Restriction map for
pF1KB4239
(Circular) (Six-base) MAPSORT of: pF1KB4239.seq Check: 7288 from: 1 to: 6737
pF1KB4239 6737 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 2631 2631
Size: 6737
AccI GT'mk_AC
Cuts at: 647 3775 647
Size: 3128 3609
Acc65I G'GTAC_C
Cuts at: 3753 3753
Size: 6737
AclI AA'CG_TT
Cuts at: 6577 6577
Size: 6737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 920 1190 3615 4562 4994 5741 6009 179
Size: 741 270 2425 947 432 747 268 907
Fragments arranged by size:
2425 947 907 747 741 432 270 268
AflIII A'CryG_T
Cuts at: 4089 5193 6170 4089
Size: 1104 977 4656
Fragments arranged by size:
4656 1104 977
AgeI A'CCGG_T
Cuts at: 5125 5125
Size: 6737
AhdI GACnn_n'nnGTC
Cuts at: 2499 2499
Size: 6737
AlwNI CAG_nnn'CTG
Cuts at: 4042 5609 4042
Size: 1567 5170
ApaLI G'TGCA_C
Cuts at: 5507 5507
Size: 6737
ApoI r'AATT_y
Cuts at: 166 478 502 789 2050 2767 3076 3479 3741
Size: 312 24 287 1261 717 309 403 262
Cuts at: 3741 6090 6346 166
Size: 2349 256 557
Fragments arranged by size:
2349 1261 717 557 403 312 309 287
262 256 24
AseI AT'TA_AT
Cuts at: 20 1790 20
Size: 1770 4967
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6737
AvaI C'yCGr_G
Cuts at: 3757 3757
Size: 6737
BamHI G'GATC_C
Cuts at: 386 1944 3762 386
Size: 1558 1818 3361
Fragments arranged by size:
3361 1818 1558
BanI G'GyrC_C
Cuts at: 3041 3655 3753 4167 4415 4450 3041
Size: 614 98 414 248 35 5328
Fragments arranged by size:
5328 614 414 248 98 35
BanII G_rGCy'C
Cuts at: 2376 3751 4781 2376
Size: 1375 1030 4332
Fragments arranged by size:
4332 1375 1030
BbeI G_GCGC'C
Cuts at: 4171 4419 4171
Size: 248 6489
BbsI GAAGACnn'nnnn_
Cuts at: 842 1242 2531 842
Size: 400 1289 5048
Fragments arranged by size:
5048 1289 400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1432 1466 1552 1586 1432
Size: 34 86 34 6583
Fragments arranged by size:
6583 86 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1693 4635 5396 6737 1693
Size: 2942 761 1341 1693
Fragments arranged by size:
2942 1693 1341 761
BclI T'GATC_A
Cuts at: 4257 4257
Size: 6737
BglI GCCn_nnn'nGGC
Cuts at: 3039 3039
Size: 6737
BglII A'GATC_T
Cuts at: 666 4252 6289 666
Size: 3586 2037 1114
Fragments arranged by size:
3586 2037 1114
BlpI GC'TnA_GC
Cuts at: 3849 3849
Size: 6737
Bme1580I G_kGCm'C
Cuts at: 404 4362 4455 5511 404
Size: 3958 93 1056 1630
Fragments arranged by size:
3958 1630 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 460 1210 2371 3199 3949 4030 4365 6509 460
Size: 750 1161 828 750 81 335 2144 688
Fragments arranged by size:
2144 1161 828 750 750 688 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1023 3150 5104 1023
Size: 2127 1954 2656
Fragments arranged by size:
2656 2127 1954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 1782 2030 2222 2528 2540 1525
Size: 257 248 192 306 12 5722
Fragments arranged by size:
5722 306 257 248 192 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1904 3105 3329 3913 4792 5284 5582 907
Size: 997 1201 224 584 879 492 298 2062
Fragments arranged by size:
2062 1201 997 879 584 492 298 224
BsaI GGTCTCn'nnnn_
Cuts at: 3611 3631 6441 3611
Size: 20 2810 3907
Fragments arranged by size:
3907 2810 20
BsaAI yAC'GTr
Cuts at: 4090 4090
Size: 6737
BsaBI GATnn'nnATC
Cuts at: 6 2246 3464 4251 6288 6
Size: 2240 1218 787 2037 455
Fragments arranged by size:
2240 2037 1218 787 455
BsaHI Gr'CG_yC
Cuts at: 2428 2628 4168 4416 5118 6614 2428
Size: 200 1540 248 702 1496 2551
Fragments arranged by size:
2551 1540 1496 702 248 200
BsaWI w'CCGG_w
Cuts at: 1138 1941 3926 4447 5125 5399 5546 5957 1138
Size: 803 1985 521 678 274 147 411 1918
Fragments arranged by size:
1985 1918 803 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2363 2393 3144 3174 2363
Size: 30 751 30 5926
Fragments arranged by size:
5926 751 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 553 1088 553
Size: 535 6202
BseYI C'CCAG_C
Cuts at: 1573 3034 4162 4920 5497 5993 1573
Size: 1461 1128 758 577 496 2317
Fragments arranged by size:
2317 1461 1128 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1020 1020
Size: 6737
BsiEI CG_ry'CG
Cuts at: 104 4279 4325 5533 104
Size: 4175 46 1208 1308
Fragments arranged by size:
4175 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3751 4529 4719 5511 3751
Size: 778 190 792 4977
Fragments arranged by size:
4977 792 778 190
BsmI GAATG_Cn'
Cuts at: 3063 3063
Size: 6737
Bsp1286I G_dGCh'C
Cuts at: 404 2376 3751 4362 4455 4529 4719 4781 5511
Size: 1972 1375 611 93 74 190 62 730
Cuts at: 5511 404
Size: 1630
Fragments arranged by size:
1972 1630 1375 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1941 5957 1941
Size: 4016 2721
BspHI T'CATG_A
Cuts at: 2217 5913 2217
Size: 3696 3041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3787 4001 4303 4684 3787
Size: 214 302 381 5840
Fragments arranged by size:
5840 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5029 5083 6567 6732 50
Size: 4979 54 1484 165 55
Fragments arranged by size:
4979 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 711 1152 1437 1540 2413 3702 4649 711
Size: 441 285 103 873 1289 947 2799
Fragments arranged by size:
2799 1289 947 873 441 285 103
BsrFI r'CCGG_y
Cuts at: 4735 4916 5125 4735
Size: 181 209 6347
Fragments arranged by size:
6347 209 181
BsrGI T'GTAC_A
Cuts at: 750 750
Size: 6737
BssSI C'ACGA_G
Cuts at: 5008 5366 5008
Size: 358 6379
BstAPI GCAn_nnn'nTGC
Cuts at: 4114 4114
Size: 6737
BstBI TT'CG_AA
Cuts at: 5098 5098
Size: 6737
BstXI CCAn_nnnn'nTGG
Cuts at: 2384 2384
Size: 6737
BstYI r'GATC_y
Cuts at: 1 386 546 666 1944 2482 3667 3682 3762
Size: 385 160 120 1278 538 1185 15 80
Cuts at: 3762 4229 4252 4587 4833 5845 5931 5943 6289
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6289 1
Size: 449
Fragments arranged by size:
1278 1185 1012 538 467 449 385 346
335 246 160 120 86 80 23 15
12
BstZ17I GTA'TAC
Cuts at: 648 648
Size: 6737
Bsu36I CC'TnA_GG
Cuts at: 1460 3141 1460
Size: 1681 5056
BtgI C'CryG_G
Cuts at: 107 1326 2318 2553 3621 107
Size: 1219 992 235 1068 3223
Fragments arranged by size:
3223 1219 1068 992 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4687 4868 5031 4687
Size: 181 163 6393
Fragments arranged by size:
6393 181 163
BtsI GCAGTG_nn'
Cuts at: 849 2897 3980 849
Size: 2048 1083 3606
Fragments arranged by size:
3606 2048 1083
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 921 956 921
Size: 35 6702
DraI TTT'AAA
Cuts at: 3736 3736
Size: 6737
DrdI GACnn_nn'nnGTC
Cuts at: 3598 4443 5301 3598
Size: 845 858 5034
Fragments arranged by size:
5034 858 845
EaeI y'GGCC_r
Cuts at: 4322 4496 4887 4914 6259 4322
Size: 174 391 27 1345 4800
Fragments arranged by size:
4800 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4322 4322
Size: 6737
EarI CTCTTCn'nnn_
Cuts at: 123 1085 2063 3386 3412 3634 4760 4970 123
Size: 962 978 1323 26 222 1126 210 1890
Fragments arranged by size:
1890 1323 1126 978 962 222 210 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4945 5255 5401 6547 4945
Size: 310 146 1146 5135
Fragments arranged by size:
5135 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3749 3749
Size: 6737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 920 1190 1525 1782 2030 2222 2528 2540
Size: 741 270 335 257 248 192 306 12
Cuts at: 2540 3615 4562 4994 5741 6009 179
Size: 1075 947 432 747 268 907
Fragments arranged by size:
1075 947 907 747 741 432 335 306
270 268 257 248 192 12
EcoNI CCTnn'n_nnAGG
Cuts at: 575 3539 575
Size: 2964 3773
EcoO109I rG'GnC_Cy
Cuts at: 3876 3876
Size: 6737
EcoRI G'AATT_C
Cuts at: 3741 3741
Size: 6737
EcoRV GAT'ATC
Cuts at: 3173 3173
Size: 6737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2840 3565 4756 2840
Size: 725 1191 4821
Fragments arranged by size:
4821 1191 725
FspI TGC'GCA
Cuts at: 4518 4518
Size: 6737
HaeII r_GCGC'y
Cuts at: 4171 4419 5441 6420 4171
Size: 248 1022 979 4488
Fragments arranged by size:
4488 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 122 154 1561 1593 2485 2517 3322 3354 3667
Size: 32 1407 32 892 32 805 32 313
Cuts at: 3667 3699 4579 4609 4611 4641 122
Size: 32 880 30 2 30 2218
Fragments arranged by size:
2218 1407 892 880 805 313 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 780 1221 2600 3776 780
Size: 441 1379 1176 3741
Fragments arranged by size:
3741 1379 1176 441
HpaI GTT'AAC
Cuts at: 780 780
Size: 6737
KasI G'GCGC_C
Cuts at: 4167 4415 4167
Size: 248 6489
KpnI G_GTAC'C
Cuts at: 3757 3757
Size: 6737
MfeI C'AATT_G
Cuts at: 179 551 179
Size: 372 6365
MluI A'CGCG_T
Cuts at: 6170 6170
Size: 6737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 235 2654 5408 5592 235
Size: 2419 2754 184 1380
Fragments arranged by size:
2754 2419 1380 184
MscI TGG'CCA
Cuts at: 4498 4498
Size: 6737
MslI CAynn'nnrTG
Cuts at: 309 1526 2170 4726 4853 309
Size: 1217 644 2556 127 2193
Fragments arranged by size:
2556 2193 1217 644 127
MspA1I CmG'CkG
Cuts at: 431 3038 3848 4162 4522 5535 5780 431
Size: 2607 810 314 360 1013 245 1388
Fragments arranged by size:
2607 1388 1013 810 360 314 245
NaeI GCC'GGC
Cuts at: 4918 4918
Size: 6737
NarI GG'CG_CC
Cuts at: 4168 4416 4168
Size: 248 6489
NcoI C'CATG_G
Cuts at: 107 1326 2318 2553 3621 107
Size: 1219 992 235 1068 3223
Fragments arranged by size:
3223 1219 1068 992 235
NdeI CA'TA_TG
Cuts at: 1697 1697
Size: 6737
NgoMIV G'CCGG_C
Cuts at: 4916 4916
Size: 6737
NspI r_CATG'y
Cuts at: 3790 4290 5197 3790
Size: 500 907 5330
Fragments arranged by size:
5330 907 500
PasI CC'CwG_GG
Cuts at: 1256 1256
Size: 6737
PciI A'CATG_T
Cuts at: 5193 5193
Size: 6737
PflMI CCAn_nnn'nTGG
Cuts at: 1237 2559 1237
Size: 1322 5415
PfoI T'CCnGG_A
Cuts at: 1759 1759
Size: 6737
PmeI GTTT'AAAC
Cuts at: 3736 3736
Size: 6737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1050 1082 5876 5908 1050
Size: 32 4794 32 1879
Fragments arranged by size:
4794 1879 32 32
PshAI GACnn'nnGTC
Cuts at: 2098 3623 2098
Size: 1525 5212
PsiI TTA'TAA
Cuts at: 2296 2569 2296
Size: 273 6464
PstI C_TGCA'G
Cuts at: 1620 3784 4469 1620
Size: 2164 685 3888
Fragments arranged by size:
3888 2164 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6737
PvuII CAG'CTG
Cuts at: 3038 4162 4522 3038
Size: 1124 360 5253
Fragments arranged by size:
5253 1124 360
RsrII CG'GwC_CG
Cuts at: 4932 4932
Size: 6737
SacI G_AGCT'C
Cuts at: 3751 3751
Size: 6737
SalI G'TCGA_C
Cuts at: 3774 3774
Size: 6737
SapI GCTCTTCn'nnn_
Cuts at: 1085 2063 3386 4760 4970 1085
Size: 978 1323 1374 210 2852
Fragments arranged by size:
2852 1374 1323 978 210
SbfI CC_TGCA'GG
Cuts at: 3784 3784
Size: 6737
ScaI AGT'ACT
Cuts at: 3439 6254 3439
Size: 2815 3922
SexAI A'CCwGG_T
Cuts at: 2593 2593
Size: 6737
SfcI C'TryA_G
Cuts at: 33 1409 1604 1616 3707 3780 4465 5458 5649
Size: 1376 195 12 2091 73 685 993 191
Cuts at: 5649 33
Size: 1121
Fragments arranged by size:
2091 1376 1121 993 685 195 191 73
12
SfoI GGC'GCC
Cuts at: 4169 4417 4169
Size: 248 6489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6737
SmaI CCC'GGG
Cuts at: 3759 3759
Size: 6737
SmlI C'TyrA_G
Cuts at: 886 1919 3084 3344 3892 4807 5299 5561 886
Size: 1033 1165 260 548 915 492 262 2062
Fragments arranged by size:
2062 1165 1033 915 548 492 262 260
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6737
SphI G_CATG'C
Cuts at: 3790 4290 3790
Size: 500 6237
SspI AAT'ATT
Cuts at: 612 1353 2031 612
Size: 741 678 5318
Fragments arranged by size:
5318 741 678
StuI AGG'CCT
Cuts at: 581 2339 581
Size: 1758 4979
StyI C'CwwG_G
Cuts at: 107 1326 1863 2103 2282 2318 2553 3621 3871
Size: 1219 537 240 179 36 235 1068 250
Cuts at: 3871 4225 107
Size: 354 2619
Fragments arranged by size:
2619 1219 1068 537 354 250 240 235
179 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3218 3542 4320 4453 4912 5121 3218
Size: 324 778 133 459 209 4834
Fragments arranged by size:
4834 778 459 324 209 133
TatI w'GTAC_w
Cuts at: 750 2345 2575 2619 3071 3437 6252 750
Size: 1595 230 44 452 366 2815 1235
Fragments arranged by size:
2815 1595 1235 452 366 230 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 3414 3446 6254 6286 2363
Size: 32 1019 32 2808 32 2814
Fragments arranged by size:
2814 2808 1019 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4534 4534
Size: 6737
XbaI T'CTAG_A
Cuts at: 1896 3768 1896
Size: 1872 4865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 308 923 1050 4232 308
Size: 615 127 3182 2813
Fragments arranged by size:
3182 2813 615 127
XmaI C'CCGG_G
Cuts at: 3757 3757
Size: 6737
XmnI GAAnn'nnTTC
Cuts at: 1958 1958
Size: 6737
ZraI GAC'GTC
Cuts at: 2629 2629
Size: 6737
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII ClaI DraIII FseI FspAI HindIII
NheI NotI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI