Restriction map for
pF1KB4249
(Circular) (Six-base) MAPSORT of: pF1KB4249.seq Check: 9504 from: 1 to: 4199
pF1KB4249 4199 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 973 973
Size: 4199
AccI GT'mk_AC
Cuts at: 1237 1237
Size: 4199
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 2024 2456 3203 3471 676
Size: 1348 432 747 268 1404
Fragments arranged by size:
1404 1348 747 432 268
AflIII A'CryG_T
Cuts at: 1551 2655 3632 1551
Size: 1104 977 2118
Fragments arranged by size:
2118 1104 977
AgeI A'CCGG_T
Cuts at: 2587 2587
Size: 4199
AlwNI CAG_nnn'CTG
Cuts at: 182 1014 1504 3071 182
Size: 832 490 1567 1310
Fragments arranged by size:
1567 1310 832 490
ApaLI G'TGCA_C
Cuts at: 1024 2969 1024
Size: 1945 2254
ApoI r'AATT_y
Cuts at: 1203 3552 3808 1203
Size: 2349 256 1594
Fragments arranged by size:
2349 1594 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 709 1219 709
Size: 510 3689
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 1215 1629 1877 1912 1215
Size: 414 248 35 3502
Fragments arranged by size:
3502 414 248 35
BanII G_rGCy'C
Cuts at: 452 1127 1213 2243 452
Size: 675 86 1030 2408
Fragments arranged by size:
2408 1030 675 86
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BbsI GAAGACnn'nnnn_
Cuts at: 664 789 879 664
Size: 125 90 3984
Fragments arranged by size:
3984 125 90
BciVI GTATCCnnnnn_n'
Cuts at: 2097 2858 4199 2097
Size: 761 1341 2097
Fragments arranged by size:
2097 1341 761
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglI GCCn_nnn'nGGC
Cuts at: 826 826
Size: 4199
BglII A'GATC_T
Cuts at: 1714 3751 1714
Size: 2037 2162
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 1028 1824 1917 2973 1028
Size: 796 93 1056 2254
Fragments arranged by size:
2254 1056 796 93
BmgBI CAC'GTC
Cuts at: 668 668
Size: 4199
BmrI ACTGGGnnnn_n'
Cuts at: 394 1411 1492 1827 3971 394
Size: 1017 81 335 2144 622
Fragments arranged by size:
2144 1017 622 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 768 2566 768
Size: 1798 2401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1131 994
Size: 137 4062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 2254 2746 3044 1375
Size: 879 492 298 2530
Fragments arranged by size:
2530 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 679 756 3903 679
Size: 77 3147 975
Fragments arranged by size:
3147 975 77
BsaAI yAC'GTr
Cuts at: 455 515 879 915 1552 455
Size: 60 364 36 637 3102
Fragments arranged by size:
3102 637 364 60 36
BsaBI GATnn'nnATC
Cuts at: 6 116 1713 3750 6
Size: 110 1597 2037 455
Fragments arranged by size:
2037 1597 455 110
BsaHI Gr'CG_yC
Cuts at: 829 1630 1878 2580 4076 829
Size: 801 248 702 1496 952
Fragments arranged by size:
1496 952 801 702 248
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2861 3008 3419 1388
Size: 521 678 274 147 411 2168
Fragments arranged by size:
2168 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 922 952 232
Size: 30 660 30 3479
Fragments arranged by size:
3479 660 30 30
BseYI C'CCAG_C
Cuts at: 565 1624 2382 2959 3455 565
Size: 1059 758 577 496 1309
Fragments arranged by size:
1309 1059 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 931 442
Size: 489 3710
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 452 716 980 1028 1189 1213 1991 2181 2973
Size: 264 264 48 161 24 778 190 792
Cuts at: 2973 452
Size: 1678
Fragments arranged by size:
1678 792 778 264 264 190 161 48
24
BsmBI CGTCTCn'nnnn_
Cuts at: 1153 1153
Size: 4199
Bsp1286I G_dGCh'C
Cuts at: 452 716 980 1028 1127 1189 1213 1824 1917
Size: 264 264 48 99 62 24 611 93
Cuts at: 1917 1991 2181 2243 2973 452
Size: 74 190 62 730 1678
Fragments arranged by size:
1678 730 611 264 264 190 99 93
74 62 62 48 24
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 3375 3375
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1249 1463 1765 2146 973
Size: 276 214 302 381 3026
Fragments arranged by size:
3026 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 368 2111 368
Size: 1743 2456
BsrFI r'CCGG_y
Cuts at: 695 968 2197 2378 2587 695
Size: 273 1229 181 209 2307
Fragments arranged by size:
2307 1229 273 209 181
BssSI C'ACGA_G
Cuts at: 418 2470 2828 418
Size: 2052 358 1789
Fragments arranged by size:
2052 1789 358
BstAPI GCAn_nnn'nTGC
Cuts at: 182 1576 182
Size: 1394 2805
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 297 297
Size: 4199
BstYI r'GATC_y
Cuts at: 1 111 1224 1691 1714 2049 2295 3307 3393
Size: 110 1113 467 23 335 246 1012 86
Cuts at: 3393 3405 3751 1
Size: 12 346 449
Fragments arranged by size:
1113 1012 467 449 346 335 246 110
86 23 12
BtgI C'CryG_G
Cuts at: 107 296 440 107
Size: 189 144 3866
Fragments arranged by size:
3866 189 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 345 1026 1442 345
Size: 681 416 3102
Fragments arranged by size:
3102 681 416
ClaI AT'CG_AT
Cuts at: 1139 1139
Size: 4199
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DraIII CAC_nnn'GTG
Cuts at: 1010 1010
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 666 1905 2763 666
Size: 1239 858 2102
Fragments arranged by size:
2102 1239 858
EaeI y'GGCC_r
Cuts at: 163 365 812 1112 1784 1958 2349 2376 3721
Size: 202 447 300 672 174 391 27 1345
Cuts at: 3721 163
Size: 641
Fragments arranged by size:
1345 672 641 447 391 300 202 174
27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 127 496 991 2222 2432 127
Size: 369 495 1231 210 1894
Fragments arranged by size:
1894 1231 495 369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2407 2717 2863 4009 2407
Size: 310 146 1146 2597
Fragments arranged by size:
2597 1146 310 146
EcoICRI GAG'CTC
Cuts at: 450 1211 450
Size: 761 3438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 994 1131 2024 2456 3203 3471 676
Size: 318 137 893 432 747 268 1404
Fragments arranged by size:
1404 893 747 432 318 268 137
EcoO109I rG'GnC_Cy
Cuts at: 1338 1338
Size: 4199
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 885 2218 885
Size: 1333 2866
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 1633 1881 2903 3882 1633
Size: 248 1022 979 1950
Fragments arranged by size:
1950 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 922 954 2041 2071 2073 2103 922
Size: 32 1087 30 2 30 3018
Fragments arranged by size:
3018 1087 32 30 30 2
HincII GTy'rAC
Cuts at: 1038 1238 1038
Size: 200 3999
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2870 3054 701
Size: 2169 184 1846
Fragments arranged by size:
2169 1846 184
MscI TGG'CCA
Cuts at: 165 367 814 1960 165
Size: 202 447 1146 2404
Fragments arranged by size:
2404 1146 447 202
MslI CAynn'nnrTG
Cuts at: 190 202 280 517 523 607 2188 2315 190
Size: 12 78 237 6 84 1581 127 2074
Fragments arranged by size:
2074 1581 237 127 84 78 12 6
MspA1I CmG'CkG
Cuts at: 161 1310 1624 1984 2997 3242 161
Size: 1149 314 360 1013 245 1118
Fragments arranged by size:
1149 1118 1013 360 314 245
NaeI GCC'GGC
Cuts at: 970 2380 970
Size: 1410 2789
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NcoI C'CATG_G
Cuts at: 107 440 107
Size: 333 3866
NgoMIV G'CCGG_C
Cuts at: 968 2378 968
Size: 1410 2789
NspI r_CATG'y
Cuts at: 219 1003 1252 1752 2659 219
Size: 784 249 500 907 1759
Fragments arranged by size:
1759 907 784 500 249
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PmlI CAC'GTG
Cuts at: 455 515 915 455
Size: 60 400 3739
Fragments arranged by size:
3739 400 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 161 1624 1984 161
Size: 1463 360 2376
Fragments arranged by size:
2376 1463 360
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 452 1213 452
Size: 761 3438
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 2222 2432 2222
Size: 210 3989
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 1108 1168 3716 1108
Size: 60 2548 1591
Fragments arranged by size:
2548 1591 60
SfcI C'TryA_G
Cuts at: 33 1050 1071 1242 1927 2920 3111 33
Size: 1017 21 171 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 191 171 21
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 1354 2269 2761 3023 1354
Size: 915 492 262 2530
Fragments arranged by size:
2530 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
StuI AGG'CCT
Cuts at: 1093 1093
Size: 4199
StyI C'CwwG_G
Cuts at: 107 440 1175 1333 1687 107
Size: 333 735 158 354 2619
Fragments arranged by size:
2619 735 354 333 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 396 1782 1915 2374 2583 297
Size: 99 1386 133 459 209 1913
Fragments arranged by size:
1913 1386 459 209 133 99
TatI w'GTAC_w
Cuts at: 1106 1166 3714 1106
Size: 60 2548 1591
Fragments arranged by size:
2548 1591 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 1694 297
Size: 1397 2802
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
XmnI GAAnn'nnTTC
Cuts at: 1093 1093
Size: 4199
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI