Restriction map for
pF1KB4165
(Circular) (Six-base) MAPSORT of: pF1KB4165.seq Check: 9488 from: 1 to: 5591
pF1KB4165 5591 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 951 959 969 1260 1556 951
Size: 8 10 291 296 4986
Fragments arranged by size:
4986 296 291 10 8
AatII G_ACGT'C
Cuts at: 2228 2228
Size: 5591
AccI GT'mk_AC
Cuts at: 2629 2629
Size: 5591
Acc65I G'GTAC_C
Cuts at: 2607 2607
Size: 5591
AclI AA'CG_TT
Cuts at: 5431 5431
Size: 5591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1006 3416 3848 4201 4863 454
Size: 552 2410 432 353 662 1182
Fragments arranged by size:
2410 1182 662 552 432 353
AfeI AGC'GCT
Cuts at: 1827 1827
Size: 5591
AflIII A'CryG_T
Cuts at: 1619 2943 4743 5024 1619
Size: 1324 1800 281 2186
Fragments arranged by size:
2186 1800 1324 281
AhdI GACnn_n'nnGTC
Cuts at: 657 657
Size: 5591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 624 712 2194 624
Size: 88 1482 4021
Fragments arranged by size:
4021 1482 88
AlwNI CAG_nnn'CTG
Cuts at: 951 984 1005 1092 1110 1368 1641 2896 4334
Size: 33 21 87 18 258 273 1255 1438
Cuts at: 4334 951
Size: 2208
Fragments arranged by size:
2208 1438 1255 273 258 87 33 21
18
ApaI G_GGCC'C
Cuts at: 183 855 1080 1761 2517 183
Size: 672 225 681 756 3257
Fragments arranged by size:
3257 756 681 672 225
ApaLI G'TGCA_C
Cuts at: 1177 4429 1177
Size: 3252 2339
ApoI r'AATT_y
Cuts at: 2595 4944 5200 2595
Size: 2349 256 2986
Fragments arranged by size:
2986 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5591
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5591
AvaI C'yCGr_G
Cuts at: 190 261 2611 190
Size: 71 2350 3170
Fragments arranged by size:
3170 2350 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 284 317 1728 1761 2326 2359 284
Size: 33 1411 33 565 33 3516
Fragments arranged by size:
3516 1411 565 33 33 33
BamHI G'GATC_C
Cuts at: 2554 2616 2554
Size: 62 5529
BanI G'GyrC_C
Cuts at: 493 812 1699 2607 3021 3269 3304 493
Size: 319 887 908 414 248 35 2780
Fragments arranged by size:
2780 908 887 414 319 248 35
BanII G_rGCy'C
Cuts at: 183 191 366 407 855 1015 1080 1158 1262
Size: 8 175 41 448 160 65 78 104
Cuts at: 1262 1424 1761 2517 2605 3635 183
Size: 162 337 756 88 1030 2139
Fragments arranged by size:
2139 1030 756 448 337 175 162 160
104 88 78 65 41 8
BbeI G_GCGC'C
Cuts at: 816 1703 3025 3273 816
Size: 887 1322 248 3134
Fragments arranged by size:
3134 1322 887 248
BbsI GAAGACnn'nnnn_
Cuts at: 736 1584 736
Size: 848 4743
BbvCI CC'TCA_GC
Cuts at: 746 922 746
Size: 176 5415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 820 854 820
Size: 34 5557
BciVI GTATCCnnnnn_n'
Cuts at: 3489 4545 5591 3489
Size: 1056 1046 3489
Fragments arranged by size:
3489 1056 1046
BclI T'GATC_A
Cuts at: 3111 3111
Size: 5591
BglI GCCn_nnn'nGGC
Cuts at: 232 1697 1910 232
Size: 1465 213 3913
Fragments arranged by size:
3913 1465 213
BglII A'GATC_T
Cuts at: 1835 3106 5143 1835
Size: 1271 2037 2283
Fragments arranged by size:
2283 2037 1271
BlpI GC'TnA_GC
Cuts at: 2703 2703
Size: 5591
Bme1580I G_kGCm'C
Cuts at: 183 327 496 855 1080 1181 1761 1906 2264
Size: 144 169 359 225 101 580 145 358
Cuts at: 2264 2517 3216 3309 4433 183
Size: 253 699 93 1124 1341
Fragments arranged by size:
1341 1124 699 580 359 358 253 225
169 145 144 101 93
BmgBI CAC'GTC
Cuts at: 128 1620 128
Size: 1492 4099
BmrI ACTGGGnnnn_n'
Cuts at: 287 292 387 849 1812 2803 2884 3219 5363
Size: 5 95 462 963 991 81 335 2144
Cuts at: 5363 287
Size: 515
Fragments arranged by size:
2144 991 963 515 462 335 95 81
5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2055 3958 2055
Size: 1903 3688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 694 1090 1114 1909 303
Size: 391 396 24 795 3985
Fragments arranged by size:
3985 795 396 391 24
Bpu10I CC'TnA_GC
Cuts at: 367 746 922 367
Size: 379 176 5036
Fragments arranged by size:
5036 379 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1246 2368 2767 3646 4360 4658 748
Size: 498 1122 399 879 714 298 1681
Fragments arranged by size:
1681 1122 879 714 498 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 826 5295 826
Size: 4469 1122
BsaAI yAC'GTr
Cuts at: 2259 2944 2259
Size: 685 4906
BsaBI GATnn'nnATC
Cuts at: 6 3105 5142 6
Size: 3099 2037 455
Fragments arranged by size:
3099 2037 455
BsaHI Gr'CG_yC
Cuts at: 813 1196 1700 1795 2225 2494 3022 3270 3972
Size: 383 504 95 430 269 528 248 702
Cuts at: 3972 5468 813
Size: 1496 936
Fragments arranged by size:
1496 936 702 528 504 430 383 269
248 95
BsaWI w'CCGG_w
Cuts at: 1199 2561 2780 3301 3979 4390 4537 4811 1199
Size: 1362 219 521 678 411 147 274 1979
Fragments arranged by size:
1979 1362 678 521 411 274 219 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 883 913 1090 1120 1519 1549 1780
Size: 30 552 30 177 30 399 30 231
Cuts at: 1780 1810 301
Size: 30 4082
Fragments arranged by size:
4082 552 399 231 177 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 808 811 1111 1153 1357 808
Size: 3 300 42 204 5042
Fragments arranged by size:
5042 300 204 42 3
BseYI C'CCAG_C
Cuts at: 216 222 342 486 641 662 2415 2527 3016
Size: 6 120 144 155 21 1753 112 489
Cuts at: 3016 3774 4439 4847 216
Size: 758 665 408 960
Fragments arranged by size:
1753 960 758 665 489 408 155 144
120 112 21 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 742 1537 1654 1804 1975 353
Size: 389 795 117 150 171 3969
Fragments arranged by size:
3969 795 389 171 150 117
BsiEI CG_ry'CG
Cuts at: 104 236 738 2012 2585 3133 3179 4409 104
Size: 132 502 1274 573 548 46 1230 1286
Fragments arranged by size:
1286 1274 1230 573 548 502 132 46
BsiHKAI G_wGCw'C
Cuts at: 626 1015 1181 1424 2036 2605 3383 3573 4433
Size: 389 166 243 612 569 778 190 860
Cuts at: 4433 626
Size: 1784
Fragments arranged by size:
1784 860 778 612 569 389 243 190
166
BsiWI C'GTAC_G
Cuts at: 1685 1685
Size: 5591
BsmBI CGTCTCn'nnnn_
Cuts at: 2362 2362
Size: 5591
Bsp1286I G_dGCh'C
Cuts at: 183 191 327 366 407 496 626 855 1015
Size: 8 136 39 41 89 130 229 160
Cuts at: 1015 1080 1158 1181 1262 1424 1761 1906 2036
Size: 65 78 23 81 162 337 145 130
Cuts at: 2036 2264 2517 2605 3216 3309 3383 3573 3635
Size: 228 253 88 611 93 74 190 62
Cuts at: 3635 4433 183
Size: 798 1341
Fragments arranged by size:
1341 798 611 337 253 229 228 190
162 160 145 136 130 130 93 89
88 81 78 74 65 62 41 39
23 8
BspEI T'CCGG_A
Cuts at: 1199 2561 1199
Size: 1362 4229
BspHI T'CATG_A
Cuts at: 4023 4023
Size: 5591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 411 527 592 951 959 969 1260 1556 1868
Size: 116 65 359 8 10 291 296 312
Cuts at: 1868 2297 2641 2855 3157 3538 411
Size: 429 344 214 302 381 2464
Fragments arranged by size:
2464 429 381 359 344 312 302 296
291 214 116 65 10 8
BsrBI CCG'CTC
Cuts at: 50 801 1074 2045 3883 3937 5421 5586 50
Size: 751 273 971 1838 54 1484 165 55
Fragments arranged by size:
1838 1484 971 751 273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 836 2228 3503 836
Size: 1392 1275 2924
Fragments arranged by size:
2924 1392 1275
BsrFI r'CCGG_y
Cuts at: 256 737 1224 1702 2481 3589 3770 256
Size: 481 487 478 779 1108 181 2077
Fragments arranged by size:
2077 1108 779 487 481 478 181
BssSI C'ACGA_G
Cuts at: 829 1180 1423 1457 3862 4570 829
Size: 351 243 34 2405 708 1850
Fragments arranged by size:
2405 1850 708 351 243 34
BstAPI GCAn_nnn'nTGC
Cuts at: 555 585 2055 2968 555
Size: 30 1470 913 3178
Fragments arranged by size:
3178 1470 913 30
BstBI TT'CG_AA
Cuts at: 3952 3952
Size: 5591
BstEII G'GTnAC_C
Cuts at: 1644 1644
Size: 5591
BstYI r'GATC_y
Cuts at: 1 1835 1892 2554 2616 3083 3106 3441 3687
Size: 1834 57 662 62 467 23 335 246
Cuts at: 3687 3993 4005 4091 5143 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1834 1052 662 467 449 335 306 246
86 62 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 1306 1496 1852 1877 1306
Size: 190 356 25 5020
Fragments arranged by size:
5020 356 190 25
BtgI C'CryG_G
Cuts at: 107 399 1080 2105 2348 107
Size: 292 681 1025 243 3350
Fragments arranged by size:
3350 1025 681 292 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3541 3722 3885 3541
Size: 181 163 5247
Fragments arranged by size:
5247 181 163
BtsI GCAGTG_nn'
Cuts at: 1228 1285 1920 2834 1228
Size: 57 635 914 3985
Fragments arranged by size:
3985 914 635 57
DraI TTT'AAA
Cuts at: 2590 2590
Size: 5591
DrdI GACnn_nn'nnGTC
Cuts at: 3297 4641 3297
Size: 1344 4247
EaeI y'GGCC_r
Cuts at: 233 1465 1689 1992 2582 3176 3350 3741 3768
Size: 1232 224 303 590 594 174 391 27
Cuts at: 3768 5113 233
Size: 1345 711
Fragments arranged by size:
1345 1232 711 594 590 391 303 224
174 27
EagI C'GGCC_G
Cuts at: 233 2582 3176 233
Size: 2349 594 2648
Fragments arranged by size:
2648 2349 594
EarI CTCTTCn'nnn_
Cuts at: 1109 1777 3614 3824 1109
Size: 668 1837 210 2876
Fragments arranged by size:
2876 1837 668 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 697 3799 4541 4687 5401 697
Size: 3102 742 146 714 887
Fragments arranged by size:
3102 887 742 714 146
EcoICRI GAG'CTC
Cuts at: 1013 1422 2603 1013
Size: 409 1181 4001
Fragments arranged by size:
4001 1181 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 454 694 1006 1090 1114 1909 3416 3848
Size: 151 240 312 84 24 795 1507 432
Cuts at: 3848 4201 4863 303
Size: 353 662 1031
Fragments arranged by size:
1507 1031 795 662 432 353 312 240
151 84 24
EcoNI CCTnn'n_nnAGG
Cuts at: 120 1114 2182 2293 120
Size: 994 1068 111 3418
Fragments arranged by size:
3418 1068 994 111
EcoO109I rG'GnC_Cy
Cuts at: 159 180 516 604 918 1302 1492 1638 1758
Size: 21 336 88 314 384 190 146 120
Cuts at: 1758 1848 1857 2419 2447 2730 159
Size: 90 9 562 28 283 3020
Fragments arranged by size:
3020 562 384 336 314 283 190 146
120 90 88 28 21 9
EcoRI G'AATT_C
Cuts at: 2595 2595
Size: 5591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3610 3610
Size: 5591
FspI TGC'GCA
Cuts at: 589 1375 3372 589
Size: 786 1997 2808
Fragments arranged by size:
2808 1997 786
HaeII r_GCGC'y
Cuts at: 564 641 816 1703 1829 3025 3273 4503 5274
Size: 77 175 887 126 1196 248 1230 771
Cuts at: 5274 564
Size: 881
Fragments arranged by size:
1230 1196 887 881 771 248 175 126
77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 643 675 883 915 1090 1122 1519
Size: 32 310 32 208 32 175 32 397
Cuts at: 1519 1551 1780 1812 2431 2463 3433 3463 3465
Size: 32 229 32 619 32 970 30 2
Cuts at: 3465 3495 301
Size: 30 2397
Fragments arranged by size:
2397 970 619 397 310 229 208 175
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1350 2630 1350
Size: 1280 4311
KasI G'GCGC_C
Cuts at: 812 1699 3021 3269 812
Size: 887 1322 248 3134
Fragments arranged by size:
3134 1322 887 248
KpnI G_GTAC'C
Cuts at: 2611 2611
Size: 5591
MluI A'CGCG_T
Cuts at: 5024 5024
Size: 5591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4350 4534 4350
Size: 184 5407
MscI TGG'CCA
Cuts at: 1994 3352 1994
Size: 1358 4233
MslI CAynn'nnrTG
Cuts at: 1447 3580 3707 1447
Size: 2133 127 3331
Fragments arranged by size:
3331 2133 127
MspA1I CmG'CkG
Cuts at: 666 672 1005 1023 1398 1732 1755 1767 1923
Size: 6 333 18 375 334 23 12 156
Cuts at: 1923 2702 3016 3376 4160 4405 666
Size: 779 314 360 784 245 1852
Fragments arranged by size:
1852 784 779 375 360 334 333 314
245 156 23 18 12 6
NaeI GCC'GGC
Cuts at: 1226 1704 2483 3772 1226
Size: 478 779 1289 3045
Fragments arranged by size:
3045 1289 779 478
NarI GG'CG_CC
Cuts at: 813 1700 3022 3270 813
Size: 887 1322 248 3134
Fragments arranged by size:
3134 1322 887 248
NcoI C'CATG_G
Cuts at: 107 1080 107
Size: 973 4618
NgoMIV G'CCGG_C
Cuts at: 1224 1702 2481 3770 1224
Size: 478 779 1289 3045
Fragments arranged by size:
3045 1289 779 478
NspI r_CATG'y
Cuts at: 699 2644 3144 4747 699
Size: 1945 500 1603 1543
Fragments arranged by size:
1945 1603 1543 500
PciI A'CATG_T
Cuts at: 4743 4743
Size: 5591
PfoI T'CCnGG_A
Cuts at: 1625 1625
Size: 5591
PmeI GTTT'AAAC
Cuts at: 2590 2590
Size: 5591
PmlI CAC'GTG
Cuts at: 2259 2259
Size: 5591
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 5559
PpuMI rG'GwC_Cy
Cuts at: 516 604 1492 1848 1857 2447 516
Size: 88 888 356 9 590 3660
Fragments arranged by size:
3660 888 590 356 88 9
PspOMI G'GGCC_C
Cuts at: 179 851 1076 1757 2513 179
Size: 672 225 681 756 3257
Fragments arranged by size:
3257 756 681 672 225
PstI C_TGCA'G
Cuts at: 422 671 956 1010 1298 1397 1766 2089 2297
Size: 249 285 54 288 99 369 323 208
Cuts at: 2297 2576 2638 3323 422
Size: 279 62 685 2690
Fragments arranged by size:
2690 685 369 323 288 285 279 249
208 99 62 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5591
PvuII CAG'CTG
Cuts at: 666 672 1005 1023 1398 1767 1923 3016 3376
Size: 6 333 18 375 369 156 1093 360
Cuts at: 3376 666
Size: 2881
Fragments arranged by size:
2881 1093 375 369 360 333 156 18
6
RsrII CG'GwC_CG
Cuts at: 3786 3786
Size: 5591
SacI G_AGCT'C
Cuts at: 1015 1424 2605 1015
Size: 409 1181 4001
Fragments arranged by size:
4001 1181 409
SalI G'TCGA_C
Cuts at: 2628 2628
Size: 5591
SapI GCTCTTCn'nnn_
Cuts at: 1777 3614 3824 1777
Size: 1837 210 3544
Fragments arranged by size:
3544 1837 210
SbfI CC_TGCA'GG
Cuts at: 422 956 2638 422
Size: 534 1682 3375
Fragments arranged by size:
3375 1682 534
ScaI AGT'ACT
Cuts at: 5108 5108
Size: 5591
SexAI A'CCwGG_T
Cuts at: 2120 2120
Size: 5591
SfcI C'TryA_G
Cuts at: 33 418 667 952 1006 1294 1393 1762 2085
Size: 385 249 285 54 288 99 369 323
Cuts at: 2085 2293 2572 2634 3319 4287 4478 33
Size: 208 279 62 685 968 191 1146
Fragments arranged by size:
1146 968 685 385 369 323 288 285
279 249 208 191 99 62 54
SfoI GGC'GCC
Cuts at: 814 1701 3023 3271 814
Size: 887 1322 248 3134
Fragments arranged by size:
3134 1322 887 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5591
SmaI CCC'GGG
Cuts at: 263 2613 263
Size: 2350 3241
SmlI C'TyrA_G
Cuts at: 763 1261 2383 2746 3661 4375 4637 763
Size: 498 1122 363 915 714 262 1717
Fragments arranged by size:
1717 1122 915 714 498 363 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5591
SphI G_CATG'C
Cuts at: 699 2644 3144 699
Size: 1945 500 3146
Fragments arranged by size:
3146 1945 500
StuI AGG'CCT
Cuts at: 1144 1606 2096 1144
Size: 462 490 4639
Fragments arranged by size:
4639 490 462
StyI C'CwwG_G
Cuts at: 107 1080 2725 3079 107
Size: 973 1645 354 2619
Fragments arranged by size:
2619 1645 973 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 748 2026 3174 3307 3766 3975 748
Size: 1278 1148 133 459 209 2364
Fragments arranged by size:
2364 1278 1148 459 209 133
TatI w'GTAC_w
Cuts at: 1808 5106 1808
Size: 3298 2293
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5108 5140 5108
Size: 32 5559
Tth111I GACn'n_nGTC
Cuts at: 1588 3388 1588
Size: 1800 3791
XbaI T'CTAG_A
Cuts at: 2469 2622 2469
Size: 153 5438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1158 3086 1158
Size: 1928 3663
XmaI C'CCGG_G
Cuts at: 261 2611 261
Size: 2350 3241
ZraI GAC'GTC
Cuts at: 2226 2226
Size: 5591
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AleI AloI AscI AvrII BfrBI BmtI
BsmI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI