Restriction map for
pF1KB4240
(Circular) (Six-base) MAPSORT of: pF1KB4240.seq Check: 895 from: 1 to: 4526
pF1KB4240 4526 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 537 556 537
Size: 19 4507
AccI GT'mk_AC
Cuts at: 832 1564 832
Size: 732 3794
Acc65I G'GTAC_C
Cuts at: 805 1542 805
Size: 737 3789
AclI AA'CG_TT
Cuts at: 4366 4366
Size: 4526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 737 1069 1192 1314 2351 2783 3530 3798
Size: 228 332 123 122 1037 432 747 268
Cuts at: 3798 509
Size: 1237
Fragments arranged by size:
1237 1037 747 432 332 268 228 123
122
AfeI AGC'GCT
Cuts at: 652 652
Size: 4526
AflIII A'CryG_T
Cuts at: 1203 1878 2982 3959 1203
Size: 675 1104 977 1770
Fragments arranged by size:
1770 1104 977 675
AgeI A'CCGG_T
Cuts at: 2914 2914
Size: 4526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1468 1468
Size: 4526
AlwNI CAG_nnn'CTG
Cuts at: 1831 3398 1831
Size: 1567 2959
ApaLI G'TGCA_C
Cuts at: 3296 3296
Size: 4526
ApoI r'AATT_y
Cuts at: 720 1530 3879 4135 720
Size: 810 2349 256 1111
Fragments arranged by size:
2349 1111 810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
AvaI C'yCGr_G
Cuts at: 1546 1546
Size: 4526
BamHI G'GATC_C
Cuts at: 1551 1551
Size: 4526
BanI G'GyrC_C
Cuts at: 138 274 301 805 850 951 1542 1956 2204
Size: 136 27 504 45 101 591 414 248
Cuts at: 2204 2239 138
Size: 35 2425
Fragments arranged by size:
2425 591 504 414 248 136 101 45
35 27
BanII G_rGCy'C
Cuts at: 643 1540 2570 643
Size: 897 1030 2599
Fragments arranged by size:
2599 1030 897
BbeI G_GCGC'C
Cuts at: 1960 2208 1960
Size: 248 4278
BbvCI CC'TCA_GC
Cuts at: 153 348 407 1466 1484 153
Size: 195 59 1059 18 3195
Fragments arranged by size:
3195 1059 195 59 18
BciVI GTATCCnnnnn_n'
Cuts at: 2424 3185 4526 2424
Size: 761 1341 2424
Fragments arranged by size:
2424 1341 761
BclI T'GATC_A
Cuts at: 2046 2046
Size: 4526
BglI GCCn_nnn'nGGC
Cuts at: 1480 1480
Size: 4526
BglII A'GATC_T
Cuts at: 2041 4078 2041
Size: 2037 2489
BlpI GC'TnA_GC
Cuts at: 1508 1638 1508
Size: 130 4396
Bme1580I G_kGCm'C
Cuts at: 304 2151 2244 3300 304
Size: 1847 93 1056 1530
Fragments arranged by size:
1847 1530 1056 93
BmgBI CAC'GTC
Cuts at: 1204 1204
Size: 4526
BmrI ACTGGGnnnn_n'
Cuts at: 584 1738 1819 2154 4298 584
Size: 1154 81 335 2144 812
Fragments arranged by size:
2144 1154 812 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 363 2893 363
Size: 2530 1996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1310
Size: 4526
Bpu10I CC'TnA_GC
Cuts at: 153 348 407 1141 1466 1484 153
Size: 195 59 734 325 18 3195
Fragments arranged by size:
3195 734 325 195 59 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1702 2581 3073 3371 1702
Size: 879 492 298 2857
Fragments arranged by size:
2857 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4230 4230
Size: 4526
BsaAI yAC'GTr
Cuts at: 982 1879 982
Size: 897 3629
BsaBI GATnn'nnATC
Cuts at: 6 2040 4077 6
Size: 2034 2037 455
Fragments arranged by size:
2037 2034 455
BsaHI Gr'CG_yC
Cuts at: 1957 2205 2907 4403 1957
Size: 248 702 1496 2080
Fragments arranged by size:
2080 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1715 2236 2914 3188 3335 3746 1715
Size: 521 678 274 147 411 2495
Fragments arranged by size:
2495 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 677 977 1422 1454 385
Size: 292 300 445 32 3457
Fragments arranged by size:
3457 445 300 292 32
BseYI C'CCAG_C
Cuts at: 1951 2709 3286 3782 1951
Size: 758 577 496 2695
Fragments arranged by size:
2695 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1484 161
Size: 1323 3203
BsiEI CG_ry'CG
Cuts at: 104 2068 2114 3322 104
Size: 1964 46 1208 1308
Fragments arranged by size:
1964 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1540 2318 2508 3300 1540
Size: 778 190 792 2766
Fragments arranged by size:
2766 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 304 643 1540 2151 2244 2318 2508 2570 3300
Size: 339 897 611 93 74 190 62 730
Cuts at: 3300 304
Size: 1530
Fragments arranged by size:
1530 897 730 611 339 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3746 3746
Size: 4526
BspHI T'CATG_A
Cuts at: 3702 3702
Size: 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 556 1101 1460 1576 1790 2092 2473 537
Size: 19 545 359 116 214 302 381 2590
Fragments arranged by size:
2590 545 381 359 302 214 116 19
BsrBI CCG'CTC
Cuts at: 50 2818 2872 4356 4521 50
Size: 2768 54 1484 165 55
Fragments arranged by size:
2768 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1431 2438 1431
Size: 1007 3519
BsrFI r'CCGG_y
Cuts at: 2524 2705 2914 2524
Size: 181 209 4136
Fragments arranged by size:
4136 209 181
BssSI C'ACGA_G
Cuts at: 2797 3155 2797
Size: 358 4168
BstAPI GCAn_nnn'nTGC
Cuts at: 1903 1903
Size: 4526
BstBI TT'CG_AA
Cuts at: 2887 2887
Size: 4526
BstYI r'GATC_y
Cuts at: 1 1393 1551 2018 2041 2376 2622 3634 3720
Size: 1392 158 467 23 335 246 1012 86
Cuts at: 3720 3732 4078 1
Size: 12 346 449
Fragments arranged by size:
1392 1012 467 449 346 335 246 158
86 23 12
BtgI C'CryG_G
Cuts at: 107 1359 107
Size: 1252 3274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2476 2657 2820 2476
Size: 181 163 4182
Fragments arranged by size:
4182 181 163
BtsI GCAGTG_nn'
Cuts at: 401 1036 1493 1769 401
Size: 635 457 276 3158
Fragments arranged by size:
3158 635 457 276
DraI TTT'AAA
Cuts at: 600 1525 600
Size: 925 3601
DrdI GACnn_nn'nnGTC
Cuts at: 771 2232 3090 771
Size: 1461 858 2207
Fragments arranged by size:
2207 1461 858
EaeI y'GGCC_r
Cuts at: 2111 2285 2676 2703 4048 2111
Size: 174 391 27 1345 2589
Fragments arranged by size:
2589 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4526
EarI CTCTTCn'nnn_
Cuts at: 263 660 753 793 2549 2759 263
Size: 397 93 40 1756 210 2030
Fragments arranged by size:
2030 1756 397 210 93 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 227 777 1171 1214 2734 3044 3190 4336 227
Size: 550 394 43 1520 310 146 1146 417
Fragments arranged by size:
1520 1146 550 417 394 310 146 43
EcoICRI GAG'CTC
Cuts at: 1538 1538
Size: 4526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 737 1069 1192 1310 1314 2351 2783 3530
Size: 228 332 123 118 4 1037 432 747
Cuts at: 3530 3798 509
Size: 268 1237
Fragments arranged by size:
1237 1037 747 432 332 268 228 123
118 4
EcoNI CCTnn'n_nnAGG
Cuts at: 692 692
Size: 4526
EcoO109I rG'GnC_Cy
Cuts at: 911 1665 911
Size: 754 3772
EcoRI G'AATT_C
Cuts at: 720 1530 720
Size: 810 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 884 1344 1533 2545 884
Size: 460 189 1012 2865
Fragments arranged by size:
2865 1012 460 189
FspI TGC'GCA
Cuts at: 2307 2307
Size: 4526
HaeII r_GCGC'y
Cuts at: 242 654 1960 2208 3230 4209 242
Size: 412 1306 248 1022 979 559
Fragments arranged by size:
1306 1022 979 559 412 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 277 309 361 393 456 488 604
Size: 32 56 32 52 32 63 32 116
Cuts at: 604 636 2368 2398 2400 2430 189
Size: 32 1732 30 2 30 2285
Fragments arranged by size:
2285 1732 116 63 56 52 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1565 1565
Size: 4526
KasI G'GCGC_C
Cuts at: 1956 2204 1956
Size: 248 4278
KpnI G_GTAC'C
Cuts at: 809 1546 809
Size: 737 3789
MluI A'CGCG_T
Cuts at: 3959 3959
Size: 4526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3197 3381 3197
Size: 184 4342
MscI TGG'CCA
Cuts at: 2287 2287
Size: 4526
MslI CAynn'nnrTG
Cuts at: 949 1158 2515 2642 949
Size: 209 1357 127 2833
Fragments arranged by size:
2833 1357 209 127
MspA1I CmG'CkG
Cuts at: 352 487 1637 1951 2311 3324 3569 352
Size: 135 1150 314 360 1013 245 1309
Fragments arranged by size:
1309 1150 1013 360 314 245 135
NaeI GCC'GGC
Cuts at: 2707 2707
Size: 4526
NarI GG'CG_CC
Cuts at: 1957 2205 1957
Size: 248 4278
NcoI C'CATG_G
Cuts at: 107 1359 107
Size: 1252 3274
NgoMIV G'CCGG_C
Cuts at: 2705 2705
Size: 4526
NspI r_CATG'y
Cuts at: 1342 1579 2079 2986 1342
Size: 237 500 907 2882
Fragments arranged by size:
2882 907 500 237
PasI CC'CwG_GG
Cuts at: 826 826
Size: 4526
PciI A'CATG_T
Cuts at: 2982 2982
Size: 4526
PfoI T'CCnGG_A
Cuts at: 888 888
Size: 4526
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4494
PpuMI rG'GwC_Cy
Cuts at: 911 911
Size: 4526
PstI C_TGCA'G
Cuts at: 471 1573 2258 471
Size: 1102 685 2739
Fragments arranged by size:
2739 1102 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4526
PvuII CAG'CTG
Cuts at: 352 487 1951 2311 352
Size: 135 1464 360 2567
Fragments arranged by size:
2567 1464 360 135
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4526
SacI G_AGCT'C
Cuts at: 1540 1540
Size: 4526
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4526
SapI GCTCTTCn'nnn_
Cuts at: 660 2549 2759 660
Size: 1889 210 2427
Fragments arranged by size:
2427 1889 210
SbfI CC_TGCA'GG
Cuts at: 1573 1573
Size: 4526
ScaI AGT'ACT
Cuts at: 4043 4043
Size: 4526
SexAI A'CCwGG_T
Cuts at: 769 769
Size: 4526
SfcI C'TryA_G
Cuts at: 33 413 467 1569 2254 3247 3438 33
Size: 380 54 1102 685 993 191 1121
Fragments arranged by size:
1121 1102 993 685 380 191 54
SfoI GGC'GCC
Cuts at: 1958 2206 1958
Size: 248 4278
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
SmaI CCC'GGG
Cuts at: 1548 1548
Size: 4526
SmlI C'TyrA_G
Cuts at: 1681 2596 3088 3350 1681
Size: 915 492 262 2857
Fragments arranged by size:
2857 915 492 262
SnaBI TAC'GTA
Cuts at: 982 982
Size: 4526
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4526
SphI G_CATG'C
Cuts at: 1342 1579 2079 1342
Size: 237 500 3789
Fragments arranged by size:
3789 500 237
StyI C'CwwG_G
Cuts at: 107 1359 1660 2014 107
Size: 1252 301 354 2619
Fragments arranged by size:
2619 1252 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 831 2109 2242 2701 2910 831
Size: 1278 133 459 209 2447
Fragments arranged by size:
2447 1278 459 209 133
TatI w'GTAC_w
Cuts at: 4041 4041
Size: 4526
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4043 4075 4043
Size: 32 4494
Tth111I GACn'n_nGTC
Cuts at: 616 2323 616
Size: 1707 2819
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1192 1255 2021 1192
Size: 63 766 3697
Fragments arranged by size:
3697 766 63
XmaI C'CCGG_G
Cuts at: 1546 1546
Size: 4526
XmnI GAAnn'nnTTC
Cuts at: 724 724
Size: 4526
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI