
2709 784 360 314 245
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NsiI A_TGCA'T
Cuts at: 1212 1212
Size: 4412
NspI r_CATG'y
Cuts at: 141 921 1465 1965 3568 141
Size: 780 544 500 1603 985
Fragments arranged by size:
1603 985 780 544 500
PciI A'CATG_T
Cuts at: 917 3564 917
Size: 2647 1765
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 4380
PstI C_TGCA'G
Cuts at: 1459 2144 1459
Size: 685 3727
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 1837 2197 1837
Size: 360 4052
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 1426 1426
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 3929 3929
Size: 4412
SfcI C'TryA_G
Cuts at: 33 404 911 1271 1455 2140 3108 3299 33
Size: 371 507 360 184 685 968 191 1146
Fragments arranged by size:
1146 968 685 507 371 360 191 184
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 1434 1434
Size: 4412
SmlI C'TyrA_G
Cuts at: 1567 2482 3196 3458 1567
Size: 915 714 262 2521
Fragments arranged by size:
2521 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
SspI AAT'ATT
Cuts at: 764 825 764
Size: 61 4351
StyI C'CwwG_G
Cuts at: 1546 1900 1546
Size: 354 4058
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 363 703 750 3927 363
Size: 340 47 3177 848
Fragments arranged by size:
3177 848 340 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 4380
Tth111I GACn'n_nGTC
Cuts at: 2209 2209
Size: 4412
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1907 1907
Size: 4412
XmaI C'CCGG_G
Cuts at: 1432 1432
Size: 4412
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI