Restriction map for
pF1KB4150
(Circular) (Six-base) MAPSORT of: pF1KB4150.seq Check: 1280 from: 1 to: 3494
pF1KB4150 3494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 466 1319 1751 2104 2766 404
Size: 62 853 432 353 662 1132
Fragments arranged by size:
1132 853 662 432 353 62
AflIII A'CryG_T
Cuts at: 293 846 2646 2927 293
Size: 553 1800 281 860
Fragments arranged by size:
1800 860 553 281
AlwNI CAG_nnn'CTG
Cuts at: 799 2237 799
Size: 1438 2056
ApaLI G'TGCA_C
Cuts at: 2332 2332
Size: 3494
ApoI r'AATT_y
Cuts at: 498 2847 3103 498
Size: 2349 256 889
Fragments arranged by size:
2349 889 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 514 514
Size: 3494
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 309 342 309
Size: 33 3461
BamHI G'GATC_C
Cuts at: 378 519 378
Size: 141 3353
BanI G'GyrC_C
Cuts at: 510 924 1172 1207 510
Size: 414 248 35 2797
Fragments arranged by size:
2797 414 248 35
BanII G_rGCy'C
Cuts at: 252 508 1538 252
Size: 256 1030 2208
Fragments arranged by size:
2208 1030 256
BbeI G_GCGC'C
Cuts at: 928 1176 928
Size: 248 3246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3460
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2448 3494 1392
Size: 1056 1046 1392
Fragments arranged by size:
1392 1056 1046
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglII A'GATC_T
Cuts at: 1009 3046 1009
Size: 2037 1457
BlpI GC'TnA_GC
Cuts at: 606 606
Size: 3494
Bme1580I G_kGCm'C
Cuts at: 1119 1212 2336 1119
Size: 93 1124 2277
Fragments arranged by size:
2277 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 1122 3266 706
Size: 81 335 2144 934
Fragments arranged by size:
2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 188
Size: 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1549 2263 2561 670
Size: 879 714 298 1603
Fragments arranged by size:
1603 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 847 847
Size: 3494
BsaBI GATnn'nnATC
Cuts at: 6 1008 3045 6
Size: 1002 2037 455
Fragments arranged by size:
2037 1002 455
BsaHI Gr'CG_yC
Cuts at: 925 1173 1875 3371 925
Size: 248 702 1496 1048
Fragments arranged by size:
1496 1048 702 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2293 2440 2714 683
Size: 521 678 411 147 274 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 919 1677 2342 2750 919
Size: 758 665 408 1663
Fragments arranged by size:
1663 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1036 1082 2312 104
Size: 932 46 1230 1286
Fragments arranged by size:
1286 1230 932 46
BsiHKAI G_wGCw'C
Cuts at: 508 1286 1476 2336 508
Size: 778 190 860 1666
Fragments arranged by size:
1666 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 252 508 1119 1212 1286 1476 1538 2336 252
Size: 256 611 93 74 190 62 798 1410
Fragments arranged by size:
1410 798 611 256 190 93 74 62
BspHI T'CATG_A
Cuts at: 1926 1926
Size: 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 758 1060 1441 544
Size: 214 302 381 2597
Fragments arranged by size:
2597 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1406 1406
Size: 3494
BsrFI r'CCGG_y
Cuts at: 1492 1673 1492
Size: 181 3313
BssSI C'ACGA_G
Cuts at: 1765 2473 1765
Size: 708 2786
BstAPI GCAn_nnn'nTGC
Cuts at: 871 871
Size: 3494
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstXI CCAn_nnnn'nTGG
Cuts at: 434 434
Size: 3494
BstYI r'GATC_y
Cuts at: 1 378 519 986 1009 1344 1590 1896 1908
Size: 377 141 467 23 335 246 306 12
Cuts at: 1908 1994 3046 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 449 377 335 306 246 141
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1444 1625 1788 1444
Size: 181 163 3150
Fragments arranged by size:
3150 181 163
BtsI GCAGTG_nn'
Cuts at: 156 737 156
Size: 581 2913
DraI TTT'AAA
Cuts at: 339 422 493 339
Size: 83 71 3340
Fragments arranged by size:
3340 83 71
DrdI GACnn_nn'nnGTC
Cuts at: 1200 2544 1200
Size: 1344 2150
EaeI y'GGCC_r
Cuts at: 1079 1253 1644 1671 3016 1079
Size: 174 391 27 1345 1557
Fragments arranged by size:
1557 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1079 1079
Size: 3494
EarI CTCTTCn'nnn_
Cuts at: 147 1517 1727 147
Size: 1370 210 1914
Fragments arranged by size:
1914 1370 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2444 2590 3304 1702
Size: 742 146 714 1892
Fragments arranged by size:
1892 742 714 146
EcoICRI GAG'CTC
Cuts at: 506 506
Size: 3494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 404 466 1319 1751 2104 2766 188
Size: 216 62 853 432 353 662 916
Fragments arranged by size:
916 853 662 432 353 216 62
EcoO109I rG'GnC_Cy
Cuts at: 633 633
Size: 3494
EcoRI G'AATT_C
Cuts at: 498 498
Size: 3494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1513
Size: 3494
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 928 1176 2406 3177 928
Size: 248 1230 771 1245
Fragments arranged by size:
1245 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1336 1366 1368 1398 1336
Size: 30 2 30 3432
Fragments arranged by size:
3432 30 30 2
HincII GTy'rAC
Cuts at: 171 533 171
Size: 362 3132
KasI G'GCGC_C
Cuts at: 924 1172 924
Size: 248 3246
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2253 2437 2253
Size: 184 3310
MscI TGG'CCA
Cuts at: 1255 1255
Size: 3494
MslI CAynn'nnrTG
Cuts at: 1483 1610 1483
Size: 127 3367
MspA1I CmG'CkG
Cuts at: 444 605 919 1279 2063 2308 444
Size: 161 314 360 784 245 1630
Fragments arranged by size:
1630 784 360 314 245 161
NaeI GCC'GGC
Cuts at: 1675 1675
Size: 3494
NarI GG'CG_CC
Cuts at: 925 1173 925
Size: 248 3246
NgoMIV G'CCGG_C
Cuts at: 1673 1673
Size: 3494
NruI TCG'CGA
Cuts at: 332 332
Size: 3494
NspI r_CATG'y
Cuts at: 297 547 1047 2650 297
Size: 250 500 1603 1141
Fragments arranged by size:
1603 1141 500 250
PciI A'CATG_T
Cuts at: 293 2646 293
Size: 2353 1141
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1940 1972 1940
Size: 32 3462
PstI C_TGCA'G
Cuts at: 541 1226 541
Size: 685 2809
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 444 919 1279 444
Size: 475 360 2659
Fragments arranged by size:
2659 475 360
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 508 508
Size: 3494
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 147 1517 1727 147
Size: 1370 210 1914
Fragments arranged by size:
1914 1370 210
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 3011 3011
Size: 3494
SfcI C'TryA_G
Cuts at: 33 537 1222 2190 2381 33
Size: 504 685 968 191 1146
Fragments arranged by size:
1146 968 685 504 191
SfoI GGC'GCC
Cuts at: 926 1174 926
Size: 248 3246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SmaI CCC'GGG
Cuts at: 516 516
Size: 3494
SmlI C'TyrA_G
Cuts at: 649 1564 2278 2540 649
Size: 915 714 262 1603
Fragments arranged by size:
1603 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
SspI AAT'ATT
Cuts at: 370 370
Size: 3494
StyI C'CwwG_G
Cuts at: 628 982 628
Size: 354 3140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1210 1669 1878 1077
Size: 133 459 209 2693
Fragments arranged by size:
2693 459 209 133
TatI w'GTAC_w
Cuts at: 3009 3009
Size: 3494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 989 989
Size: 3494
XmaI C'CCGG_G
Cuts at: 514 514
Size: 3494
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI