Restriction map for
pF1KB4218
(Circular) (Six-base) MAPSORT of: pF1KB4218.seq Check: 1952 from: 1 to: 5507
pF1KB4218 5507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1607 1607
Size: 5507
AatII G_ACGT'C
Cuts at: 1439 1439
Size: 5507
AccI GT'mk_AC
Cuts at: 2171 2545 2171
Size: 374 5133
Acc65I G'GTAC_C
Cuts at: 541 2523 541
Size: 1982 3525
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2428 3332 3764 4117 4779 2428
Size: 904 432 353 662 3156
Fragments arranged by size:
3156 904 662 432 353
AflIII A'CryG_T
Cuts at: 2859 4659 4940 2859
Size: 1800 281 3426
Fragments arranged by size:
3426 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1716 1716
Size: 5507
AleI CACnn'nnGTG
Cuts at: 748 748
Size: 5507
AlwNI CAG_nnn'CTG
Cuts at: 190 2812 4250 190
Size: 2622 1438 1447
Fragments arranged by size:
2622 1447 1438
ApaLI G'TGCA_C
Cuts at: 4345 4345
Size: 5507
ApoI r'AATT_y
Cuts at: 354 650 1172 2401 2511 4860 5116 354
Size: 296 522 1229 110 2349 256 745
Fragments arranged by size:
2349 1229 745 522 296 256 110
AseI AT'TA_AT
Cuts at: 20 989 20
Size: 969 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 179 299 862 1838 1893 1942 2290 2527 179
Size: 120 563 976 55 49 348 237 3159
Fragments arranged by size:
3159 976 563 348 237 120 55 49
AvrII C'CTAG_G
Cuts at: 261 261
Size: 5507
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 533 566 533
Size: 33 5474
BamHI G'GATC_C
Cuts at: 760 2532 760
Size: 1772 3735
BanI G'GyrC_C
Cuts at: 541 1549 1595 1702 1833 2286 2329 2523 2937
Size: 1008 46 107 131 453 43 194 414
Cuts at: 2937 3185 3220 541
Size: 248 35 2828
Fragments arranged by size:
2828 1008 453 414 248 194 131 107
46 43 35
BanII G_rGCy'C
Cuts at: 410 1943 2521 3551 410
Size: 1533 578 1030 2366
Fragments arranged by size:
2366 1533 1030 578
BbeI G_GCGC'C
Cuts at: 1706 2941 3189 1706
Size: 1235 248 4024
Fragments arranged by size:
4024 1235 248
BbsI GAAGACnn'nnnn_
Cuts at: 278 278
Size: 5507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2174 2208 2174
Size: 34 5473
BciVI GTATCCnnnnn_n'
Cuts at: 1225 3405 4461 5507 1225
Size: 2180 1056 1046 1225
Fragments arranged by size:
2180 1225 1056 1046
BclI T'GATC_A
Cuts at: 489 3027 489
Size: 2538 2969
BfrBI ATG'CAT
Cuts at: 958 958
Size: 5507
BglII A'GATC_T
Cuts at: 3022 5059 3022
Size: 2037 3470
BlpI GC'TnA_GC
Cuts at: 1996 2619 1996
Size: 623 4884
Bme1580I G_kGCm'C
Cuts at: 126 1838 2291 3132 3225 4349 126
Size: 1712 453 841 93 1124 1284
Fragments arranged by size:
1712 1284 1124 841 453 93
BmgBI CAC'GTC
Cuts at: 2322 2322
Size: 5507
BmrI ACTGGGnnnn_n'
Cuts at: 359 2719 2800 3135 5279 359
Size: 2360 81 335 2144 587
Fragments arranged by size:
2360 2144 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 1203 3874 142
Size: 1061 2671 1775
Fragments arranged by size:
2671 1775 1061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1253 1253
Size: 5507
Bpu10I CC'TnA_GC
Cuts at: 905 905
Size: 5507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 1912 2683 3562 4276 4574 563
Size: 1349 771 879 714 298 1496
Fragments arranged by size:
1496 1349 879 771 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1186 1623 5211 1186
Size: 437 3588 1482
Fragments arranged by size:
3588 1482 437
BsaAI yAC'GTr
Cuts at: 292 2085 2860 292
Size: 1793 775 2939
Fragments arranged by size:
2939 1793 775
BsaBI GATnn'nnATC
Cuts at: 6 3021 5058 6
Size: 3015 2037 455
Fragments arranged by size:
3015 2037 455
BsaHI Gr'CG_yC
Cuts at: 1436 1703 1766 2938 3186 3888 5384 1436
Size: 267 63 1172 248 702 1496 1559
Fragments arranged by size:
1559 1496 1172 702 267 248 63
BsaWI w'CCGG_w
Cuts at: 2696 3217 3895 4306 4453 4727 2696
Size: 521 678 411 147 274 3476
Fragments arranged by size:
3476 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 1675 1705 2089 2119 268
Size: 30 1377 30 384 30 3656
Fragments arranged by size:
3656 1377 384 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 2048 2268 292
Size: 1756 220 3531
Fragments arranged by size:
3531 1756 220
BseYI C'CCAG_C
Cuts at: 168 267 2108 2932 3690 4355 4763 168
Size: 99 1841 824 758 665 408 912
Fragments arranged by size:
1841 912 824 758 665 408 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 1495 1806 2065 2268 182
Size: 1313 311 259 203 3421
Fragments arranged by size:
3421 1313 311 259 203
BsiEI CG_ry'CG
Cuts at: 104 1847 3049 3095 4325 104
Size: 1743 1202 46 1230 1286
Fragments arranged by size:
1743 1286 1230 1202 46
BsiHKAI G_wGCw'C
Cuts at: 579 2090 2225 2521 3299 3489 4349 579
Size: 1511 135 296 778 190 860 1737
Fragments arranged by size:
1737 1511 860 778 296 190 135
BsmI GAATG_Cn'
Cuts at: 1188 1188
Size: 5507
BsmBI CGTCTCn'nnnn_
Cuts at: 377 377
Size: 5507
Bsp1286I G_dGCh'C
Cuts at: 126 410 579 1838 1943 2090 2225 2291 2521
Size: 284 169 1259 105 147 135 66 230
Cuts at: 2521 3132 3225 3299 3489 3551 4349 126
Size: 611 93 74 190 62 798 1284
Fragments arranged by size:
1284 1259 798 611 284 230 190 169
147 135 105 93 74 66 62
BspHI T'CATG_A
Cuts at: 2436 3939 2436
Size: 1503 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 1607 2126 2557 2771 3073 3454 175
Size: 1432 519 431 214 302 381 2228
Fragments arranged by size:
2228 1432 519 431 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2124 2327 3799 3853 5337 5502 50
Size: 2074 203 1472 54 1484 165 55
Fragments arranged by size:
2074 1484 1472 203 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3419 3419
Size: 5507
BsrFI r'CCGG_y
Cuts at: 1592 3505 3686 1592
Size: 1913 181 3413
Fragments arranged by size:
3413 1913 181
BssSI C'ACGA_G
Cuts at: 3778 4486 3778
Size: 708 4799
BstAPI GCAn_nnn'nTGC
Cuts at: 2884 2884
Size: 5507
BstBI TT'CG_AA
Cuts at: 2475 3868 2475
Size: 1393 4114
BstEII G'GTnAC_C
Cuts at: 1863 1863
Size: 5507
BstXI CCAn_nnnn'nTGG
Cuts at: 1231 1875 1231
Size: 644 4863
BstYI r'GATC_y
Cuts at: 1 760 909 2532 2999 3022 3357 3603 3909
Size: 759 149 1623 467 23 335 246 306
Cuts at: 3909 3921 4007 5059 1
Size: 12 86 1052 449
Fragments arranged by size:
1623 1052 759 467 449 335 306 246
149 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1654 2129 1654
Size: 475 5032
BtgI C'CryG_G
Cuts at: 107 1713 1934 107
Size: 1606 221 3680
Fragments arranged by size:
3680 1606 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 755 2750 755
Size: 1995 3512
DraI TTT'AAA
Cuts at: 2506 2506
Size: 5507
DrdI GACnn_nn'nnGTC
Cuts at: 1155 3213 4557 1155
Size: 2058 1344 2105
Fragments arranged by size:
2105 2058 1344
EaeI y'GGCC_r
Cuts at: 1638 1844 2012 2066 3092 3266 3657 3684 5029
Size: 206 168 54 1026 174 391 27 1345
Cuts at: 5029 1638
Size: 2116
Fragments arranged by size:
2116 1345 1026 391 206 174 168 54
27
EagI C'GGCC_G
Cuts at: 1844 3092 1844
Size: 1248 4259
EarI CTCTTCn'nnn_
Cuts at: 2212 2218 3530 3740 2212
Size: 6 1312 210 3979
Fragments arranged by size:
3979 1312 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3715 4457 4603 5317 3715
Size: 742 146 714 3905
Fragments arranged by size:
3905 742 714 146
EcoICRI GAG'CTC
Cuts at: 2519 2519
Size: 5507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1253 2428 3332 3764 4117 4779 1253
Size: 1175 904 432 353 662 1981
Fragments arranged by size:
1981 1175 904 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 258 1674 1718 2296 2336 2646 258
Size: 1416 44 578 40 310 3119
Fragments arranged by size:
3119 1416 578 310 44 40
EcoRI G'AATT_C
Cuts at: 1172 2511 1172
Size: 1339 4168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5507
FspI TGC'GCA
Cuts at: 1981 3288 1981
Size: 1307 4200
FspAI rTGC'GCAy
Cuts at: 1981 1981
Size: 5507
HaeII r_GCGC'y
Cuts at: 1532 1706 1744 2941 3189 4419 5190 1532
Size: 174 38 1197 248 1230 771 1849
Fragments arranged by size:
1849 1230 1197 771 248 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 725 745 757 777 1213 1245 1528
Size: 32 425 20 12 20 436 32 283
Cuts at: 1528 1560 1702 1734 1876 1908 2089 2121 3349
Size: 32 142 32 142 32 181 32 1228
Cuts at: 3349 3379 3381 3411 268
Size: 30 2 30 2364
Fragments arranged by size:
2364 1228 436 425 283 181 142 142
32 32 32 32 32 32 30 30
20 20 12 2
HincII GTy'rAC
Cuts at: 1566 2546 1566
Size: 980 4527
KasI G'GCGC_C
Cuts at: 1702 2937 3185 1702
Size: 1235 248 4024
Fragments arranged by size:
4024 1235 248
KpnI G_GTAC'C
Cuts at: 545 2527 545
Size: 1982 3525
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 531 573 785 4266 4450 531
Size: 42 212 3481 184 1588
Fragments arranged by size:
3481 1588 212 184 42
MscI TGG'CCA
Cuts at: 1640 3268 1640
Size: 1628 3879
MslI CAynn'nnrTG
Cuts at: 748 799 1420 3496 3623 748
Size: 51 621 2076 127 2632
Fragments arranged by size:
2632 2076 621 127 51
MspA1I CmG'CkG
Cuts at: 1715 1800 1923 2618 2932 3292 4076 4321 1715
Size: 85 123 695 314 360 784 245 2901
Fragments arranged by size:
2901 784 695 360 314 245 123 85
NaeI GCC'GGC
Cuts at: 3688 3688
Size: 5507
NarI GG'CG_CC
Cuts at: 1703 2938 3186 1703
Size: 1235 248 4024
Fragments arranged by size:
4024 1235 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5507
NgoMIV G'CCGG_C
Cuts at: 3686 3686
Size: 5507
NsiI A_TGCA'T
Cuts at: 960 960
Size: 5507
NspI r_CATG'y
Cuts at: 2560 3060 4663 2560
Size: 500 1603 3404
Fragments arranged by size:
3404 1603 500
PciI A'CATG_T
Cuts at: 4659 4659
Size: 5507
PflMI CCAn_nnn'nTGG
Cuts at: 1000 1000
Size: 5507
PfoI T'CCnGG_A
Cuts at: 883 2383 883
Size: 1500 4007
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PmlI CAC'GTG
Cuts at: 292 2085 292
Size: 1793 3714
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 5475
PpuMI rG'GwC_Cy
Cuts at: 258 1718 2296 2336 258
Size: 1460 578 40 3429
Fragments arranged by size:
3429 1460 578 40
PstI C_TGCA'G
Cuts at: 1805 2554 3239 1805
Size: 749 685 4073
Fragments arranged by size:
4073 749 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 1923 2932 3292 1923
Size: 1009 360 4138
Fragments arranged by size:
4138 1009 360
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 2521 2521
Size: 5507
SacII CC_GC'GG
Cuts at: 1716 1716
Size: 5507
SalI G'TCGA_C
Cuts at: 2544 2544
Size: 5507
SanDI GG'GwC_CC
Cuts at: 1718 2296 2336 1718
Size: 578 40 4889
Fragments arranged by size:
4889 578 40
SapI GCTCTTCn'nnn_
Cuts at: 2212 3530 3740 2212
Size: 1318 210 3979
Fragments arranged by size:
3979 1318 210
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 5024 5024
Size: 5507
SexAI A'CCwGG_T
Cuts at: 1859 2174 1859
Size: 315 5192
SfcI C'TryA_G
Cuts at: 33 1678 1801 2550 3235 4203 4394 33
Size: 1645 123 749 685 968 191 1146
Fragments arranged by size:
1645 1146 968 749 685 191 123
SfoI GGC'GCC
Cuts at: 1704 2939 3187 1704
Size: 1235 248 4024
Fragments arranged by size:
4024 1235 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 301 1840 1944 2529 301
Size: 1539 104 585 3279
Fragments arranged by size:
3279 1539 585 104
SmlI C'TyrA_G
Cuts at: 578 1927 2662 3577 4291 4553 578
Size: 1349 735 915 714 262 1532
Fragments arranged by size:
1532 1349 915 735 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5507
SphI G_CATG'C
Cuts at: 2560 3060 2560
Size: 500 5007
SrfI GCCC'GGGC
Cuts at: 1944 1944
Size: 5507
SspI AAT'ATT
Cuts at: 155 1062 155
Size: 907 4600
StyI C'CwwG_G
Cuts at: 107 261 876 1613 1868 2641 2995 107
Size: 154 615 737 255 773 354 2619
Fragments arranged by size:
2619 773 737 615 354 255 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 395 1407 1477 3090 3223 3682 3891 395
Size: 1012 70 1613 133 459 209 2011
Fragments arranged by size:
2011 1613 1012 459 209 133 70
TatI w'GTAC_w
Cuts at: 1355 1409 5022 1355
Size: 54 3613 1840
Fragments arranged by size:
3613 1840 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5024 5056 5024
Size: 32 5475
Tth111I GACn'n_nGTC
Cuts at: 2340 3304 2340
Size: 964 4543
XbaI T'CTAG_A
Cuts at: 507 2538 507
Size: 2031 3476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 3002 2022
Size: 980 4527
XmaI C'CCGG_G
Cuts at: 299 1838 1942 2527 299
Size: 1539 104 585 3279
Fragments arranged by size:
3279 1539 585 104
XmnI GAAnn'nnTTC
Cuts at: 968 1306 968
Size: 338 5169
ZraI GAC'GTC
Cuts at: 1437 1437
Size: 5507
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AlfI AloI ApaI AscI BbvCI
BglI BmtI BsiWI BspEI BsrGI BssHII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PshAI PsiI
PspOMI PspXI PsrI SfiI SgrAI SnaBI StuI SwaI
XhoI