Restriction map for
pF1KB4266
(Circular) (Six-base) MAPSORT of: pF1KB4266.seq Check: 2379 from: 1 to: 4646
pF1KB4266 4646 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1684 1684
Size: 4646
Acc65I G'GTAC_C
Cuts at: 1662 1662
Size: 4646
AclI AA'CG_TT
Cuts at: 785 4486 785
Size: 3701 945
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2903 3650 3918 2471
Size: 432 747 268 3199
Fragments arranged by size:
3199 747 432 268
AfeI AGC'GCT
Cuts at: 748 748
Size: 4646
AflIII A'CryG_T
Cuts at: 350 1322 1567 1998 3102 4079 350
Size: 972 245 431 1104 977 917
Fragments arranged by size:
1104 977 972 917 431 245
AgeI A'CCGG_T
Cuts at: 3034 3034
Size: 4646
AleI CACnn'nnGTG
Cuts at: 802 802
Size: 4646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 820 820
Size: 4646
AlwNI CAG_nnn'CTG
Cuts at: 1951 3518 1951
Size: 1567 3079
ApaLI G'TGCA_C
Cuts at: 3416 3416
Size: 4646
ApoI r'AATT_y
Cuts at: 1589 1650 3999 4255 1589
Size: 61 2349 256 1980
Fragments arranged by size:
2349 1980 256 61
AseI AT'TA_AT
Cuts at: 20 20
Size: 4646
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 531 1666 531
Size: 1135 3511
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 614 1662 2076 2324 2359 614
Size: 1048 414 248 35 2901
Fragments arranged by size:
2901 1048 414 248 35
BanII G_rGCy'C
Cuts at: 227 416 1362 1660 2690 227
Size: 189 946 298 1030 2183
Fragments arranged by size:
2183 1030 946 298 189
BbeI G_GCGC'C
Cuts at: 618 2080 2328 618
Size: 1462 248 2936
Fragments arranged by size:
2936 1462 248
BbsI GAAGACnn'nnnn_
Cuts at: 288 288
Size: 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 189 223 1620 1654 189
Size: 34 1397 34 3181
Fragments arranged by size:
3181 1397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3305 4646 2544
Size: 761 1341 2544
Fragments arranged by size:
2544 1341 761
BclI T'GATC_A
Cuts at: 140 2166 140
Size: 2026 2620
BglI GCCn_nnn'nGGC
Cuts at: 217 290 457 602 217
Size: 73 167 145 4261
Fragments arranged by size:
4261 167 145 73
BglII A'GATC_T
Cuts at: 2161 4198 2161
Size: 2037 2609
BlpI GC'TnA_GC
Cuts at: 991 1451 1758 991
Size: 460 307 3879
Fragments arranged by size:
3879 460 307
Bme1580I G_kGCm'C
Cuts at: 1221 1406 2271 2364 3420 1221
Size: 185 865 93 1056 2447
Fragments arranged by size:
2447 1056 865 185 93
BmgBI CAC'GTC
Cuts at: 351 408 1023 1337 351
Size: 57 615 314 3660
Fragments arranged by size:
3660 615 314 57
BmrI ACTGGGnnnn_n'
Cuts at: 1858 1939 2274 4418 1858
Size: 81 335 2144 2086
Fragments arranged by size:
2144 2086 335 81
BmtI G_CTAG'C
Cuts at: 1130 1130
Size: 4646
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 4646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 754 1000 574
Size: 180 246 4220
Fragments arranged by size:
4220 246 180
Bpu10I CC'TnA_GC
Cuts at: 1440 1440
Size: 4646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2701 3193 3491 1822
Size: 879 492 298 2977
Fragments arranged by size:
2977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 394 717 730 4350 394
Size: 323 13 3620 690
Fragments arranged by size:
3620 690 323 13
BsaAI yAC'GTr
Cuts at: 1999 1999
Size: 4646
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 230 293 449 512 615 857 2077 2325 3027
Size: 63 156 63 103 242 1220 248 702
Cuts at: 3027 4523 230
Size: 1496 353
Fragments arranged by size:
1496 1220 702 353 248 242 156 103
63 63
BsaWI w'CCGG_w
Cuts at: 1835 2356 3034 3308 3455 3866 1835
Size: 521 678 274 147 411 2615
Fragments arranged by size:
2615 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 388 469 473 385
Size: 3 81 4 4558
Fragments arranged by size:
4558 81 4 3
BseYI C'CCAG_C
Cuts at: 342 1026 1234 1503 2071 2829 3406 3902 342
Size: 684 208 269 568 758 577 496 1086
Fragments arranged by size:
1086 758 684 577 568 496 269 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 4646
BsiEI CG_ry'CG
Cuts at: 104 282 1640 2188 2234 3442 104
Size: 178 1358 548 46 1208 1308
Fragments arranged by size:
1358 1308 1208 548 178 46
BsiHKAI G_wGCw'C
Cuts at: 1250 1385 1660 2438 2628 3420 1250
Size: 135 275 778 190 792 2476
Fragments arranged by size:
2476 792 778 275 190 135
BsmBI CGTCTCn'nnnn_
Cuts at: 820 820
Size: 4646
Bsp1286I G_dGCh'C
Cuts at: 227 416 1221 1250 1362 1385 1406 1660 2271
Size: 189 805 29 112 23 21 254 611
Cuts at: 2271 2364 2438 2628 2690 3420 227
Size: 93 74 190 62 730 1453
Fragments arranged by size:
1453 805 730 611 254 190 189 112
93 74 62 29 23 21
BspEI T'CCGG_A
Cuts at: 3866 3866
Size: 4646
BspHI T'CATG_A
Cuts at: 3822 3822
Size: 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1600 1696 1910 2212 2593 1600
Size: 96 214 302 381 3653
Fragments arranged by size:
3653 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 176 363 995 2938 2992 4476 4641 50
Size: 126 187 632 1943 54 1484 165 55
Fragments arranged by size:
1943 1484 632 187 165 126 55 54
BsrDI GCAATG_nn'
Cuts at: 798 2558 798
Size: 1760 2886
BsrFI r'CCGG_y
Cuts at: 596 2644 2825 3034 596
Size: 2048 181 209 2208
Fragments arranged by size:
2208 2048 209 181
BssSI C'ACGA_G
Cuts at: 742 2917 3275 742
Size: 2175 358 2113
Fragments arranged by size:
2175 2113 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2023 2023
Size: 4646
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstEII G'GTnAC_C
Cuts at: 1293 1293
Size: 4646
BstXI CCAn_nnnn'nTGG
Cuts at: 570 570
Size: 4646
BstYI r'GATC_y
Cuts at: 1 1507 1671 2138 2161 2496 2742 3754 3840
Size: 1506 164 467 23 335 246 1012 86
Cuts at: 3840 3852 4198 1
Size: 12 346 449
Fragments arranged by size:
1506 1012 467 449 346 335 246 164
86 23 12
BtgI C'CryG_G
Cuts at: 107 1289 1445 1489 107
Size: 1182 156 44 3264
Fragments arranged by size:
3264 1182 156 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1374 2596 2777 2940 1374
Size: 1222 181 163 3080
Fragments arranged by size:
3080 1222 181 163
BtsI GCAGTG_nn'
Cuts at: 336 1889 336
Size: 1553 3093
DraI TTT'AAA
Cuts at: 1645 1645
Size: 4646
DraIII CAC_nnn'GTG
Cuts at: 1092 1092
Size: 4646
DrdI GACnn_nn'nnGTC
Cuts at: 2352 3210 2352
Size: 858 3788
EaeI y'GGCC_r
Cuts at: 594 959 1492 2231 2405 2796 2823 4168 594
Size: 365 533 739 174 391 27 1345 1072
Fragments arranged by size:
1345 1072 739 533 391 365 174 27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 649 703 2669 2879 649
Size: 54 1966 210 2416
Fragments arranged by size:
2416 1966 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 235 2854 3164 3310 4456 235
Size: 2619 310 146 1146 425
Fragments arranged by size:
2619 1146 425 310 146
EcoICRI GAG'CTC
Cuts at: 1658 1658
Size: 4646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 754 1000 2471 2903 3650 3918 574
Size: 180 246 1471 432 747 268 1302
Fragments arranged by size:
1471 1302 747 432 268 246 180
EcoNI CCTnn'n_nnAGG
Cuts at: 1625 1625
Size: 4646
EcoO109I rG'GnC_Cy
Cuts at: 651 1104 1785 651
Size: 453 681 3512
Fragments arranged by size:
3512 681 453
EcoRI G'AATT_C
Cuts at: 1650 1650
Size: 4646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4646
FseI GG_CCGG'CC
Cuts at: 600 600
Size: 4646
FspI TGC'GCA
Cuts at: 187 1057 1066 2427 187
Size: 870 9 1361 2406
Fragments arranged by size:
2406 1361 870 9
FspAI rTGC'GCAy
Cuts at: 1057 1057
Size: 4646
HaeII r_GCGC'y
Cuts at: 482 500 570 577 618 643 750 1241 2080
Size: 18 70 7 41 25 107 491 839
Cuts at: 2080 2328 3350 4329 482
Size: 248 1022 979 799
Fragments arranged by size:
1022 979 839 799 491 248 107 70
41 25 18 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 2488 2518 2520 2550 643
Size: 32 1813 30 2 30 2739
Fragments arranged by size:
2739 1813 32 30 30 2
HincII GTy'rAC
Cuts at: 1685 1685
Size: 4646
KasI G'GCGC_C
Cuts at: 614 2076 2324 614
Size: 1462 248 2936
Fragments arranged by size:
2936 1462 248
KpnI G_GTAC'C
Cuts at: 1666 1666
Size: 4646
MluI A'CGCG_T
Cuts at: 4079 4079
Size: 4646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3317 3501 3317
Size: 184 4462
MscI TGG'CCA
Cuts at: 961 2407 961
Size: 1446 3200
MslI CAynn'nnrTG
Cuts at: 802 1351 1393 1399 2635 2762 802
Size: 549 42 6 1236 127 2686
Fragments arranged by size:
2686 1236 549 127 42 6
MspA1I CmG'CkG
Cuts at: 620 1491 1503 1575 1757 2071 2431 3444 3689
Size: 871 12 72 182 314 360 1013 245
Cuts at: 3689 620
Size: 1577
Fragments arranged by size:
1577 1013 871 360 314 245 182 72
12
NaeI GCC'GGC
Cuts at: 598 2827 598
Size: 2229 2417
NarI GG'CG_CC
Cuts at: 615 2077 2325 615
Size: 1462 248 2936
Fragments arranged by size:
2936 1462 248
NcoI C'CATG_G
Cuts at: 107 1445 107
Size: 1338 3308
NgoMIV G'CCGG_C
Cuts at: 596 2825 596
Size: 2229 2417
NheI G'CTAG_C
Cuts at: 1126 1126
Size: 4646
NspI r_CATG'y
Cuts at: 1326 1350 1571 1635 1699 2199 3106 1326
Size: 24 221 64 64 500 907 2866
Fragments arranged by size:
2866 907 500 221 64 64 24
PasI CC'CwG_GG
Cuts at: 1308 1308
Size: 4646
PciI A'CATG_T
Cuts at: 1322 1567 3102 1322
Size: 245 1535 2866
Fragments arranged by size:
2866 1535 245
PflMI CCAn_nnn'nTGG
Cuts at: 1092 1092
Size: 4646
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4614
PpuMI rG'GwC_Cy
Cuts at: 651 1104 651
Size: 453 4193
PshAI GACnn'nnGTC
Cuts at: 1568 1568
Size: 4646
PstI C_TGCA'G
Cuts at: 1693 2378 1693
Size: 685 3961
PvuI CG_AT'CG
Cuts at: 104 1640 104
Size: 1536 3110
PvuII CAG'CTG
Cuts at: 1503 2071 2431 1503
Size: 568 360 3718
Fragments arranged by size:
3718 568 360
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 1660 1660
Size: 4646
SacII CC_GC'GG
Cuts at: 1492 1492
Size: 4646
SalI G'TCGA_C
Cuts at: 1683 1683
Size: 4646
SapI GCTCTTCn'nnn_
Cuts at: 649 2669 2879 649
Size: 2020 210 2416
Fragments arranged by size:
2416 2020 210
SbfI CC_TGCA'GG
Cuts at: 1693 1693
Size: 4646
ScaI AGT'ACT
Cuts at: 1554 4163 1554
Size: 2609 2037
SfcI C'TryA_G
Cuts at: 33 1689 2374 3367 3558 33
Size: 1656 685 993 191 1121
Fragments arranged by size:
1656 1121 993 685 191
SfoI GGC'GCC
Cuts at: 616 2078 2326 616
Size: 1462 248 2936
Fragments arranged by size:
2936 1462 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SmaI CCC'GGG
Cuts at: 1668 1668
Size: 4646
SmlI C'TyrA_G
Cuts at: 1801 2716 3208 3470 1801
Size: 915 492 262 2977
Fragments arranged by size:
2977 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4646
SphI G_CATG'C
Cuts at: 1350 1699 2199 1350
Size: 349 500 3797
Fragments arranged by size:
3797 500 349
StuI AGG'CCT
Cuts at: 835 835
Size: 4646
StyI C'CwwG_G
Cuts at: 107 114 1007 1016 1166 1445 1780 2134 107
Size: 7 893 9 150 279 335 354 2619
Fragments arranged by size:
2619 893 354 335 279 150 9 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 418 2229 2362 2821 3030 418
Size: 1811 133 459 209 2034
Fragments arranged by size:
2034 1811 459 209 133
TatI w'GTAC_w
Cuts at: 1552 4161 1552
Size: 2609 2037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4163 4195 4163
Size: 32 4614
Tth111I GACn'n_nGTC
Cuts at: 1338 2443 1338
Size: 1105 3541
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 729 1023 1305 2141 729
Size: 294 282 836 3234
Fragments arranged by size:
3234 836 294 282
XmaI C'CCGG_G
Cuts at: 1666 1666
Size: 4646
XmnI GAAnn'nnTTC
Cuts at: 1420 1420
Size: 4646
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BsaXI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PfoI PmlI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI