Restriction map for
pF1KB4221
(Circular) (Six-base) MAPSORT of: pF1KB4221.seq Check: 4469 from: 1 to: 4796
pF1KB4221 4796 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 281 1834 281
Size: 1553 3243
Acc65I G'GTAC_C
Cuts at: 220 915 1812 220
Size: 695 897 3204
Fragments arranged by size:
3204 897 695
AclI AA'CG_TT
Cuts at: 383 4636 383
Size: 4253 543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 1207 1217 1291 2621 3053 3406 4068 137
Size: 1070 10 74 1330 432 353 662 865
Fragments arranged by size:
1330 1070 865 662 432 353 74 10
AflIII A'CryG_T
Cuts at: 2148 3948 4229 2148
Size: 1800 281 2715
Fragments arranged by size:
2715 1800 281
AleI CACnn'nnGTG
Cuts at: 1729 1729
Size: 4796
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1501 1533 1648 1680 1501
Size: 32 115 32 4617
Fragments arranged by size:
4617 115 32 32
AlwNI CAG_nnn'CTG
Cuts at: 165 237 1097 1285 1752 2101 3539 165
Size: 72 860 188 467 349 1438 1422
Fragments arranged by size:
1438 1422 860 467 349 188 72
ApaI G_GGCC'C
Cuts at: 777 799 777
Size: 22 4774
ApaLI G'TGCA_C
Cuts at: 3634 3634
Size: 4796
ApoI r'AATT_y
Cuts at: 1800 4149 4405 1800
Size: 2349 256 2191
Fragments arranged by size:
2349 2191 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 770 888 1130 1535 1816 770
Size: 118 242 405 281 3750
Fragments arranged by size:
3750 405 281 242 118
AvrII C'CTAG_G
Cuts at: 1786 1786
Size: 4796
BamHI G'GATC_C
Cuts at: 1821 1821
Size: 4796
BanI G'GyrC_C
Cuts at: 154 220 319 915 1458 1763 1812 2226 2474
Size: 66 99 596 543 305 49 414 248
Cuts at: 2474 2509 154
Size: 35 2441
Fragments arranged by size:
2441 596 543 414 305 248 99 66
49 35
BanII G_rGCy'C
Cuts at: 680 777 799 1028 1154 1547 1810 2840 680
Size: 97 22 229 126 393 263 1030 2636
Fragments arranged by size:
2636 1030 393 263 229 126 97 22
BbeI G_GCGC'C
Cuts at: 323 2230 2478 323
Size: 1907 248 2641
Fragments arranged by size:
2641 1907 248
BbsI GAAGACnn'nnnn_
Cuts at: 629 1470 629
Size: 841 3955
BbvCI CC'TCA_GC
Cuts at: 362 860 362
Size: 498 4298
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3750 4796 2694
Size: 1056 1046 2694
Fragments arranged by size:
2694 1056 1046
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BfrBI ATG'CAT
Cuts at: 126 126
Size: 4796
BglI GCCn_nnn'nGGC
Cuts at: 112 684 1075 112
Size: 572 391 3833
Fragments arranged by size:
3833 572 391
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 899 1908 899
Size: 1009 3787
Bme1580I G_kGCm'C
Cuts at: 159 737 777 799 889 1244 2421 2514 3638
Size: 578 40 22 90 355 1177 93 1124
Cuts at: 3638 159
Size: 1317
Fragments arranged by size:
1317 1177 1124 578 355 93 90 40
22
BmgBI CAC'GTC
Cuts at: 374 374
Size: 4796
BmrI ACTGGGnnnn_n'
Cuts at: 724 1790 2008 2089 2424 4568 724
Size: 1066 218 81 335 2144 952
Fragments arranged by size:
2144 1066 952 335 218 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 684 1674 3163 666
Size: 18 990 1489 2299
Fragments arranged by size:
2299 1489 990 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 679 1074 1330 1693 525
Size: 154 395 256 363 3628
Fragments arranged by size:
3628 395 363 256 154
Bpu10I CC'TnA_GC
Cuts at: 362 827 860 362
Size: 465 33 4298
Fragments arranged by size:
4298 465 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1093 1972 2851 3565 3863 709
Size: 384 879 879 714 298 1642
Fragments arranged by size:
1642 879 879 714 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 4500 361
Size: 4139 657
BsaAI yAC'GTr
Cuts at: 428 1731 2149 428
Size: 1303 418 3075
Fragments arranged by size:
3075 1303 418
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 320 2227 2475 3177 4673 320
Size: 1907 248 702 1496 443
Fragments arranged by size:
1907 1496 702 443 248
BsaWI w'CCGG_w
Cuts at: 980 1568 1985 2506 3184 3595 3742 4016 980
Size: 588 417 521 678 411 147 274 1760
Fragments arranged by size:
1760 678 588 521 417 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 962 992 358
Size: 30 574 30 4162
Fragments arranged by size:
4162 574 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 435 466 469 1566 379
Size: 56 31 3 1097 3609
Fragments arranged by size:
3609 1097 56 31 3
BseYI C'CCAG_C
Cuts at: 224 1015 1738 2221 2979 3644 4052 224
Size: 791 723 483 758 665 408 968
Fragments arranged by size:
968 791 758 723 665 483 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4796
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3614 104
Size: 2234 46 1230 1286
Fragments arranged by size:
2234 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 635 958 1718 1810 2588 2778 3638 635
Size: 323 760 92 778 190 860 1793
Fragments arranged by size:
1793 860 778 760 323 190 92
Bsp1286I G_dGCh'C
Cuts at: 159 635 680 737 777 799 889 958 1028
Size: 476 45 57 40 22 90 69 70
Cuts at: 1028 1154 1244 1547 1718 1810 2421 2514 2588
Size: 126 90 303 171 92 611 93 74
Cuts at: 2588 2778 2840 3638 159
Size: 190 62 798 1317
Fragments arranged by size:
1317 798 611 476 303 190 171 126
93 92 90 90 74 70 69 62
57 45 40 22
BspEI T'CCGG_A
Cuts at: 980 1568 980
Size: 588 4208
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 212 527 927 1846 2060 2362 2743 173
Size: 39 315 400 919 214 302 381 2226
Fragments arranged by size:
2226 919 400 381 315 302 214 39
BsrBI CCG'CTC
Cuts at: 50 579 3088 3142 4626 4791 50
Size: 529 2509 54 1484 165 55
Fragments arranged by size:
2509 1484 529 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2708 2708
Size: 4796
BsrFI r'CCGG_y
Cuts at: 711 2794 2975 711
Size: 2083 181 2532
Fragments arranged by size:
2532 2083 181
BssSI C'ACGA_G
Cuts at: 3067 3775 3067
Size: 708 4088
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstYI r'GATC_y
Cuts at: 1 1821 2288 2311 2646 2892 3198 3210 3296
Size: 1820 467 23 335 246 306 12 86
Cuts at: 3296 4348 1
Size: 1052 449
Fragments arranged by size:
1820 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1286 1286
Size: 4796
BtgI C'CryG_G
Cuts at: 107 611 1041 1247 1292 1490 107
Size: 504 430 206 45 198 3413
Fragments arranged by size:
3413 504 430 206 198 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1462 2039 1079
Size: 383 577 3836
Fragments arranged by size:
3836 577 383
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3846 2502
Size: 1344 3452
EaeI y'GGCC_r
Cuts at: 188 495 1038 1076 2381 2555 2946 2973 4318
Size: 307 543 38 1305 174 391 27 1345
Cuts at: 4318 188
Size: 666
Fragments arranged by size:
1345 1305 666 543 391 307 174 38
27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 113 1193 2819 3029 113
Size: 1080 1626 210 1880
Fragments arranged by size:
1880 1626 1080 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3004 3746 3892 4606 3004
Size: 742 146 714 3194
Fragments arranged by size:
3194 742 714 146
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 525 679 1074 1207 1217 1291 1330 1693
Size: 388 154 395 133 10 74 39 363
Cuts at: 1693 2621 3053 3406 4068 137
Size: 928 432 353 662 865
Fragments arranged by size:
928 865 662 432 395 388 363 353
154 133 74 39 10
EcoO109I rG'GnC_Cy
Cuts at: 795 796 876 1046 1203 1596 1683 1935 795
Size: 1 80 170 157 393 87 252 3656
Fragments arranged by size:
3656 393 252 170 157 87 80 1
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
EcoRV GAT'ATC
Cuts at: 591 591
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 771 2815 771
Size: 2044 2752
FspI TGC'GCA
Cuts at: 484 2577 484
Size: 2093 2703
HaeII r_GCGC'y
Cuts at: 323 2230 2478 3708 4479 323
Size: 1907 248 1230 771 640
Fragments arranged by size:
1907 1230 771 640 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 529 561 927 959 1603 1623 1635
Size: 32 139 32 366 32 644 20 12
Cuts at: 1635 1655 2638 2668 2670 2700 358
Size: 20 983 30 2 30 2454
Fragments arranged by size:
2454 983 644 366 139 32 32 32
30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1485 1835 1485
Size: 350 4446
KasI G'GCGC_C
Cuts at: 319 2226 2474 319
Size: 1907 248 2641
Fragments arranged by size:
2641 1907 248
KpnI G_GTAC'C
Cuts at: 224 919 1816 224
Size: 695 897 3204
Fragments arranged by size:
3204 897 695
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 3555 3739 401
Size: 3154 184 1458
Fragments arranged by size:
3154 1458 184
MscI TGG'CCA
Cuts at: 190 497 1040 1078 2557 190
Size: 307 543 38 1479 2429
Fragments arranged by size:
2429 1479 543 307 38
MslI CAynn'nnrTG
Cuts at: 638 1729 2785 2912 638
Size: 1091 1056 127 2522
Fragments arranged by size:
2522 1091 1056 127
MspA1I CmG'CkG
Cuts at: 228 234 1907 2221 2581 3365 3610 228
Size: 6 1673 314 360 784 245 1414
Fragments arranged by size:
1673 1414 784 360 314 245 6
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 320 2227 2475 320
Size: 1907 248 2641
Fragments arranged by size:
2641 1907 248
NcoI C'CATG_G
Cuts at: 107 1292 107
Size: 1185 3611
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NsiI A_TGCA'T
Cuts at: 128 128
Size: 4796
NspI r_CATG'y
Cuts at: 768 1849 2349 3952 768
Size: 1081 500 1603 1612
Fragments arranged by size:
1612 1603 1081 500
PasI CC'CwG_GG
Cuts at: 911 911
Size: 4796
PciI A'CATG_T
Cuts at: 3948 3948
Size: 4796
PflMI CCAn_nnn'nTGG
Cuts at: 847 847
Size: 4796
PfoI T'CCnGG_A
Cuts at: 998 998
Size: 4796
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PmlI CAC'GTG
Cuts at: 428 1731 428
Size: 1303 3493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 876 1203 1596 1683 876
Size: 327 393 87 3989
Fragments arranged by size:
3989 393 327 87
PspOMI G'GGCC_C
Cuts at: 773 795 773
Size: 22 4774
PstI C_TGCA'G
Cuts at: 233 1843 2528 233
Size: 1610 685 2501
Fragments arranged by size:
2501 1610 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 228 234 2221 2581 228
Size: 6 1987 360 2443
Fragments arranged by size:
2443 1987 360 6
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SanDI GG'GwC_CC
Cuts at: 876 876
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 229 1839 2524 3492 3683 33
Size: 196 1610 685 968 191 1146
Fragments arranged by size:
1610 1146 968 685 196 191
SfoI GGC'GCC
Cuts at: 321 2228 2476 321
Size: 1907 248 2641
Fragments arranged by size:
2641 1907 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 772 1537 1818 772
Size: 765 281 3750
Fragments arranged by size:
3750 765 281
SmlI C'TyrA_G
Cuts at: 688 1108 1951 2866 3580 3842 688
Size: 420 843 915 714 262 1642
Fragments arranged by size:
1642 915 843 714 420 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
StyI C'CwwG_G
Cuts at: 107 191 524 791 1210 1292 1786 1930 2284
Size: 84 333 267 419 82 494 144 354
Cuts at: 2284 107
Size: 2619
Fragments arranged by size:
2619 494 419 354 333 267 144 84
82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 722 1155 1464 1741 2379 2512 2971 3180 722
Size: 433 309 277 638 133 459 209 2338
Fragments arranged by size:
2338 638 459 433 309 277 209 133
TatI w'GTAC_w
Cuts at: 4311 4311
Size: 4796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 868 900 962 994 1223 1255 4313 4345 868
Size: 32 62 32 229 32 3058 32 1319
Fragments arranged by size:
3058 1319 229 62 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 372 2593 372
Size: 2221 2575
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 4796
XmaI C'CCGG_G
Cuts at: 770 1535 1816 770
Size: 765 281 3750
Fragments arranged by size:
3750 765 281
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AscI
BaeI BcgI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI