Restriction map for
pF1KB4151
(Circular) (Six-base) MAPSORT of: pF1KB4151.seq Check: 2197 from: 1 to: 6287
pF1KB4151 6287 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1920 1920
Size: 6287
AccI GT'mk_AC
Cuts at: 1874 3325 1874
Size: 1451 4836
Acc65I G'GTAC_C
Cuts at: 3303 3303
Size: 6287
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2029 4112 4544 4897 5559 2029
Size: 2083 432 353 662 2757
Fragments arranged by size:
2757 2083 662 432 353
AflIII A'CryG_T
Cuts at: 3639 5439 5720 3639
Size: 1800 281 4206
Fragments arranged by size:
4206 1800 281
AleI CACnn'nnGTG
Cuts at: 650 650
Size: 6287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2209 2209
Size: 6287
AlwNI CAG_nnn'CTG
Cuts at: 1045 1113 2283 2547 3592 5030 1045
Size: 68 1170 264 1045 1438 2302
Fragments arranged by size:
2302 1438 1170 1045 264 68
ApaI G_GGCC'C
Cuts at: 2342 2342
Size: 6287
ApaLI G'TGCA_C
Cuts at: 5125 5125
Size: 6287
ApoI r'AATT_y
Cuts at: 2500 3291 5640 5896 2500
Size: 791 2349 256 2891
Fragments arranged by size:
2891 2349 791 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 1208 2051 3307 1208
Size: 843 1256 4188
Fragments arranged by size:
4188 1256 843
BamHI G'GATC_C
Cuts at: 3312 3312
Size: 6287
BanI G'GyrC_C
Cuts at: 213 1290 1912 2852 3303 3717 3965 4000 213
Size: 1077 622 940 451 414 248 35 2500
Fragments arranged by size:
2500 1077 940 622 451 414 248 35
BanII G_rGCy'C
Cuts at: 521 1215 2052 2342 2594 3301 4331 521
Size: 694 837 290 252 707 1030 2477
Fragments arranged by size:
2477 1030 837 707 694 290 252
BbeI G_GCGC'C
Cuts at: 3721 3969 3721
Size: 248 6039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2032 2066 2032
Size: 34 6253
BciVI GTATCCnnnnn_n'
Cuts at: 4185 5241 6287 4185
Size: 1056 1046 4185
Fragments arranged by size:
4185 1056 1046
BclI T'GATC_A
Cuts at: 3807 3807
Size: 6287
BglI GCCn_nnn'nGGC
Cuts at: 350 433 1036 1149 350
Size: 83 603 113 5488
Fragments arranged by size:
5488 603 113 83
BglII A'GATC_T
Cuts at: 3802 5839 3802
Size: 2037 4250
BlpI GC'TnA_GC
Cuts at: 515 1130 3399 515
Size: 615 2269 3403
Fragments arranged by size:
3403 2269 615
Bme1580I G_kGCm'C
Cuts at: 209 424 1915 2342 2855 3912 4005 5129 209
Size: 215 1491 427 513 1057 93 1124 1367
Fragments arranged by size:
1491 1367 1124 1057 513 427 215 93
BmgBI CAC'GTC
Cuts at: 2938 2938
Size: 6287
BmrI ACTGGGnnnn_n'
Cuts at: 180 423 444 1918 2250 3499 3580 3915 6059
Size: 243 21 1474 332 1249 81 335 2144
Cuts at: 6059 180
Size: 408
Fragments arranged by size:
2144 1474 1249 408 335 332 243 81
21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4654 4654
Size: 6287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 634 1426 1915 2191 2848 301
Size: 333 792 489 276 657 3740
Fragments arranged by size:
3740 792 657 489 333 276
Bpu10I CC'TnA_GC
Cuts at: 1188 2316 1188
Size: 1128 5159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 1543 3112 3235 3463 4342 5056 5354 1414
Size: 129 1569 123 228 879 714 298 2347
Fragments arranged by size:
2347 1569 879 714 298 228 129 123
BsaI GGTCTCn'nnnn_
Cuts at: 5991 5991
Size: 6287
BsaAI yAC'GTr
Cuts at: 3198 3640 3198
Size: 442 5845
BsaBI GATnn'nnATC
Cuts at: 6 3801 5838 6
Size: 3795 2037 455
Fragments arranged by size:
3795 2037 455
BsaHI Gr'CG_yC
Cuts at: 2519 3718 3966 4668 6164 2519
Size: 1199 248 702 1496 2642
Fragments arranged by size:
2642 1496 1199 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1880 3476 3997 4675 5086 5233 5507 1256
Size: 624 1596 521 678 411 147 274 2036
Fragments arranged by size:
2036 1596 678 624 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 965 995 835
Size: 30 100 30 6127
Fragments arranged by size:
6127 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 1177 2602 3025 481
Size: 696 1425 423 3743
Fragments arranged by size:
3743 1425 696 423
BseYI C'CCAG_C
Cuts at: 1088 1279 1503 1581 2608 2781 3186 3712 4470
Size: 191 224 78 1027 173 405 526 758
Cuts at: 4470 5135 5543 1088
Size: 665 408 1832
Fragments arranged by size:
1832 1027 758 665 526 408 405 224
191 173 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1099 1246 1948 2200 2269 2509 2866 1078
Size: 21 147 702 252 69 240 357 4499
Fragments arranged by size:
4499 702 357 252 240 147 69 21
BsiEI CG_ry'CG
Cuts at: 104 3829 3875 5105 104
Size: 3725 46 1230 1286
Fragments arranged by size:
3725 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 521 599 1131 2036 2594 3056 3301 4079 4269
Size: 78 532 905 558 462 245 778 190
Cuts at: 4269 5129 521
Size: 860 1679
Fragments arranged by size:
1679 905 860 778 558 532 462 245
190 78
BsmI GAATG_Cn'
Cuts at: 1209 1209
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 209 424 521 599 1131 1215 1915 2036 2052
Size: 215 97 78 532 84 700 121 16
Cuts at: 2052 2342 2594 2855 3056 3301 3912 4005 4079
Size: 290 252 261 201 245 611 93 74
Cuts at: 4079 4269 4331 5129 209
Size: 190 62 798 1367
Fragments arranged by size:
1367 798 700 611 532 290 261 252
245 215 201 190 121 97 93 84
78 74 62 16
BspEI T'CCGG_A
Cuts at: 1256 1256
Size: 6287
BspHI T'CATG_A
Cuts at: 1334 4719 1334
Size: 3385 2902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 467 814 1499 1920 2481 3337 3551 3853 4234
Size: 347 685 421 561 856 214 302 381
Cuts at: 4234 467
Size: 2520
Fragments arranged by size:
2520 856 685 561 421 381 347 302
214
BsrBI CCG'CTC
Cuts at: 50 839 2361 3239 4579 4633 6117 6282 50
Size: 789 1522 878 1340 54 1484 165 55
Fragments arranged by size:
1522 1484 1340 878 789 165 55 54
BsrDI GCAATG_nn'
Cuts at: 311 1164 1929 2786 4199 311
Size: 853 765 857 1413 2399
Fragments arranged by size:
2399 1413 857 853 765
BsrFI r'CCGG_y
Cuts at: 1139 2744 4285 4466 1139
Size: 1605 1541 181 2960
Fragments arranged by size:
2960 1605 1541 181
BsrGI T'GTAC_A
Cuts at: 1853 2834 1853
Size: 981 5306
BssSI C'ACGA_G
Cuts at: 4558 5266 4558
Size: 708 5579
BstAPI GCAn_nnn'nTGC
Cuts at: 2040 2283 2487 3664 2040
Size: 243 204 1177 4663
Fragments arranged by size:
4663 1177 243 204
BstBI TT'CG_AA
Cuts at: 4648 4648
Size: 6287
BstEII G'GTnAC_C
Cuts at: 777 801 777
Size: 24 6263
BstXI CCAn_nnnn'nTGG
Cuts at: 228 333 1830 2124 2213 228
Size: 105 1497 294 89 4302
Fragments arranged by size:
4302 1497 294 105 89
BstYI r'GATC_y
Cuts at: 1 1284 2525 3312 3779 3802 4137 4383 4689
Size: 1283 1241 787 467 23 335 246 306
Cuts at: 4689 4701 4787 5839 1
Size: 12 86 1052 449
Fragments arranged by size:
1283 1241 1052 787 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1406 1406
Size: 6287
BtgI C'CryG_G
Cuts at: 651 899 969 2807 651
Size: 248 70 1838 4131
Fragments arranged by size:
4131 1838 248 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4237 4418 4581 4237
Size: 181 163 5943
Fragments arranged by size:
5943 181 163
BtsI GCAGTG_nn'
Cuts at: 249 422 539 1229 1378 1888 2050 2134 3530
Size: 173 117 690 149 510 162 84 1396
Cuts at: 3530 249
Size: 3006
Fragments arranged by size:
3006 1396 690 510 173 162 149 117
84
DraI TTT'AAA
Cuts at: 1017 3286 1017
Size: 2269 4018
DraIII CAC_nnn'GTG
Cuts at: 760 760
Size: 6287
DrdI GACnn_nn'nnGTC
Cuts at: 1417 3993 5337 1417
Size: 2576 1344 2367
Fragments arranged by size:
2576 2367 1344
EaeI y'GGCC_r
Cuts at: 230 1137 1141 1820 2468 3872 4046 4437 4464
Size: 907 4 679 648 1404 174 391 27
Cuts at: 4464 5809 230
Size: 1345 708
Fragments arranged by size:
1404 1345 907 708 679 648 391 174
27 4
EagI C'GGCC_G
Cuts at: 3872 3872
Size: 6287
EarI CTCTTCn'nnn_
Cuts at: 706 2077 4310 4520 706
Size: 1371 2233 210 2473
Fragments arranged by size:
2473 2233 1371 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4495 5237 5383 6097 4495
Size: 742 146 714 4685
Fragments arranged by size:
4685 742 714 146
EcoICRI GAG'CTC
Cuts at: 519 2592 3299 519
Size: 2073 707 3507
Fragments arranged by size:
3507 2073 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 634 1426 1915 2029 2191 2848 4112 4544
Size: 333 792 489 114 162 657 1264 432
Cuts at: 4544 4897 5559 301
Size: 353 662 1029
Fragments arranged by size:
1264 1029 792 662 657 489 432 353
333 162 114
EcoNI CCTnn'n_nnAGG
Cuts at: 463 562 814 1243 1426 463
Size: 99 252 429 183 5324
Fragments arranged by size:
5324 429 252 183 99
EcoO109I rG'GnC_Cy
Cuts at: 264 351 684 919 1110 1248 1314 1419 1765
Size: 87 333 235 191 138 66 105 346
Cuts at: 1765 1974 2493 3426 264
Size: 209 519 933 3125
Fragments arranged by size:
3125 933 519 346 333 235 209 191
138 105 87 66
EcoRI G'AATT_C
Cuts at: 2500 3291 2500
Size: 791 5496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1029 4306 1029
Size: 3277 3010
FseI GG_CCGG'CC
Cuts at: 1143 1143
Size: 6287
FspI TGC'GCA
Cuts at: 4068 4068
Size: 6287
HaeII r_GCGC'y
Cuts at: 1449 2326 2558 3208 3721 3969 5199 5970 1449
Size: 877 232 650 513 248 1230 771 1766
Fragments arranged by size:
1766 1230 877 771 650 513 248 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 835 867 965 997 1592 1624 2433
Size: 32 373 32 98 32 595 32 809
Cuts at: 2433 2465 3260 3292 4129 4159 4161 4191 430
Size: 32 795 32 837 30 2 30 2526
Fragments arranged by size:
2526 837 809 795 595 373 98 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2097 2376 3326 2097
Size: 279 950 5058
Fragments arranged by size:
5058 950 279
KasI G'GCGC_C
Cuts at: 3717 3965 3717
Size: 248 6039
KpnI G_GTAC'C
Cuts at: 3307 3307
Size: 6287
MluI A'CGCG_T
Cuts at: 5720 5720
Size: 6287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 974 983 2729 3119 5046 5230 974
Size: 9 1746 390 1927 184 2031
Fragments arranged by size:
2031 1927 1746 390 184 9
MscI TGG'CCA
Cuts at: 232 1822 2470 4048 232
Size: 1590 648 1578 2471
Fragments arranged by size:
2471 1590 1578 648
MslI CAynn'nnrTG
Cuts at: 650 1855 4276 4403 650
Size: 1205 2421 127 2534
Fragments arranged by size:
2534 2421 1205 127
MspA1I CmG'CkG
Cuts at: 123 282 624 1092 1711 1868 2280 3118 3398
Size: 159 342 468 619 157 412 838 280
Cuts at: 3398 3712 4072 4856 5101 123
Size: 314 360 784 245 1309
Fragments arranged by size:
1309 838 784 619 468 412 360 342
314 280 245 159 157
NaeI GCC'GGC
Cuts at: 1141 4468 1141
Size: 3327 2960
NarI GG'CG_CC
Cuts at: 3718 3966 3718
Size: 248 6039
NcoI C'CATG_G
Cuts at: 899 899
Size: 6287
NdeI CA'TA_TG
Cuts at: 1269 2681 1269
Size: 1412 4875
NgoMIV G'CCGG_C
Cuts at: 1139 4466 1139
Size: 3327 2960
NspI r_CATG'y
Cuts at: 1854 3340 3840 5443 1854
Size: 1486 500 1603 2698
Fragments arranged by size:
2698 1603 1486 500
PasI CC'CwG_GG
Cuts at: 1670 3081 1670
Size: 1411 4876
PciI A'CATG_T
Cuts at: 5439 5439
Size: 6287
PflMI CCAn_nnn'nTGG
Cuts at: 591 591
Size: 6287
PfoI T'CCnGG_A
Cuts at: 1348 1348
Size: 6287
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PmlI CAC'GTG
Cuts at: 3198 3198
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 4733
Size: 32 6255
PpuMI rG'GwC_Cy
Cuts at: 919 1248 1419 1765 1974 2493 919
Size: 329 171 346 209 519 4713
Fragments arranged by size:
4713 519 346 329 209 171
PshAI GACnn'nnGTC
Cuts at: 2579 2579
Size: 6287
PspOMI G'GGCC_C
Cuts at: 2338 2338
Size: 6287
PspXI vC'TCGA_Gb
Cuts at: 1208 1208
Size: 6287
PstI C_TGCA'G
Cuts at: 1710 2045 2201 2483 2552 2742 2999 3334 4019
Size: 335 156 282 69 190 257 335 685
Cuts at: 4019 1710
Size: 3978
Fragments arranged by size:
3978 685 335 335 282 257 190 156
69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6287
PvuII CAG'CTG
Cuts at: 282 624 1092 1711 2280 3118 3712 4072 282
Size: 342 468 619 569 838 594 360 2497
Fragments arranged by size:
2497 838 619 594 569 468 360 342
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 521 2594 3301 521
Size: 2073 707 3507
Fragments arranged by size:
3507 2073 707
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 706 4310 4520 706
Size: 3604 210 2473
Fragments arranged by size:
3604 2473 210
SbfI CC_TGCA'GG
Cuts at: 3334 3334
Size: 6287
ScaI AGT'ACT
Cuts at: 2260 2947 5804 2260
Size: 687 2857 2743
Fragments arranged by size:
2857 2743 687
SfcI C'TryA_G
Cuts at: 33 1706 2041 2197 2479 2548 2738 2995 3330
Size: 1673 335 156 282 69 190 257 335
Cuts at: 3330 4015 4983 5174 33
Size: 685 968 191 1146
Fragments arranged by size:
1673 1146 968 685 335 335 282 257
191 190 156 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 350 1149 350
Size: 799 5488
SfoI GGC'GCC
Cuts at: 3719 3967 3719
Size: 248 6039
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SmaI CCC'GGG
Cuts at: 3309 3309
Size: 6287
SmlI C'TyrA_G
Cuts at: 1208 1393 1558 3127 3214 3442 4357 5071 5333
Size: 185 165 1569 87 228 915 714 262
Cuts at: 5333 1208
Size: 2162
Fragments arranged by size:
2162 1569 915 714 262 228 185 165
87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 3340 3840 3340
Size: 500 5787
StuI AGG'CCT
Cuts at: 344 1342 2069 344
Size: 998 727 4562
Fragments arranged by size:
4562 998 727
StyI C'CwwG_G
Cuts at: 384 782 899 1022 2159 3421 3775 384
Size: 398 117 123 1137 1262 354 2896
Fragments arranged by size:
2896 1262 1137 398 354 123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 622 1308 1519 2269 2471 3870 4003 4462 4671
Size: 686 211 750 202 1399 133 459 209
Cuts at: 4671 622
Size: 2238
Fragments arranged by size:
2238 1399 750 686 459 211 209 202
133
TatI w'GTAC_w
Cuts at: 864 1853 2258 2834 2945 5802 864
Size: 989 405 576 111 2857 1349
Fragments arranged by size:
2857 1349 989 576 405 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5804 5836 5804
Size: 32 6255
Tth111I GACn'n_nGTC
Cuts at: 847 1953 4084 847
Size: 1106 2131 3050
Fragments arranged by size:
3050 2131 1106
XbaI T'CTAG_A
Cuts at: 2583 3318 2583
Size: 735 5552
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 609 1096 1578 1810 2805 3782 609
Size: 487 482 232 995 977 3114
Fragments arranged by size:
3114 995 977 487 482 232
XhoI C'TCGA_G
Cuts at: 1208 1208
Size: 6287
XmaI C'CCGG_G
Cuts at: 3307 3307
Size: 6287
XmnI GAAnn'nnTTC
Cuts at: 1519 2014 2881 1519
Size: 495 867 4925
Fragments arranged by size:
4925 867 495
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BsiWI BsmBI BssHII
BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PsiI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI ZraI