Restriction map for
pF1KB4148
(Circular) (Six-base) MAPSORT of: pF1KB4148.seq Check: 518 from: 1 to: 4685
pF1KB4148 4685 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 465 807 465
Size: 342 4343
AccI GT'mk_AC
Cuts at: 1076 1723 1076
Size: 647 4038
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 292 562 863 1292 2510 2942 3295 3957
Size: 44 270 301 429 1218 432 353 662
Cuts at: 3957 248
Size: 976
Fragments arranged by size:
1218 976 662 432 429 353 301 270
44
AflIII A'CryG_T
Cuts at: 2037 3837 4118 2037
Size: 1800 281 2604
Fragments arranged by size:
2604 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1464 1990 3428 1464
Size: 526 1438 2721
Fragments arranged by size:
2721 1438 526
ApaLI G'TGCA_C
Cuts at: 3523 3523
Size: 4685
ApoI r'AATT_y
Cuts at: 934 1331 1689 4038 4294 934
Size: 397 358 2349 256 1325
Fragments arranged by size:
2349 1325 397 358 256
AseI AT'TA_AT
Cuts at: 20 1039 20
Size: 1019 3666
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 128 169 1705 128
Size: 41 1536 3108
Fragments arranged by size:
3108 1536 41
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 1056 1701 2115 2363 2398 1056
Size: 645 414 248 35 3343
Fragments arranged by size:
3343 645 414 248 35
BanII G_rGCy'C
Cuts at: 975 1699 2729 975
Size: 724 1030 2931
Fragments arranged by size:
2931 1030 724
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbsI GAAGACnn'nnnn_
Cuts at: 1173 1286 1353 1173
Size: 113 67 4505
Fragments arranged by size:
4505 113 67
BciVI GTATCCnnnnn_n'
Cuts at: 245 2583 3639 4685 245
Size: 2338 1056 1046 245
Fragments arranged by size:
2338 1056 1046 245
BclI T'GATC_A
Cuts at: 2205 2205
Size: 4685
BglII A'GATC_T
Cuts at: 2200 4237 2200
Size: 2037 2648
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 2310 2403 3527 2310
Size: 93 1124 3468
Fragments arranged by size:
3468 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1897 1978 2313 4457 1897
Size: 81 335 2144 2125
Fragments arranged by size:
2144 2125 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 4685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1469 1469
Size: 4685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 1387 1861 2740 3454 3752 968
Size: 419 474 879 714 298 1901
Fragments arranged by size:
1901 879 714 474 419 298
BsaI GGTCTCn'nnnn_
Cuts at: 4389 4389
Size: 4685
BsaAI yAC'GTr
Cuts at: 1187 2038 1187
Size: 851 3834
BsaBI GATnn'nnATC
Cuts at: 6 2199 4236 6
Size: 2193 2037 455
Fragments arranged by size:
2193 2037 455
BsaHI Gr'CG_yC
Cuts at: 2116 2364 3066 4562 2116
Size: 248 702 1496 2239
Fragments arranged by size:
2239 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1874 2395 3073 3484 3631 3905 1874
Size: 521 678 411 147 274 2654
Fragments arranged by size:
2654 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1199 1229 1199
Size: 30 4655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1393 1393
Size: 4685
BseYI C'CCAG_C
Cuts at: 2110 2868 3533 3941 2110
Size: 758 665 408 2854
Fragments arranged by size:
2854 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 683 119
Size: 564 4121
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3503 104
Size: 2123 46 1230 1286
Fragments arranged by size:
2123 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 975 1699 2477 2667 3527 975
Size: 724 778 190 860 2133
Fragments arranged by size:
2133 860 778 724 190
BsmI GAATG_Cn'
Cuts at: 595 1586 595
Size: 991 3694
Bsp1286I G_dGCh'C
Cuts at: 975 1699 2310 2403 2477 2667 2729 3527 975
Size: 724 611 93 74 190 62 798 2133
Fragments arranged by size:
2133 798 724 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 975 3117 975
Size: 2142 2543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 465 807 1735 1949 2251 2632 465
Size: 342 928 214 302 381 2518
Fragments arranged by size:
2518 928 381 342 302 214
BsrBI CCG'CTC
Cuts at: 50 2977 3031 4515 4680 50
Size: 2927 54 1484 165 55
Fragments arranged by size:
2927 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2597 2597
Size: 4685
BsrFI r'CCGG_y
Cuts at: 123 2683 2864 123
Size: 2560 181 1944
Fragments arranged by size:
2560 1944 181
BsrGI T'GTAC_A
Cuts at: 1004 1602 1004
Size: 598 4087
BssSI C'ACGA_G
Cuts at: 2956 3664 2956
Size: 708 3977
BstAPI GCAn_nnn'nTGC
Cuts at: 1464 2062 1464
Size: 598 4087
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 1291 1291
Size: 4685
BstYI r'GATC_y
Cuts at: 1 1710 2177 2200 2535 2781 3087 3099 3185
Size: 1709 467 23 335 246 306 12 86
Cuts at: 3185 4237 1
Size: 1052 449
Fragments arranged by size:
1709 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 408 1653 107
Size: 301 1245 3139
Fragments arranged by size:
3139 1245 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 199 2635 2816 2979 199
Size: 2436 181 163 1905
Fragments arranged by size:
2436 1905 181 163
BtsI GCAGTG_nn'
Cuts at: 932 1928 932
Size: 996 3689
ClaI AT'CG_AT
Cuts at: 961 961
Size: 4685
DraI TTT'AAA
Cuts at: 707 1684 707
Size: 977 3708
DraIII CAC_nnn'GTG
Cuts at: 285 395 285
Size: 110 4575
DrdI GACnn_nn'nnGTC
Cuts at: 184 2391 3735 184
Size: 2207 1344 1134
Fragments arranged by size:
2207 1344 1134
EaeI y'GGCC_r
Cuts at: 110 411 2270 2444 2835 2862 4207 110
Size: 301 1859 174 391 27 1345 588
Fragments arranged by size:
1859 1345 588 391 301 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 1541 2708 2918 1541
Size: 1167 210 3308
Fragments arranged by size:
3308 1167 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3635 3781 4495 2893
Size: 742 146 714 3083
Fragments arranged by size:
3083 742 714 146
EcoICRI GAG'CTC
Cuts at: 973 1697 973
Size: 724 3961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 292 562 863 1292 1469 2510 2942 3295
Size: 44 270 301 429 177 1041 432 353
Cuts at: 3295 3957 248
Size: 662 976
Fragments arranged by size:
1041 976 662 432 429 353 301 270
177 44
EcoO109I rG'GnC_Cy
Cuts at: 1407 1824 1407
Size: 417 4268
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 2704 297
Size: 2407 2278
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 2119 2367 3597 4368 2119
Size: 248 1230 771 2436
Fragments arranged by size:
2436 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2527 2557 2559 2589 2527
Size: 30 2 30 4623
Fragments arranged by size:
4623 30 30 2
HincII GTy'rAC
Cuts at: 1724 1724
Size: 4685
HindIII A'AGCT_T
Cuts at: 662 674 662
Size: 12 4673
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MfeI C'AATT_G
Cuts at: 1340 1340
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1185 3444 3628 1185
Size: 2259 184 2242
Fragments arranged by size:
2259 2242 184
MscI TGG'CCA
Cuts at: 413 2446 413
Size: 2033 2652
MslI CAynn'nnrTG
Cuts at: 1423 2674 2801 1423
Size: 1251 127 3307
Fragments arranged by size:
3307 1251 127
MspA1I CmG'CkG
Cuts at: 1300 1796 2110 2470 3254 3499 1300
Size: 496 314 360 784 245 2486
Fragments arranged by size:
2486 784 496 360 314 245
NaeI GCC'GGC
Cuts at: 125 2866 125
Size: 2741 1944
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NcoI C'CATG_G
Cuts at: 107 408 107
Size: 301 4384
NgoMIV G'CCGG_C
Cuts at: 123 2864 123
Size: 2741 1944
NspI r_CATG'y
Cuts at: 1642 1738 2238 3841 1642
Size: 96 500 1603 2486
Fragments arranged by size:
2486 1603 500 96
PasI CC'CwG_GG
Cuts at: 257 257
Size: 4685
PciI A'CATG_T
Cuts at: 3837 3837
Size: 4685
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PmlI CAC'GTG
Cuts at: 1187 1187
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 4653
PsiI TTA'TAA
Cuts at: 914 914
Size: 4685
PstI C_TGCA'G
Cuts at: 1305 1540 1732 2417 1305
Size: 235 192 685 3573
Fragments arranged by size:
3573 685 235 192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 1300 2110 2470 1300
Size: 810 360 3515
Fragments arranged by size:
3515 810 360
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 975 1699 975
Size: 724 3961
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SfcI C'TryA_G
Cuts at: 33 996 1022 1301 1536 1728 2413 3381 3572
Size: 963 26 279 235 192 685 968 191
Cuts at: 3572 33
Size: 1146
Fragments arranged by size:
1146 968 963 685 279 235 192 191
26
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 130 171 1707 130
Size: 41 1536 3108
Fragments arranged by size:
3108 1536 41
SmlI C'TyrA_G
Cuts at: 983 1402 1840 2755 3469 3731 983
Size: 419 438 915 714 262 1937
Fragments arranged by size:
1937 915 714 438 419 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
SrfI GCCC'GGGC
Cuts at: 130 130
Size: 4685
SspI AAT'ATT
Cuts at: 610 1125 610
Size: 515 4170
StyI C'CwwG_G
Cuts at: 107 408 1819 2173 107
Size: 301 1411 354 2619
Fragments arranged by size:
2619 1411 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2268 2401 2860 3069 2268
Size: 133 459 209 3884
Fragments arranged by size:
3884 459 209 133
TatI w'GTAC_w
Cuts at: 1004 1602 4200 1004
Size: 598 2598 1489
Fragments arranged by size:
2598 1489 598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1199 1231 4202 4234 1199
Size: 32 2971 32 1650
Fragments arranged by size:
2971 1650 32 32
Tth111I GACn'n_nGTC
Cuts at: 181 2482 181
Size: 2301 2384
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1154 2180 1154
Size: 1026 3659
XmaI C'CCGG_G
Cuts at: 128 169 1705 128
Size: 41 1536 3108
Fragments arranged by size:
3108 1536 41
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BspEI BssHII BstEII
BstZ17I Bsu36I CspCI EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI StuI SwaI XhoI XmnI ZraI