Restriction map for
pF1KB4184
(Circular) (Six-base) MAPSORT of: pF1KB4184.seq Check: 3574 from: 1 to: 4610
pF1KB4184 4610 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 511 511
Size: 4610
AccI GT'mk_AC
Cuts at: 1648 1648
Size: 4610
Acc65I G'GTAC_C
Cuts at: 1626 1626
Size: 4610
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2867 3220 3882 2435
Size: 432 353 662 3163
Fragments arranged by size:
3163 662 432 353
AflIII A'CryG_T
Cuts at: 1962 3762 4043 1962
Size: 1800 281 2529
Fragments arranged by size:
2529 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 640 640
Size: 4610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 4610
AlwNI CAG_nnn'CTG
Cuts at: 1915 3353 1915
Size: 1438 3172
ApaI G_GGCC'C
Cuts at: 924 1557 1564 924
Size: 633 7 3970
Fragments arranged by size:
3970 633 7
ApaLI G'TGCA_C
Cuts at: 3448 3448
Size: 4610
ApoI r'AATT_y
Cuts at: 247 1614 3963 4219 247
Size: 1367 2349 256 638
Fragments arranged by size:
2349 1367 638 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 709 1630 709
Size: 921 3689
AvrII C'CTAG_G
Cuts at: 660 660
Size: 4610
BamHI G'GATC_C
Cuts at: 149 1292 1635 149
Size: 1143 343 3124
Fragments arranged by size:
3124 1143 343
BanI G'GyrC_C
Cuts at: 705 893 1626 2040 2288 2323 705
Size: 188 733 414 248 35 2992
Fragments arranged by size:
2992 733 414 248 188 35
BanII G_rGCy'C
Cuts at: 578 601 659 823 924 974 1207 1557 1564
Size: 23 58 164 101 50 233 350 7
Cuts at: 1564 1624 2654 578
Size: 60 1030 2534
Fragments arranged by size:
2534 1030 350 233 164 101 60 58
50 23 7
BbeI G_GCGC'C
Cuts at: 897 2044 2292 897
Size: 1147 248 3215
Fragments arranged by size:
3215 1147 248
BbsI GAAGACnn'nnnn_
Cuts at: 504 504
Size: 4610
BbvCI CC'TCA_GC
Cuts at: 1258 1258
Size: 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4576
BciVI GTATCCnnnnn_n'
Cuts at: 347 2508 3564 4610 347
Size: 2161 1056 1046 347
Fragments arranged by size:
2161 1056 1046 347
BclI T'GATC_A
Cuts at: 485 2130 485
Size: 1645 2965
BglI GCCn_nnn'nGGC
Cuts at: 796 796
Size: 4610
BglII A'GATC_T
Cuts at: 1514 2125 4162 1514
Size: 611 2037 1962
Fragments arranged by size:
2037 1962 611
BlpI GC'TnA_GC
Cuts at: 600 1722 600
Size: 1122 3488
Bme1580I G_kGCm'C
Cuts at: 710 891 924 1557 1564 2235 2328 3452 710
Size: 181 33 633 7 671 93 1124 1868
Fragments arranged by size:
1868 1124 671 633 181 93 33 7
BmrI ACTGGGnnnn_n'
Cuts at: 1076 1822 1903 2238 4382 1076
Size: 746 81 335 2144 1304
Fragments arranged by size:
2144 1304 746 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 703 865 874 1600 299
Size: 404 162 9 726 3309
Fragments arranged by size:
3309 726 404 162 9
Bpu10I CC'TnA_GC
Cuts at: 1258 1258
Size: 4610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1786 2665 3379 3677 1786
Size: 879 714 298 2719
Fragments arranged by size:
2719 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1483 4314 1483
Size: 2831 1779
BsaAI yAC'GTr
Cuts at: 1568 1963 1568
Size: 395 4215
BsaBI GATnn'nnATC
Cuts at: 6 2124 4161 6
Size: 2118 2037 455
Fragments arranged by size:
2118 2037 455
BsaHI Gr'CG_yC
Cuts at: 894 2041 2289 2991 4487 894
Size: 1147 248 702 1496 1017
Fragments arranged by size:
1496 1147 1017 702 248
BsaWI w'CCGG_w
Cuts at: 1016 1799 2320 2998 3409 3556 3830 1016
Size: 783 521 678 411 147 274 1796
Fragments arranged by size:
1796 783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 797 827 991 1021 1399 1429 797
Size: 30 164 30 378 30 3978
Fragments arranged by size:
3978 378 164 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 596 754 862 871 874 942 996 1587 596
Size: 158 108 9 3 68 54 591 3619
Fragments arranged by size:
3619 591 158 108 68 54 9 3
BseYI C'CCAG_C
Cuts at: 390 492 1166 2035 2793 3458 3866 390
Size: 102 674 869 758 665 408 1134
Fragments arranged by size:
1134 869 758 674 665 408 102
BsiEI CG_ry'CG
Cuts at: 104 2152 2198 3428 104
Size: 2048 46 1230 1286
Fragments arranged by size:
2048 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 363 578 601 659 974 1207 1624 2402 2592
Size: 215 23 58 315 233 417 778 190
Cuts at: 2592 3452 363
Size: 860 1521
Fragments arranged by size:
1521 860 778 417 315 233 215 190
58 23
Bsp1286I G_dGCh'C
Cuts at: 363 578 601 659 710 823 891 924 974
Size: 215 23 58 51 113 68 33 50
Cuts at: 974 1207 1557 1564 1624 2235 2328 2402 2592
Size: 233 350 7 60 611 93 74 190
Cuts at: 2592 2654 3452 363
Size: 62 798 1521
Fragments arranged by size:
1521 798 611 350 233 215 190 113
93 74 68 62 60 58 51 50
33 23 7
BspEI T'CCGG_A
Cuts at: 1016 1016
Size: 4610
BspHI T'CATG_A
Cuts at: 3042 3042
Size: 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 361 511 1171 1660 1874 2176 2557 361
Size: 150 660 489 214 302 381 2414
Fragments arranged by size:
2414 660 489 381 302 214 150
BsrBI CCG'CTC
Cuts at: 50 2902 2956 4440 4605 50
Size: 2852 54 1484 165 55
Fragments arranged by size:
2852 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2522 2522
Size: 4610
BsrFI r'CCGG_y
Cuts at: 175 2608 2789 175
Size: 2433 181 1996
Fragments arranged by size:
2433 1996 181
BssHII G'CGCG_C
Cuts at: 864 864
Size: 4610
BssSI C'ACGA_G
Cuts at: 505 946 2881 3589 505
Size: 441 1935 708 1526
Fragments arranged by size:
1935 1526 708 441
BstAPI GCAn_nnn'nTGC
Cuts at: 367 1346 1987 367
Size: 979 641 2990
Fragments arranged by size:
2990 979 641
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 390 552 702 390
Size: 162 150 4298
Fragments arranged by size:
4298 162 150
BstYI r'GATC_y
Cuts at: 1 149 1043 1292 1514 1635 2102 2125 2460
Size: 148 894 249 222 121 467 23 335
Cuts at: 2460 2706 3012 3024 3110 4162 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 894 467 449 335 306 249 246
222 148 121 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 569 671 925 569
Size: 102 254 4254
Fragments arranged by size:
4254 254 102
BtgI C'CryG_G
Cuts at: 898 965 1232 898
Size: 67 267 4276
Fragments arranged by size:
4276 267 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2560 2741 2904 2560
Size: 181 163 4266
Fragments arranged by size:
4266 181 163
BtsI GCAGTG_nn'
Cuts at: 286 361 966 1853 286
Size: 75 605 887 3043
Fragments arranged by size:
3043 887 605 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 929 964 929
Size: 35 4575
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3660 2316
Size: 1344 3266
EaeI y'GGCC_r
Cuts at: 380 590 692 1229 1298 1322 2195 2369 2760
Size: 210 102 537 69 24 873 174 391
Cuts at: 2760 2787 4132 380
Size: 27 1345 858
Fragments arranged by size:
1345 873 858 537 391 210 174 102
69 27 24
EagI C'GGCC_G
Cuts at: 2195 2195
Size: 4610
EarI CTCTTCn'nnn_
Cuts at: 2633 2843 2633
Size: 210 4400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2818 3560 3706 4420 2818
Size: 742 146 714 3008
Fragments arranged by size:
3008 742 714 146
EcoICRI GAG'CTC
Cuts at: 576 599 657 972 1205 1622 576
Size: 23 58 315 233 417 3564
Fragments arranged by size:
3564 417 315 233 58 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 703 865 874 1600 2435 2867 3220 3882
Size: 404 162 9 726 835 432 353 662
Cuts at: 3882 299
Size: 1027
Fragments arranged by size:
1027 835 726 662 432 404 353 162
9
EcoO109I rG'GnC_Cy
Cuts at: 373 921 1560 1561 1749 373
Size: 548 639 1 188 3234
Fragments arranged by size:
3234 639 548 188 1
EcoRI G'AATT_C
Cuts at: 247 1614 247
Size: 1367 3243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 4610
FspI TGC'GCA
Cuts at: 2391 2391
Size: 4610
HaeII r_GCGC'y
Cuts at: 566 718 897 1357 2044 2292 3522 4293 566
Size: 152 179 460 687 248 1230 771 883
Fragments arranged by size:
1230 883 771 687 460 248 179 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 626 658 1107 1139 1399 1431 2452 2482 2484
Size: 32 449 32 260 32 1021 30 2
Cuts at: 2484 2514 626
Size: 30 2722
Fragments arranged by size:
2722 1021 449 260 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1595 1649 1595
Size: 54 4556
KasI G'GCGC_C
Cuts at: 893 2040 2288 893
Size: 1147 248 3215
Fragments arranged by size:
3215 1147 248
KpnI G_GTAC'C
Cuts at: 1630 1630
Size: 4610
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 624 3369 3553 624
Size: 2745 184 1681
Fragments arranged by size:
2745 1681 184
MscI TGG'CCA
Cuts at: 382 592 694 1231 1300 1324 2371 382
Size: 210 102 537 69 24 1047 2621
Fragments arranged by size:
2621 1047 537 210 102 69 24
MslI CAynn'nnrTG
Cuts at: 427 2599 2726 427
Size: 2172 127 2311
Fragments arranged by size:
2311 2172 127
MspA1I CmG'CkG
Cuts at: 698 1721 2035 2395 3179 3424 698
Size: 1023 314 360 784 245 1884
Fragments arranged by size:
1884 1023 784 360 314 245
NaeI GCC'GGC
Cuts at: 177 2791 177
Size: 2614 1996
NarI GG'CG_CC
Cuts at: 894 2041 2289 894
Size: 1147 248 3215
Fragments arranged by size:
3215 1147 248
NcoI C'CATG_G
Cuts at: 898 965 1232 898
Size: 67 267 4276
Fragments arranged by size:
4276 267 67
NgoMIV G'CCGG_C
Cuts at: 175 2789 175
Size: 2614 1996
NspI r_CATG'y
Cuts at: 1663 2163 3766 1663
Size: 500 1603 2507
Fragments arranged by size:
2507 1603 500
PasI CC'CwG_GG
Cuts at: 342 1557 342
Size: 1215 3395
PciI A'CATG_T
Cuts at: 3762 3762
Size: 4610
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PmlI CAC'GTG
Cuts at: 1568 1568
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 4578
PpuMI rG'GwC_Cy
Cuts at: 373 373
Size: 4610
PspOMI G'GGCC_C
Cuts at: 920 1553 1560 920
Size: 633 7 3970
Fragments arranged by size:
3970 633 7
PstI C_TGCA'G
Cuts at: 1202 1657 2342 1202
Size: 455 685 3470
Fragments arranged by size:
3470 685 455
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4610
PvuII CAG'CTG
Cuts at: 698 2035 2395 698
Size: 1337 360 2913
Fragments arranged by size:
2913 1337 360
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 578 601 659 974 1207 1624 578
Size: 23 58 315 233 417 3564
Fragments arranged by size:
3564 417 315 233 58 23
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 2633 2843 2633
Size: 210 4400
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 4127 4127
Size: 4610
SfcI C'TryA_G
Cuts at: 33 1198 1653 2338 3306 3497 33
Size: 1165 455 685 968 191 1146
Fragments arranged by size:
1165 1146 968 685 455 191
SfoI GGC'GCC
Cuts at: 895 2042 2290 895
Size: 1147 248 3215
Fragments arranged by size:
3215 1147 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 711 1632 711
Size: 921 3689
SmlI C'TyrA_G
Cuts at: 1765 2680 3394 3656 1765
Size: 915 714 262 2719
Fragments arranged by size:
2719 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1663 2163 1663
Size: 500 4110
StuI AGG'CCT
Cuts at: 350 676 790 1329 1411 350
Size: 326 114 539 82 3549
Fragments arranged by size:
3549 539 326 114 82
StyI C'CwwG_G
Cuts at: 377 660 898 965 1232 1440 1520 1744 2098
Size: 283 238 67 267 208 80 224 354
Cuts at: 2098 377
Size: 2889
Fragments arranged by size:
2889 354 283 267 238 224 208 80
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2193 2326 2785 2994 2193
Size: 133 459 209 3809
Fragments arranged by size:
3809 459 209 133
TatI w'GTAC_w
Cuts at: 4125 4125
Size: 4610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4578
Tth111I GACn'n_nGTC
Cuts at: 2407 2407
Size: 4610
XbaI T'CTAG_A
Cuts at: 130 1641 130
Size: 1511 3099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2105 2105
Size: 4610
XmaI C'CCGG_G
Cuts at: 709 1630 709
Size: 921 3689
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AloI AscI BaeI
BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI