Restriction map for
pF1KB4176
(Circular) (Six-base) MAPSORT of: pF1KB4176.seq Check: 3629 from: 1 to: 4466
pF1KB4176 4466 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 574 1504 574
Size: 930 3536
Acc65I G'GTAC_C
Cuts at: 1396 1482 1396
Size: 86 4380
AclI AA'CG_TT
Cuts at: 408 4306 408
Size: 3898 568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 665 1343 2291 2723 3076 3738 326
Size: 339 678 948 432 353 662 1054
Fragments arranged by size:
1054 948 678 662 432 353 339
AflIII A'CryG_T
Cuts at: 1818 3618 3899 1818
Size: 1800 281 2385
Fragments arranged by size:
2385 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1365 1365
Size: 4466
AlwNI CAG_nnn'CTG
Cuts at: 144 1216 1771 3209 144
Size: 1072 555 1438 1401
Fragments arranged by size:
1438 1401 1072 555
ApaLI G'TGCA_C
Cuts at: 3304 3304
Size: 4466
ApoI r'AATT_y
Cuts at: 239 415 548 655 1011 1096 1470 3819 4075
Size: 176 133 107 356 85 374 2349 256
Cuts at: 4075 239
Size: 630
Fragments arranged by size:
2349 630 374 356 256 176 133 107
85
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 1486 1486
Size: 4466
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 1396 1482 1896 2144 2179 1396
Size: 86 414 248 35 3683
Fragments arranged by size:
3683 414 248 86 35
BanII G_rGCy'C
Cuts at: 1480 2510 1480
Size: 1030 3436
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 118 303 118
Size: 185 4281
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3420 4466 2364
Size: 1056 1046 2364
Fragments arranged by size:
2364 1056 1046
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BfrBI ATG'CAT
Cuts at: 670 712 1072 670
Size: 42 360 4064
Fragments arranged by size:
4064 360 42
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 1578 1578
Size: 4466
Bme1580I G_kGCm'C
Cuts at: 2091 2184 3308 2091
Size: 93 1124 3249
Fragments arranged by size:
3249 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 298 1425 1678 1759 2094 4238 298
Size: 1127 253 81 335 2144 526
Fragments arranged by size:
2144 1127 526 335 253 81
BmtI G_CTAG'C
Cuts at: 464 464
Size: 4466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 461
Size: 4466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2521 3235 3533 1642
Size: 879 714 298 2575
Fragments arranged by size:
2575 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4170 4170
Size: 4466
BsaAI yAC'GTr
Cuts at: 327 1819 327
Size: 1492 2974
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 1897 2145 2847 4343 1897
Size: 248 702 1496 2020
Fragments arranged by size:
2020 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3265 3412 3686 1655
Size: 521 678 411 147 274 2435
Fragments arranged by size:
2435 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 768 1891 2649 3314 3722 768
Size: 1123 758 665 408 1512
Fragments arranged by size:
1512 1123 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3284 104
Size: 1904 46 1230 1286
Fragments arranged by size:
1904 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1008 1480 2258 2448 3308 1008
Size: 472 778 190 860 2166
Fragments arranged by size:
2166 860 778 472 190
BsmI GAATG_Cn'
Cuts at: 607 607
Size: 4466
Bsp1286I G_dGCh'C
Cuts at: 1008 1480 2091 2184 2258 2448 2510 3308 1008
Size: 472 611 93 74 190 62 798 2166
Fragments arranged by size:
2166 798 611 472 190 93 74 62
BspHI T'CATG_A
Cuts at: 893 2898 893
Size: 2005 2461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 773 1516 1730 2032 2413 773
Size: 743 214 302 381 2826
Fragments arranged by size:
2826 743 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2758 2812 4296 4461 50
Size: 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2378 2378
Size: 4466
BsrFI r'CCGG_y
Cuts at: 2464 2645 2464
Size: 181 4285
BssSI C'ACGA_G
Cuts at: 2737 3445 2737
Size: 708 3758
BstAPI GCAn_nnn'nTGC
Cuts at: 144 1843 144
Size: 1699 2767
BstBI TT'CG_AA
Cuts at: 546 2827 546
Size: 2281 2185
BstYI r'GATC_y
Cuts at: 1 1248 1491 1958 1981 2316 2562 2868 2880
Size: 1247 243 467 23 335 246 306 12
Cuts at: 2880 2966 4018 1
Size: 86 1052 449
Fragments arranged by size:
1247 1052 467 449 335 306 246 243
86 23 12
BstZ17I GTA'TAC
Cuts at: 575 575
Size: 4466
BtgI C'CryG_G
Cuts at: 1043 1043
Size: 4466
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 1217 1709 1217
Size: 492 3974
DraI TTT'AAA
Cuts at: 413 420 1430 1465 413
Size: 7 1010 35 3414
Fragments arranged by size:
3414 1010 35 7
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3516 2172
Size: 1344 3122
EaeI y'GGCC_r
Cuts at: 2051 2225 2616 2643 3988 2051
Size: 174 391 27 1345 2529
Fragments arranged by size:
2529 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 1141 2489 2699 1141
Size: 1348 210 2908
Fragments arranged by size:
2908 1348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 3416 3562 4276 2674
Size: 742 146 714 2864
Fragments arranged by size:
2864 742 714 146
EcoICRI GAG'CTC
Cuts at: 1478 1478
Size: 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 461 665 1343 2291 2723 3076 3738 326
Size: 135 204 678 948 432 353 662 1054
Fragments arranged by size:
1054 948 678 662 432 353 204 135
EcoNI CCTnn'n_nnAGG
Cuts at: 1445 1445
Size: 4466
EcoO109I rG'GnC_Cy
Cuts at: 168 424 1605 168
Size: 256 1181 3029
Fragments arranged by size:
3029 1181 256
EcoRI G'AATT_C
Cuts at: 239 548 1011 1470 239
Size: 309 463 459 3235
Fragments arranged by size:
3235 463 459 309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4466
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 1900 2148 3378 4149 1900
Size: 248 1230 771 2217
Fragments arranged by size:
2217 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1383 2308 2338 2340 2370 1351
Size: 32 925 30 2 30 3447
Fragments arranged by size:
3447 925 32 30 30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1400 1486 1400
Size: 86 4380
MfeI C'AATT_G
Cuts at: 371 371
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3225 3409 3225
Size: 184 4282
MscI TGG'CCA
Cuts at: 2227 2227
Size: 4466
MslI CAynn'nnrTG
Cuts at: 325 2455 2582 325
Size: 2130 127 2209
Fragments arranged by size:
2209 2130 127
MspA1I CmG'CkG
Cuts at: 1321 1577 1891 2251 3035 3280 1321
Size: 256 314 360 784 245 2507
Fragments arranged by size:
2507 784 360 314 256 245
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NcoI C'CATG_G
Cuts at: 1043 1043
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NheI G'CTAG_C
Cuts at: 460 460
Size: 4466
NsiI A_TGCA'T
Cuts at: 672 714 1074 672
Size: 42 360 4064
Fragments arranged by size:
4064 360 42
NspI r_CATG'y
Cuts at: 138 1519 2019 3622 138
Size: 1381 500 1603 982
Fragments arranged by size:
1603 1381 982 500
PciI A'CATG_T
Cuts at: 3618 3618
Size: 4466
PflMI CCAn_nnn'nTGG
Cuts at: 1049 1049
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 4434
PpuMI rG'GwC_Cy
Cuts at: 168 424 168
Size: 256 4210
PstI C_TGCA'G
Cuts at: 1513 2198 1513
Size: 685 3781
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 1321 1891 2251 1321
Size: 570 360 3536
Fragments arranged by size:
3536 570 360
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1480 1480
Size: 4466
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SanDI GG'GwC_CC
Cuts at: 168 168
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 1509 2194 3162 3353 33
Size: 1476 685 968 191 1146
Fragments arranged by size:
1476 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 1488 1488
Size: 4466
SmlI C'TyrA_G
Cuts at: 1621 2536 3250 3512 1621
Size: 915 714 262 2575
Fragments arranged by size:
2575 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
StyI C'CwwG_G
Cuts at: 1043 1600 1954 1043
Size: 557 354 3555
Fragments arranged by size:
3555 557 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2049 2182 2641 2850 2049
Size: 133 459 209 3665
Fragments arranged by size:
3665 459 209 133
TatI w'GTAC_w
Cuts at: 723 805 3981 723
Size: 82 3176 1208
Fragments arranged by size:
3176 1208 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4434
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1961 183
Size: 1778 2688
XmaI C'CCGG_G
Cuts at: 1486 1486
Size: 4466
XmnI GAAnn'nnTTC
Cuts at: 1336 1336
Size: 4466
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NdeI NotI NruI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI