Restriction map for
pF1KB4166
(Circular) (Six-base) MAPSORT of: pF1KB4166.seq Check: 4361 from: 1 to: 4295
pF1KB4166 4295 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 131 1333 131
Size: 1202 3093
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2552 3299 3567 2120
Size: 432 747 268 2848
Fragments arranged by size:
2848 747 432 268
AflIII A'CryG_T
Cuts at: 437 1647 2751 3728 437
Size: 1210 1104 977 1004
Fragments arranged by size:
1210 1104 1004 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 884 884
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 1096 1600 3167 1096
Size: 504 1567 2224
Fragments arranged by size:
2224 1567 504
ApaI G_GGCC'C
Cuts at: 1218 1268 1218
Size: 50 4245
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 1299 3648 3904 1299
Size: 2349 256 1690
Fragments arranged by size:
2349 1690 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 113 1239 1315 113
Size: 1126 76 3093
Fragments arranged by size:
3093 1126 76
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 532 1123 1153 1183 1311 1725 1973 2008 532
Size: 591 30 30 128 414 248 35 2819
Fragments arranged by size:
2819 591 414 248 128 35 30 30
BanII G_rGCy'C
Cuts at: 1218 1268 1309 2339 1218
Size: 50 41 1030 3174
Fragments arranged by size:
3174 1030 50 41
BbeI G_GCGC'C
Cuts at: 536 1729 1977 536
Size: 1193 248 2854
Fragments arranged by size:
2854 1193 248
BbvCI CC'TCA_GC
Cuts at: 140 1013 140
Size: 873 3422
BciVI GTATCCnnnnn_n'
Cuts at: 1039 2193 2954 4295 1039
Size: 1154 761 1341 1039
Fragments arranged by size:
1341 1154 1039 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 644 989 1810 3847 644
Size: 345 821 2037 1092
Fragments arranged by size:
2037 1092 821 345
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 868 1156 1186 1218 1268 1920 2013 3069 868
Size: 288 30 32 50 652 93 1056 2094
Fragments arranged by size:
2094 1056 652 288 93 50 32 30
BmgBI CAC'GTC
Cuts at: 243 243
Size: 4295
BmrI ACTGGGnnnn_n'
Cuts at: 639 1507 1588 1923 4067 639
Size: 868 81 335 2144 867
Fragments arranged by size:
2144 868 867 335 81
BmtI G_CTAG'C
Cuts at: 1064 1064
Size: 4295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 942 2662 942
Size: 1720 2575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 838 497
Size: 341 3954
Bpu10I CC'TnA_GC
Cuts at: 140 1013 140
Size: 873 3422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1471 2350 2842 3140 943
Size: 528 879 492 298 2098
Fragments arranged by size:
2098 879 528 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 1287 3999 163
Size: 1124 2712 459
Fragments arranged by size:
2712 1124 459
BsaAI yAC'GTr
Cuts at: 387 1648 387
Size: 1261 3034
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 392 533 1726 1974 2676 4172 392
Size: 141 1193 248 702 1496 515
Fragments arranged by size:
1496 1193 702 515 248 141
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 946 946
Size: 4295
BseYI C'CCAG_C
Cuts at: 365 995 1249 1720 2478 3055 3551 365
Size: 630 254 471 758 577 496 1109
Fragments arranged by size:
1109 758 630 577 496 471 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1054 763
Size: 291 4004
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3069 1309
Size: 778 190 792 2535
Fragments arranged by size:
2535 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 868 1156 1186 1218 1268 1309 1920 2013 2087
Size: 288 30 32 50 41 611 93 74
Cuts at: 2087 2277 2339 3069 868
Size: 190 62 730 2094
Fragments arranged by size:
2094 730 611 288 190 93 74 62
50 41 32 30
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 328 785 1345 1559 1861 2242 328
Size: 457 560 214 302 381 2381
Fragments arranged by size:
2381 560 457 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1136 1166 1196 2587 2641 4125 4290 50
Size: 1086 30 30 1391 54 1484 165 55
Fragments arranged by size:
1484 1391 1086 165 55 54 30 30
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 178 272 806 999 2293 2474 2683 178
Size: 94 534 193 1294 181 209 1790
Fragments arranged by size:
1790 1294 534 209 193 181 94
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 907 1672 907
Size: 765 3530
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 694 1275 694
Size: 581 3714
BstYI r'GATC_y
Cuts at: 1 644 989 1320 1787 1810 2145 2391 3403
Size: 643 345 331 467 23 335 246 1012
Cuts at: 3403 3489 3501 3847 1
Size: 86 12 346 449
Fragments arranged by size:
1012 643 467 449 346 345 335 331
246 86 23 12
BtgI C'CryG_G
Cuts at: 449 911 1274 449
Size: 462 363 3470
Fragments arranged by size:
3470 462 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 868 908 1538 868
Size: 40 630 3625
Fragments arranged by size:
3625 630 40
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 251 753 251
Size: 502 3793
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 399 1001 1880 2054 2445 2472 3817 399
Size: 602 879 174 391 27 1345 877
Fragments arranged by size:
1345 879 877 602 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 340 2318 2528 340
Size: 1978 210 2107
Fragments arranged by size:
2107 1978 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 982 2503 2813 2959 4105 982
Size: 1521 310 146 1146 1172
Fragments arranged by size:
1521 1172 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 838 2120 2552 3299 3567 497
Size: 341 1282 432 747 268 1225
Fragments arranged by size:
1282 1225 747 432 341 268
EcoNI CCTnn'n_nnAGG
Cuts at: 139 139
Size: 4295
EcoO109I rG'GnC_Cy
Cuts at: 582 1214 1434 582
Size: 632 220 3443
Fragments arranged by size:
3443 632 220
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 536 1262 1729 1977 2999 3978 536
Size: 726 467 248 1022 979 853
Fragments arranged by size:
1022 979 853 726 467 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 648 680 2137 2167 2169 2199 648
Size: 32 1457 30 2 30 2744
Fragments arranged by size:
2744 1457 32 30 30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
KasI G'GCGC_C
Cuts at: 532 1725 1973 532
Size: 1193 248 2854
Fragments arranged by size:
2854 1193 248
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 401 2056 401
Size: 1655 2640
MslI CAynn'nnrTG
Cuts at: 304 679 2284 2411 304
Size: 375 1605 127 2188
Fragments arranged by size:
2188 1605 375 127
MspA1I CmG'CkG
Cuts at: 459 1406 1720 2080 3093 3338 459
Size: 947 314 360 1013 245 1416
Fragments arranged by size:
1416 1013 947 360 314 245
NaeI GCC'GGC
Cuts at: 808 1001 2476 808
Size: 193 1475 2627
Fragments arranged by size:
2627 1475 193
NarI GG'CG_CC
Cuts at: 533 1726 1974 533
Size: 1193 248 2854
Fragments arranged by size:
2854 1193 248
NcoI C'CATG_G
Cuts at: 449 1274 449
Size: 825 3470
NgoMIV G'CCGG_C
Cuts at: 806 999 2474 806
Size: 193 1475 2627
Fragments arranged by size:
2627 1475 193
NheI G'CTAG_C
Cuts at: 1060 1060
Size: 4295
NspI r_CATG'y
Cuts at: 354 441 1053 1348 1848 2755 354
Size: 87 612 295 500 907 1894
Fragments arranged by size:
1894 907 612 500 295 87
PciI A'CATG_T
Cuts at: 437 2751 437
Size: 2314 1981
PfoI T'CCnGG_A
Cuts at: 203 474 203
Size: 271 4024
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PmlI CAC'GTG
Cuts at: 387 387
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PspOMI G'GGCC_C
Cuts at: 1214 1264 1214
Size: 50 4245
PstI C_TGCA'G
Cuts at: 863 903 1342 2027 863
Size: 40 439 685 3131
Fragments arranged by size:
3131 685 439 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 459 1720 2080 459
Size: 1261 360 2674
Fragments arranged by size:
2674 1261 360
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 1027 3812 1027
Size: 2785 1510
SfcI C'TryA_G
Cuts at: 33 859 899 1338 2023 3016 3207 33
Size: 826 40 439 685 993 191 1121
Fragments arranged by size:
1121 993 826 685 439 191 40
SfoI GGC'GCC
Cuts at: 534 1727 1975 534
Size: 1193 248 2854
Fragments arranged by size:
2854 1193 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 958 1450 2365 2857 3119 958
Size: 492 915 492 262 2134
Fragments arranged by size:
2134 915 492 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 1018 1018
Size: 4295
StyI C'CwwG_G
Cuts at: 263 449 1274 1429 1783 263
Size: 186 825 155 354 2775
Fragments arranged by size:
2775 825 354 186 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 379 1878 2011 2470 2679 379
Size: 1499 133 459 209 1995
Fragments arranged by size:
1995 1499 459 209 133
TatI w'GTAC_w
Cuts at: 1025 3810 1025
Size: 2785 1510
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 456 1790 456
Size: 1334 2961
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 470 470
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI