Restriction map for
pF1KB4192
(Circular) (Six-base) MAPSORT of: pF1KB4192.seq Check: 6171 from: 1 to: 4592
pF1KB4192 4592 bp
With 182 enzymes: SgfI *
January 17, 2012 13:59 ..
AatII G_ACGT'C
Cuts at: 797 797
Size: 4592
AccI GT'mk_AC
Cuts at: 497 653 1630 497
Size: 156 977 3459
Fragments arranged by size:
3459 977 156
Acc65I G'GTAC_C
Cuts at: 1608 1608
Size: 4592
AclI AA'CG_TT
Cuts at: 4432 4432
Size: 4592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 1488 2417 2849 3596 3864 395
Size: 1093 929 432 747 268 1123
Fragments arranged by size:
1123 1093 929 747 432 268
AflIII A'CryG_T
Cuts at: 1088 1944 3048 4025 1088
Size: 856 1104 977 1655
Fragments arranged by size:
1655 1104 977 856
AgeI A'CCGG_T
Cuts at: 2980 2980
Size: 4592
AleI CACnn'nnGTG
Cuts at: 761 761
Size: 4592
AlwNI CAG_nnn'CTG
Cuts at: 548 1344 1897 3464 548
Size: 796 553 1567 1676
Fragments arranged by size:
1676 1567 796 553
ApaLI G'TGCA_C
Cuts at: 3362 3362
Size: 4592
ApoI r'AATT_y
Cuts at: 1596 3945 4201 1596
Size: 2349 256 1987
Fragments arranged by size:
2349 1987 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4592
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4592
AvaI C'yCGr_G
Cuts at: 738 870 1186 1196 1360 1531 1612 738
Size: 132 316 10 164 171 81 3718
Fragments arranged by size:
3718 316 171 164 132 81 10
BamHI G'GATC_C
Cuts at: 1617 1617
Size: 4592
BanI G'GyrC_C
Cuts at: 766 1304 1348 1608 2022 2270 2305 766
Size: 538 44 260 414 248 35 3053
Fragments arranged by size:
3053 538 414 260 248 44 35
BanII G_rGCy'C
Cuts at: 120 1174 1286 1516 1606 2636 120
Size: 1054 112 230 90 1030 2076
Fragments arranged by size:
2076 1054 1030 230 112 90
BbeI G_GCGC'C
Cuts at: 770 1352 2026 2274 770
Size: 582 674 248 3088
Fragments arranged by size:
3088 674 582 248
BbsI GAAGACnn'nnnn_
Cuts at: 954 954
Size: 4592
BciVI GTATCCnnnnn_n'
Cuts at: 240 1588 2490 3251 4592 240
Size: 1348 902 761 1341 240
Fragments arranged by size:
1348 1341 902 761 240
BclI T'GATC_A
Cuts at: 2112 2112
Size: 4592
BglI GCCn_nnn'nGGC
Cuts at: 1169 1198 1215 1169
Size: 29 17 4546
Fragments arranged by size:
4546 29 17
BglII A'GATC_T
Cuts at: 2107 4144 2107
Size: 2037 2555
BlpI GC'TnA_GC
Cuts at: 1704 1704
Size: 4592
Bme1580I G_kGCm'C
Cuts at: 857 2217 2310 3366 857
Size: 1360 93 1056 2083
Fragments arranged by size:
2083 1360 1056 93
BmgBI CAC'GTC
Cuts at: 518 518
Size: 4592
BmrI ACTGGGnnnn_n'
Cuts at: 1455 1804 1885 2220 4364 1455
Size: 349 81 335 2144 1683
Fragments arranged by size:
2144 1683 349 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 525 2959 525
Size: 2434 2158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 1500 1168
Size: 332 4260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1768 2647 3139 3437 250
Size: 1518 879 492 298 1405
Fragments arranged by size:
1518 1405 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 936 4296 936
Size: 3360 1232
BsaAI yAC'GTr
Cuts at: 1945 1945
Size: 4592
BsaBI GATnn'nnATC
Cuts at: 6 337 685 2106 4143 6
Size: 331 348 1421 2037 455
Fragments arranged by size:
2037 1421 455 348 331
BsaHI Gr'CG_yC
Cuts at: 482 767 794 1102 1222 1349 2023 2271 2973
Size: 285 27 308 120 127 674 248 702
Cuts at: 2973 4469 482
Size: 1496 605
Fragments arranged by size:
1496 702 674 605 308 285 248 127
120 27
BsaWI w'CCGG_w
Cuts at: 1781 2302 2980 3254 3401 3812 1781
Size: 521 678 274 147 411 2561
Fragments arranged by size:
2561 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 322 1267 127
Size: 195 945 3452
Fragments arranged by size:
3452 945 195
BseYI C'CCAG_C
Cuts at: 1094 2017 2775 3352 3848 1094
Size: 923 758 577 496 1838
Fragments arranged by size:
1838 923 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2134 2180 3388 104
Size: 2030 46 1208 1308
Fragments arranged by size:
2030 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 494 866 1085 1286 1408 1502 1606 2384 2574
Size: 372 219 201 122 94 104 778 190
Cuts at: 2574 3366 494
Size: 792 1720
Fragments arranged by size:
1720 792 778 372 219 201 190 122
104 94
BsmI GAATG_Cn'
Cuts at: 1467 1467
Size: 4592
Bsp1286I G_dGCh'C
Cuts at: 120 494 857 866 1085 1174 1286 1408 1502
Size: 374 363 9 219 89 112 122 94
Cuts at: 1502 1516 1606 2217 2310 2384 2574 2636 3366
Size: 14 90 611 93 74 190 62 730
Cuts at: 3366 120
Size: 1346
Fragments arranged by size:
1346 730 611 374 363 219 190 122
112 94 93 90 89 74 62 14
9
BspEI T'CCGG_A
Cuts at: 3812 3812
Size: 4592
BspHI T'CATG_A
Cuts at: 1064 3768 1064
Size: 2704 1888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1013 1642 1856 2158 2539 1013
Size: 629 214 302 381 3066
Fragments arranged by size:
3066 629 381 302 214
BsrBI CCG'CTC
Cuts at: 50 608 828 1014 1492 2884 2938 4422 4587
Size: 558 220 186 478 1392 54 1484 165
Cuts at: 4587 50
Size: 55
Fragments arranged by size:
1484 1392 558 478 220 186 165 55
54
BsrDI GCAATG_nn'
Cuts at: 974 2504 974
Size: 1530 3062
BsrFI r'CCGG_y
Cuts at: 224 900 910 1307 1426 2590 2771 2980 224
Size: 676 10 397 119 1164 181 209 1836
Fragments arranged by size:
1836 1164 676 397 209 181 119 10
BssSI C'ACGA_G
Cuts at: 418 1285 1407 2863 3221 418
Size: 867 122 1456 358 1789
Fragments arranged by size:
1789 1456 867 358 122
BstAPI GCAn_nnn'nTGC
Cuts at: 548 1969 548
Size: 1421 3171
BstBI TT'CG_AA
Cuts at: 2953 2953
Size: 4592
BstEII G'GTnAC_C
Cuts at: 270 561 270
Size: 291 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 763 763
Size: 4592
BstYI r'GATC_y
Cuts at: 1 1617 2084 2107 2442 2688 3700 3786 3798
Size: 1616 467 23 335 246 1012 86 12
Cuts at: 3798 4144 1
Size: 346 449
Fragments arranged by size:
1616 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 242 242
Size: 4592
BtgI C'CryG_G
Cuts at: 107 759 1126 1273 107
Size: 652 367 147 3426
Fragments arranged by size:
3426 652 367 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2542 2723 2886 2542
Size: 181 163 4248
Fragments arranged by size:
4248 181 163
BtsI GCAGTG_nn'
Cuts at: 560 1835 560
Size: 1275 3317
ClaI AT'CG_AT
Cuts at: 341 341
Size: 4592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1041 1076 1041
Size: 35 4557
DraI TTT'AAA
Cuts at: 1591 1591
Size: 4592
DrdI GACnn_nn'nnGTC
Cuts at: 2298 3156 2298
Size: 858 3734
EaeI y'GGCC_r
Cuts at: 226 459 530 915 2177 2351 2742 2769 4114
Size: 233 71 385 1262 174 391 27 1345
Cuts at: 4114 226
Size: 704
Fragments arranged by size:
1345 1262 704 391 385 233 174 71
27
EagI C'GGCC_G
Cuts at: 2177 2177
Size: 4592
EarI CTCTTCn'nnn_
Cuts at: 205 623 835 1507 2615 2825 205
Size: 418 212 672 1108 210 1972
Fragments arranged by size:
1972 1108 672 418 212 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 805 2800 3110 3256 4402 739
Size: 66 1995 310 146 1146 929
Fragments arranged by size:
1995 1146 929 310 146 66
EcoICRI GAG'CTC
Cuts at: 1284 1604 1284
Size: 320 4272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 1168 1488 1500 2417 2849 3596 3864 395
Size: 773 320 12 917 432 747 268 1123
Fragments arranged by size:
1123 917 773 747 432 320 268 12
EcoO109I rG'GnC_Cy
Cuts at: 129 1191 1316 1535 1731 129
Size: 1062 125 219 196 2990
Fragments arranged by size:
2990 1062 219 196 125
EcoRI G'AATT_C
Cuts at: 1596 1596
Size: 4592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 846 2611 396
Size: 450 1765 2377
Fragments arranged by size:
2377 1765 450
FspI TGC'GCA
Cuts at: 552 1453 2373 552
Size: 901 920 2771
Fragments arranged by size:
2771 920 901
FspAI rTGC'GCAy
Cuts at: 1453 1453
Size: 4592
HaeII r_GCGC'y
Cuts at: 770 1352 2026 2274 3296 4275 770
Size: 582 674 248 1022 979 1087
Fragments arranged by size:
1087 1022 979 674 582 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1423 1455 2434 2464 2466 2496 1423
Size: 32 979 30 2 30 3519
Fragments arranged by size:
3519 979 32 30 30 2
HincII GTy'rAC
Cuts at: 1631 1631
Size: 4592
KasI G'GCGC_C
Cuts at: 766 1348 2022 2270 766
Size: 582 674 248 3088
Fragments arranged by size:
3088 674 582 248
KpnI G_GTAC'C
Cuts at: 1612 1612
Size: 4592
MluI A'CGCG_T
Cuts at: 4025 4025
Size: 4592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1466 3263 3447 1466
Size: 1797 184 2611
Fragments arranged by size:
2611 1797 184
MscI TGG'CCA
Cuts at: 461 2353 461
Size: 1892 2700
MslI CAynn'nnrTG
Cuts at: 761 850 1063 2581 2708 761
Size: 89 213 1518 127 2645
Fragments arranged by size:
2645 1518 213 127 89
MspA1I CmG'CkG
Cuts at: 1098 1128 1370 1703 2017 2377 3390 3635 1098
Size: 30 242 333 314 360 1013 245 2055
Fragments arranged by size:
2055 1013 360 333 314 245 242 30
NaeI GCC'GGC
Cuts at: 226 902 912 2773 226
Size: 676 10 1861 2045
Fragments arranged by size:
2045 1861 676 10
NarI GG'CG_CC
Cuts at: 767 1349 2023 2271 767
Size: 582 674 248 3088
Fragments arranged by size:
3088 674 582 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4592
NgoMIV G'CCGG_C
Cuts at: 224 900 910 2771 224
Size: 676 10 1861 2045
Fragments arranged by size:
2045 1861 676 10
NspI r_CATG'y
Cuts at: 1645 2145 3052 1645
Size: 500 907 3185
Fragments arranged by size:
3185 907 500
PasI CC'CwG_GG
Cuts at: 781 781
Size: 4592
PciI A'CATG_T
Cuts at: 3048 3048
Size: 4592
PflMI CCAn_nnn'nTGG
Cuts at: 1344 1344
Size: 4592
PfoI T'CCnGG_A
Cuts at: 1145 1145
Size: 4592
PmeI GTTT'AAAC
Cuts at: 1591 1591
Size: 4592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 622 654 3731 3763 622
Size: 32 3077 32 1451
Fragments arranged by size:
3077 1451 32 32
PpuMI rG'GwC_Cy
Cuts at: 129 1316 129
Size: 1187 3405
PshAI GACnn'nnGTC
Cuts at: 793 793
Size: 4592
PstI C_TGCA'G
Cuts at: 1639 2324 1639
Size: 685 3907
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4592
PvuII CAG'CTG
Cuts at: 2017 2377 2017
Size: 360 4232
RsrII CG'GwC_CG
Cuts at: 2787 2787
Size: 4592
SacI G_AGCT'C
Cuts at: 1286 1606 1286
Size: 320 4272
SacII CC_GC'GG
Cuts at: 1129 1129
Size: 4592
SalI G'TCGA_C
Cuts at: 1629 1629
Size: 4592
SapI GCTCTTCn'nnn_
Cuts at: 835 1507 2615 2825 835
Size: 672 1108 210 2602
Fragments arranged by size:
2602 1108 672 210
SbfI CC_TGCA'GG
Cuts at: 1639 1639
Size: 4592
ScaI AGT'ACT
Cuts at: 4109 4109
Size: 4592
SfcI C'TryA_G
Cuts at: 33 366 1635 2320 3313 3504 33
Size: 333 1269 685 993 191 1121
Fragments arranged by size:
1269 1121 993 685 333 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1198 1198
Size: 4592
SfoI GGC'GCC
Cuts at: 768 1350 2024 2272 768
Size: 582 674 248 3088
Fragments arranged by size:
3088 674 582 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4592
SgrAI Cr'CCGG_yG
Cuts at: 1426 1426
Size: 4592
SmaI CCC'GGG
Cuts at: 740 1198 1614 740
Size: 458 416 3718
Fragments arranged by size:
3718 458 416
SmlI C'TyrA_G
Cuts at: 265 1747 2662 3154 3416 265
Size: 1482 915 492 262 1441
Fragments arranged by size:
1482 1441 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4592
SphI G_CATG'C
Cuts at: 1645 2145 1645
Size: 500 4092
SspI AAT'ATT
Cuts at: 453 453
Size: 4592
StuI AGG'CCT
Cuts at: 700 700
Size: 4592
StyI C'CwwG_G
Cuts at: 107 1213 1726 2080 107
Size: 1106 513 354 2619
Fragments arranged by size:
2619 1106 513 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1126 1437 2175 2308 2767 2976 1126
Size: 311 738 133 459 209 2742
Fragments arranged by size:
2742 738 459 311 209 133
TatI w'GTAC_w
Cuts at: 4107 4107
Size: 4592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1105 1137 4109 4141 1105
Size: 32 2972 32 1556
Fragments arranged by size:
2972 1556 32 32
Tth111I GACn'n_nGTC
Cuts at: 2389 2389
Size: 4592
XbaI T'CTAG_A
Cuts at: 1623 1623
Size: 4592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2087 2087
Size: 4592
XmaI C'CCGG_G
Cuts at: 738 1196 1612 738
Size: 458 416 3718
Fragments arranged by size:
3718 458 416
ZraI GAC'GTC
Cuts at: 795 795
Size: 4592
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SanDI SexAI SnaBI SrfI SwaI XhoI XmnI