Restriction map for
pF1KB4144
(Circular) (Six-base) MAPSORT of: pF1KB4144.seq Check: 8151 from: 1 to: 3581
pF1KB4144 3581 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 236 236
Size: 3581
AccI GT'mk_AC
Cuts at: 619 619
Size: 3581
Acc65I G'GTAC_C
Cuts at: 597 597
Size: 3581
AclI AA'CG_TT
Cuts at: 3421 3421
Size: 3581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1406 1838 2585 2853 283
Size: 1123 432 747 268 1011
Fragments arranged by size:
1123 1011 747 432 268
AflIII A'CryG_T
Cuts at: 933 2037 3014 933
Size: 1104 977 1500
Fragments arranged by size:
1500 1104 977
AgeI A'CCGG_T
Cuts at: 1969 1969
Size: 3581
AleI CACnn'nnGTG
Cuts at: 553 553
Size: 3581
AlwNI CAG_nnn'CTG
Cuts at: 228 886 2453 228
Size: 658 1567 1356
Fragments arranged by size:
1567 1356 658
ApaLI G'TGCA_C
Cuts at: 2351 2351
Size: 3581
ApoI r'AATT_y
Cuts at: 585 2934 3190 585
Size: 2349 256 976
Fragments arranged by size:
2349 976 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
AvaI C'yCGr_G
Cuts at: 473 601 473
Size: 128 3453
BamHI G'GATC_C
Cuts at: 606 606
Size: 3581
BanI G'GyrC_C
Cuts at: 486 597 1011 1259 1294 486
Size: 111 414 248 35 2773
Fragments arranged by size:
2773 414 248 111 35
BanII G_rGCy'C
Cuts at: 542 595 1625 542
Size: 53 1030 2498
Fragments arranged by size:
2498 1030 53
BbeI G_GCGC'C
Cuts at: 490 1015 1263 490
Size: 525 248 2808
Fragments arranged by size:
2808 525 248
BciVI GTATCCnnnnn_n'
Cuts at: 1479 2240 3581 1479
Size: 761 1341 1479
Fragments arranged by size:
1479 1341 761
BclI T'GATC_A
Cuts at: 1101 1101
Size: 3581
BglII A'GATC_T
Cuts at: 1096 3133 1096
Size: 2037 1544
BlpI GC'TnA_GC
Cuts at: 178 693 178
Size: 515 3066
Bme1580I G_kGCm'C
Cuts at: 560 1206 1299 2355 560
Size: 646 93 1056 1786
Fragments arranged by size:
1786 1056 646 93
BmrI ACTGGGnnnn_n'
Cuts at: 793 874 1209 3353 793
Size: 81 335 2144 1021
Fragments arranged by size:
2144 1021 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 1948 561
Size: 1387 2194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 433 364
Size: 69 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 757 1636 2128 2426 268
Size: 489 879 492 298 1423
Fragments arranged by size:
1423 879 492 489 298
BsaI GGTCTCn'nnnn_
Cuts at: 241 3285 241
Size: 3044 537
BsaAI yAC'GTr
Cuts at: 934 934
Size: 3581
BsaBI GATnn'nnATC
Cuts at: 6 1095 3132 6
Size: 1089 2037 455
Fragments arranged by size:
2037 1089 455
BsaHI Gr'CG_yC
Cuts at: 422 487 1012 1260 1962 3458 422
Size: 65 525 248 702 1496 545
Fragments arranged by size:
1496 702 545 525 248 65
BsaWI w'CCGG_w
Cuts at: 770 1291 1969 2243 2390 2801 770
Size: 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 352 534 262
Size: 90 182 3309
Fragments arranged by size:
3309 182 90
BseYI C'CCAG_C
Cuts at: 1006 1764 2341 2837 1006
Size: 758 577 496 1750
Fragments arranged by size:
1750 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1123 1169 2377 104
Size: 1019 46 1208 1308
Fragments arranged by size:
1308 1208 1019 46
BsiHKAI G_wGCw'C
Cuts at: 179 542 595 1373 1563 2355 179
Size: 363 53 778 190 792 1405
Fragments arranged by size:
1405 792 778 363 190 53
Bsp1286I G_dGCh'C
Cuts at: 179 542 560 595 1206 1299 1373 1563 1625
Size: 363 18 35 611 93 74 190 62
Cuts at: 1625 2355 179
Size: 730 1405
Fragments arranged by size:
1405 730 611 363 190 93 74 62
35 18
BspEI T'CCGG_A
Cuts at: 2801 2801
Size: 3581
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 631 845 1147 1528 236
Size: 395 214 302 381 2289
Fragments arranged by size:
2289 395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 348 1873 1927 3411 3576 50
Size: 298 1525 54 1484 165 55
Fragments arranged by size:
1525 1484 298 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1493
Size: 3581
BsrFI r'CCGG_y
Cuts at: 1579 1760 1969 1579
Size: 181 209 3191
Fragments arranged by size:
3191 209 181
BssHII G'CGCG_C
Cuts at: 460 460
Size: 3581
BssSI C'ACGA_G
Cuts at: 1852 2210 1852
Size: 358 3223
BstAPI GCAn_nnn'nTGC
Cuts at: 958 958
Size: 3581
BstBI TT'CG_AA
Cuts at: 1942 1942
Size: 3581
BstEII G'GTnAC_C
Cuts at: 252 252
Size: 3581
BstYI r'GATC_y
Cuts at: 1 606 1073 1096 1431 1677 2689 2775 2787
Size: 605 467 23 335 246 1012 86 12
Cuts at: 2787 3133 1
Size: 346 449
Fragments arranged by size:
1012 605 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 291 467 291
Size: 176 3405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 197 1531 1712 1875 197
Size: 1334 181 163 1903
Fragments arranged by size:
1903 1334 181 163
BtsI GCAGTG_nn'
Cuts at: 824 824
Size: 3581
DraI TTT'AAA
Cuts at: 580 580
Size: 3581
DrdI GACnn_nn'nnGTC
Cuts at: 1287 2145 1287
Size: 858 2723
EaeI y'GGCC_r
Cuts at: 302 500 1166 1340 1731 1758 3103 302
Size: 198 666 174 391 27 1345 780
Fragments arranged by size:
1345 780 666 391 198 174 27
EagI C'GGCC_G
Cuts at: 1166 1166
Size: 3581
EarI CTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1789 2099 2245 3391 1789
Size: 310 146 1146 1979
Fragments arranged by size:
1979 1146 310 146
EcoICRI GAG'CTC
Cuts at: 540 593 540
Size: 53 3528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 364 433 1406 1838 2585 2853 283
Size: 81 69 973 432 747 268 1011
Fragments arranged by size:
1011 973 747 432 268 81 69
EcoO109I rG'GnC_Cy
Cuts at: 478 720 478
Size: 242 3339
EcoRI G'AATT_C
Cuts at: 585 585
Size: 3581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1600 1600
Size: 3581
FspI TGC'GCA
Cuts at: 1362 1362
Size: 3581
HaeII r_GCGC'y
Cuts at: 448 490 1015 1263 2285 3264 448
Size: 42 525 248 1022 979 765
Fragments arranged by size:
1022 979 765 525 248 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 181 213 1423 1453 1455 1485 102
Size: 32 47 32 1210 30 2 30 2198
Fragments arranged by size:
2198 1210 47 32 32 30 30 2
HincII GTy'rAC
Cuts at: 620 620
Size: 3581
KasI G'GCGC_C
Cuts at: 486 1011 1259 486
Size: 525 248 2808
Fragments arranged by size:
2808 525 248
KpnI G_GTAC'C
Cuts at: 601 601
Size: 3581
MluI A'CGCG_T
Cuts at: 3014 3014
Size: 3581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2436 2252
Size: 184 3397
MscI TGG'CCA
Cuts at: 502 1342 502
Size: 840 2741
MslI CAynn'nnrTG
Cuts at: 205 553 1570 1697 205
Size: 348 1017 127 2089
Fragments arranged by size:
2089 1017 348 127
MspA1I CmG'CkG
Cuts at: 293 308 692 1006 1366 2379 2624 293
Size: 15 384 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 384 360 314 245 15
NaeI GCC'GGC
Cuts at: 1762 1762
Size: 3581
NarI GG'CG_CC
Cuts at: 487 1012 1260 487
Size: 525 248 2808
Fragments arranged by size:
2808 525 248
NgoMIV G'CCGG_C
Cuts at: 1760 1760
Size: 3581
NspI r_CATG'y
Cuts at: 408 634 1134 2041 408
Size: 226 500 907 1948
Fragments arranged by size:
1948 907 500 226
PciI A'CATG_T
Cuts at: 2037 2037
Size: 3581
PmeI GTTT'AAAC
Cuts at: 580 580
Size: 3581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 3549
PpuMI rG'GwC_Cy
Cuts at: 478 478
Size: 3581
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 280 312 280
Size: 32 3549
PstI C_TGCA'G
Cuts at: 368 452 628 1313 368
Size: 84 176 685 2636
Fragments arranged by size:
2636 685 176 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3581
PvuII CAG'CTG
Cuts at: 308 1006 1366 308
Size: 698 360 2523
Fragments arranged by size:
2523 698 360
RsrII CG'GwC_CG
Cuts at: 1776 1776
Size: 3581
SacI G_AGCT'C
Cuts at: 542 595 542
Size: 53 3528
SacII CC_GC'GG
Cuts at: 294 294
Size: 3581
SalI G'TCGA_C
Cuts at: 618 618
Size: 3581
SapI GCTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
SbfI CC_TGCA'GG
Cuts at: 628 628
Size: 3581
ScaI AGT'ACT
Cuts at: 3098 3098
Size: 3581
SfcI C'TryA_G
Cuts at: 33 364 448 624 1309 2302 2493 33
Size: 331 84 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 331 191 176 84
SfoI GGC'GCC
Cuts at: 488 1013 1261 488
Size: 525 248 2808
Fragments arranged by size:
2808 525 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
SmaI CCC'GGG
Cuts at: 475 603 475
Size: 128 3453
SmlI C'TyrA_G
Cuts at: 283 736 1651 2143 2405 283
Size: 453 915 492 262 1459
Fragments arranged by size:
1459 915 492 453 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3581
SphI G_CATG'C
Cuts at: 408 634 1134 408
Size: 226 500 2855
Fragments arranged by size:
2855 500 226
StyI C'CwwG_G
Cuts at: 242 494 715 1069 242
Size: 252 221 354 2754
Fragments arranged by size:
2754 354 252 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1164 1297 1756 1965 1164
Size: 133 459 209 2780
Fragments arranged by size:
2780 459 209 133
TatI w'GTAC_w
Cuts at: 3096 3096
Size: 3581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3549
Tth111I GACn'n_nGTC
Cuts at: 1378 1378
Size: 3581
XbaI T'CTAG_A
Cuts at: 612 612
Size: 3581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1076 1076
Size: 3581
XmaI C'CCGG_G
Cuts at: 473 601 473
Size: 128 3453
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI