Restriction map for
pF1KB4155
(Circular) (Six-base) MAPSORT of: pF1KB4155.seq Check: 6245 from: 1 to: 4010
pF1KB4155 4010 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 639 1835 2267 3014 3282 236
Size: 403 1196 432 747 268 964
Fragments arranged by size:
1196 964 747 432 403 268
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 721 1315 2882 721
Size: 594 1567 1849
Fragments arranged by size:
1849 1567 594
ApaLI G'TGCA_C
Cuts at: 199 2780 199
Size: 2581 1429
ApoI r'AATT_y
Cuts at: 224 976 1014 3363 3619 224
Size: 752 38 2349 256 615
Fragments arranged by size:
2349 752 615 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 264 1030 264
Size: 766 3244
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 1024 2054 1024
Size: 1030 2980
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbsI GAAGACnn'nnnn_
Cuts at: 419 419
Size: 4010
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 203 963 1635 1728 2784 203
Size: 760 672 93 1056 1429
Fragments arranged by size:
1429 1056 760 672 93
BmrI ACTGGGnnnn_n'
Cuts at: 428 1222 1303 1638 3782 428
Size: 794 81 335 2144 656
Fragments arranged by size:
2144 794 656 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 749
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 444 3714 444
Size: 3270 740
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 280 310 511 541 683 713 280
Size: 30 201 30 142 30 3577
Fragments arranged by size:
3577 201 142 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 519 519
Size: 4010
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 175 203 1024 1802 1992 2784 175
Size: 28 821 778 190 792 1401
Fragments arranged by size:
1401 821 792 778 190 28
Bsp1286I G_dGCh'C
Cuts at: 175 203 963 1024 1635 1728 1802 1992 2054
Size: 28 760 61 611 93 74 190 62
Cuts at: 2054 2784 175
Size: 730 1401
Fragments arranged by size:
1401 760 730 611 190 93 74 62
61 28
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 807 2302 2356 3840 4005 50
Size: 757 1495 54 1484 165 55
Fragments arranged by size:
1495 1484 757 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstEII G'GTnAC_C
Cuts at: 366 366
Size: 4010
BstYI r'GATC_y
Cuts at: 1 1035 1502 1525 1860 2106 3118 3204 3216
Size: 1034 467 23 335 246 1012 86 12
Cuts at: 3216 3562 1
Size: 346 449
Fragments arranged by size:
1034 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 195 560 1253 195
Size: 365 693 2952
Fragments arranged by size:
2952 693 365
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 1595 1769 2160 2187 3532 1595
Size: 174 391 27 1345 2073
Fragments arranged by size:
2073 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 154 210 669 2033 2243 154
Size: 56 459 1364 210 1921
Fragments arranged by size:
1921 1364 459 210 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 639 749 1835 2267 3014 3282 236
Size: 403 110 1086 432 747 268 964
Fragments arranged by size:
1086 964 747 432 403 268 110
EcoO109I rG'GnC_Cy
Cuts at: 1149 1149
Size: 4010
EcoRI G'AATT_C
Cuts at: 224 976 1014 224
Size: 752 38 3220
Fragments arranged by size:
3220 752 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 790 1791 790
Size: 1001 3009
FspAI rTGC'GCAy
Cuts at: 790 790
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 683 697 715 729 1852 1882 1884 1914 683
Size: 14 18 14 1123 30 2 30 2779
Fragments arranged by size:
2779 1123 30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 519 1049 519
Size: 530 3480
HpaI GTT'AAC
Cuts at: 519 519
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 433 1999 2126 433
Size: 1566 127 2317
Fragments arranged by size:
2317 1566 127
MspA1I CmG'CkG
Cuts at: 740 1121 1435 1795 2808 3053 740
Size: 381 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 381 360 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 107
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 566 1063 1563 2470 566
Size: 497 500 907 2106
Fragments arranged by size:
2106 907 500 497
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PstI C_TGCA'G
Cuts at: 1057 1742 1057
Size: 685 3325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 740 1435 1795 740
Size: 695 360 2955
Fragments arranged by size:
2955 695 360
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SexAI A'CCwGG_T
Cuts at: 601 601
Size: 4010
SfcI C'TryA_G
Cuts at: 33 512 888 1053 1738 2731 2922 33
Size: 479 376 165 685 993 191 1121
Fragments arranged by size:
1121 993 685 479 376 191 165
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 107 303 1144 1498 107
Size: 196 841 354 2619
Fragments arranged by size:
2619 841 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 783 1593 1726 2185 2394 783
Size: 810 133 459 209 2399
Fragments arranged by size:
2399 810 459 209 133
TatI w'GTAC_w
Cuts at: 3525 3525
Size: 4010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 990 1807 990
Size: 817 3193
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI