Restriction map for
pF1KB4219
(Circular) (Six-base) MAPSORT of: pF1KB4219.seq Check: 9951 from: 1 to: 5936
pF1KB4219 5936 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1337 1337
Size: 5936
AccI GT'mk_AC
Cuts at: 350 1852 2974 350
Size: 1502 1122 3312
Fragments arranged by size:
3312 1502 1122
Acc65I G'GTAC_C
Cuts at: 2952 2952
Size: 5936
AclI AA'CG_TT
Cuts at: 5776 5776
Size: 5936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 3761 4193 4546 5208 1051
Size: 2710 432 353 662 1779
Fragments arranged by size:
2710 1779 662 432 353
AflIII A'CryG_T
Cuts at: 1509 1949 3288 5088 5369 1509
Size: 440 1339 1800 281 2076
Fragments arranged by size:
2076 1800 1339 440 281
AhdI GACnn_n'nnGTC
Cuts at: 1017 1017
Size: 5936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 906 1410 2315 906
Size: 504 905 4527
Fragments arranged by size:
4527 905 504
AlwNI CAG_nnn'CTG
Cuts at: 687 1533 2736 3241 4679 687
Size: 846 1203 505 1438 1944
Fragments arranged by size:
1944 1438 1203 846 505
ApaI G_GGCC'C
Cuts at: 704 2898 704
Size: 2194 3742
ApaLI G'TGCA_C
Cuts at: 598 4774 598
Size: 4176 1760
ApoI r'AATT_y
Cuts at: 1986 2593 2940 5289 5545 1986
Size: 607 347 2349 256 2377
Fragments arranged by size:
2377 2349 607 347 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
AvaI C'yCGr_G
Cuts at: 335 716 878 958 1939 2149 2956 335
Size: 381 162 80 981 210 807 3315
Fragments arranged by size:
3315 981 807 381 210 162 80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 524 557 524
Size: 33 5903
BamHI G'GATC_C
Cuts at: 1874 2061 2961 1874
Size: 187 900 4849
Fragments arranged by size:
4849 900 187
BanI G'GyrC_C
Cuts at: 2291 2871 2952 3366 3614 3649 2291
Size: 580 81 414 248 35 4578
Fragments arranged by size:
4578 580 414 248 81 35
BanII G_rGCy'C
Cuts at: 274 704 2138 2278 2898 2950 3980 274
Size: 430 1434 140 620 52 1030 2230
Fragments arranged by size:
2230 1434 1030 620 430 140 52
BbeI G_GCGC'C
Cuts at: 3370 3618 3370
Size: 248 5688
BbsI GAAGACnn'nnnn_
Cuts at: 186 758 1016 2563 186
Size: 572 258 1547 3559
Fragments arranged by size:
3559 1547 572 258
BbvCI CC'TCA_GC
Cuts at: 1378 2459 2927 1378
Size: 1081 468 4387
Fragments arranged by size:
4387 1081 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 811 845 924 958 1731 1765 2149 2183 2229
Size: 34 79 34 773 34 384 34 46
Cuts at: 2229 2263 811
Size: 34 4484
Fragments arranged by size:
4484 773 384 79 46 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3834 4890 5936 3834
Size: 1056 1046 3834
Fragments arranged by size:
3834 1056 1046
BclI T'GATC_A
Cuts at: 2346 3456 2346
Size: 1110 4826
BglII A'GATC_T
Cuts at: 1391 2162 3451 5488 1391
Size: 771 1289 2037 1839
Fragments arranged by size:
2037 1839 1289 771
BlpI GC'TnA_GC
Cuts at: 173 3048 173
Size: 2875 3061
Bme1580I G_kGCm'C
Cuts at: 602 704 2898 3561 3654 4778 602
Size: 102 2194 663 93 1124 1760
Fragments arranged by size:
2194 1760 1124 663 102 93
BmgBI CAC'GTC
Cuts at: 1952 1952
Size: 5936
BmrI ACTGGGnnnn_n'
Cuts at: 3148 3229 3564 5708 3148
Size: 81 335 2144 3376
Fragments arranged by size:
3376 2144 335 81
BmtI G_CTAG'C
Cuts at: 559 559
Size: 5936
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1413 4303 1413
Size: 2890 3046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1333 2152 2431 2635 907
Size: 426 819 279 204 4208
Fragments arranged by size:
4208 819 426 279 204
Bpu10I CC'TnA_GC
Cuts at: 1378 1966 2303 2459 2675 2927 1378
Size: 588 337 156 216 252 4387
Fragments arranged by size:
4387 588 337 252 216 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1258 3112 3991 4705 5003 184
Size: 1074 1854 879 714 298 1117
Fragments arranged by size:
1854 1117 1074 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 868 1842 2603 5640 868
Size: 974 761 3037 1164
Fragments arranged by size:
3037 1164 974 761
BsaAI yAC'GTr
Cuts at: 1512 3289 1512
Size: 1777 4159
BsaBI GATnn'nnATC
Cuts at: 6 1480 2713 3450 5487 6
Size: 1474 1233 737 2037 455
Fragments arranged by size:
2037 1474 1233 737 455
BsaHI Gr'CG_yC
Cuts at: 254 1334 3367 3615 4317 5813 254
Size: 1080 2033 248 702 1496 377
Fragments arranged by size:
2033 1496 1080 702 377 248
BsaWI w'CCGG_w
Cuts at: 3125 3646 4324 4735 4882 5156 3125
Size: 521 678 411 147 274 3905
Fragments arranged by size:
3905 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 938 968 1027 1057 304
Size: 30 604 30 59 30 5183
Fragments arranged by size:
5183 604 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2494 2503 2875 2494
Size: 9 372 5555
Fragments arranged by size:
5555 372 9
BseYI C'CCAG_C
Cuts at: 377 456 471 571 3361 4119 4784 5192 377
Size: 79 15 100 2790 758 665 408 1121
Fragments arranged by size:
2790 1121 758 665 408 100 79 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 5936
BsiEI CG_ry'CG
Cuts at: 104 169 783 789 1490 2475 3478 3524 4754
Size: 65 614 6 701 985 1003 46 1230
Cuts at: 4754 104
Size: 1286
Fragments arranged by size:
1286 1230 1003 985 701 614 65 46
6
BsiHKAI G_wGCw'C
Cuts at: 332 602 857 959 1238 2138 2702 2789 2950
Size: 270 255 102 279 900 564 87 161
Cuts at: 2950 3728 3918 4778 332
Size: 778 190 860 1490
Fragments arranged by size:
1490 900 860 778 564 279 270 255
190 161 102 87
BsmBI CGTCTCn'nnnn_
Cuts at: 1341 1341
Size: 5936
Bsp1286I G_dGCh'C
Cuts at: 274 332 602 704 857 959 1238 2138 2278
Size: 58 270 102 153 102 279 900 140
Cuts at: 2278 2702 2789 2898 2950 3561 3654 3728 3918
Size: 424 87 109 52 611 93 74 190
Cuts at: 3918 3980 4778 274
Size: 62 798 1432
Fragments arranged by size:
1432 900 798 611 424 279 270 190
153 140 109 102 102 93 87 74
62 58 52
BspHI T'CATG_A
Cuts at: 2193 2516 4368 2193
Size: 323 1852 3761
Fragments arranged by size:
3761 1852 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 180 494 672 872 2123 2986 3200 3502 3883
Size: 314 178 200 1251 863 214 302 381
Cuts at: 3883 180
Size: 2233
Fragments arranged by size:
2233 1251 863 381 314 302 214 200
178
BsrBI CCG'CTC
Cuts at: 50 4228 4282 5766 5931 50
Size: 4178 54 1484 165 55
Fragments arranged by size:
4178 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 471 2702 3848 471
Size: 2231 1146 2559
Fragments arranged by size:
2559 2231 1146
BsrFI r'CCGG_y
Cuts at: 168 1591 3934 4115 168
Size: 1423 2343 181 1989
Fragments arranged by size:
2343 1989 1423 181
BsrGI T'GTAC_A
Cuts at: 1053 1053
Size: 5936
BssSI C'ACGA_G
Cuts at: 4207 4915 4207
Size: 708 5228
BstAPI GCAn_nnn'nTGC
Cuts at: 687 1095 3313 687
Size: 408 2218 3310
Fragments arranged by size:
3310 2218 408
BstBI TT'CG_AA
Cuts at: 4297 4297
Size: 5936
BstXI CCAn_nnnn'nTGG
Cuts at: 513 1248 2415 513
Size: 735 1167 4034
Fragments arranged by size:
4034 1167 735
BstYI r'GATC_y
Cuts at: 1 794 1391 1475 1529 1874 2061 2162 2382
Size: 793 597 84 54 345 187 101 220
Cuts at: 2382 2961 3428 3451 3786 4032 4338 4350 4436
Size: 579 467 23 335 246 306 12 86
Cuts at: 4436 5488 1
Size: 1052 449
Fragments arranged by size:
1052 793 597 579 467 449 345 335
306 246 220 187 101 86 84 54
23 12
BstZ17I GTA'TAC
Cuts at: 1853 1853
Size: 5936
BtgI C'CryG_G
Cuts at: 107 178 578 1520 1572 1687 1955 2328 2624
Size: 71 400 942 52 115 268 373 296
Cuts at: 2624 2820 107
Size: 196 3223
Fragments arranged by size:
3223 942 400 373 296 268 196 115
71 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1485 1734 2295 2722 3886 4067 4230 1485
Size: 249 561 427 1164 181 163 3191
Fragments arranged by size:
3191 1164 561 427 249 181 163
BtsI GCAGTG_nn'
Cuts at: 1209 3179 1209
Size: 1970 3966
DraI TTT'AAA
Cuts at: 2935 2935
Size: 5936
DrdI GACnn_nn'nnGTC
Cuts at: 1339 2799 3642 4986 1339
Size: 1460 843 1344 2289
Fragments arranged by size:
2289 1460 1344 843
EaeI y'GGCC_r
Cuts at: 123 503 606 1430 1487 1658 1805 1958 2405
Size: 380 103 824 57 171 147 153 447
Cuts at: 2405 3521 3695 4086 4113 5458 123
Size: 1116 174 391 27 1345 601
Fragments arranged by size:
1345 1116 824 601 447 391 380 174
171 153 147 103 57 27
EagI C'GGCC_G
Cuts at: 1487 3521 1487
Size: 2034 3902
EarI CTCTTCn'nnn_
Cuts at: 840 959 1298 1321 3959 4169 840
Size: 119 339 23 2638 210 2607
Fragments arranged by size:
2638 2607 339 210 119 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 763 4144 4886 5032 5746 763
Size: 3381 742 146 714 953
Fragments arranged by size:
3381 953 742 714 146
EcoICRI GAG'CTC
Cuts at: 2136 2948 2136
Size: 812 5124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1051 1333 2152 2431 2635 3761 4193 4546
Size: 144 282 819 279 204 1126 432 353
Cuts at: 4546 5208 907
Size: 662 1635
Fragments arranged by size:
1635 1126 819 662 432 353 282 279
204 144
EcoNI CCTnn'n_nnAGG
Cuts at: 504 868 997 1348 1939 2617 2857 504
Size: 364 129 351 591 678 240 3583
Fragments arranged by size:
3583 678 591 364 351 240 129
EcoO109I rG'GnC_Cy
Cuts at: 315 393 684 1035 1710 2442 2733 2894 2895
Size: 78 291 351 675 732 291 161 1
Cuts at: 2895 3075 315
Size: 180 3176
Fragments arranged by size:
3176 732 675 351 291 291 180 161
78 1
EcoRI G'AATT_C
Cuts at: 2593 2940 2593
Size: 347 5589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1332 3955 1332
Size: 2623 3313
FspI TGC'GCA
Cuts at: 3717 3717
Size: 5936
HaeII r_GCGC'y
Cuts at: 3370 3618 4848 5619 3370
Size: 248 1230 771 3687
Fragments arranged by size:
3687 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 358 390 1003 1027 1035 1059 1321
Size: 32 22 32 613 24 8 24 262
Cuts at: 1321 1353 1936 1968 2338 2370 2489 2521 3778
Size: 32 583 32 370 32 119 32 1257
Cuts at: 3778 3808 3810 3840 304
Size: 30 2 30 2400
Fragments arranged by size:
2400 1257 613 583 370 262 119 32
32 32 32 32 32 30 30 24
24 22 8 2
HincII GTy'rAC
Cuts at: 2975 2975
Size: 5936
KasI G'GCGC_C
Cuts at: 3366 3614 3366
Size: 248 5688
KpnI G_GTAC'C
Cuts at: 2956 2956
Size: 5936
MluI A'CGCG_T
Cuts at: 5369 5369
Size: 5936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 825 890 1865 2801 4695 4879 825
Size: 65 975 936 1894 184 1882
Fragments arranged by size:
1894 1882 975 936 184 65
MscI TGG'CCA
Cuts at: 505 608 1432 1807 1960 2407 3697 505
Size: 103 824 375 153 447 1290 2744
Fragments arranged by size:
2744 1290 824 447 375 153 103
MslI CAynn'nnrTG
Cuts at: 595 1328 1654 1954 2707 3925 4052 595
Size: 733 326 300 753 1218 127 2479
Fragments arranged by size:
2479 1218 753 733 326 300 127
MspA1I CmG'CkG
Cuts at: 180 325 381 586 612 890 1029 1062 1574
Size: 145 56 205 26 278 139 33 512
Cuts at: 1574 2822 3047 3361 3721 4505 4750 180
Size: 1248 225 314 360 784 245 1366
Fragments arranged by size:
1366 1248 784 512 360 314 278 245
225 205 145 139 56 33 26
NaeI GCC'GGC
Cuts at: 4117 4117
Size: 5936
NarI GG'CG_CC
Cuts at: 3367 3615 3367
Size: 248 5688
NcoI C'CATG_G
Cuts at: 107 1687 1955 2624 107
Size: 1580 268 669 3419
Fragments arranged by size:
3419 1580 669 268
NdeI CA'TA_TG
Cuts at: 1551 1551
Size: 5936
NgoMIV G'CCGG_C
Cuts at: 4115 4115
Size: 5936
NheI G'CTAG_C
Cuts at: 555 555
Size: 5936
NspI r_CATG'y
Cuts at: 122 1074 1201 1974 2190 2379 2989 3489 5092
Size: 952 127 773 216 189 610 500 1603
Cuts at: 5092 122
Size: 966
Fragments arranged by size:
1603 966 952 773 610 500 216 189
127
PasI CC'CwG_GG
Cuts at: 704 2604 704
Size: 1900 4036
PciI A'CATG_T
Cuts at: 5088 5088
Size: 5936
PflMI CCAn_nnn'nTGG
Cuts at: 543 1005 1137 1884 2454 2552 543
Size: 462 132 747 570 98 3927
Fragments arranged by size:
3927 747 570 462 132 98
PfoI T'CCnGG_A
Cuts at: 428 1349 2356 2852 428
Size: 921 1007 496 3512
Fragments arranged by size:
3512 1007 921 496
PmeI GTTT'AAAC
Cuts at: 2935 2935
Size: 5936
PmlI CAC'GTG
Cuts at: 1512 1512
Size: 5936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1256 1288 4382 4414 1256
Size: 32 3094 32 2778
Fragments arranged by size:
3094 2778 32 32
PpuMI rG'GwC_Cy
Cuts at: 393 684 1035 1710 2442 2733 393
Size: 291 351 675 732 291 3596
Fragments arranged by size:
3596 732 675 351 291 291
PspOMI G'GGCC_C
Cuts at: 700 2894 700
Size: 2194 3742
PspXI vC'TCGA_Gb
Cuts at: 2149 2149
Size: 5936
PstI C_TGCA'G
Cuts at: 142 251 641 692 845 869 1067 1091 1979
Size: 109 390 51 153 24 198 24 888
Cuts at: 1979 2917 2983 3668 142
Size: 938 66 685 2410
Fragments arranged by size:
2410 938 888 685 390 198 153 109
66 51 24 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5936
PvuII CAG'CTG
Cuts at: 325 381 586 612 1029 1062 3361 3721 325
Size: 56 205 26 417 33 2299 360 2540
Fragments arranged by size:
2540 2299 417 360 205 56 33 26
RsrII CG'GwC_CG
Cuts at: 4131 4131
Size: 5936
SacI G_AGCT'C
Cuts at: 2138 2950 2138
Size: 812 5124
SacII CC_GC'GG
Cuts at: 181 1575 2823 181
Size: 1394 1248 3294
Fragments arranged by size:
3294 1394 1248
SalI G'TCGA_C
Cuts at: 2973 2973
Size: 5936
SapI GCTCTTCn'nnn_
Cuts at: 1298 3959 4169 1298
Size: 2661 210 3065
Fragments arranged by size:
3065 2661 210
SbfI CC_TGCA'GG
Cuts at: 251 641 2983 251
Size: 390 2342 3204
Fragments arranged by size:
3204 2342 390
ScaI AGT'ACT
Cuts at: 5453 5453
Size: 5936
SexAI A'CCwGG_T
Cuts at: 1130 1130
Size: 5936
SfcI C'TryA_G
Cuts at: 33 138 247 637 688 841 865 1063 1087
Size: 105 109 390 51 153 24 198 24
Cuts at: 1087 1975 2262 2913 2979 3664 4632 4823 33
Size: 888 287 651 66 685 968 191 1146
Fragments arranged by size:
1146 968 888 685 651 390 287 198
191 153 109 105 66 51 24 24
SfoI GGC'GCC
Cuts at: 3368 3616 3368
Size: 248 5688
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
SmaI CCC'GGG
Cuts at: 880 2958 880
Size: 2078 3858
SmlI C'TyrA_G
Cuts at: 199 1273 2149 3091 4006 4720 4982 199
Size: 1074 876 942 915 714 262 1153
Fragments arranged by size:
1153 1074 942 915 876 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5936
SphI G_CATG'C
Cuts at: 122 1974 2379 2989 3489 122
Size: 1852 405 610 500 2569
Fragments arranged by size:
2569 1852 610 500 405
StuI AGG'CCT
Cuts at: 293 2665 293
Size: 2372 3564
StyI C'CwwG_G
Cuts at: 107 500 1142 1433 1687 1955 2546 2624 2795
Size: 393 642 291 254 268 591 78 171
Cuts at: 2795 3070 3424 107
Size: 275 354 2619
Fragments arranged by size:
2619 642 591 393 354 291 275 268
254 171 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1336 2341 2489 3519 3652 4111 4320 1336
Size: 1005 148 1030 133 459 209 2952
Fragments arranged by size:
2952 1030 1005 459 209 148 133
TatI w'GTAC_w
Cuts at: 1053 5451 1053
Size: 4398 1538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 938 970 5453 5485 938
Size: 32 4483 32 1389
Fragments arranged by size:
4483 1389 32 32
Tth111I GACn'n_nGTC
Cuts at: 3733 3733
Size: 5936
XbaI T'CTAG_A
Cuts at: 2967 2967
Size: 5936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1131 3431 1131
Size: 2300 3636
XhoI C'TCGA_G
Cuts at: 2149 2149
Size: 5936
XmaI C'CCGG_G
Cuts at: 878 2956 878
Size: 2078 3858
XmnI GAAnn'nnTTC
Cuts at: 1315 1315
Size: 5936
ZraI GAC'GTC
Cuts at: 1335 1335
Size: 5936
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AloI AscI AvrII
BfrBI BglI BsiWI BsmI BspEI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NotI NruI NsiI PacI PshAI PsiI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI