Restriction map for
pF1KB4220
(Circular) (Six-base) MAPSORT of: pF1KB4220.seq Check: 7898 from: 1 to: 4154
pF1KB4220 4154 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 726 1192 726
Size: 466 3688
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 353 460 1979 2411 3158 3426 343
Size: 10 107 1519 432 747 268 1071
Fragments arranged by size:
1519 1071 747 432 268 107 10
AflII C'TTAA_G
Cuts at: 217 217
Size: 4154
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1043 1043
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 681 1459 3026 681
Size: 778 1567 1809
Fragments arranged by size:
1809 1567 778
ApaI G_GGCC'C
Cuts at: 1011 1011
Size: 4154
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 1158 3507 3763 1158
Size: 2349 256 1549
Fragments arranged by size:
2349 1549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 965 1174 965
Size: 209 3945
BamHI G'GATC_C
Cuts at: 1000 1179 1000
Size: 179 3975
BanI G'GyrC_C
Cuts at: 773 1170 1584 1832 1867 773
Size: 397 414 248 35 3060
Fragments arranged by size:
3060 414 397 248 35
BanII G_rGCy'C
Cuts at: 946 1011 1168 2198 946
Size: 65 157 1030 2902
Fragments arranged by size:
2902 1030 157 65
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbsI GAAGACnn'nnnn_
Cuts at: 942 942
Size: 4154
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2813 4154 2052
Size: 761 1341 2052
Fragments arranged by size:
2052 1341 761
BclI T'GATC_A
Cuts at: 1674 1674
Size: 4154
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 1011 1779 1872 2928 1011
Size: 768 93 1056 2237
Fragments arranged by size:
2237 1056 768 93
BmrI ACTGGGnnnn_n'
Cuts at: 744 846 1366 1447 1782 3926 744
Size: 102 520 81 335 2144 972
Fragments arranged by size:
2144 972 520 335 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 960 2521 960
Size: 1561 2593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1036
Size: 4154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 2209 2701 2999 1330
Size: 879 492 298 2485
Fragments arranged by size:
2485 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 394 3858 394
Size: 3464 690
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 1100 1668 3705 6
Size: 1094 568 2037 455
Fragments arranged by size:
2037 1094 568 455
BsaHI Gr'CG_yC
Cuts at: 1585 1833 2535 4031 1585
Size: 248 702 1496 1708
Fragments arranged by size:
1708 1496 702 248
BsaWI w'CCGG_w
Cuts at: 536 1343 1864 2542 2816 2963 3374 536
Size: 807 521 678 274 147 411 1316
Fragments arranged by size:
1316 807 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 651 681 651
Size: 30 4124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1132 1135 1144 1132
Size: 3 9 4142
Fragments arranged by size:
4142 9 3
BseYI C'CCAG_C
Cuts at: 750 1010 1579 2337 2914 3410 750
Size: 260 569 758 577 496 1494
Fragments arranged by size:
1494 758 577 569 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 735
Size: 4154
BsiEI CG_ry'CG
Cuts at: 104 1696 1742 2950 104
Size: 1592 46 1208 1308
Fragments arranged by size:
1592 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 630 946 1022 1168 1946 2136 2928 630
Size: 316 76 146 778 190 792 1856
Fragments arranged by size:
1856 792 778 316 190 146 76
BsmI GAATG_Cn'
Cuts at: 694 694
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 630 946 1011 1022 1168 1779 1872 1946 2136
Size: 316 65 11 146 611 93 74 190
Cuts at: 2136 2198 2928 630
Size: 62 730 1856
Fragments arranged by size:
1856 730 611 316 190 146 93 74
65 62 11
BspEI T'CCGG_A
Cuts at: 536 3374 536
Size: 2838 1316
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1204 1418 1720 2101 1204
Size: 214 302 381 3257
Fragments arranged by size:
3257 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1145 2066 1145
Size: 921 3233
BsrFI r'CCGG_y
Cuts at: 2152 2333 2542 2152
Size: 181 209 3764
Fragments arranged by size:
3764 209 181
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 1531
Size: 4154
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstEII G'GTnAC_C
Cuts at: 886 886
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 190 511 1004 190
Size: 321 493 3340
Fragments arranged by size:
3340 493 321
BstYI r'GATC_y
Cuts at: 1 1000 1179 1646 1669 2004 2250 3262 3348
Size: 999 179 467 23 335 246 1012 86
Cuts at: 3348 3360 3706 1
Size: 12 346 449
Fragments arranged by size:
1012 999 467 449 346 335 246 179
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 317 317
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 1397 1397
Size: 4154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 117 152 117
Size: 35 4119
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DraIII CAC_nnn'GTG
Cuts at: 301 301
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 527 1860 2718 527
Size: 1333 858 1963
Fragments arranged by size:
1963 1333 858
EaeI y'GGCC_r
Cuts at: 810 1739 1913 2304 2331 3676 810
Size: 929 174 391 27 1345 1288
Fragments arranged by size:
1345 1288 929 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 1121 2177 2387 1121
Size: 1056 210 2888
Fragments arranged by size:
2888 1056 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 1166 944
Size: 222 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 353 460 1036 1979 2411 3158 3426 343
Size: 10 107 576 943 432 747 268 1071
Fragments arranged by size:
1071 943 747 576 432 268 107 10
EcoNI CCTnn'n_nnAGG
Cuts at: 515 985 515
Size: 470 3684
EcoO109I rG'GnC_Cy
Cuts at: 195 321 337 586 1293 195
Size: 126 16 249 707 3056
Fragments arranged by size:
3056 707 249 126 16
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 1588 1836 2858 3837 1588
Size: 248 1022 979 1905
Fragments arranged by size:
1905 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 948 980 985 1017 1066 1098 1996 2026 2028
Size: 32 5 32 49 32 898 30 2
Cuts at: 2028 2058 948
Size: 30 3044
Fragments arranged by size:
3044 898 49 32 32 32 30 30
5 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
HindIII A'AGCT_T
Cuts at: 310 310
Size: 4154
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3009 2825
Size: 184 3970
MscI TGG'CCA
Cuts at: 812 1915 812
Size: 1103 3051
MslI CAynn'nnrTG
Cuts at: 931 1025 2143 2270 931
Size: 94 1118 127 2815
Fragments arranged by size:
2815 1118 127 94
MspA1I CmG'CkG
Cuts at: 551 1014 1265 1579 1939 2952 3197 551
Size: 463 251 314 360 1013 245 1508
Fragments arranged by size:
1508 1013 463 360 314 251 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PasI CC'CwG_GG
Cuts at: 190 190
Size: 4154
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PflMI CCAn_nnn'nTGG
Cuts at: 471 681 471
Size: 210 3944
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PpuMI rG'GwC_Cy
Cuts at: 195 195
Size: 4154
PspOMI G'GGCC_C
Cuts at: 1007 1007
Size: 4154
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 551 1014 1579 1939 551
Size: 463 565 360 2766
Fragments arranged by size:
2766 565 463 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 946 1168 946
Size: 222 3932
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SanDI GG'GwC_CC
Cuts at: 195 195
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 248 1197 1882 2875 3066 33
Size: 215 949 685 993 191 1121
Fragments arranged by size:
1121 993 949 685 215 191
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 217 1309 2224 2716 2978 217
Size: 1092 915 492 262 1393
Fragments arranged by size:
1393 1092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StyI C'CwwG_G
Cuts at: 582 1288 1642 582
Size: 706 354 3094
Fragments arranged by size:
3094 706 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 635 828 1737 1870 2329 2538 635
Size: 193 909 133 459 209 2251
Fragments arranged by size:
2251 909 459 209 193 133
TatI w'GTAC_w
Cuts at: 283 3669 283
Size: 3386 768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 620 651 652 683 3671 3703 620
Size: 31 1 31 2988 32 1071
Fragments arranged by size:
2988 1071 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstZ17I BtgI ClaI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI