Restriction map for
pF1KB4161
(Circular) (Six-base) MAPSORT of: pF1KB4161.seq Check: 5561 from: 1 to: 4526
pF1KB4161 4526 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 975 975
Size: 4526
AccI GT'mk_AC
Cuts at: 322 1564 322
Size: 1242 3284
Acc65I G'GTAC_C
Cuts at: 1542 1542
Size: 4526
AclI AA'CG_TT
Cuts at: 4366 4366
Size: 4526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2783 3530 3798 2351
Size: 432 747 268 3079
Fragments arranged by size:
3079 747 432 268
AflII C'TTAA_G
Cuts at: 1294 1294
Size: 4526
AflIII A'CryG_T
Cuts at: 1878 2982 3959 1878
Size: 1104 977 2445
Fragments arranged by size:
2445 1104 977
AgeI A'CCGG_T
Cuts at: 2914 2914
Size: 4526
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 154 186 154
Size: 32 4494
AlwNI CAG_nnn'CTG
Cuts at: 399 600 1177 1831 3398 399
Size: 201 577 654 1567 1527
Fragments arranged by size:
1567 1527 654 577 201
ApaI G_GGCC'C
Cuts at: 675 1087 675
Size: 412 4114
ApaLI G'TGCA_C
Cuts at: 3296 3296
Size: 4526
ApoI r'AATT_y
Cuts at: 448 1114 1530 3879 4135 448
Size: 666 416 2349 256 839
Fragments arranged by size:
2349 839 666 416 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
AvaI C'yCGr_G
Cuts at: 680 1250 1376 1400 1546 680
Size: 570 126 24 146 3660
Fragments arranged by size:
3660 570 146 126 24
BamHI G'GATC_C
Cuts at: 1551 1551
Size: 4526
BanI G'GyrC_C
Cuts at: 1339 1418 1514 1542 1956 2204 2239 1339
Size: 79 96 28 414 248 35 3626
Fragments arranged by size:
3626 414 248 96 79 35 28
BanII G_rGCy'C
Cuts at: 675 1087 1354 1540 2570 675
Size: 412 267 186 1030 2631
Fragments arranged by size:
2631 1030 412 267 186
BbeI G_GCGC'C
Cuts at: 1960 2208 1960
Size: 248 4278
BbsI GAAGACnn'nnnn_
Cuts at: 1297 1297
Size: 4526
BbvCI CC'TCA_GC
Cuts at: 491 491
Size: 4526
BciVI GTATCCnnnnn_n'
Cuts at: 898 2424 3185 4526 898
Size: 1526 761 1341 898
Fragments arranged by size:
1526 1341 898 761
BclI T'GATC_A
Cuts at: 2046 2046
Size: 4526
BglII A'GATC_T
Cuts at: 2041 4078 2041
Size: 2037 2489
BlpI GC'TnA_GC
Cuts at: 1638 1638
Size: 4526
Bme1580I G_kGCm'C
Cuts at: 675 1087 2151 2244 3300 675
Size: 412 1064 93 1056 1901
Fragments arranged by size:
1901 1064 1056 412 93
BmgBI CAC'GTC
Cuts at: 576 576
Size: 4526
BmrI ACTGGGnnnn_n'
Cuts at: 758 1738 1819 2154 4298 758
Size: 980 81 335 2144 986
Fragments arranged by size:
2144 986 980 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 4526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1199
Size: 4526
Bpu10I CC'TnA_GC
Cuts at: 491 491
Size: 4526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 840 1702 2581 3073 3371 840
Size: 862 879 492 298 1995
Fragments arranged by size:
1995 879 862 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4230 4230
Size: 4526
BsaAI yAC'GTr
Cuts at: 1879 1879
Size: 4526
BsaBI GATnn'nnATC
Cuts at: 6 2040 4077 6
Size: 2034 2037 455
Fragments arranged by size:
2037 2034 455
BsaHI Gr'CG_yC
Cuts at: 1957 2205 2907 4403 1957
Size: 248 702 1496 2080
Fragments arranged by size:
2080 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1715 2236 2914 3188 3335 3746 1715
Size: 521 678 274 147 411 2495
Fragments arranged by size:
2495 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 554 584 554
Size: 30 4496
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 254 843 863 866 869 908 920 1278
Size: 91 589 20 3 3 39 12 358
Cuts at: 1278 163
Size: 3411
Fragments arranged by size:
3411 589 358 91 39 20 12 3
3
BseYI C'CCAG_C
Cuts at: 1951 2709 3286 3782 1951
Size: 758 577 496 2695
Fragments arranged by size:
2695 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1208
Size: 4526
BsiEI CG_ry'CG
Cuts at: 104 2068 2114 3322 104
Size: 1964 46 1208 1308
Fragments arranged by size:
1964 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1354 1540 2318 2508 3300 1354
Size: 186 778 190 792 2580
Fragments arranged by size:
2580 792 778 190 186
BsmBI CGTCTCn'nnnn_
Cuts at: 1207 1207
Size: 4526
Bsp1286I G_dGCh'C
Cuts at: 675 1087 1354 1540 2151 2244 2318 2508 2570
Size: 412 267 186 611 93 74 190 62
Cuts at: 2570 3300 675
Size: 730 1901
Fragments arranged by size:
1901 730 611 412 267 190 186 93
74 62
BspEI T'CCGG_A
Cuts at: 3746 3746
Size: 4526
BspHI T'CATG_A
Cuts at: 1127 3702 1127
Size: 2575 1951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 975 1576 1790 2092 2473 975
Size: 601 214 302 381 3028
Fragments arranged by size:
3028 601 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2818 2872 4356 4521 50
Size: 2768 54 1484 165 55
Fragments arranged by size:
2768 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1229 2438 1229
Size: 1209 3317
BsrFI r'CCGG_y
Cuts at: 582 1394 1511 2524 2705 2914 582
Size: 812 117 1013 181 209 2194
Fragments arranged by size:
2194 1013 812 209 181 117
BssSI C'ACGA_G
Cuts at: 746 2797 3155 746
Size: 2051 358 2117
Fragments arranged by size:
2117 2051 358
BstAPI GCAn_nnn'nTGC
Cuts at: 906 1903 906
Size: 997 3529
BstBI TT'CG_AA
Cuts at: 2887 2887
Size: 4526
BstEII G'GTnAC_C
Cuts at: 1385 1385
Size: 4526
BstYI r'GATC_y
Cuts at: 1 1425 1551 2018 2041 2376 2622 3634 3720
Size: 1424 126 467 23 335 246 1012 86
Cuts at: 3720 3732 4078 1
Size: 12 346 449
Fragments arranged by size:
1424 1012 467 449 346 335 246 126
86 23 12
BstZ17I GTA'TAC
Cuts at: 323 323
Size: 4526
BtgI C'CryG_G
Cuts at: 667 1065 667
Size: 398 4128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1354 2476 2657 2820 1354
Size: 1122 181 163 3060
Fragments arranged by size:
3060 1122 181 163
BtsI GCAGTG_nn'
Cuts at: 1161 1769 1161
Size: 608 3918
DraI TTT'AAA
Cuts at: 1525 1525
Size: 4526
DrdI GACnn_nn'nnGTC
Cuts at: 2232 3090 2232
Size: 858 3668
EaeI y'GGCC_r
Cuts at: 471 584 2111 2285 2676 2703 4048 471
Size: 113 1527 174 391 27 1345 949
Fragments arranged by size:
1527 1345 949 391 174 113 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4526
EarI CTCTTCn'nnn_
Cuts at: 1359 2549 2759 1359
Size: 1190 210 3126
Fragments arranged by size:
3126 1190 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2734 3044 3190 4336 2734
Size: 310 146 1146 2924
Fragments arranged by size:
2924 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1352 1538 1352
Size: 186 4340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 2351 2783 3530 3798 1199
Size: 1152 432 747 268 1927
Fragments arranged by size:
1927 1152 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 162 867 1018 1084 1665 162
Size: 705 151 66 581 3023
Fragments arranged by size:
3023 705 581 151 66
EcoRI G'AATT_C
Cuts at: 1530 1530
Size: 4526
EcoRV GAT'ATC
Cuts at: 1125 1125
Size: 4526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4526
FseI GG_CCGG'CC
Cuts at: 586 586
Size: 4526
FspI TGC'GCA
Cuts at: 2307 2307
Size: 4526
HaeII r_GCGC'y
Cuts at: 1960 2208 3230 4209 1960
Size: 248 1022 979 2277
Fragments arranged by size:
2277 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2368 2398 2400 2430 2368
Size: 30 2 30 4464
Fragments arranged by size:
4464 30 30 2
HincII GTy'rAC
Cuts at: 1565 1565
Size: 4526
HindIII A'AGCT_T
Cuts at: 858 858
Size: 4526
KasI G'GCGC_C
Cuts at: 1956 2204 1956
Size: 248 4278
KpnI G_GTAC'C
Cuts at: 1546 1546
Size: 4526
MfeI C'AATT_G
Cuts at: 1208 1208
Size: 4526
MluI A'CGCG_T
Cuts at: 3959 3959
Size: 4526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 3197 3381 512
Size: 2685 184 1657
Fragments arranged by size:
2685 1657 184
MscI TGG'CCA
Cuts at: 473 2287 473
Size: 1814 2712
MslI CAynn'nnrTG
Cuts at: 2515 2642 2515
Size: 127 4399
MspA1I CmG'CkG
Cuts at: 399 1637 1951 2311 3324 3569 399
Size: 1238 314 360 1013 245 1356
Fragments arranged by size:
1356 1238 1013 360 314 245
NaeI GCC'GGC
Cuts at: 584 2707 584
Size: 2123 2403
NarI GG'CG_CC
Cuts at: 1957 2205 1957
Size: 248 4278
NcoI C'CATG_G
Cuts at: 1065 1065
Size: 4526
NgoMIV G'CCGG_C
Cuts at: 582 2705 582
Size: 2123 2403
NspI r_CATG'y
Cuts at: 1579 2079 2986 1579
Size: 500 907 3119
Fragments arranged by size:
3119 907 500
PasI CC'CwG_GG
Cuts at: 952 952
Size: 4526
PciI A'CATG_T
Cuts at: 2982 2982
Size: 4526
PfoI T'CCnGG_A
Cuts at: 1357 1357
Size: 4526
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4494
PpuMI rG'GwC_Cy
Cuts at: 162 867 162
Size: 705 3821
PspOMI G'GGCC_C
Cuts at: 671 1083 671
Size: 412 4114
PstI C_TGCA'G
Cuts at: 1573 2258 1573
Size: 685 3841
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4526
PvuII CAG'CTG
Cuts at: 399 1951 2311 399
Size: 1552 360 2614
Fragments arranged by size:
2614 1552 360
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4526
SacI G_AGCT'C
Cuts at: 1354 1540 1354
Size: 186 4340
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4526
SanDI GG'GwC_CC
Cuts at: 867 867
Size: 4526
SapI GCTCTTCn'nnn_
Cuts at: 1359 2549 2759 1359
Size: 1190 210 3126
Fragments arranged by size:
3126 1190 210
SbfI CC_TGCA'GG
Cuts at: 1573 1573
Size: 4526
ScaI AGT'ACT
Cuts at: 4043 4043
Size: 4526
SfcI C'TryA_G
Cuts at: 33 327 419 1010 1097 1329 1569 2254 3247
Size: 294 92 591 87 232 240 685 993
Cuts at: 3247 3438 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 591 294 240 232 191
92 87
SfoI GGC'GCC
Cuts at: 1958 2206 1958
Size: 248 4278
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
SmaI CCC'GGG
Cuts at: 1252 1378 1402 1548 1252
Size: 126 24 146 4230
Fragments arranged by size:
4230 146 126 24
SmlI C'TyrA_G
Cuts at: 855 1294 1681 2596 3088 3350 855
Size: 439 387 915 492 262 2031
Fragments arranged by size:
2031 915 492 439 387 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4526
SphI G_CATG'C
Cuts at: 1579 2079 1579
Size: 500 4026
StuI AGG'CCT
Cuts at: 1045 1045
Size: 4526
StyI C'CwwG_G
Cuts at: 1065 1660 2014 1065
Size: 595 354 3577
Fragments arranged by size:
3577 595 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1327 2109 2242 2701 2910 1327
Size: 782 133 459 209 2943
Fragments arranged by size:
2943 782 459 209 133
TatI w'GTAC_w
Cuts at: 4041 4041
Size: 4526
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 554 586 4043 4075 554
Size: 32 3457 32 1005
Fragments arranged by size:
3457 1005 32 32
Tth111I GACn'n_nGTC
Cuts at: 2323 2323
Size: 4526
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2021 2021
Size: 4526
XmaI C'CCGG_G
Cuts at: 1250 1376 1400 1546 1250
Size: 126 24 146 4230
Fragments arranged by size:
4230 146 126 24
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsiWI BsmI BsrGI BssHII
BstXI Bsu36I ClaI CspCI DraIII EcoNI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI