Restriction map for
pF1KB4216
(Circular) (Six-base) MAPSORT of: pF1KB4216.seq Check: 1282 from: 1 to: 6536
pF1KB4216 6536 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 3386 702
Size: 2684 3852
AatII G_ACGT'C
Cuts at: 2743 2743
Size: 6536
AccI GT'mk_AC
Cuts at: 1538 3574 1538
Size: 2036 4500
Acc65I G'GTAC_C
Cuts at: 1053 3552 1053
Size: 2499 4037
AclI AA'CG_TT
Cuts at: 6376 6376
Size: 6536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 484 634 901 1309 1384 4361 4793 5146
Size: 134 150 267 408 75 2977 432 353
Cuts at: 5146 5808 350
Size: 662 1078
Fragments arranged by size:
2977 1078 662 432 408 353 267 150
134 75
AfeI AGC'GCT
Cuts at: 3062 3062
Size: 6536
AflIII A'CryG_T
Cuts at: 3113 3888 5688 5969 3113
Size: 775 1800 281 3680
Fragments arranged by size:
3680 1800 775 281
AhdI GACnn_n'nnGTC
Cuts at: 1422 1422
Size: 6536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 791 815 2542 2902 791
Size: 24 1727 360 4425
Fragments arranged by size:
4425 1727 360 24
AlwNI CAG_nnn'CTG
Cuts at: 802 1414 1657 1690 1885 2059 2343 3124 3267
Size: 612 243 33 195 174 284 781 143
Cuts at: 3267 3841 5279 802
Size: 574 1438 2059
Fragments arranged by size:
2059 1438 781 612 574 284 243 195
174 143 33
ApaI G_GGCC'C
Cuts at: 1626 1626
Size: 6536
ApaLI G'TGCA_C
Cuts at: 5374 5374
Size: 6536
ApoI r'AATT_y
Cuts at: 2605 3540 5889 6145 2605
Size: 935 2349 256 2996
Fragments arranged by size:
2996 2349 935 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6536
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6536
AvaI C'yCGr_G
Cuts at: 2096 2353 3402 3556 2096
Size: 257 1049 154 5076
Fragments arranged by size:
5076 1049 257 154
AvrII C'CTAG_G
Cuts at: 1332 1332
Size: 6536
BamHI G'GATC_C
Cuts at: 2161 3561 2161
Size: 1400 5136
BanI G'GyrC_C
Cuts at: 1053 1835 3552 3966 4214 4249 1053
Size: 782 1717 414 248 35 3340
Fragments arranged by size:
3340 1717 782 414 248 35
BanII G_rGCy'C
Cuts at: 1082 1626 2279 2337 2674 2692 2851 3550 4580
Size: 544 653 58 337 18 159 699 1030
Cuts at: 4580 1082
Size: 3038
Fragments arranged by size:
3038 1030 699 653 544 337 159 58
18
BbeI G_GCGC'C
Cuts at: 3970 4218 3970
Size: 248 6288
BbsI GAAGACnn'nnnn_
Cuts at: 889 2022 2081 889
Size: 1133 59 5344
Fragments arranged by size:
5344 1133 59
BbvCI CC'TCA_GC
Cuts at: 2326 2326
Size: 6536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2344 2378 2344
Size: 34 6502
BciVI GTATCCnnnnn_n'
Cuts at: 4434 5490 6536 4434
Size: 1056 1046 4434
Fragments arranged by size:
4434 1056 1046
BclI T'GATC_A
Cuts at: 560 4056 560
Size: 3496 3040
BfrBI ATG'CAT
Cuts at: 2889 2889
Size: 6536
BglI GCCn_nnn'nGGC
Cuts at: 2062 2348 3091 2062
Size: 286 743 5507
Fragments arranged by size:
5507 743 286
BglII A'GATC_T
Cuts at: 1851 4051 6088 1851
Size: 2200 2037 2299
Fragments arranged by size:
2299 2200 2037
BlpI GC'TnA_GC
Cuts at: 3648 3648
Size: 6536
Bme1580I G_kGCm'C
Cuts at: 1043 1626 1929 2472 4161 4254 5378 1043
Size: 583 303 543 1689 93 1124 2201
Fragments arranged by size:
2201 1689 1124 583 543 303 93
BmrI ACTGGGnnnn_n'
Cuts at: 3748 3829 4164 6308 3748
Size: 81 335 2144 3976
Fragments arranged by size:
3976 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1527 4903 1527
Size: 3376 3160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1851 2551 2595 2600 2641 2667 2755 3466
Size: 1694 700 44 5 41 26 88 711
Cuts at: 3466 157
Size: 3227
Fragments arranged by size:
3227 1694 711 700 88 44 41 26
5
Bpu10I CC'TnA_GC
Cuts at: 1756 2326 1756
Size: 570 5966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1096 2005 2263 2683 3712 4591 5305 5603
Size: 455 909 258 420 1029 879 714 298
Cuts at: 5603 641
Size: 1574
Fragments arranged by size:
1574 1029 909 879 714 455 420 298
258
BsaI GGTCTCn'nnnn_
Cuts at: 133 142 2576 3097 6240 133
Size: 9 2434 521 3143 429
Fragments arranged by size:
3143 2434 521 429 9
BsaAI yAC'GTr
Cuts at: 3015 3889 3015
Size: 874 5662
BsaBI GATnn'nnATC
Cuts at: 6 1856 4050 6087 6
Size: 1850 2194 2037 455
Fragments arranged by size:
2194 2037 1850 455
BsaHI Gr'CG_yC
Cuts at: 1190 2740 3967 4215 4917 6413 1190
Size: 1550 1227 248 702 1496 1313
Fragments arranged by size:
1550 1496 1313 1227 702 248
BsaWI w'CCGG_w
Cuts at: 1838 3725 4246 4924 5335 5482 5756 1838
Size: 1887 521 678 411 147 274 2618
Fragments arranged by size:
2618 1887 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1798 1828 3451 3481 1798
Size: 30 1623 30 4853
Fragments arranged by size:
4853 1623 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1128 1505 1806 1883 2236 3387 1128
Size: 377 301 77 353 1151 4277
Fragments arranged by size:
4277 1151 377 353 301 77
BseYI C'CCAG_C
Cuts at: 126 162 512 518 731 1407 1527 2301 2481
Size: 36 350 6 213 676 120 774 180
Cuts at: 2481 3186 3501 3961 4719 5384 5792 126
Size: 705 315 460 758 665 408 870
Fragments arranged by size:
870 774 758 705 676 665 460 408
350 315 213 180 120 36 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 811 835 2110 2119 2237 736
Size: 75 24 1275 9 118 5035
Fragments arranged by size:
5035 1275 118 75 24 9
BsiEI CG_ry'CG
Cuts at: 104 3462 4078 4124 5354 104
Size: 3358 616 46 1230 1286
Fragments arranged by size:
3358 1286 1230 616 46
BsiHKAI G_wGCw'C
Cuts at: 2242 2360 2619 2692 2699 3304 3550 4328 4518
Size: 118 259 73 7 605 246 778 190
Cuts at: 4518 5378 2242
Size: 860 3400
Fragments arranged by size:
3400 860 778 605 259 246 190 118
73 7
BsmI GAATG_Cn'
Cuts at: 630 630
Size: 6536
BsmBI CGTCTCn'nnnn_
Cuts at: 2771 2771
Size: 6536
Bsp1286I G_dGCh'C
Cuts at: 1043 1082 1626 1929 2242 2279 2337 2360 2472
Size: 39 544 303 313 37 58 23 112
Cuts at: 2472 2619 2674 2692 2699 2851 3304 3550 4161
Size: 147 55 18 7 152 453 246 611
Cuts at: 4161 4254 4328 4518 4580 5378 1043
Size: 93 74 190 62 798 2201
Fragments arranged by size:
2201 798 611 544 453 313 303 246
190 152 147 112 93 74 62 58
55 39 37 23 18 7
BspHI T'CATG_A
Cuts at: 4968 4968
Size: 6536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 810 1041 1242 1912 2490 2534 3047 3386
Size: 108 231 201 670 578 44 513 339
Cuts at: 3386 3586 3800 4102 4483 702
Size: 200 214 302 381 2755
Fragments arranged by size:
2755 670 578 513 381 339 302 231
214 201 200 108 44
BsrBI CCG'CTC
Cuts at: 50 1544 2424 3345 4828 4882 6366 6531 50
Size: 1494 880 921 1483 54 1484 165 55
Fragments arranged by size:
1494 1484 1483 921 880 165 55 54
BsrDI GCAATG_nn'
Cuts at: 336 2492 2937 3518 4448 336
Size: 2156 445 581 930 2424
Fragments arranged by size:
2424 2156 930 581 445
BsrFI r'CCGG_y
Cuts at: 693 1888 2039 2120 3461 4534 4715 693
Size: 1195 151 81 1341 1073 181 2514
Fragments arranged by size:
2514 1341 1195 1073 181 151 81
BsrGI T'GTAC_A
Cuts at: 2178 2178
Size: 6536
BssSI C'ACGA_G
Cuts at: 4807 5515 4807
Size: 708 5828
BstAPI GCAn_nnn'nTGC
Cuts at: 714 1885 2133 3479 3913 714
Size: 1171 248 1346 434 3337
Fragments arranged by size:
3337 1346 1171 434 248
BstBI TT'CG_AA
Cuts at: 4897 4897
Size: 6536
BstEII G'GTnAC_C
Cuts at: 696 1821 696
Size: 1125 5411
BstXI CCAn_nnnn'nTGG
Cuts at: 2619 2832 3363 2619
Size: 213 531 5792
Fragments arranged by size:
5792 531 213
BstYI r'GATC_y
Cuts at: 1 1851 1857 2161 2228 2252 3561 4028 4051
Size: 1850 6 304 67 24 1309 467 23
Cuts at: 4051 4386 4632 4938 4950 5036 6088 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1850 1309 1052 467 449 335 306 304
246 86 67 24 23 12 6
Bsu36I CC'TnA_GG
Cuts at: 2561 2561
Size: 6536
BtgI C'CryG_G
Cuts at: 107 143 2270 107
Size: 36 2127 4373
Fragments arranged by size:
4373 2127 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4486 4667 4830 4486
Size: 181 163 6192
Fragments arranged by size:
6192 181 163
BtsI GCAGTG_nn'
Cuts at: 282 312 996 1618 1671 1927 3422 3512 3779
Size: 30 684 622 53 256 1495 90 267
Cuts at: 3779 282
Size: 3039
Fragments arranged by size:
3039 1495 684 622 267 256 90 53
30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 962 997 2735 2770 2964 2999 962
Size: 35 1738 35 194 35 4499
Fragments arranged by size:
4499 1738 194 35 35 35
DraI TTT'AAA
Cuts at: 3535 3535
Size: 6536
DrdI GACnn_nn'nnGTC
Cuts at: 4242 5586 4242
Size: 1344 5192
EaeI y'GGCC_r
Cuts at: 752 1659 1801 2312 2756 2955 3333 3425 4121
Size: 907 142 511 444 199 378 92 696
Cuts at: 4121 4295 4686 4713 6058 752
Size: 174 391 27 1345 1230
Fragments arranged by size:
1345 1230 907 696 511 444 391 378
199 174 142 92 27
EagI C'GGCC_G
Cuts at: 4121 4121
Size: 6536
EarI CTCTTCn'nnn_
Cuts at: 1727 2588 4559 4769 1727
Size: 861 1971 210 3494
Fragments arranged by size:
3494 1971 861 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1116 2154 2694 3172 4744 5486 5632 6346 1116
Size: 1038 540 478 1572 742 146 714 1306
Fragments arranged by size:
1572 1306 1038 742 714 540 478 146
EcoICRI GAG'CTC
Cuts at: 2690 3548 2690
Size: 858 5678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 350 484 634 901 1309 1384 1851 2551
Size: 193 134 150 267 408 75 467 700
Cuts at: 2551 2595 2600 2641 2667 2755 3466 4361 4793
Size: 44 5 41 26 88 711 895 432
Cuts at: 4793 5146 5808 157
Size: 353 662 885
Fragments arranged by size:
895 885 711 700 662 467 432 408
353 267 193 150 134 88 75 44
41 26 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1830 2857 3448 1830
Size: 1027 591 4918
Fragments arranged by size:
4918 1027 591
EcoO109I rG'GnC_Cy
Cuts at: 822 1424 1622 2829 3147 3675 822
Size: 602 198 1207 318 528 3683
Fragments arranged by size:
3683 1207 602 528 318 198
EcoRI G'AATT_C
Cuts at: 2605 3540 2605
Size: 935 5601
EcoRV GAT'ATC
Cuts at: 1132 1132
Size: 6536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2046 2322 2883 4555 2046
Size: 276 561 1672 4027
Fragments arranged by size:
4027 1672 561 276
FspI TGC'GCA
Cuts at: 2498 3163 4317 2498
Size: 665 1154 4717
Fragments arranged by size:
4717 1154 665
HaeII r_GCGC'y
Cuts at: 1528 2096 3064 3125 3468 3970 4218 5448 6219
Size: 568 968 61 343 502 248 1230 771
Cuts at: 6219 1528
Size: 1845
Fragments arranged by size:
1845 1230 968 771 568 502 343 248
61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1408 1440 1704 1736 3451 3483 4378 4408 4410
Size: 32 264 32 1715 32 895 30 2
Cuts at: 4410 4440 1408
Size: 30 3504
Fragments arranged by size:
3504 1715 895 264 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3575 3575
Size: 6536
HindIII A'AGCT_T
Cuts at: 1312 2149 1312
Size: 837 5699
KasI G'GCGC_C
Cuts at: 3966 4214 3966
Size: 248 6288
KpnI G_GTAC'C
Cuts at: 1057 3556 1057
Size: 2499 4037
MluI A'CGCG_T
Cuts at: 3113 5969 3113
Size: 2856 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 460 3165 5295 5479 460
Size: 2705 2130 184 1517
Fragments arranged by size:
2705 2130 1517 184
MscI TGG'CCA
Cuts at: 2314 2758 2957 4297 2314
Size: 444 199 1340 4553
Fragments arranged by size:
4553 1340 444 199
MslI CAynn'nnrTG
Cuts at: 4525 4652 4525
Size: 127 6409
MspA1I CmG'CkG
Cuts at: 119 255 299 350 540 735 1882 2133 2446
Size: 136 44 51 190 195 1147 251 313
Cuts at: 2446 2485 2765 2788 3024 3264 3411 3647 3961
Size: 39 280 23 236 240 147 236 314
Cuts at: 3961 4321 5105 5350 119
Size: 360 784 245 1305
Fragments arranged by size:
1305 1147 784 360 314 313 280 251
245 240 236 236 195 190 147 136
51 44 39 23
NaeI GCC'GGC
Cuts at: 1890 2041 4717 1890
Size: 151 2676 3709
Fragments arranged by size:
3709 2676 151
NarI GG'CG_CC
Cuts at: 3967 4215 3967
Size: 248 6288
NcoI C'CATG_G
Cuts at: 107 143 2270 107
Size: 36 2127 4373
Fragments arranged by size:
4373 2127 36
NgoMIV G'CCGG_C
Cuts at: 1888 2039 4715 1888
Size: 151 2676 3709
Fragments arranged by size:
3709 2676 151
NsiI A_TGCA'T
Cuts at: 2891 2891
Size: 6536
NspI r_CATG'y
Cuts at: 2893 3589 4089 5692 2893
Size: 696 500 1603 3737
Fragments arranged by size:
3737 1603 696 500
PasI CC'CwG_GG
Cuts at: 180 482 2400 2709 2902 180
Size: 302 1918 309 193 3814
Fragments arranged by size:
3814 1918 309 302 193
PciI A'CATG_T
Cuts at: 5688 5688
Size: 6536
PflMI CCAn_nnn'nTGG
Cuts at: 669 2400 2765 3096 3267 669
Size: 1731 365 331 171 3938
Fragments arranged by size:
3938 1731 365 331 171
PfoI T'CCnGG_A
Cuts at: 1966 2683 1966
Size: 717 5819
PmeI GTTT'AAAC
Cuts at: 3535 3535
Size: 6536
PmlI CAC'GTG
Cuts at: 3015 3015
Size: 6536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 4982
Size: 32 6504
PpuMI rG'GwC_Cy
Cuts at: 822 1424 3147 822
Size: 602 1723 4211
Fragments arranged by size:
4211 1723 602
PspOMI G'GGCC_C
Cuts at: 1622 1622
Size: 6536
PstI C_TGCA'G
Cuts at: 770 1613 1960 1994 2160 2881 3583 4268 770
Size: 843 347 34 166 721 702 685 3038
Fragments arranged by size:
3038 843 721 702 685 347 166 34
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6536
PvuII CAG'CTG
Cuts at: 255 540 1882 2133 2446 2485 2765 2788 3024
Size: 285 1342 251 313 39 280 23 236
Cuts at: 3024 3264 3961 4321 255
Size: 240 697 360 2470
Fragments arranged by size:
2470 1342 697 360 313 285 280 251
240 236 39 23
RsrII CG'GwC_CG
Cuts at: 4731 4731
Size: 6536
SacI G_AGCT'C
Cuts at: 2692 3550 2692
Size: 858 5678
SalI G'TCGA_C
Cuts at: 3573 3573
Size: 6536
SanDI GG'GwC_CC
Cuts at: 1424 1424
Size: 6536
SapI GCTCTTCn'nnn_
Cuts at: 4559 4769 4559
Size: 210 6326
SbfI CC_TGCA'GG
Cuts at: 2160 3583 2160
Size: 1423 5113
ScaI AGT'ACT
Cuts at: 2626 6053 2626
Size: 3427 3109
SexAI A'CCwGG_T
Cuts at: 1172 1172
Size: 6536
SfcI C'TryA_G
Cuts at: 33 766 1609 1956 1983 1990 2156 2540 2877
Size: 733 843 347 27 7 166 384 337
Cuts at: 2877 3579 4264 5232 5423 33
Size: 702 685 968 191 1146
Fragments arranged by size:
1146 968 843 733 702 685 384 347
337 191 166 27 7
SfoI GGC'GCC
Cuts at: 3968 4216 3968
Size: 248 6288
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6536
SmaI CCC'GGG
Cuts at: 2098 3404 3558 2098
Size: 1306 154 5076
Fragments arranged by size:
5076 1306 154
SmlI C'TyrA_G
Cuts at: 656 1075 2020 2278 2698 3691 4606 5320 5582
Size: 419 945 258 420 993 915 714 262
Cuts at: 5582 656
Size: 1610
Fragments arranged by size:
1610 993 945 915 714 420 419 262
258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6536
SphI G_CATG'C
Cuts at: 2893 3589 4089 2893
Size: 696 500 5340
Fragments arranged by size:
5340 696 500
StuI AGG'CCT
Cuts at: 2512 2512
Size: 6536
StyI C'CwwG_G
Cuts at: 107 143 1332 2270 2450 2549 2825 3670 4024
Size: 36 1189 938 180 99 276 845 354
Cuts at: 4024 107
Size: 2619
Fragments arranged by size:
2619 1189 938 845 354 276 180 99
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 118 400 495 1658 1809 3271 3493 4119 4252
Size: 282 95 1163 151 1462 222 626 133
Cuts at: 4252 4711 4920 118
Size: 459 209 1734
Fragments arranged by size:
1734 1462 1163 626 459 282 222 209
151 133 95
TatI w'GTAC_w
Cuts at: 723 2178 2624 6051 723
Size: 1455 446 3427 1208
Fragments arranged by size:
3427 1455 1208 446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 453 485 1798 1830 3164 3196 6053 6085 453
Size: 32 1313 32 1334 32 2857 32 904
Fragments arranged by size:
2857 1334 1313 904 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1174 4333 1174
Size: 3159 3377
XbaI T'CTAG_A
Cuts at: 3567 3567
Size: 6536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1941 2344 3093 4031 1941
Size: 403 749 938 4446
Fragments arranged by size:
4446 938 749 403
XmaI C'CCGG_G
Cuts at: 2096 3402 3556 2096
Size: 1306 154 5076
Fragments arranged by size:
5076 1306 154
XmnI GAAnn'nnTTC
Cuts at: 2029 2413 2029
Size: 384 6152
ZraI GAC'GTC
Cuts at: 2741 2741
Size: 6536
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AgeI AleI AloI AscI BaeI BmgBI BmtI
BsiWI BspEI BssHII BstZ17I ClaI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI