Restriction map for
pF1KB4141
(Circular) (Six-base) MAPSORT of: pF1KB4141.seq Check: 7312 from: 1 to: 5360
pF1KB4141 5360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2014 2014
Size: 5360
AccI GT'mk_AC
Cuts at: 2398 2398
Size: 5360
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 1650 2050 2109 3185 3617 3970 4632 590
Size: 1060 400 59 1076 432 353 662 1318
Fragments arranged by size:
1318 1076 1060 662 432 400 353 59
AfeI AGC'GCT
Cuts at: 1096 1096
Size: 5360
AflIII A'CryG_T
Cuts at: 2712 4512 4793 2712
Size: 1800 281 3279
Fragments arranged by size:
3279 1800 281
AleI CACnn'nnGTG
Cuts at: 665 665
Size: 5360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1352 1828 1352
Size: 476 4884
AlwNI CAG_nnn'CTG
Cuts at: 172 880 1383 2665 4103 172
Size: 708 503 1282 1438 1429
Fragments arranged by size:
1438 1429 1282 708 503
ApaI G_GGCC'C
Cuts at: 547 1187 1578 547
Size: 640 391 4329
Fragments arranged by size:
4329 640 391
ApaLI G'TGCA_C
Cuts at: 482 4198 482
Size: 3716 1644
ApoI r'AATT_y
Cuts at: 1359 2364 4713 4969 1359
Size: 1005 2349 256 1750
Fragments arranged by size:
2349 1750 1005 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 193 235 700 856 970 1570 1932 2380 193
Size: 42 465 156 114 600 362 448 3173
Fragments arranged by size:
3173 600 465 448 362 156 114 42
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 724 1975 2376 2790 3038 3073 724
Size: 1251 401 414 248 35 3011
Fragments arranged by size:
3011 1251 414 401 248 35
BanII G_rGCy'C
Cuts at: 194 274 547 623 1187 1578 1876 2374 3404
Size: 80 273 76 564 391 298 498 1030
Cuts at: 3404 194
Size: 2150
Fragments arranged by size:
2150 1030 564 498 391 298 273 80
76
BbeI G_GCGC'C
Cuts at: 728 2794 3042 728
Size: 2066 248 3046
Fragments arranged by size:
3046 2066 248
BbsI GAAGACnn'nnnn_
Cuts at: 1205 1721 2302 1205
Size: 516 581 4263
Fragments arranged by size:
4263 581 516
BciVI GTATCCnnnnn_n'
Cuts at: 3258 4314 5360 3258
Size: 1056 1046 3258
Fragments arranged by size:
3258 1056 1046
BclI T'GATC_A
Cuts at: 2880 2880
Size: 5360
BglI GCCn_nnn'nGGC
Cuts at: 847 1044 847
Size: 197 5163
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 1942 2174 2472 1942
Size: 232 298 4830
Fragments arranged by size:
4830 298 232
Bme1580I G_kGCm'C
Cuts at: 236 486 547 1187 1578 2985 3078 4202 236
Size: 250 61 640 391 1407 93 1124 1394
Fragments arranged by size:
1407 1394 1124 640 391 250 93 61
BmrI ACTGGGnnnn_n'
Cuts at: 1641 2572 2653 2988 5132 1641
Size: 931 81 335 2144 1869
Fragments arranged by size:
2144 1869 931 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3727 3727
Size: 5360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 306 784 1093 1840 265
Size: 41 478 309 747 3785
Fragments arranged by size:
3785 747 478 309 41
Bpu10I CC'TnA_GC
Cuts at: 869 869
Size: 5360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 652 1740 2092 2536 3415 4129 4427 145
Size: 507 1088 352 444 879 714 298 1078
Fragments arranged by size:
1088 1078 879 714 507 444 352 298
BsaI GGTCTCn'nnnn_
Cuts at: 156 983 1555 2332 5064 156
Size: 827 572 777 2732 452
Fragments arranged by size:
2732 827 777 572 452
BsaAI yAC'GTr
Cuts at: 336 2713 336
Size: 2377 2983
BsaBI GATnn'nnATC
Cuts at: 6 2874 4911 6
Size: 2868 2037 455
Fragments arranged by size:
2868 2037 455
BsaHI Gr'CG_yC
Cuts at: 725 1524 2186 2791 3039 3741 5237 725
Size: 799 662 605 248 702 1496 848
Fragments arranged by size:
1496 848 799 702 662 605 248
BsaWI w'CCGG_w
Cuts at: 116 609 2549 3070 3748 4159 4306 4580 116
Size: 493 1940 521 678 411 147 274 896
Fragments arranged by size:
1940 896 678 521 493 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 370 397 400 403 478 538 718 790
Size: 3 27 3 3 75 60 180 72
Cuts at: 790 2314 367
Size: 1524 3413
Fragments arranged by size:
3413 1524 180 75 72 60 27 3
3 3
BseYI C'CCAG_C
Cuts at: 928 1247 1634 1868 2118 2785 3543 4208 4616
Size: 319 387 234 250 667 758 665 408
Cuts at: 4616 928
Size: 1672
Fragments arranged by size:
1672 758 667 665 408 387 319 250
234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 2152
Size: 5360
BsiEI CG_ry'CG
Cuts at: 104 2279 2902 2948 4178 104
Size: 2175 623 46 1230 1286
Fragments arranged by size:
2175 1286 1230 623 46
BsiHKAI G_wGCw'C
Cuts at: 486 2374 3152 3342 4202 486
Size: 1888 778 190 860 1644
Fragments arranged by size:
1888 1644 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 194 236 274 486 547 623 1187 1578 1876
Size: 42 38 212 61 76 564 391 298
Cuts at: 1876 2374 2985 3078 3152 3342 3404 4202 194
Size: 498 611 93 74 190 62 798 1352
Fragments arranged by size:
1352 798 611 564 498 391 298 212
190 93 76 74 62 61 42 38
BspEI T'CCGG_A
Cuts at: 609 609
Size: 5360
BspHI T'CATG_A
Cuts at: 2156 3792 2156
Size: 1636 3724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1047 2014 2410 2624 2926 3307 1047
Size: 967 396 214 302 381 3100
Fragments arranged by size:
3100 967 396 381 302 214
BsrBI CCG'CTC
Cuts at: 50 140 1931 3652 3706 5190 5355 50
Size: 90 1791 1721 54 1484 165 55
Fragments arranged by size:
1791 1721 1484 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 1071 2168 3272 1071
Size: 1097 1104 3159
Fragments arranged by size:
3159 1104 1097
BsrFI r'CCGG_y
Cuts at: 721 3358 3539 721
Size: 2637 181 2542
Fragments arranged by size:
2637 2542 181
BsrGI T'GTAC_A
Cuts at: 1763 2348 1763
Size: 585 4775
BssSI C'ACGA_G
Cuts at: 562 937 1051 2245 3631 4339 562
Size: 375 114 1194 1386 708 1583
Fragments arranged by size:
1583 1386 1194 708 375 114
BstAPI GCAn_nnn'nTGC
Cuts at: 452 2737 452
Size: 2285 3075
BstBI TT'CG_AA
Cuts at: 3721 3721
Size: 5360
BstEII G'GTnAC_C
Cuts at: 2076 2076
Size: 5360
BstXI CCAn_nnnn'nTGG
Cuts at: 1236 1986 1236
Size: 750 4610
BstYI r'GATC_y
Cuts at: 1 1838 2285 2385 2852 2875 3210 3456 3762
Size: 1837 447 100 467 23 335 246 306
Cuts at: 3762 3774 3860 4912 1
Size: 12 86 1052 449
Fragments arranged by size:
1837 1052 467 449 447 335 306 246
100 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 380 422 742 881 380
Size: 42 320 139 4859
Fragments arranged by size:
4859 320 139 42
BtgI C'CryG_G
Cuts at: 107 555 1886 2081 107
Size: 448 1331 195 3386
Fragments arranged by size:
3386 1331 448 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1470 1626 3310 3491 3654 1470
Size: 156 1684 181 163 3176
Fragments arranged by size:
3176 1684 181 163 156
BtsI GCAGTG_nn'
Cuts at: 2603 2603
Size: 5360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1130 1151 1165 1186 1130
Size: 21 14 21 5304
Fragments arranged by size:
5304 21 21 14
DraI TTT'AAA
Cuts at: 1430 2359 1430
Size: 929 4431
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 5360
DrdI GACnn_nn'nnGTC
Cuts at: 3066 4410 3066
Size: 1344 4016
EaeI y'GGCC_r
Cuts at: 262 558 594 1517 1656 1898 2098 2234 2945
Size: 296 36 923 139 242 200 136 711
Cuts at: 2945 3119 3510 3537 4882 262
Size: 174 391 27 1345 740
Fragments arranged by size:
1345 923 740 711 391 296 242 200
174 139 136 36 27
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 1025 1405 3383 3593 1025
Size: 380 1978 210 2792
Fragments arranged by size:
2792 1978 380 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3568 4310 4456 5170 3568
Size: 742 146 714 3758
Fragments arranged by size:
3758 742 714 146
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 5360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 306 590 784 1093 1650 1840 2050 2109
Size: 41 284 194 309 557 190 210 59
Cuts at: 2109 3185 3617 3970 4632 265
Size: 1076 432 353 662 993
Fragments arranged by size:
1076 993 662 557 432 353 309 284
210 194 190 59 41
EcoNI CCTnn'n_nnAGG
Cuts at: 379 872 970 379
Size: 493 98 4769
Fragments arranged by size:
4769 493 98
EcoO109I rG'GnC_Cy
Cuts at: 169 240 372 759 877 1022 1183 1184 1574
Size: 71 132 387 118 145 161 1 390
Cuts at: 1574 2025 2499 169
Size: 451 474 3030
Fragments arranged by size:
3030 474 451 390 387 161 145 132
118 71 1
EcoRI G'AATT_C
Cuts at: 1359 2364 1359
Size: 1005 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5360
FspI TGC'GCA
Cuts at: 3141 3141
Size: 5360
HaeII r_GCGC'y
Cuts at: 728 842 1098 2794 3042 4272 5043 728
Size: 114 256 1696 248 1230 771 1045
Fragments arranged by size:
1696 1230 1045 771 256 248 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 304 310 336 342 607 639 2171
Size: 32 119 6 26 6 265 32 1532
Cuts at: 2171 2203 3202 3232 3234 3264 153
Size: 32 999 30 2 30 2249
Fragments arranged by size:
2249 1532 999 265 119 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 1119 2399 1119
Size: 1280 4080
HindIII A'AGCT_T
Cuts at: 121 121
Size: 5360
KasI G'GCGC_C
Cuts at: 724 2790 3038 724
Size: 2066 248 3046
Fragments arranged by size:
3046 2066 248
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 128 1604 2259 2294 4119 4303 128
Size: 1476 655 35 1825 184 1185
Fragments arranged by size:
1825 1476 1185 655 184 35
MscI TGG'CCA
Cuts at: 1519 1900 2236 3121 1519
Size: 381 336 885 3758
Fragments arranged by size:
3758 885 381 336
MslI CAynn'nnrTG
Cuts at: 665 1285 1507 3349 3476 665
Size: 620 222 1842 127 2549
Fragments arranged by size:
2549 1842 620 222 127
MspA1I CmG'CkG
Cuts at: 920 1251 1818 2066 2118 2346 2471 2785 3145
Size: 331 567 248 52 228 125 314 360
Cuts at: 3145 3929 4174 920
Size: 784 245 2106
Fragments arranged by size:
2106 784 567 360 331 314 248 245
228 125 52
NaeI GCC'GGC
Cuts at: 3541 3541
Size: 5360
NarI GG'CG_CC
Cuts at: 725 2791 3039 725
Size: 2066 248 3046
Fragments arranged by size:
3046 2066 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5360
NgoMIV G'CCGG_C
Cuts at: 3539 3539
Size: 5360
NspI r_CATG'y
Cuts at: 147 1533 2413 2913 4516 147
Size: 1386 880 500 1603 991
Fragments arranged by size:
1603 1386 991 880 500
PciI A'CATG_T
Cuts at: 4512 4512
Size: 5360
PflMI CCAn_nnn'nTGG
Cuts at: 795 795
Size: 5360
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 331 363 3806 3838 331
Size: 32 3443 32 1853
Fragments arranged by size:
3443 1853 32 32
PpuMI rG'GwC_Cy
Cuts at: 169 759 1022 169
Size: 590 263 4507
Fragments arranged by size:
4507 590 263
PspOMI G'GGCC_C
Cuts at: 543 1183 1574 543
Size: 640 391 4329
Fragments arranged by size:
4329 640 391
PstI C_TGCA'G
Cuts at: 925 1817 2152 2407 3092 925
Size: 892 335 255 685 3193
Fragments arranged by size:
3193 892 685 335 255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 920 1251 1818 2066 2118 2785 3145 920
Size: 331 567 248 52 667 360 3135
Fragments arranged by size:
3135 667 567 360 331 248 52
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 5360
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 3383 3593 3383
Size: 210 5150
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SexAI A'CCwGG_T
Cuts at: 165 165
Size: 5360
SfcI C'TryA_G
Cuts at: 33 890 921 1813 2148 2403 3088 4056 4247
Size: 857 31 892 335 255 685 968 191
Cuts at: 4247 33
Size: 1146
Fragments arranged by size:
1146 968 892 857 685 335 255 191
31
SfiI GGCCn_nnn'nGGCC
Cuts at: 1044 1044
Size: 5360
SfoI GGC'GCC
Cuts at: 726 2792 3040 726
Size: 2066 248 3046
Fragments arranged by size:
3046 2066 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SgrAI Cr'CCGG_yG
Cuts at: 721 721
Size: 5360
SmaI CCC'GGG
Cuts at: 1572 2382 1572
Size: 810 4550
SmlI C'TyrA_G
Cuts at: 124 631 1719 2107 2515 3430 4144 4406 124
Size: 507 1088 388 408 915 714 262 1078
Fragments arranged by size:
1088 1078 915 714 507 408 388 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 1533 2413 2913 1533
Size: 880 500 3980
Fragments arranged by size:
3980 880 500
StuI AGG'CCT
Cuts at: 898 1663 898
Size: 765 4595
StyI C'CwwG_G
Cuts at: 107 218 1081 2010 2494 2848 107
Size: 111 863 929 484 354 2619
Fragments arranged by size:
2619 929 863 484 354 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 921 1261 1426 1768 2265 2943 3076 3535 3744
Size: 340 165 342 497 678 133 459 209
Cuts at: 3744 921
Size: 2537
Fragments arranged by size:
2537 678 497 459 342 340 209 165
133
TatI w'GTAC_w
Cuts at: 1763 2348 4875 1763
Size: 585 2527 2248
Fragments arranged by size:
2527 2248 585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 4877 4909 277
Size: 32 4568 32 728
Fragments arranged by size:
4568 728 32 32
Tth111I GACn'n_nGTC
Cuts at: 167 3157 167
Size: 2990 2370
XbaI T'CTAG_A
Cuts at: 2391 2391
Size: 5360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 310 652 1893 2855 310
Size: 342 1241 962 2815
Fragments arranged by size:
2815 1241 962 342
XmaI C'CCGG_G
Cuts at: 1570 2380 1570
Size: 810 4550
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BssHII BstZ17I ClaI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI