Restriction map for
pF1KB4203
(Circular) (Six-base) MAPSORT of: pF1KB4203.seq Check: 1316 from: 1 to: 4067
pF1KB4203 4067 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 849 849
Size: 4067
AatII G_ACGT'C
Cuts at: 773 773
Size: 4067
AccI GT'mk_AC
Cuts at: 374 1105 374
Size: 731 3336
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1892 2324 3071 3339 874
Size: 1018 432 747 268 1602
Fragments arranged by size:
1602 1018 747 432 268
AflIII A'CryG_T
Cuts at: 425 1419 2523 3500 425
Size: 994 1104 977 992
Fragments arranged by size:
1104 994 992 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 4035
AlwNI CAG_nnn'CTG
Cuts at: 852 936 1372 2939 852
Size: 84 436 1567 1980
Fragments arranged by size:
1980 1567 436 84
ApaI G_GGCC'C
Cuts at: 549 549
Size: 4067
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 1071 3420 3676 1071
Size: 2349 256 1462
Fragments arranged by size:
2349 1462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 907 1087 907
Size: 180 3887
BamHI G'GATC_C
Cuts at: 302 1092 302
Size: 790 3277
BanI G'GyrC_C
Cuts at: 280 1083 1497 1745 1780 280
Size: 803 414 248 35 2567
Fragments arranged by size:
2567 803 414 248 35
BanII G_rGCy'C
Cuts at: 446 549 1081 2111 446
Size: 103 532 1030 2402
Fragments arranged by size:
2402 1030 532 103
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BbsI GAAGACnn'nnnn_
Cuts at: 1021 1061 1021
Size: 40 4027
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 408 549 1692 1785 2841 408
Size: 141 1143 93 1056 1634
Fragments arranged by size:
1634 1143 1056 141 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 4067
BmrI ACTGGGnnnn_n'
Cuts at: 238 1279 1360 1695 3839 238
Size: 1041 81 335 2144 466
Fragments arranged by size:
2144 1041 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 795 991 571
Size: 224 196 3647
Fragments arranged by size:
3647 224 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2122 2614 2912 1243
Size: 879 492 298 2398
Fragments arranged by size:
2398 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 805 1581 3618 6
Size: 799 776 2037 455
Fragments arranged by size:
2037 799 776 455
BsaHI Gr'CG_yC
Cuts at: 770 1498 1746 2448 3944 770
Size: 728 248 702 1496 893
Fragments arranged by size:
1496 893 728 702 248
BsaWI w'CCGG_w
Cuts at: 473 1256 1777 2455 2729 2876 3287 473
Size: 783 521 678 274 147 411 1253
Fragments arranged by size:
1253 783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 859 886 889 916 139
Size: 30 690 27 3 27 3290
Fragments arranged by size:
3290 690 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 883 883
Size: 4067
BseYI C'CCAG_C
Cuts at: 881 888 1492 2250 2827 3323 881
Size: 7 604 758 577 496 1625
Fragments arranged by size:
1625 758 604 577 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 4067
BsiEI CG_ry'CG
Cuts at: 104 374 897 1609 1655 2863 104
Size: 270 523 712 46 1208 1308
Fragments arranged by size:
1308 1208 712 523 270 46
BsiHKAI G_wGCw'C
Cuts at: 446 986 1081 1859 2049 2841 446
Size: 540 95 778 190 792 1672
Fragments arranged by size:
1672 792 778 540 190 95
BsmBI CGTCTCn'nnnn_
Cuts at: 416 416
Size: 4067
Bsp1286I G_dGCh'C
Cuts at: 408 446 549 986 1081 1692 1785 1859 2049
Size: 38 103 437 95 611 93 74 190
Cuts at: 2049 2111 2841 408
Size: 62 730 1634
Fragments arranged by size:
1634 730 611 437 190 103 95 93
74 62 38
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 849 1117 1331 1633 2014 849
Size: 268 214 302 381 2902
Fragments arranged by size:
2902 381 302 268 214
BsrBI CCG'CTC
Cuts at: 50 278 2359 2413 3897 4062 50
Size: 228 2081 54 1484 165 55
Fragments arranged by size:
2081 1484 228 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1022 1979 1022
Size: 957 3110
BsrFI r'CCGG_y
Cuts at: 694 2065 2246 2455 694
Size: 1371 181 209 2306
Fragments arranged by size:
2306 1371 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstEII G'GTnAC_C
Cuts at: 213 213
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 687 687
Size: 4067
BstYI r'GATC_y
Cuts at: 1 302 431 1043 1092 1559 1582 1917 2163
Size: 301 129 612 49 467 23 335 246
Cuts at: 2163 3175 3261 3273 3619 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 612 467 449 346 335 301 246
129 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 937 937
Size: 4067
BtgI C'CryG_G
Cuts at: 234 234
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 923 1310 923
Size: 387 3680
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 290 677 803 1652 1826 2217 2244 3589 290
Size: 387 126 849 174 391 27 1345 768
Fragments arranged by size:
1345 849 768 391 387 174 126 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 444 1079 444
Size: 635 3432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 795 874 991 1892 2324 3071 3339 571
Size: 224 79 117 901 432 747 268 1299
Fragments arranged by size:
1299 901 747 432 268 224 117 79
EcoO109I rG'GnC_Cy
Cuts at: 546 836 1206 546
Size: 290 370 3407
Fragments arranged by size:
3407 370 290
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 367 1848 367
Size: 1481 2586
FspAI rTGC'GCAy
Cuts at: 367 367
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 859 886 891 918 1909 1939 1941 1971 859
Size: 27 5 27 991 30 2 30 2955
Fragments arranged by size:
2955 991 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 375 1106 375
Size: 731 3336
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 2738 2922 791
Size: 1947 184 1936
Fragments arranged by size:
1947 1936 184
MscI TGG'CCA
Cuts at: 679 805 1828 679
Size: 126 1023 2918
Fragments arranged by size:
2918 1023 126
MslI CAynn'nnrTG
Cuts at: 286 292 361 587 790 2056 2183 286
Size: 6 69 226 203 1266 127 2170
Fragments arranged by size:
2170 1266 226 203 127 69 6
MspA1I CmG'CkG
Cuts at: 852 1178 1492 1852 2865 3110 852
Size: 326 314 360 1013 245 1809
Fragments arranged by size:
1809 1013 360 326 314 245
NaeI GCC'GGC
Cuts at: 696 2248 696
Size: 1552 2515
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NgoMIV G'CCGG_C
Cuts at: 694 2246 694
Size: 1552 2515
NspI r_CATG'y
Cuts at: 366 1120 1620 2527 366
Size: 754 500 907 1906
Fragments arranged by size:
1906 907 754 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PfoI T'CCnGG_A
Cuts at: 157 157
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 139 171 3206 3238 139
Size: 32 3035 32 968
Fragments arranged by size:
3035 968 32 32
PpuMI rG'GwC_Cy
Cuts at: 836 836
Size: 4067
PspOMI G'GGCC_C
Cuts at: 545 545
Size: 4067
PstI C_TGCA'G
Cuts at: 1114 1799 1114
Size: 685 3382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 852 1492 1852 852
Size: 640 360 3067
Fragments arranged by size:
3067 640 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 446 1081 446
Size: 635 3432
SalI G'TCGA_C
Cuts at: 373 1104 373
Size: 731 3336
SanDI GG'GwC_CC
Cuts at: 836 836
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 1110 1795 2788 2979 33
Size: 1077 685 993 191 1121
Fragments arranged by size:
1121 1077 993 685 191
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 1222 2137 2629 2891 1222
Size: 915 492 262 2398
Fragments arranged by size:
2398 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 458 516 1201 1555 458
Size: 58 685 354 2970
Fragments arranged by size:
2970 685 354 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 1650 1783 2242 2451 141
Size: 1509 133 459 209 1757
Fragments arranged by size:
1757 1509 459 209 133
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 225 798 1562 225
Size: 573 764 2730
Fragments arranged by size:
2730 764 573
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
ZraI GAC'GTC
Cuts at: 771 771
Size: 4067
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI