Restriction map for
pF1KB4188
(Circular) (Six-base) MAPSORT of: pF1KB4188.seq Check: 624 from: 1 to: 4184
pF1KB4188 4184 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 451 1184 2009 2441 3188 3456 227
Size: 224 733 825 432 747 268 955
Fragments arranged by size:
955 825 747 733 432 268 224
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1112 1112
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 1489 3056 1489
Size: 1567 2617
ApaI G_GGCC'C
Cuts at: 1153 1153
Size: 4184
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 439 1130 1188 3537 3793 439
Size: 691 58 2349 256 830
Fragments arranged by size:
2349 830 691 256 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 677 1204 677
Size: 527 3657
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 1200 1614 1862 1897 1200
Size: 414 248 35 3487
Fragments arranged by size:
3487 414 248 35
BanII G_rGCy'C
Cuts at: 595 1153 1198 2228 595
Size: 558 45 1030 2551
Fragments arranged by size:
2551 1030 558 45
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 1143 1143
Size: 4184
BbvCI CC'TCA_GC
Cuts at: 557 557
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 904 2082 2843 4184 904
Size: 1178 761 1341 904
Fragments arranged by size:
1341 1178 904 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 691 691
Size: 4184
BglII A'GATC_T
Cuts at: 152 1699 3736 152
Size: 1547 2037 600
Fragments arranged by size:
2037 1547 600
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 1153 1809 1902 2958 1153
Size: 656 93 1056 2379
Fragments arranged by size:
2379 1056 656 93
BmgBI CAC'GTC
Cuts at: 624 624
Size: 4184
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 359
Size: 4184
Bpu10I CC'TnA_GC
Cuts at: 557 557
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2239 2731 3029 1360
Size: 879 492 298 2515
Fragments arranged by size:
2515 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 377 3888 377
Size: 3511 673
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 580 1698 3735 6
Size: 574 1118 2037 455
Fragments arranged by size:
2037 1118 574 455
BsaHI Gr'CG_yC
Cuts at: 1615 1863 2565 4061 1615
Size: 248 702 1496 1738
Fragments arranged by size:
1738 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1152 1609 2367 2944 3440 1152
Size: 457 758 577 496 1896
Fragments arranged by size:
1896 758 577 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 751
Size: 4184
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1198 1976 2166 2958 1198
Size: 778 190 792 2424
Fragments arranged by size:
2424 792 778 190
BsmI GAATG_Cn'
Cuts at: 342 342
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 595 1153 1198 1809 1902 1976 2166 2228 2958
Size: 558 45 611 93 74 190 62 730
Cuts at: 2958 595
Size: 1821
Fragments arranged by size:
1821 730 611 558 190 93 74 62
45
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1234 1448 1750 2131 1234
Size: 214 302 381 3287
Fragments arranged by size:
3287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 933 2476 2530 4014 4179 50
Size: 883 1543 54 1484 165 55
Fragments arranged by size:
1543 1484 883 165 55 54
BsrDI GCAATG_nn'
Cuts at: 362 2096 362
Size: 1734 2450
BsrFI r'CCGG_y
Cuts at: 121 2182 2363 2572 121
Size: 2061 181 209 1733
Fragments arranged by size:
2061 1733 209 181
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 1134 2545 1134
Size: 1411 2773
BstYI r'GATC_y
Cuts at: 1 152 1209 1676 1699 2034 2280 3292 3378
Size: 151 1057 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1057 1012 467 449 346 335 246 151
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 450 770 1769 1943 2334 2361 3706 450
Size: 320 999 174 391 27 1345 928
Fragments arranged by size:
1345 999 928 391 320 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2392 2702 2848 3994 2392
Size: 310 146 1146 2582
Fragments arranged by size:
2582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 359 451 1184 2009 2441 3188 3456 227
Size: 132 92 733 825 432 747 268 955
Fragments arranged by size:
955 825 747 733 432 268 132 92
EcoNI CCTnn'n_nnAGG
Cuts at: 289 289
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 1111 1149 1323 1111
Size: 38 174 3972
Fragments arranged by size:
3972 174 38
EcoRI G'AATT_C
Cuts at: 1130 1188 1130
Size: 58 4126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 1618 1866 2888 3867 1618
Size: 248 1022 979 1935
Fragments arranged by size:
1935 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2026 2056 2058 2088 2026
Size: 30 2 30 4122
Fragments arranged by size:
4122 30 30 2
HincII GTy'rAC
Cuts at: 1037 1223 1037
Size: 186 3998
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 269 716 2855 3039 269
Size: 447 2139 184 1414
Fragments arranged by size:
2139 1414 447 184
MscI TGG'CCA
Cuts at: 452 772 1945 452
Size: 320 1173 2691
Fragments arranged by size:
2691 1173 320
MslI CAynn'nnrTG
Cuts at: 386 860 2173 2300 386
Size: 474 1313 127 2270
Fragments arranged by size:
2270 1313 474 127
MspA1I CmG'CkG
Cuts at: 556 1156 1295 1609 1969 2982 3227 556
Size: 600 139 314 360 1013 245 1513
Fragments arranged by size:
1513 1013 600 360 314 245 139
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 920 1081 1237 1737 2644 920
Size: 161 156 500 907 2460
Fragments arranged by size:
2460 907 500 161 156
PasI CC'CwG_GG
Cuts at: 342 342
Size: 4184
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 1111 1111
Size: 4184
PsiI TTA'TAA
Cuts at: 763 763
Size: 4184
PspOMI G'GGCC_C
Cuts at: 1149 1149
Size: 4184
PstI C_TGCA'G
Cuts at: 555 1044 1119 1231 1916 555
Size: 489 75 112 685 2823
Fragments arranged by size:
2823 685 489 112 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 556 1609 1969 556
Size: 1053 360 2771
Fragments arranged by size:
2771 1053 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 1119 1231 1119
Size: 112 4072
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SfcI C'TryA_G
Cuts at: 33 551 1040 1115 1227 1912 2905 3096 33
Size: 518 489 75 112 685 993 191 1121
Fragments arranged by size:
1121 993 685 518 489 191 112 75
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 677 1339 2254 2746 3008 677
Size: 662 915 492 262 1853
Fragments arranged by size:
1853 915 662 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1081 1237 1737 1081
Size: 156 500 3528
Fragments arranged by size:
3528 500 156
StyI C'CwwG_G
Cuts at: 818 1318 1672 818
Size: 500 354 3330
Fragments arranged by size:
3330 500 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 616 1767 1900 2359 2568 616
Size: 1151 133 459 209 2232
Fragments arranged by size:
2232 1151 459 209 133
TatI w'GTAC_w
Cuts at: 179 1062 3699 179
Size: 883 2637 664
Fragments arranged by size:
2637 883 664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4152
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1679 1679
Size: 4184
XhoI C'TCGA_G
Cuts at: 677 677
Size: 4184
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
XmnI GAAnn'nnTTC
Cuts at: 686 686
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI