Restriction map for
pF1KB4140
(Circular) (Six-base) MAPSORT of: pF1KB4140.seq Check: 4939 from: 1 to: 5354
pF1KB4140 5354 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1564 2193 1564
Size: 629 4725
AatII G_ACGT'C
Cuts at: 368 368
Size: 5354
AccI GT'mk_AC
Cuts at: 1376 1920 2144 2392 1376
Size: 544 224 248 4338
Fragments arranged by size:
4338 544 248 224
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1975 2341 3179 3611 3964 4626 1975
Size: 366 838 432 353 662 2703
Fragments arranged by size:
2703 838 662 432 366 353
AflIII A'CryG_T
Cuts at: 584 1890 2706 4506 4787 584
Size: 1306 816 1800 281 1151
Fragments arranged by size:
1800 1306 1151 816 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1502 1502
Size: 5354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1582 1614 1582
Size: 32 5322
AlwNI CAG_nnn'CTG
Cuts at: 287 523 543 1086 2053 2199 2208 2243 2659
Size: 236 20 543 967 146 9 35 416
Cuts at: 2659 4097 287
Size: 1438 1544
Fragments arranged by size:
1544 1438 967 543 416 236 146 35
20 9
ApaI G_GGCC'C
Cuts at: 2101 2319 2101
Size: 218 5136
ApaLI G'TGCA_C
Cuts at: 1435 4192 1435
Size: 2757 2597
ApoI r'AATT_y
Cuts at: 1202 1830 2358 4707 4963 1202
Size: 628 528 2349 256 1593
Fragments arranged by size:
2349 1593 628 528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5354
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 848 2171 2374 848
Size: 1323 203 3828
Fragments arranged by size:
3828 1323 203
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 1249 1576 2370 2784 3032 3067 1249
Size: 327 794 414 248 35 3536
Fragments arranged by size:
3536 794 414 327 248 35
BanII G_rGCy'C
Cuts at: 277 914 1812 2101 2255 2319 2368 3398 277
Size: 637 898 289 154 64 49 1030 2233
Fragments arranged by size:
2233 1030 898 637 289 154 64 49
BbeI G_GCGC'C
Cuts at: 2788 3036 2788
Size: 248 5106
BbsI GAAGACnn'nnnn_
Cuts at: 368 368
Size: 5354
BbvCI CC'TCA_GC
Cuts at: 413 1901 413
Size: 1488 3866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 315 349 1345 1379 315
Size: 34 996 34 4290
Fragments arranged by size:
4290 996 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3252 4308 5354 3252
Size: 1056 1046 3252
Fragments arranged by size:
3252 1056 1046
BclI T'GATC_A
Cuts at: 2874 2874
Size: 5354
BglI GCCn_nnn'nGGC
Cuts at: 2019 2096 2333 2019
Size: 77 237 5040
Fragments arranged by size:
5040 237 77
BglII A'GATC_T
Cuts at: 1001 2869 4906 1001
Size: 1868 2037 1449
Fragments arranged by size:
2037 1868 1449
BlpI GC'TnA_GC
Cuts at: 2466 2466
Size: 5354
Bme1580I G_kGCm'C
Cuts at: 1439 1581 1667 2101 2319 2979 3072 4196 1439
Size: 142 86 434 218 660 93 1124 2597
Fragments arranged by size:
2597 1124 660 434 218 142 93 86
BmgBI CAC'GTC
Cuts at: 344 534 1677 344
Size: 190 1143 4021
Fragments arranged by size:
4021 1143 190
BmrI ACTGGGnnnn_n'
Cuts at: 1590 2566 2647 2982 5126 1590
Size: 976 81 335 2144 1818
Fragments arranged by size:
2144 1818 976 335 81
BmtI G_CTAG'C
Cuts at: 1469 1469
Size: 5354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 589 631 646 1834 2185 396
Size: 193 42 15 1188 351 3565
Fragments arranged by size:
3565 1188 351 193 42 15
Bpu10I CC'TnA_GC
Cuts at: 413 501 1901 1973 413
Size: 88 1400 72 3794
Fragments arranged by size:
3794 1400 88 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1684 2052 2530 3409 4123 4421 1165
Size: 519 368 478 879 714 298 2098
Fragments arranged by size:
2098 879 714 519 478 368 298
BsaI GGTCTCn'nnnn_
Cuts at: 412 5058 412
Size: 4646 708
BsaAI yAC'GTr
Cuts at: 587 1434 2707 587
Size: 847 1273 3234
Fragments arranged by size:
3234 1273 847
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 365 563 2785 3033 3735 5231 365
Size: 198 2222 248 702 1496 488
Fragments arranged by size:
2222 1496 702 488 248 198
BsaWI w'CCGG_w
Cuts at: 830 2543 3064 3742 4153 4300 4574 830
Size: 1713 521 678 411 147 274 1610
Fragments arranged by size:
1713 1610 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 112
Size: 30 5324
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 430 643 126
Size: 304 213 4837
Fragments arranged by size:
4837 304 213
BseYI C'CCAG_C
Cuts at: 270 594 731 1416 1582 1747 2208 2779 3537
Size: 324 137 685 166 165 461 571 758
Cuts at: 3537 4202 4610 270
Size: 665 408 1014
Fragments arranged by size:
1014 758 685 665 571 461 408 324
166 165 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 556 1010 1015 1144 1375 1630 1792 1918
Size: 107 454 5 129 231 255 162 126
Cuts at: 1918 1937 449
Size: 19 3866
Fragments arranged by size:
3866 454 255 231 162 129 126 107
19 5
BsiEI CG_ry'CG
Cuts at: 104 205 2896 2942 4172 104
Size: 101 2691 46 1230 1286
Fragments arranged by size:
2691 1286 1230 101 46
BsiHKAI G_wGCw'C
Cuts at: 863 1439 1979 2368 3146 3336 4196 863
Size: 576 540 389 778 190 860 2021
Fragments arranged by size:
2021 860 778 576 540 389 190
Bsp1286I G_dGCh'C
Cuts at: 277 863 914 1439 1581 1667 1812 1979 2101
Size: 586 51 525 142 86 145 167 122
Cuts at: 2101 2255 2319 2368 2979 3072 3146 3336 3398
Size: 154 64 49 611 93 74 190 62
Cuts at: 3398 4196 277
Size: 798 1435
Fragments arranged by size:
1435 798 611 586 525 190 167 154
145 142 122 93 86 74 64 62
51 49
BspEI T'CCGG_A
Cuts at: 830 830
Size: 5354
BspHI T'CATG_A
Cuts at: 579 3786 579
Size: 3207 2147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 830 902 1049 1564 2193 2404 2618 2920 3301
Size: 72 147 515 629 211 214 302 381
Cuts at: 3301 830
Size: 2883
Fragments arranged by size:
2883 629 515 381 302 214 211 147
72
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2056 3266 2056
Size: 1210 4144
BsrFI r'CCGG_y
Cuts at: 381 1136 1896 3352 3533 381
Size: 755 760 1456 181 2202
Fragments arranged by size:
2202 1456 760 755 181
BsrGI T'GTAC_A
Cuts at: 2288 2288
Size: 5354
BssSI C'ACGA_G
Cuts at: 3625 4333 3625
Size: 708 4646
BstAPI GCAn_nnn'nTGC
Cuts at: 543 2731 543
Size: 2188 3166
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstXI CCAn_nnnn'nTGG
Cuts at: 2139 2139
Size: 5354
BstYI r'GATC_y
Cuts at: 1 1001 2379 2846 2869 3204 3450 3756 3768
Size: 1000 1378 467 23 335 246 306 12
Cuts at: 3768 3854 4906 1
Size: 86 1052 449
Fragments arranged by size:
1378 1052 1000 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1910 2153 1910
Size: 243 5111
BtgI C'CryG_G
Cuts at: 199 293 199
Size: 94 5260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 312 1816 3304 3485 3648 312
Size: 1504 1488 181 163 2018
Fragments arranged by size:
2018 1504 1488 181 163
BtsI GCAGTG_nn'
Cuts at: 321 950 1027 1118 1618 2029 2597 321
Size: 629 77 91 500 411 568 3078
Fragments arranged by size:
3078 629 568 500 411 91 77
DraI TTT'AAA
Cuts at: 1221 2353 1221
Size: 1132 4222
DraIII CAC_nnn'GTG
Cuts at: 1167 1506 1624 1167
Size: 339 118 4897
Fragments arranged by size:
4897 339 118
DrdI GACnn_nn'nnGTC
Cuts at: 3060 4404 3060
Size: 1344 4010
EaeI y'GGCC_r
Cuts at: 318 356 1118 1134 1615 2282 2334 2939 3113
Size: 38 762 16 481 667 52 605 174
Cuts at: 3113 3504 3531 4876 318
Size: 391 27 1345 796
Fragments arranged by size:
1345 796 762 667 605 481 391 174
52 38 27 16
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 656 1186 3377 3587 656
Size: 530 2191 210 2423
Fragments arranged by size:
2423 2191 530 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 3562 4304 4450 5164 262
Size: 3300 742 146 714 452
Fragments arranged by size:
3300 742 714 452 146
EcoICRI GAG'CTC
Cuts at: 2366 2366
Size: 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 589 631 646 1834 1975 2185 2341 3179
Size: 193 42 15 1188 141 210 156 838
Cuts at: 3179 3611 3964 4626 396
Size: 432 353 662 1124
Fragments arranged by size:
1188 1124 838 662 432 353 210 193
156 141 42 15
EcoO109I rG'GnC_Cy
Cuts at: 875 1410 1590 1906 2097 2098 2315 2316 2493
Size: 535 180 316 191 1 217 1 177
Cuts at: 2493 875
Size: 3736
Fragments arranged by size:
3736 535 316 217 191 180 177 1
1
EcoRI G'AATT_C
Cuts at: 1830 2358 1830
Size: 528 4826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1725 3373 1725
Size: 1648 3706
FseI GG_CCGG'CC
Cuts at: 385 1140 385
Size: 755 4599
FspI TGC'GCA
Cuts at: 754 3135 754
Size: 2381 2973
HaeII r_GCGC'y
Cuts at: 188 211 287 355 1086 2788 3036 4266 5037
Size: 23 76 68 731 1702 248 1230 771
Cuts at: 5037 188
Size: 505
Fragments arranged by size:
1702 1230 771 731 505 248 76 68
23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 1006 1038 3196 3226 3228 3258 796
Size: 32 178 32 2158 30 2 30 2892
Fragments arranged by size:
2892 2158 178 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1921 2145 2393 1921
Size: 224 248 4882
Fragments arranged by size:
4882 248 224
KasI G'GCGC_C
Cuts at: 2784 3032 2784
Size: 248 5106
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 4113 4297 248
Size: 3865 184 1305
Fragments arranged by size:
3865 1305 184
MscI TGG'CCA
Cuts at: 1120 1617 2284 2336 3115 1120
Size: 497 667 52 779 3359
Fragments arranged by size:
3359 779 667 497 52
MslI CAynn'nnrTG
Cuts at: 3343 3470 3343
Size: 127 5227
MspA1I CmG'CkG
Cuts at: 125 201 231 1525 1747 1756 2139 2163 2465
Size: 76 30 1294 222 9 383 24 302
Cuts at: 2465 2779 3139 3923 4168 125
Size: 314 360 784 245 1311
Fragments arranged by size:
1311 1294 784 383 360 314 302 245
222 76 30 24 9
NaeI GCC'GGC
Cuts at: 383 1138 1898 3535 383
Size: 755 760 1637 2202
Fragments arranged by size:
2202 1637 760 755
NarI GG'CG_CC
Cuts at: 2785 3033 2785
Size: 248 5106
NgoMIV G'CCGG_C
Cuts at: 381 1136 1896 3533 381
Size: 755 760 1637 2202
Fragments arranged by size:
2202 1637 760 755
NheI G'CTAG_C
Cuts at: 1465 1465
Size: 5354
NspI r_CATG'y
Cuts at: 1894 2407 2907 4510 1894
Size: 513 500 1603 2738
Fragments arranged by size:
2738 1603 513 500
PasI CC'CwG_GG
Cuts at: 1846 1846
Size: 5354
PciI A'CATG_T
Cuts at: 1890 4506 1890
Size: 2616 2738
PflMI CCAn_nnn'nTGG
Cuts at: 523 977 523
Size: 454 4900
PfoI T'CCnGG_A
Cuts at: 1070 1730 1070
Size: 660 4694
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PmlI CAC'GTG
Cuts at: 587 587
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 5322
PpuMI rG'GwC_Cy
Cuts at: 875 1410 1590 1906 875
Size: 535 180 316 4323
Fragments arranged by size:
4323 535 316 180
PspOMI G'GGCC_C
Cuts at: 2097 2315 2097
Size: 218 5136
PstI C_TGCA'G
Cuts at: 245 439 899 1746 2204 2346 2401 3086 245
Size: 194 460 847 458 142 55 685 2513
Fragments arranged by size:
2513 847 685 460 458 194 142 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 1747 1756 2139 2163 2779 3139 1747
Size: 9 383 24 616 360 3962
Fragments arranged by size:
3962 616 383 360 24 9
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 2368 2368
Size: 5354
SacII CC_GC'GG
Cuts at: 202 202
Size: 5354
SalI G'TCGA_C
Cuts at: 1919 2143 2391 1919
Size: 224 248 4882
Fragments arranged by size:
4882 248 224
SapI GCTCTTCn'nnn_
Cuts at: 3377 3587 3377
Size: 210 5144
SbfI CC_TGCA'GG
Cuts at: 2401 2401
Size: 5354
ScaI AGT'ACT
Cuts at: 4871 4871
Size: 5354
SfcI C'TryA_G
Cuts at: 33 241 435 895 1209 1742 2200 2342 2397
Size: 208 194 460 314 533 458 142 55
Cuts at: 2397 3082 4050 4241 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 533 460 458 314 208
194 191 142 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 2333 2333
Size: 5354
SfoI GGC'GCC
Cuts at: 2786 3034 2786
Size: 248 5106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 1180 1699 2031 2509 3424 4138 4400 1180
Size: 519 332 478 915 714 262 2134
Fragments arranged by size:
2134 915 714 519 478 332 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 2407 2907 2407
Size: 500 4854
StuI AGG'CCT
Cuts at: 484 484
Size: 5354
StyI C'CwwG_G
Cuts at: 1397 1403 1604 2488 2842 1397
Size: 6 201 884 354 3909
Fragments arranged by size:
3909 884 354 201 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2937 3070 3529 3738 2937
Size: 133 459 209 4553
Fragments arranged by size:
4553 459 209 133
TatI w'GTAC_w
Cuts at: 2288 4869 2288
Size: 2581 2773
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1233 1265 4871 4903 1233
Size: 32 3606 32 1684
Fragments arranged by size:
3606 1684 32 32
Tth111I GACn'n_nGTC
Cuts at: 3151 3151
Size: 5354
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1005 1503 2849 1005
Size: 498 1346 3510
Fragments arranged by size:
3510 1346 498
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
ZraI GAC'GTC
Cuts at: 366 366
Size: 5354
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AscI AvrII BaeI
BfrBI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI