Restriction map for
pF1KB4231
(Circular) (Six-base) MAPSORT of: pF1KB4231.seq Check: 274 from: 1 to: 8660
pF1KB4231 8660 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 2997 3603 3740 5698 2997
Size: 606 137 1958 5959
Fragments arranged by size:
5959 1958 606 137
Acc65I G'GTAC_C
Cuts at: 5676 5676
Size: 8660
AclI AA'CG_TT
Cuts at: 8500 8500
Size: 8660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1016 1310 1684 2318 2726 3038 3271 3780
Size: 97 294 374 634 408 312 233 509
Cuts at: 3780 4247 5287 6485 6917 7664 7932 919
Size: 467 1040 1198 432 747 268 1647
Fragments arranged by size:
1647 1198 1040 747 634 509 467 432
408 374 312 294 268 233 97
AflII C'TTAA_G
Cuts at: 3816 3816
Size: 8660
AflIII A'CryG_T
Cuts at: 134 637 6012 7116 8093 134
Size: 503 5375 1104 977 701
Fragments arranged by size:
5375 1104 977 701 503
AgeI A'CCGG_T
Cuts at: 7048 7048
Size: 8660
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 3983 4015 2765
Size: 32 1186 32 7410
Fragments arranged by size:
7410 1186 32 32
AlwNI CAG_nnn'CTG
Cuts at: 952 1761 5965 7532 952
Size: 809 4204 1567 2080
Fragments arranged by size:
4204 2080 1567 809
ApaI G_GGCC'C
Cuts at: 529 529
Size: 8660
ApaLI G'TGCA_C
Cuts at: 7430 7430
Size: 8660
ApoI r'AATT_y
Cuts at: 324 602 1229 1320 1647 1694 2103 2377 2515
Size: 278 627 91 327 47 409 274 138
Cuts at: 2515 3231 3376 3665 4093 4648 4927 5664 8013
Size: 716 145 289 428 555 279 737 2349
Cuts at: 8013 8269 324
Size: 256 715
Fragments arranged by size:
2349 737 716 715 627 555 428 409
327 289 279 278 274 256 145 138
91 47
AseI AT'TA_AT
Cuts at: 20 484 1541 2506 20
Size: 464 1057 965 6174
Fragments arranged by size:
6174 1057 965 464
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8660
AvaI C'yCGr_G
Cuts at: 146 5680 146
Size: 5534 3126
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2082 2115 3005 3038 2082
Size: 33 890 33 7704
Fragments arranged by size:
7704 890 33 33
BamHI G'GATC_C
Cuts at: 5685 5685
Size: 8660
BanI G'GyrC_C
Cuts at: 2287 2907 5676 6090 6338 6373 2287
Size: 620 2769 414 248 35 4574
Fragments arranged by size:
4574 2769 620 414 248 35
BanII G_rGCy'C
Cuts at: 153 529 5674 6704 153
Size: 376 5145 1030 2109
Fragments arranged by size:
5145 2109 1030 376
BbeI G_GCGC'C
Cuts at: 6094 6342 6094
Size: 248 8412
BbsI GAAGACnn'nnnn_
Cuts at: 130 925 2467 2835 4235 5004 130
Size: 795 1542 368 1400 769 3786
Fragments arranged by size:
3786 1542 1400 795 769 368
BciVI GTATCCnnnnn_n'
Cuts at: 2565 4788 6558 7319 8660 2565
Size: 2223 1770 761 1341 2565
Fragments arranged by size:
2565 2223 1770 1341 761
BclI T'GATC_A
Cuts at: 870 1002 1734 3969 6180 870
Size: 132 732 2235 2211 3350
Fragments arranged by size:
3350 2235 2211 732 132
BfrBI ATG'CAT
Cuts at: 2401 3071 3100 5506 2401
Size: 670 29 2406 5555
Fragments arranged by size:
5555 2406 670 29
BglII A'GATC_T
Cuts at: 6175 8212 6175
Size: 2037 6623
BlpI GC'TnA_GC
Cuts at: 4556 5772 4556
Size: 1216 7444
Bme1580I G_kGCm'C
Cuts at: 440 529 4016 6285 6378 7434 440
Size: 89 3487 2269 93 1056 1666
Fragments arranged by size:
3487 2269 1666 1056 93 89
BmrI ACTGGGnnnn_n'
Cuts at: 5872 5953 6288 8432 5872
Size: 81 335 2144 6100
Fragments arranged by size:
6100 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 284 7027 284
Size: 6743 1917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 440 794 2131 3109 3314 4766 175
Size: 265 354 1337 978 205 1452 4069
Fragments arranged by size:
4069 1452 1337 978 354 265 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1862 2330 3768 3890 5087 5836 6715 7207 7505
Size: 468 1438 122 1197 749 879 492 298
Cuts at: 7505 1862
Size: 3017
Fragments arranged by size:
3017 1438 1197 879 749 492 468 298
122
BsaI GGTCTCn'nnnn_
Cuts at: 3665 3857 5269 8364 3665
Size: 192 1412 3095 3961
Fragments arranged by size:
3961 3095 1412 192
BsaAI yAC'GTr
Cuts at: 6013 6013
Size: 8660
BsaBI GATnn'nnATC
Cuts at: 6 6174 8211 6
Size: 6168 2037 455
Fragments arranged by size:
6168 2037 455
BsaHI Gr'CG_yC
Cuts at: 2968 6091 6339 7041 8537 2968
Size: 3123 248 702 1496 3091
Fragments arranged by size:
3123 3091 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3145 5312 5849 6370 7048 7322 7469 7880 3145
Size: 2167 537 521 678 274 147 411 3925
Fragments arranged by size:
3925 2167 678 537 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2249 2279 3392 3422 2249
Size: 30 1113 30 7487
Fragments arranged by size:
7487 1113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 257 5365 257
Size: 5108 3552
BseYI C'CCAG_C
Cuts at: 200 2740 5019 6085 6843 7420 7916 200
Size: 2540 2279 1066 758 577 496 944
Fragments arranged by size:
2540 2279 1066 944 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1187 1796 4594 4668 4996 998
Size: 189 609 2798 74 328 4662
Fragments arranged by size:
4662 2798 609 328 189 74
BsiEI CG_ry'CG
Cuts at: 104 2502 6202 6248 7456 104
Size: 2398 3700 46 1208 1308
Fragments arranged by size:
3700 2398 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 5674 6452 6642 7434 5674
Size: 778 190 792 6900
Fragments arranged by size:
6900 792 778 190
BsmI GAATG_Cn'
Cuts at: 1509 1954 2542 2983 3080 3997 5303 1509
Size: 445 588 441 97 917 1306 4866
Fragments arranged by size:
4866 1306 917 588 445 441 97
Bsp1286I G_dGCh'C
Cuts at: 153 440 529 4016 5674 6285 6378 6452 6642
Size: 287 89 3487 1658 611 93 74 190
Cuts at: 6642 6704 7434 153
Size: 62 730 1379
Fragments arranged by size:
3487 1658 1379 730 611 287 190 93
89 74 62
BspEI T'CCGG_A
Cuts at: 5312 7880 5312
Size: 2568 6092
BspHI T'CATG_A
Cuts at: 606 1449 4824 7836 606
Size: 843 3375 3012 1430
Fragments arranged by size:
3375 3012 1430 843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5710 5924 6226 6607 5710
Size: 214 302 381 7763
Fragments arranged by size:
7763 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6952 7006 8490 8655 50
Size: 6902 54 1484 165 55
Fragments arranged by size:
6902 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 456 1474 4110 6572 456
Size: 1018 2636 2462 2544
Fragments arranged by size:
2636 2544 2462 1018
BsrFI r'CCGG_y
Cuts at: 6658 6839 7048 6658
Size: 181 209 8270
Fragments arranged by size:
8270 209 181
BsrGI T'GTAC_A
Cuts at: 1092 5318 1092
Size: 4226 4434
BssSI C'ACGA_G
Cuts at: 823 6931 7289 823
Size: 6108 358 2194
Fragments arranged by size:
6108 2194 358
BstAPI GCAn_nnn'nTGC
Cuts at: 215 4486 6037 215
Size: 4271 1551 2838
Fragments arranged by size:
4271 2838 1551
BstBI TT'CG_AA
Cuts at: 7021 7021
Size: 8660
BstXI CCAn_nnnn'nTGG
Cuts at: 5286 5286
Size: 8660
BstYI r'GATC_y
Cuts at: 1 961 1437 3340 3499 5685 6152 6175 6510
Size: 960 476 1903 159 2186 467 23 335
Cuts at: 6510 6756 7768 7854 7866 8212 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2186 1903 1012 960 476 467 449 346
335 246 159 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1930 4181 1930
Size: 2251 6409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4220 6610 6791 6954 4220
Size: 2390 181 163 5926
Fragments arranged by size:
5926 2390 181 163
BtsI GCAGTG_nn'
Cuts at: 4082 5388 5903 4082
Size: 1306 515 6839
Fragments arranged by size:
6839 1306 515
ClaI AT'CG_AT
Cuts at: 2498 2502 2498
Size: 4 8656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4829 4864 4829
Size: 35 8625
DraI TTT'AAA
Cuts at: 1689 2102 5496 5659 1689
Size: 413 3394 163 4690
Fragments arranged by size:
4690 3394 413 163
DraIII CAC_nnn'GTG
Cuts at: 1342 1342
Size: 8660
DrdI GACnn_nn'nnGTC
Cuts at: 5008 6366 7224 5008
Size: 1358 858 6444
Fragments arranged by size:
6444 1358 858
EaeI y'GGCC_r
Cuts at: 167 2861 4353 6245 6419 6810 6837 8182 167
Size: 2694 1492 1892 174 391 27 1345 645
Fragments arranged by size:
2694 1892 1492 1345 645 391 174 27
EagI C'GGCC_G
Cuts at: 6245 6245
Size: 8660
EarI CTCTTCn'nnn_
Cuts at: 1299 2702 5314 6683 6893 1299
Size: 1403 2612 1369 210 3066
Fragments arranged by size:
3066 2612 1403 1369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6868 7178 7324 8470 6868
Size: 310 146 1146 7058
Fragments arranged by size:
7058 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5672 5672
Size: 8660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 440 794 919 1016 1310 1684 2131 2318
Size: 265 354 125 97 294 374 447 187
Cuts at: 2318 2726 3038 3109 3271 3314 3780 4247 4766
Size: 408 312 71 162 43 466 467 519
Cuts at: 4766 5287 6485 6917 7664 7932 175
Size: 521 1198 432 747 268 903
Fragments arranged by size:
1198 903 747 521 519 467 466 447
432 408 374 354 312 294 268 265
187 162 125 97 71 43
EcoO109I rG'GnC_Cy
Cuts at: 525 526 897 1330 1510 2187 5799 525
Size: 1 371 433 180 677 3612 3386
Fragments arranged by size:
3612 3386 677 433 371 180 1
EcoRI G'AATT_C
Cuts at: 1229 5664 1229
Size: 4435 4225
EcoRV GAT'ATC
Cuts at: 2753 4695 5615 2753
Size: 1942 920 5798
Fragments arranged by size:
5798 1942 920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 366 916 1392 2299 3361 3802 3831 6679 366
Size: 550 476 907 1062 441 29 2848 2347
Fragments arranged by size:
2848 2347 1062 907 550 476 441 29
FspI TGC'GCA
Cuts at: 208 5302 6441 208
Size: 5094 1139 2427
Fragments arranged by size:
5094 2427 1139
HaeII r_GCGC'y
Cuts at: 6094 6342 7364 8343 6094
Size: 248 1022 979 6411
Fragments arranged by size:
6411 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 817 849 855 862 887 894 1270
Size: 32 650 32 6 7 25 7 376
Cuts at: 1270 1274 1302 1306 1429 1461 1977 2009 2104
Size: 4 28 4 123 32 516 32 95
Cuts at: 2104 2136 2540 2572 2701 2733 3041 3073 4786
Size: 32 404 32 129 32 308 32 1713
Cuts at: 4786 4818 6502 6532 6534 6564 135
Size: 32 1684 30 2 30 2231
Fragments arranged by size:
2231 1713 1684 650 516 404 376 308
129 123 95 32 32 32 32 32
32 32 32 32 30 30 28 25
7 7 6 4 4 2
HincII GTy'rAC
Cuts at: 2117 2609 4727 5699 2117
Size: 492 2118 972 5078
Fragments arranged by size:
5078 2118 972 492
HindIII A'AGCT_T
Cuts at: 212 212
Size: 8660
KasI G'GCGC_C
Cuts at: 6090 6338 6090
Size: 248 8412
KpnI G_GTAC'C
Cuts at: 5680 5680
Size: 8660
MluI A'CGCG_T
Cuts at: 8093 8093
Size: 8660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1110 3248 3378 3880 5225 7331 7515 1110
Size: 2138 130 502 1345 2106 184 2255
Fragments arranged by size:
2255 2138 2106 1345 502 184 130
MscI TGG'CCA
Cuts at: 2863 4355 6421 2863
Size: 1492 2066 5102
Fragments arranged by size:
5102 2066 1492
MslI CAynn'nnrTG
Cuts at: 706 914 1067 2668 3212 4126 4361 6649 6776
Size: 208 153 1601 544 914 235 2288 127
Cuts at: 6776 706
Size: 2590
Fragments arranged by size:
2590 2288 1601 914 544 235 208 153
127
MspA1I CmG'CkG
Cuts at: 204 552 1018 3087 4980 5771 6085 6445 7458
Size: 348 466 2069 1893 791 314 360 1013
Cuts at: 7458 7703 204
Size: 245 1161
Fragments arranged by size:
2069 1893 1161 1013 791 466 360 348
314 245
NaeI GCC'GGC
Cuts at: 6841 6841
Size: 8660
NarI GG'CG_CC
Cuts at: 6091 6339 6091
Size: 248 8412
NdeI CA'TA_TG
Cuts at: 2403 3102 4350 2403
Size: 699 1248 6713
Fragments arranged by size:
6713 1248 699
NgoMIV G'CCGG_C
Cuts at: 6839 6839
Size: 8660
NsiI A_TGCA'T
Cuts at: 2403 3073 3102 5508 2403
Size: 670 29 2406 5555
Fragments arranged by size:
5555 2406 670 29
NspI r_CATG'y
Cuts at: 138 371 641 2941 3071 4720 5713 6213 7120
Size: 233 270 2300 130 1649 993 500 907
Cuts at: 7120 138
Size: 1678
Fragments arranged by size:
2300 1678 1649 993 907 500 270 233
130
PciI A'CATG_T
Cuts at: 134 637 7116 134
Size: 503 6479 1678
Fragments arranged by size:
6479 1678 503
PfoI T'CCnGG_A
Cuts at: 1233 4067 1233
Size: 2834 5826
PmeI GTTT'AAAC
Cuts at: 5659 5659
Size: 8660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3928 3960 7799 7831 3928
Size: 32 3839 32 4757
Fragments arranged by size:
4757 3839 32 32
PpuMI rG'GwC_Cy
Cuts at: 897 1330 1510 2187 897
Size: 433 180 677 7370
Fragments arranged by size:
7370 677 433 180
PspOMI G'GGCC_C
Cuts at: 525 525
Size: 8660
PstI C_TGCA'G
Cuts at: 557 582 1017 1502 5061 5383 5707 6392 557
Size: 25 435 485 3559 322 324 685 2825
Fragments arranged by size:
3559 2825 685 485 435 324 322 25
PvuI CG_AT'CG
Cuts at: 104 2502 104
Size: 2398 6262
PvuII CAG'CTG
Cuts at: 204 552 1018 3087 4980 6085 6445 204
Size: 348 466 2069 1893 1105 360 2419
Fragments arranged by size:
2419 2069 1893 1105 466 360 348
RsrII CG'GwC_CG
Cuts at: 6855 6855
Size: 8660
SacI G_AGCT'C
Cuts at: 5674 5674
Size: 8660
SalI G'TCGA_C
Cuts at: 5697 5697
Size: 8660
SapI GCTCTTCn'nnn_
Cuts at: 6683 6893 6683
Size: 210 8450
SbfI CC_TGCA'GG
Cuts at: 5707 5707
Size: 8660
ScaI AGT'ACT
Cuts at: 346 4523 8177 346
Size: 4177 3654 829
Fragments arranged by size:
4177 3654 829
SexAI A'CCwGG_T
Cuts at: 1338 1338
Size: 8660
SfcI C'TryA_G
Cuts at: 33 553 578 1013 1187 1498 2142 3479 4814
Size: 520 25 435 174 311 644 1337 1335
Cuts at: 4814 4919 5057 5379 5703 6388 7381 7572 33
Size: 105 138 322 324 685 993 191 1121
Fragments arranged by size:
1337 1335 1121 993 685 644 520 435
324 322 311 191 174 138 105 25
SfoI GGC'GCC
Cuts at: 6092 6340 6092
Size: 248 8412
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8660
SmaI CCC'GGG
Cuts at: 148 5682 148
Size: 5534 3126
SmlI C'TyrA_G
Cuts at: 1877 2345 3747 3816 3905 5102 5815 6730 7222
Size: 468 1402 69 89 1197 713 915 492
Cuts at: 7222 7484 1877
Size: 262 3053
Fragments arranged by size:
3053 1402 1197 915 713 492 468 262
89 69
SpeI A'CTAG_T
Cuts at: 67 1851 67
Size: 1784 6876
SphI G_CATG'C
Cuts at: 371 3071 5713 6213 371
Size: 2700 2642 500 2818
Fragments arranged by size:
2818 2700 2642 500
SspI AAT'ATT
Cuts at: 1215 3491 1215
Size: 2276 6384
StyI C'CwwG_G
Cuts at: 2544 4840 5109 5794 6148 2544
Size: 2296 269 685 354 5056
Fragments arranged by size:
5056 2296 685 354 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2341 3602 3692 4230 6243 6376 6835 7044 2341
Size: 1261 90 538 2013 133 459 209 3957
Fragments arranged by size:
3957 2013 1261 538 459 209 133 90
TatI w'GTAC_w
Cuts at: 344 397 463 618 1092 2146 4521 4533 5318
Size: 53 66 155 474 1054 2375 12 785
Cuts at: 5318 8175 344
Size: 2857 829
Fragments arranged by size:
2857 2375 1054 829 785 474 155 66
53 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 3281 3313 3392 3424 8177 8209 2154
Size: 32 1095 32 79 32 4753 32 2605
Fragments arranged by size:
4753 2605 1095 79 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6457 6457
Size: 8660
XbaI T'CTAG_A
Cuts at: 405 2460 5691 405
Size: 2055 3231 3374
Fragments arranged by size:
3374 3231 2055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2023 2955 6155 2023
Size: 932 3200 4528
Fragments arranged by size:
4528 3200 932
XmaI C'CCGG_G
Cuts at: 146 5680 146
Size: 5534 3126
XmnI GAAnn'nnTTC
Cuts at: 136 545 1214 1323 2468 3811 3940 4136 136
Size: 409 669 109 1145 1343 129 196 4660
Fragments arranged by size:
4660 1343 1145 669 409 196 129 109
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I BtgI EcoNI FseI FspAI HpaI
MfeI NcoI NheI NotI NruI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI