Restriction map for
pF1KB4146
(Circular) (Six-base) MAPSORT of: pF1KB4146.seq Check: 6329 from: 1 to: 4970
pF1KB4146 4970 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 2008 2008
Size: 4970
Acc65I G'GTAC_C
Cuts at: 712 1986 712
Size: 1274 3696
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 1070 2795 3227 3580 4242 517
Size: 553 1725 432 353 662 1245
Fragments arranged by size:
1725 1245 662 553 432 353
AflIII A'CryG_T
Cuts at: 2322 4122 4403 2322
Size: 1800 281 2889
Fragments arranged by size:
2889 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1202 1234 1202
Size: 32 4938
AlwNI CAG_nnn'CTG
Cuts at: 1345 2275 3713 1345
Size: 930 1438 2602
Fragments arranged by size:
2602 1438 930
ApaLI G'TGCA_C
Cuts at: 455 471 3808 455
Size: 16 3337 1617
Fragments arranged by size:
3337 1617 16
ApoI r'AATT_y
Cuts at: 394 1836 1974 4323 4579 394
Size: 1442 138 2349 256 785
Fragments arranged by size:
2349 1442 785 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 163 1990 163
Size: 1827 3143
BamHI G'GATC_C
Cuts at: 908 1995 908
Size: 1087 3883
BanI G'GyrC_C
Cuts at: 712 1986 2400 2648 2683 712
Size: 1274 414 248 35 2999
Fragments arranged by size:
2999 1274 414 248 35
BanII G_rGCy'C
Cuts at: 382 1254 1984 3014 382
Size: 872 730 1030 2338
Fragments arranged by size:
2338 1030 872 730
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BbsI GAAGACnn'nnnn_
Cuts at: 182 858 182
Size: 676 4294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 412 446 1577 1611 412
Size: 34 1131 34 3771
Fragments arranged by size:
3771 1131 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1667 2868 3924 4970 1667
Size: 1201 1056 1046 1667
Fragments arranged by size:
1667 1201 1056 1046
BclI T'GATC_A
Cuts at: 630 2490 630
Size: 1860 3110
BfrBI ATG'CAT
Cuts at: 1420 1574 1420
Size: 154 4816
BglII A'GATC_T
Cuts at: 687 2485 4522 687
Size: 1798 2037 1135
Fragments arranged by size:
2037 1798 1135
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 459 475 2595 2688 3812 459
Size: 16 2120 93 1124 1617
Fragments arranged by size:
2120 1617 1124 93 16
BmrI ACTGGGnnnn_n'
Cuts at: 1007 1867 2182 2263 2598 4742 1007
Size: 860 315 81 335 2144 1235
Fragments arranged by size:
2144 1235 860 335 315 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 322 3337 322
Size: 3015 1955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 317 820 1445 1703 1814 154
Size: 163 503 625 258 111 3310
Fragments arranged by size:
3310 625 503 258 163 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 2146 3025 3739 4037 1009
Size: 1137 879 714 298 1942
Fragments arranged by size:
1942 1137 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4674 4674
Size: 4970
BsaAI yAC'GTr
Cuts at: 2323 2323
Size: 4970
BsaBI GATnn'nnATC
Cuts at: 6 2484 4521 6
Size: 2478 2037 455
Fragments arranged by size:
2478 2037 455
BsaHI Gr'CG_yC
Cuts at: 2401 2649 3351 4847 2401
Size: 248 702 1496 2524
Fragments arranged by size:
2524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2159 2680 3358 3769 3916 4190 2159
Size: 521 678 411 147 274 2939
Fragments arranged by size:
2939 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1676 1706 1676
Size: 30 4940
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1444 1444
Size: 4970
BseYI C'CCAG_C
Cuts at: 830 2395 3153 3818 4226 830
Size: 1565 758 665 408 1574
Fragments arranged by size:
1574 1565 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 4970
BsiEI CG_ry'CG
Cuts at: 104 2512 2558 3788 104
Size: 2408 46 1230 1286
Fragments arranged by size:
2408 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 459 475 1865 1984 2762 2952 3812 459
Size: 16 1390 119 778 190 860 1617
Fragments arranged by size:
1617 1390 860 778 190 119 16
BsmI GAATG_Cn'
Cuts at: 455 1574 455
Size: 1119 3851
BsmBI CGTCTCn'nnnn_
Cuts at: 1027 1027
Size: 4970
Bsp1286I G_dGCh'C
Cuts at: 382 459 475 1254 1865 1984 2595 2688 2762
Size: 77 16 779 611 119 611 93 74
Cuts at: 2762 2952 3014 3812 382
Size: 190 62 798 1540
Fragments arranged by size:
1540 798 779 611 611 190 119 93
77 74 62 16
BspHI T'CATG_A
Cuts at: 1088 3402 1088
Size: 2314 2656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2020 2234 2536 2917 2020
Size: 214 302 381 4073
Fragments arranged by size:
4073 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3262 3316 4800 4965 50
Size: 3212 54 1484 165 55
Fragments arranged by size:
3212 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 813 1282 2882 813
Size: 469 1600 2901
Fragments arranged by size:
2901 1600 469
BsrFI r'CCGG_y
Cuts at: 2968 3149 2968
Size: 181 4789
BssSI C'ACGA_G
Cuts at: 3241 3949 3241
Size: 708 4262
BstAPI GCAn_nnn'nTGC
Cuts at: 2347 2347
Size: 4970
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 316 349 316
Size: 33 4937
BstYI r'GATC_y
Cuts at: 1 687 908 1842 1995 2462 2485 2820 3066
Size: 686 221 934 153 467 23 335 246
Cuts at: 3066 3372 3384 3470 4522 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 934 686 467 449 335 306 246
221 153 86 23 12
BtgI C'CryG_G
Cuts at: 579 903 579
Size: 324 4646
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 2213 2213
Size: 4970
DraI TTT'AAA
Cuts at: 1969 1969
Size: 4970
DraIII CAC_nnn'GTG
Cuts at: 800 1084 800
Size: 284 4686
DrdI GACnn_nn'nnGTC
Cuts at: 790 2676 4020 790
Size: 1886 1344 1740
Fragments arranged by size:
1886 1740 1344
EaeI y'GGCC_r
Cuts at: 1629 2555 2729 3120 3147 4492 1629
Size: 926 174 391 27 1345 2107
Fragments arranged by size:
2107 1345 926 391 174 27
EagI C'GGCC_G
Cuts at: 2555 2555
Size: 4970
EarI CTCTTCn'nnn_
Cuts at: 665 2993 3203 665
Size: 2328 210 2432
Fragments arranged by size:
2432 2328 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3178 3920 4066 4780 3178
Size: 742 146 714 3368
Fragments arranged by size:
3368 742 714 146
EcoICRI GAG'CTC
Cuts at: 1982 1982
Size: 4970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 317 517 820 1070 1445 1703 1814 2795
Size: 163 200 303 250 375 258 111 981
Cuts at: 2795 3227 3580 4242 154
Size: 432 353 662 882
Fragments arranged by size:
981 882 662 432 375 353 303 258
250 200 163 111
EcoO109I rG'GnC_Cy
Cuts at: 783 2109 783
Size: 1326 3644
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4970
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 2404 2652 3882 4653 2404
Size: 248 1230 771 2721
Fragments arranged by size:
2721 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 900 932 1636 1668 2812 2842 2844 2874 900
Size: 32 704 32 1144 30 2 30 2996
Fragments arranged by size:
2996 1144 704 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1449 1749 2009 1449
Size: 300 260 4410
Fragments arranged by size:
4410 300 260
HindIII A'AGCT_T
Cuts at: 752 752
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 716 1990 716
Size: 1274 3696
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 941 3729 3913 941
Size: 2788 184 1998
Fragments arranged by size:
2788 1998 184
MscI TGG'CCA
Cuts at: 1631 2731 1631
Size: 1100 3870
MslI CAynn'nnrTG
Cuts at: 1815 2959 3086 1815
Size: 1144 127 3699
Fragments arranged by size:
3699 1144 127
MspA1I CmG'CkG
Cuts at: 1013 1713 2081 2395 2755 3539 3784 1013
Size: 700 368 314 360 784 245 2199
Fragments arranged by size:
2199 784 700 368 360 314 245
NaeI GCC'GGC
Cuts at: 3151 3151
Size: 4970
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 579 903 579
Size: 324 4646
NgoMIV G'CCGG_C
Cuts at: 3149 3149
Size: 4970
NsiI A_TGCA'T
Cuts at: 1422 1576 1422
Size: 154 4816
NspI r_CATG'y
Cuts at: 1420 2023 2523 4126 1420
Size: 603 500 1603 2264
Fragments arranged by size:
2264 1603 603 500
PciI A'CATG_T
Cuts at: 4122 4122
Size: 4970
PflMI CCAn_nnn'nTGG
Cuts at: 1212 1212
Size: 4970
PfoI T'CCnGG_A
Cuts at: 1495 1495
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4938
PpuMI rG'GwC_Cy
Cuts at: 783 783
Size: 4970
PsiI TTA'TAA
Cuts at: 1517 1517
Size: 4970
PstI C_TGCA'G
Cuts at: 2017 2702 2017
Size: 685 4285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 1013 2395 2755 1013
Size: 1382 360 3228
Fragments arranged by size:
3228 1382 360
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 1984 1984
Size: 4970
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 2993 3203 2993
Size: 210 4760
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 4487 4487
Size: 4970
SfcI C'TryA_G
Cuts at: 33 400 1234 1407 2013 2698 3666 3857 33
Size: 367 834 173 606 685 968 191 1146
Fragments arranged by size:
1146 968 834 685 606 367 191 173
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 1024 2125 3040 3754 4016 1024
Size: 1101 915 714 262 1978
Fragments arranged by size:
1978 1101 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 1420 2023 2523 1420
Size: 603 500 3867
Fragments arranged by size:
3867 603 500
SspI AAT'ATT
Cuts at: 773 773
Size: 4970
StyI C'CwwG_G
Cuts at: 579 903 1607 2104 2458 579
Size: 324 704 497 354 3091
Fragments arranged by size:
3091 704 497 354 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1219 2553 2686 3145 3354 1219
Size: 1334 133 459 209 2835
Fragments arranged by size:
2835 1334 459 209 133
TatI w'GTAC_w
Cuts at: 4485 4485
Size: 4970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 560 592 4487 4519 560
Size: 32 3895 32 1011
Fragments arranged by size:
3895 1011 32 32
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 838 1599 1907 2465 838
Size: 761 308 558 3343
Fragments arranged by size:
3343 761 558 308
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
XmnI GAAnn'nnTTC
Cuts at: 530 1655 530
Size: 1125 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BaeI BbvCI BglI BmgBI BmtI
Bpu10I BsiWI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI