Restriction map for
pF1KB4264
(Circular) (Six-base) MAPSORT of: pF1KB4264.seq Check: 9332 from: 1 to: 5246
pF1KB4264 5246 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 903 937 2284 903
Size: 34 1347 3865
Fragments arranged by size:
3865 1347 34
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5246
AclI AA'CG_TT
Cuts at: 5086 5086
Size: 5246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 845 899 1225 1562 1903 3071 3503 3856
Size: 600 54 326 337 341 1168 432 353
Cuts at: 3856 4518 245
Size: 662 973
Fragments arranged by size:
1168 973 662 600 432 353 341 337
326 54
AflII C'TTAA_G
Cuts at: 115 742 115
Size: 627 4619
AflIII A'CryG_T
Cuts at: 2598 4398 4679 2598
Size: 1800 281 3165
Fragments arranged by size:
3165 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 630 1380 630
Size: 750 4496
AlwNI CAG_nnn'CTG
Cuts at: 1540 2551 3989 1540
Size: 1011 1438 2797
Fragments arranged by size:
2797 1438 1011
ApaI G_GGCC'C
Cuts at: 1440 1440
Size: 5246
ApaLI G'TGCA_C
Cuts at: 4084 4084
Size: 5246
ApoI r'AATT_y
Cuts at: 1247 1780 2063 2105 2250 4599 4855 1247
Size: 533 283 42 145 2349 256 1638
Fragments arranged by size:
2349 1638 533 283 256 145 42
AseI AT'TA_AT
Cuts at: 20 1634 20
Size: 1614 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
AvaI C'yCGr_G
Cuts at: 2266 2266
Size: 5246
BamHI G'GATC_C
Cuts at: 2271 2271
Size: 5246
BanI G'GyrC_C
Cuts at: 2262 2676 2924 2959 2262
Size: 414 248 35 4549
Fragments arranged by size:
4549 414 248 35
BanII G_rGCy'C
Cuts at: 1440 2260 3290 1440
Size: 820 1030 3396
Fragments arranged by size:
3396 1030 820
BbeI G_GCGC'C
Cuts at: 2680 2928 2680
Size: 248 4998
BbvCI CC'TCA_GC
Cuts at: 2194 2194
Size: 5246
BciVI GTATCCnnnnn_n'
Cuts at: 378 3144 4200 5246 378
Size: 2766 1056 1046 378
Fragments arranged by size:
2766 1056 1046 378
BclI T'GATC_A
Cuts at: 1929 1943 2766 1929
Size: 14 823 4409
Fragments arranged by size:
4409 823 14
BfrBI ATG'CAT
Cuts at: 978 978
Size: 5246
BglI GCCn_nnn'nGGC
Cuts at: 1493 1493
Size: 5246
BglII A'GATC_T
Cuts at: 1824 2761 4798 1824
Size: 937 2037 2272
Fragments arranged by size:
2272 2037 937
BlpI GC'TnA_GC
Cuts at: 251 2358 251
Size: 2107 3139
Bme1580I G_kGCm'C
Cuts at: 180 1440 2871 2964 4088 180
Size: 1260 1431 93 1124 1338
Fragments arranged by size:
1431 1338 1260 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 550 1042 2458 2539 2874 5018 550
Size: 492 1416 81 335 2144 778
Fragments arranged by size:
2144 1416 778 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3613 3613
Size: 5246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 1451 350
Size: 1101 4145
Bpu10I CC'TnA_GC
Cuts at: 2194 2194
Size: 5246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2422 3301 4015 4313 2422
Size: 879 714 298 3355
Fragments arranged by size:
3355 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 574 4950 574
Size: 4376 870
BsaAI yAC'GTr
Cuts at: 2599 2599
Size: 5246
BsaBI GATnn'nnATC
Cuts at: 6 2760 4797 6
Size: 2754 2037 455
Fragments arranged by size:
2754 2037 455
BsaHI Gr'CG_yC
Cuts at: 2677 2925 3627 5123 2677
Size: 248 702 1496 2800
Fragments arranged by size:
2800 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1230 2435 2956 3634 4045 4192 4466 1230
Size: 1205 521 678 411 147 274 2010
Fragments arranged by size:
2010 1205 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1397 1427 1838 1868 1397
Size: 30 411 30 4775
Fragments arranged by size:
4775 411 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1439 1439
Size: 5246
BseYI C'CCAG_C
Cuts at: 1401 1528 2671 3429 4094 4502 1401
Size: 127 1143 758 665 408 2145
Fragments arranged by size:
2145 1143 758 665 408 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1862 521
Size: 1341 3905
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4064 104
Size: 2684 46 1230 1286
Fragments arranged by size:
2684 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 131 1578 2260 3038 3228 4088 131
Size: 1447 682 778 190 860 1289
Fragments arranged by size:
1447 1289 860 778 682 190
BsmI GAATG_Cn'
Cuts at: 890 890
Size: 5246
Bsp1286I G_dGCh'C
Cuts at: 131 180 1440 1578 2260 2871 2964 3038 3228
Size: 49 1260 138 682 611 93 74 190
Cuts at: 3228 3290 4088 131
Size: 62 798 1289
Fragments arranged by size:
1289 1260 798 682 611 190 138 93
74 62 49
BspEI T'CCGG_A
Cuts at: 1230 1230
Size: 5246
BspHI T'CATG_A
Cuts at: 1466 3678 1466
Size: 2212 3034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 2296 2510 2812 3193 622
Size: 1674 214 302 381 2675
Fragments arranged by size:
2675 1674 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3538 3592 5076 5241 50
Size: 3488 54 1484 165 55
Fragments arranged by size:
3488 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 563 3158 563
Size: 2595 2651
BsrFI r'CCGG_y
Cuts at: 3244 3425 3244
Size: 181 5065
BssSI C'ACGA_G
Cuts at: 3517 4225 3517
Size: 708 4538
BstAPI GCAn_nnn'nTGC
Cuts at: 2623 2623
Size: 5246
BstBI TT'CG_AA
Cuts at: 3607 3607
Size: 5246
BstXI CCAn_nnnn'nTGG
Cuts at: 1218 1504 1792 2091 2138 1218
Size: 286 288 299 47 4326
Fragments arranged by size:
4326 299 288 286 47
BstYI r'GATC_y
Cuts at: 1 868 1824 2271 2738 2761 3096 3342 3648
Size: 867 956 447 467 23 335 246 306
Cuts at: 3648 3660 3746 4798 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 956 867 467 449 447 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 938 938
Size: 5246
Bsu36I CC'TnA_GG
Cuts at: 962 962
Size: 5246
BtgI C'CryG_G
Cuts at: 193 1688 2137 193
Size: 1495 449 3302
Fragments arranged by size:
3302 1495 449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3196 3377 3540 3196
Size: 181 163 4902
Fragments arranged by size:
4902 181 163
BtsI GCAGTG_nn'
Cuts at: 533 2489 533
Size: 1956 3290
DraI TTT'AAA
Cuts at: 1383 1701 2245 1383
Size: 318 544 4384
Fragments arranged by size:
4384 544 318
DrdI GACnn_nn'nnGTC
Cuts at: 2952 4296 2952
Size: 1344 3902
EaeI y'GGCC_r
Cuts at: 923 954 1494 2831 3005 3396 3423 4768 923
Size: 31 540 1337 174 391 27 1345 1401
Fragments arranged by size:
1401 1345 1337 540 391 174 31 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5246
EarI CTCTTCn'nnn_
Cuts at: 854 1703 3269 3479 854
Size: 849 1566 210 2621
Fragments arranged by size:
2621 1566 849 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3454 4196 4342 5056 3454
Size: 742 146 714 3644
Fragments arranged by size:
3644 742 714 146
EcoICRI GAG'CTC
Cuts at: 2258 2258
Size: 5246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 350 845 899 1225 1451 1562 1903 3071
Size: 105 495 54 326 226 111 341 1168
Cuts at: 3071 3503 3856 4518 245
Size: 432 353 662 973
Fragments arranged by size:
1168 973 662 495 432 353 341 326
226 111 105 54
EcoO109I rG'GnC_Cy
Cuts at: 1395 1436 1437 2385 1395
Size: 41 1 948 4256
Fragments arranged by size:
4256 948 41 1
EcoRI G'AATT_C
Cuts at: 1780 2250 1780
Size: 470 4776
EcoRV GAT'ATC
Cuts at: 762 1100 2022 762
Size: 338 922 3986
Fragments arranged by size:
3986 922 338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 645 3265 645
Size: 2620 2626
FspI TGC'GCA
Cuts at: 3027 3027
Size: 5246
HaeII r_GCGC'y
Cuts at: 2680 2928 4158 4929 2680
Size: 248 1230 771 2997
Fragments arranged by size:
2997 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 474 506 853 885 963 995 1397 1429 1646
Size: 32 347 32 78 32 402 32 217
Cuts at: 1646 1667 1678 1699 1797 1829 1840 1872 3088
Size: 21 11 21 98 32 11 32 1216
Cuts at: 3088 3118 3120 3150 474
Size: 30 2 30 2570
Fragments arranged by size:
2570 1216 402 347 217 98 78 32
32 32 32 32 32 30 30 21
21 11 11 2
HincII GTy'rAC
Cuts at: 1976 2285 1976
Size: 309 4937
KasI G'GCGC_C
Cuts at: 2676 2924 2676
Size: 248 4998
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5246
MfeI C'AATT_G
Cuts at: 1490 1490
Size: 5246
MluI A'CGCG_T
Cuts at: 4679 4679
Size: 5246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1551 4005 4189 1551
Size: 2454 184 2608
Fragments arranged by size:
2608 2454 184
MscI TGG'CCA
Cuts at: 925 1496 3007 925
Size: 571 1511 3164
Fragments arranged by size:
3164 1511 571
MslI CAynn'nnrTG
Cuts at: 787 931 1216 1687 1790 3235 3362 787
Size: 144 285 471 103 1445 127 2671
Fragments arranged by size:
2671 1445 471 285 144 127 103
MspA1I CmG'CkG
Cuts at: 2357 2671 3031 3815 4060 2357
Size: 314 360 784 245 3543
Fragments arranged by size:
3543 784 360 314 245
NaeI GCC'GGC
Cuts at: 3427 3427
Size: 5246
NarI GG'CG_CC
Cuts at: 2677 2925 2677
Size: 248 4998
NcoI C'CATG_G
Cuts at: 193 1688 2137 193
Size: 1495 449 3302
Fragments arranged by size:
3302 1495 449
NgoMIV G'CCGG_C
Cuts at: 3425 3425
Size: 5246
NsiI A_TGCA'T
Cuts at: 980 980
Size: 5246
NspI r_CATG'y
Cuts at: 849 2299 2799 4402 849
Size: 1450 500 1603 1693
Fragments arranged by size:
1693 1603 1450 500
PciI A'CATG_T
Cuts at: 4398 4398
Size: 5246
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 5214
PsiI TTA'TAA
Cuts at: 476 1253 476
Size: 777 4469
PspOMI G'GGCC_C
Cuts at: 1436 1436
Size: 5246
PstI C_TGCA'G
Cuts at: 1123 2293 2978 1123
Size: 1170 685 3391
Fragments arranged by size:
3391 1170 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5246
PvuII CAG'CTG
Cuts at: 2671 3031 2671
Size: 360 4886
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5246
SacI G_AGCT'C
Cuts at: 2260 2260
Size: 5246
SalI G'TCGA_C
Cuts at: 2283 2283
Size: 5246
SapI GCTCTTCn'nnn_
Cuts at: 3269 3479 3269
Size: 210 5036
SbfI CC_TGCA'GG
Cuts at: 2293 2293
Size: 5246
ScaI AGT'ACT
Cuts at: 4763 4763
Size: 5246
SfcI C'TryA_G
Cuts at: 33 1119 2289 2974 3942 4133 33
Size: 1086 1170 685 968 191 1146
Fragments arranged by size:
1170 1146 1086 968 685 191
SfoI GGC'GCC
Cuts at: 2678 2926 2678
Size: 248 4998
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
SmaI CCC'GGG
Cuts at: 2268 2268
Size: 5246
SmlI C'TyrA_G
Cuts at: 115 742 2401 3316 4030 4292 115
Size: 627 1659 915 714 262 1069
Fragments arranged by size:
1659 1069 915 714 627 262
SpeI A'CTAG_T
Cuts at: 67 940 67
Size: 873 4373
SphI G_CATG'C
Cuts at: 849 2299 2799 849
Size: 1450 500 3296
Fragments arranged by size:
3296 1450 500
StyI C'CwwG_G
Cuts at: 193 393 1688 2137 2380 2734 193
Size: 200 1295 449 243 354 2705
Fragments arranged by size:
2705 1295 449 354 243 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1053 2829 2962 3421 3630 1053
Size: 1776 133 459 209 2669
Fragments arranged by size:
2669 1776 459 209 133
TatI w'GTAC_w
Cuts at: 623 1195 1200 4761 623
Size: 572 5 3561 1108
Fragments arranged by size:
3561 1108 572 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 5214
Tth111I GACn'n_nGTC
Cuts at: 3043 3043
Size: 5246
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2741 2741
Size: 5246
XmaI C'CCGG_G
Cuts at: 2266 2266
Size: 5246
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI AscI
AvrII BaeI BbsI BcgI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI