Restriction map for
pF1KB4158
(Circular) (Six-base) MAPSORT of: pF1KB4158.seq Check: 1675 from: 1 to: 4589
pF1KB4158 4589 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 456 971 1627 456
Size: 515 656 3418
Fragments arranged by size:
3418 656 515
Acc65I G'GTAC_C
Cuts at: 1605 1605
Size: 4589
AclI AA'CG_TT
Cuts at: 930 4429 930
Size: 3499 1090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 857 928 1316 2414 2846 3199 3861 538
Size: 319 71 388 1098 432 353 662 1266
Fragments arranged by size:
1266 1098 662 432 388 353 319 71
AflIII A'CryG_T
Cuts at: 1941 3741 4022 1941
Size: 1800 281 2508
Fragments arranged by size:
2508 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1492 1492
Size: 4589
AlwNI CAG_nnn'CTG
Cuts at: 1894 3332 1894
Size: 1438 3151
ApaLI G'TGCA_C
Cuts at: 3427 3427
Size: 4589
ApoI r'AATT_y
Cuts at: 206 528 985 1044 1593 3942 4198 206
Size: 322 457 59 549 2349 256 597
Fragments arranged by size:
2349 597 549 457 322 256 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 4589
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
AvaI C'yCGr_G
Cuts at: 1557 1609 1557
Size: 52 4537
BamHI G'GATC_C
Cuts at: 1614 1614
Size: 4589
BanI G'GyrC_C
Cuts at: 1012 1514 1605 2019 2267 2302 1012
Size: 502 91 414 248 35 3299
Fragments arranged by size:
3299 502 414 248 91 35
BanII G_rGCy'C
Cuts at: 1097 1603 2633 1097
Size: 506 1030 3053
Fragments arranged by size:
3053 1030 506
BbeI G_GCGC'C
Cuts at: 2023 2271 2023
Size: 248 4341
BbsI GAAGACnn'nnnn_
Cuts at: 413 764 413
Size: 351 4238
BciVI GTATCCnnnnn_n'
Cuts at: 749 2487 3543 4589 749
Size: 1738 1056 1046 749
Fragments arranged by size:
1738 1056 1046 749
BclI T'GATC_A
Cuts at: 2109 2109
Size: 4589
BglI GCCn_nnn'nGGC
Cuts at: 901 901
Size: 4589
BglII A'GATC_T
Cuts at: 2104 4141 2104
Size: 2037 2552
BlpI GC'TnA_GC
Cuts at: 585 1701 585
Size: 1116 3473
Bme1580I G_kGCm'C
Cuts at: 2214 2307 3431 2214
Size: 93 1124 3372
Fragments arranged by size:
3372 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1801 1882 2217 4361 1801
Size: 81 335 2144 2029
Fragments arranged by size:
2144 2029 335 81
BmtI G_CTAG'C
Cuts at: 545 545
Size: 4589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 638 812 410
Size: 228 174 4187
Fragments arranged by size:
4187 228 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1765 2644 3358 3656 1765
Size: 879 714 298 2698
Fragments arranged by size:
2698 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4293 4293
Size: 4589
BsaAI yAC'GTr
Cuts at: 667 1942 667
Size: 1275 3314
BsaBI GATnn'nnATC
Cuts at: 6 2103 4140 6
Size: 2097 2037 455
Fragments arranged by size:
2097 2037 455
BsaHI Gr'CG_yC
Cuts at: 2020 2268 2970 4466 2020
Size: 248 702 1496 2143
Fragments arranged by size:
2143 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1778 2299 2977 3388 3535 3809 1778
Size: 521 678 411 147 274 2558
Fragments arranged by size:
2558 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 548 578 816 846 548
Size: 30 238 30 4291
Fragments arranged by size:
4291 238 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4589
BseYI C'CCAG_C
Cuts at: 2014 2772 3437 3845 2014
Size: 758 665 408 2758
Fragments arranged by size:
2758 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 111
Size: 4589
BsiEI CG_ry'CG
Cuts at: 104 2131 2177 3407 104
Size: 2027 46 1230 1286
Fragments arranged by size:
2027 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1603 2381 2571 3431 1603
Size: 778 190 860 2761
Fragments arranged by size:
2761 860 778 190
BsmI GAATG_Cn'
Cuts at: 178 1481 178
Size: 1303 3286
Bsp1286I G_dGCh'C
Cuts at: 1097 1603 2214 2307 2381 2571 2633 3431 1097
Size: 506 611 93 74 190 62 798 2255
Fragments arranged by size:
2255 798 611 506 190 93 74 62
BspHI T'CATG_A
Cuts at: 336 3021 336
Size: 2685 1904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 133 1639 1853 2155 2536 133
Size: 1506 214 302 381 2186
Fragments arranged by size:
2186 1506 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2881 2935 4419 4584 50
Size: 2831 54 1484 165 55
Fragments arranged by size:
2831 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2501 2501
Size: 4589
BsrFI r'CCGG_y
Cuts at: 2587 2768 2587
Size: 181 4408
BsrGI T'GTAC_A
Cuts at: 1404 1404
Size: 4589
BssSI C'ACGA_G
Cuts at: 1505 2860 3568 1505
Size: 1355 708 2526
Fragments arranged by size:
2526 1355 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1966 1966
Size: 4589
BstBI TT'CG_AA
Cuts at: 2950 2950
Size: 4589
BstEII G'GTnAC_C
Cuts at: 1562 1562
Size: 4589
BstXI CCAn_nnnn'nTGG
Cuts at: 749 749
Size: 4589
BstYI r'GATC_y
Cuts at: 1 672 920 1614 2081 2104 2439 2685 2991
Size: 671 248 694 467 23 335 246 306
Cuts at: 2991 3003 3089 4141 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 694 671 467 449 335 306 248
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 457 457
Size: 4589
BtgI C'CryG_G
Cuts at: 107 916 107
Size: 809 3780
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 2539 2720 2883 1537
Size: 1002 181 163 3243
Fragments arranged by size:
3243 1002 181 163
BtsI GCAGTG_nn'
Cuts at: 1476 1832 1476
Size: 356 4233
DraI TTT'AAA
Cuts at: 1588 1588
Size: 4589
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2295 3639 1491
Size: 804 1344 2441
Fragments arranged by size:
2441 1344 804
EaeI y'GGCC_r
Cuts at: 2174 2348 2739 2766 4111 2174
Size: 174 391 27 1345 2652
Fragments arranged by size:
2652 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2174 2174
Size: 4589
EarI CTCTTCn'nnn_
Cuts at: 347 1493 2612 2822 347
Size: 1146 1119 210 2114
Fragments arranged by size:
2114 1146 1119 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2797 3539 3685 4399 2797
Size: 742 146 714 2987
Fragments arranged by size:
2987 742 714 146
EcoICRI GAG'CTC
Cuts at: 1601 1601
Size: 4589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 538 638 812 857 928 1316 2414 2846
Size: 128 100 174 45 71 388 1098 432
Cuts at: 2846 3199 3861 410
Size: 353 662 1138
Fragments arranged by size:
1138 1098 662 432 388 353 174 128
100 71 45
EcoNI CCTnn'n_nnAGG
Cuts at: 129 129
Size: 4589
EcoO109I rG'GnC_Cy
Cuts at: 513 1728 513
Size: 1215 3374
EcoRI G'AATT_C
Cuts at: 1593 1593
Size: 4589
EcoRV GAT'ATC
Cuts at: 646 646
Size: 4589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4589
FspI TGC'GCA
Cuts at: 2370 2370
Size: 4589
HaeII r_GCGC'y
Cuts at: 2023 2271 3501 4272 2023
Size: 248 1230 771 2340
Fragments arranged by size:
2340 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 793 825 2431 2461 2463 2493 331
Size: 32 430 32 1606 30 2 30 2427
Fragments arranged by size:
2427 1606 430 32 32 30 30 2
HincII GTy'rAC
Cuts at: 928 1139 1628 928
Size: 211 489 3889
Fragments arranged by size:
3889 489 211
HindIII A'AGCT_T
Cuts at: 253 1298 253
Size: 1045 3544
KasI G'GCGC_C
Cuts at: 2019 2267 2019
Size: 248 4341
KpnI G_GTAC'C
Cuts at: 1609 1609
Size: 4589
MfeI C'AATT_G
Cuts at: 1160 1160
Size: 4589
MluI A'CGCG_T
Cuts at: 4022 4022
Size: 4589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3348 3532 3348
Size: 184 4405
MscI TGG'CCA
Cuts at: 2350 2350
Size: 4589
MslI CAynn'nnrTG
Cuts at: 494 816 2578 2705 494
Size: 322 1762 127 2378
Fragments arranged by size:
2378 1762 322 127
MspA1I CmG'CkG
Cuts at: 562 1700 2014 2374 3158 3403 562
Size: 1138 314 360 784 245 1748
Fragments arranged by size:
1748 1138 784 360 314 245
NaeI GCC'GGC
Cuts at: 2770 2770
Size: 4589
NarI GG'CG_CC
Cuts at: 2020 2268 2020
Size: 248 4341
NcoI C'CATG_G
Cuts at: 107 916 107
Size: 809 3780
NgoMIV G'CCGG_C
Cuts at: 2768 2768
Size: 4589
NheI G'CTAG_C
Cuts at: 541 541
Size: 4589
NspI r_CATG'y
Cuts at: 707 1642 2142 3745 707
Size: 935 500 1603 1551
Fragments arranged by size:
1603 1551 935 500
PciI A'CATG_T
Cuts at: 3741 3741
Size: 4589
PmeI GTTT'AAAC
Cuts at: 1588 1588
Size: 4589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 4557
PpuMI rG'GwC_Cy
Cuts at: 513 513
Size: 4589
PstI C_TGCA'G
Cuts at: 1636 2321 1636
Size: 685 3904
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4589
PvuII CAG'CTG
Cuts at: 562 2014 2374 562
Size: 1452 360 2777
Fragments arranged by size:
2777 1452 360
RsrII CG'GwC_CG
Cuts at: 2784 2784
Size: 4589
SacI G_AGCT'C
Cuts at: 1603 1603
Size: 4589
SalI G'TCGA_C
Cuts at: 1626 1626
Size: 4589
SapI GCTCTTCn'nnn_
Cuts at: 2612 2822 2612
Size: 210 4379
SbfI CC_TGCA'GG
Cuts at: 1636 1636
Size: 4589
ScaI AGT'ACT
Cuts at: 4106 4106
Size: 4589
SexAI A'CCwGG_T
Cuts at: 130 130
Size: 4589
SfcI C'TryA_G
Cuts at: 33 429 968 1632 2317 3285 3476 33
Size: 396 539 664 685 968 191 1146
Fragments arranged by size:
1146 968 685 664 539 396 191
SfoI GGC'GCC
Cuts at: 2021 2269 2021
Size: 248 4341
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
SmaI CCC'GGG
Cuts at: 1611 1611
Size: 4589
SmlI C'TyrA_G
Cuts at: 1744 2659 3373 3635 1744
Size: 915 714 262 2698
Fragments arranged by size:
2698 915 714 262
SnaBI TAC'GTA
Cuts at: 667 667
Size: 4589
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4589
SphI G_CATG'C
Cuts at: 1642 2142 1642
Size: 500 4089
SspI AAT'ATT
Cuts at: 324 324
Size: 4589
StuI AGG'CCT
Cuts at: 1277 1277
Size: 4589
StyI C'CwwG_G
Cuts at: 107 551 916 1723 2077 107
Size: 444 365 807 354 2619
Fragments arranged by size:
2619 807 444 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 929 2172 2305 2764 2973 929
Size: 1243 133 459 209 2545
Fragments arranged by size:
2545 1243 459 209 133
TatI w'GTAC_w
Cuts at: 1404 1457 4104 1404
Size: 53 2647 1889
Fragments arranged by size:
2647 1889 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 548 580 674 706 816 848 1516 1548 4106
Size: 32 94 32 110 32 668 32 2558
Cuts at: 4106 4138 548
Size: 32 999
Fragments arranged by size:
2558 999 668 110 94 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2386 2386
Size: 4589
XbaI T'CTAG_A
Cuts at: 468 1620 468
Size: 1152 3437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 695 815 1120 2084 695
Size: 120 305 964 3200
Fragments arranged by size:
3200 964 305 120
XmaI C'CCGG_G
Cuts at: 1609 1609
Size: 4589
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
Bpu10I BsiWI BsmBI BspEI BssHII Bsu36I ClaI CspCI
DraIII FseI FspAI HpaI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SwaI
XhoI XmnI ZraI