Restriction map for
pF1KB4275
(Circular) (Six-base) MAPSORT of: pF1KB4275.seq Check: 4843 from: 1 to: 4835
pF1KB4275 4835 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 547 1083 547
Size: 536 4299
AccI GT'mk_AC
Cuts at: 1449 1873 1449
Size: 424 4411
Acc65I G'GTAC_C
Cuts at: 1561 1851 1561
Size: 290 4545
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 752 2660 3092 3445 4107 676
Size: 76 1908 432 353 662 1404
Fragments arranged by size:
1908 1404 662 432 353 76
AflIII A'CryG_T
Cuts at: 971 2187 3987 4268 971
Size: 1216 1800 281 1538
Fragments arranged by size:
1800 1538 1216 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1091 1511 1091
Size: 420 4415
AlwNI CAG_nnn'CTG
Cuts at: 736 1582 2140 3578 736
Size: 846 558 1438 1993
Fragments arranged by size:
1993 1438 846 558
ApaI G_GGCC'C
Cuts at: 1087 1087
Size: 4835
ApaLI G'TGCA_C
Cuts at: 289 3673 289
Size: 3384 1451
ApoI r'AATT_y
Cuts at: 441 826 1839 4188 4444 441
Size: 385 1013 2349 256 832
Fragments arranged by size:
2349 1013 832 385 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 214 497 1855 214
Size: 283 1358 3194
Fragments arranged by size:
3194 1358 283
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1684 1717 1684
Size: 33 4802
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 486 1071 1561 1658 1783 1851 2265 2513 2548
Size: 585 490 97 125 68 414 248 35
Cuts at: 2548 486
Size: 2773
Fragments arranged by size:
2773 585 490 414 248 125 97 68
35
BanII G_rGCy'C
Cuts at: 625 700 1087 1849 2879 625
Size: 75 387 762 1030 2581
Fragments arranged by size:
2581 1030 762 387 75
BbeI G_GCGC'C
Cuts at: 2269 2517 2269
Size: 248 4587
BbsI GAAGACnn'nnnn_
Cuts at: 570 570
Size: 4835
BbvCI CC'TCA_GC
Cuts at: 373 785 1089 373
Size: 412 304 4119
Fragments arranged by size:
4119 412 304
BciVI GTATCCnnnnn_n'
Cuts at: 1723 2733 3789 4835 1723
Size: 1010 1056 1046 1723
Fragments arranged by size:
1723 1056 1046 1010
BclI T'GATC_A
Cuts at: 1823 2355 1823
Size: 532 4303
BfrBI ATG'CAT
Cuts at: 1203 1203
Size: 4835
BglI GCCn_nnn'nGGC
Cuts at: 202 202
Size: 4835
BglII A'GATC_T
Cuts at: 2350 4387 2350
Size: 2037 2798
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 293 491 730 1087 1550 1608 1661 2460 2553
Size: 198 239 357 463 58 53 799 93
Cuts at: 2553 3677 293
Size: 1124 1451
Fragments arranged by size:
1451 1124 799 463 357 239 198 93
58 53
BmrI ACTGGGnnnn_n'
Cuts at: 1824 2047 2128 2463 4607 1824
Size: 223 81 335 2144 2052
Fragments arranged by size:
2144 2052 335 223 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 921 3202 921
Size: 2281 2554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 557 916 338
Size: 219 359 4257
Fragments arranged by size:
4257 359 219
Bpu10I CC'TnA_GC
Cuts at: 268 373 785 1089 268
Size: 105 412 304 4014
Fragments arranged by size:
4014 412 304 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 2011 2890 3604 3902 1538
Size: 473 879 714 298 2471
Fragments arranged by size:
2471 879 714 473 298
BsaI GGTCTCn'nnnn_
Cuts at: 4539 4539
Size: 4835
BsaAI yAC'GTr
Cuts at: 1545 2188 1545
Size: 643 4192
BsaBI GATnn'nnATC
Cuts at: 6 581 2349 4386 6
Size: 575 1768 2037 455
Fragments arranged by size:
2037 1768 575 455
BsaHI Gr'CG_yC
Cuts at: 2266 2514 3216 4712 2266
Size: 248 702 1496 2389
Fragments arranged by size:
2389 1496 702 248
BsaWI w'CCGG_w
Cuts at: 479 2024 2545 3223 3634 3781 4055 479
Size: 1545 521 678 411 147 274 1259
Fragments arranged by size:
1545 1259 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 339 903 1097 1807 339
Size: 564 194 710 3367
Fragments arranged by size:
3367 710 564 194
BseYI C'CCAG_C
Cuts at: 155 205 1043 1052 1162 1268 1740 2260 3018
Size: 50 838 9 110 106 472 520 758
Cuts at: 3018 3683 4091 155
Size: 665 408 899
Fragments arranged by size:
899 838 758 665 520 472 408 110
106 50 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1099
Size: 4835
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3653 104
Size: 2273 46 1230 1286
Fragments arranged by size:
2273 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 293 1107 1354 1849 2627 2817 3677 293
Size: 814 247 495 778 190 860 1451
Fragments arranged by size:
1451 860 814 778 495 247 190
BsmI GAATG_Cn'
Cuts at: 426 426
Size: 4835
Bsp1286I G_dGCh'C
Cuts at: 293 491 625 700 730 1087 1107 1354 1550
Size: 198 134 75 30 357 20 247 196
Cuts at: 1550 1608 1661 1849 2460 2553 2627 2817 2879
Size: 58 53 188 611 93 74 190 62
Cuts at: 2879 3677 293
Size: 798 1451
Fragments arranged by size:
1451 798 611 357 247 198 196 190
188 134 93 75 74 62 58 53
30 20
BspEI T'CCGG_A
Cuts at: 479 479
Size: 4835
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 547 1083 1885 2099 2401 2782 547
Size: 536 802 214 302 381 2600
Fragments arranged by size:
2600 802 536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3127 3181 4665 4830 50
Size: 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 2747 1493
Size: 1254 3581
BsrFI r'CCGG_y
Cuts at: 2833 3014 2833
Size: 181 4654
BssSI C'ACGA_G
Cuts at: 3106 3814 3106
Size: 708 4127
BstAPI GCAn_nnn'nTGC
Cuts at: 2212 2212
Size: 4835
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstEII G'GTnAC_C
Cuts at: 242 242
Size: 4835
BstYI r'GATC_y
Cuts at: 1 252 392 733 855 1796 1860 2327 2350
Size: 251 140 341 122 941 64 467 23
Cuts at: 2350 2685 2931 3237 3249 3335 4387 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 941 467 449 341 335 306 251
246 140 122 86 64 23 12
Bsu36I CC'TnA_GG
Cuts at: 668 668
Size: 4835
BtgI C'CryG_G
Cuts at: 107 625 107
Size: 518 4317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 293 658 758 1111 2078 293
Size: 365 100 353 967 3050
Fragments arranged by size:
3050 967 365 353 100
DraI TTT'AAA
Cuts at: 825 1370 1834 825
Size: 545 464 3826
Fragments arranged by size:
3826 545 464
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3885 2541
Size: 1344 3491
EaeI y'GGCC_r
Cuts at: 1196 1762 2420 2594 2985 3012 4357 1196
Size: 566 658 174 391 27 1345 1674
Fragments arranged by size:
1674 1345 658 566 391 174 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 912 2858 3068 912
Size: 1946 210 2679
Fragments arranged by size:
2679 1946 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1183 3043 3785 3931 4645 1183
Size: 1860 742 146 714 1373
Fragments arranged by size:
1860 1373 742 714 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 557 676 752 916 2660 3092 3445 4107
Size: 219 119 76 164 1744 432 353 662
Cuts at: 4107 338
Size: 1066
Fragments arranged by size:
1744 1066 662 432 353 219 164 119
76
EcoO109I rG'GnC_Cy
Cuts at: 790 1083 1084 1974 790
Size: 293 1 890 3651
Fragments arranged by size:
3651 890 293 1
EcoRI G'AATT_C
Cuts at: 441 1839 441
Size: 1398 3437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1395 2854 1395
Size: 1459 3376
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 2269 2517 3747 4518 2269
Size: 248 1230 771 2586
Fragments arranged by size:
2586 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 1336 1368 1377 1409 1431 1463 2677
Size: 32 733 32 9 32 22 32 1214
Cuts at: 2677 2707 2709 2739 571
Size: 30 2 30 2667
Fragments arranged by size:
2667 1214 733 32 32 32 32 30
30 22 9 2
HincII GTy'rAC
Cuts at: 1874 1874
Size: 4835
HindIII A'AGCT_T
Cuts at: 184 1377 184
Size: 1193 3642
KasI G'GCGC_C
Cuts at: 2265 2513 2265
Size: 248 4587
KpnI G_GTAC'C
Cuts at: 1565 1855 1565
Size: 290 4545
MfeI C'AATT_G
Cuts at: 530 1401 530
Size: 871 3964
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 415 3594 3778 415
Size: 3179 184 1472
Fragments arranged by size:
3179 1472 184
MscI TGG'CCA
Cuts at: 1198 2596 1198
Size: 1398 3437
MslI CAynn'nnrTG
Cuts at: 2824 2951 2824
Size: 127 4708
MspA1I CmG'CkG
Cuts at: 159 389 1093 1215 1946 2260 2620 3404 3649
Size: 230 704 122 731 314 360 784 245
Cuts at: 3649 159
Size: 1345
Fragments arranged by size:
1345 784 731 704 360 314 245 230
122
NaeI GCC'GGC
Cuts at: 3016 3016
Size: 4835
NarI GG'CG_CC
Cuts at: 2266 2514 2266
Size: 248 4587
NcoI C'CATG_G
Cuts at: 107 107
Size: 4835
NdeI CA'TA_TG
Cuts at: 1751 1751
Size: 4835
NgoMIV G'CCGG_C
Cuts at: 3014 3014
Size: 4835
NsiI A_TGCA'T
Cuts at: 1205 1205
Size: 4835
NspI r_CATG'y
Cuts at: 975 1888 2388 3991 975
Size: 913 500 1603 1819
Fragments arranged by size:
1819 1603 913 500
PasI CC'CwG_GG
Cuts at: 508 1080 508
Size: 572 4263
PciI A'CATG_T
Cuts at: 971 3987 971
Size: 3016 1819
PflMI CCAn_nnn'nTGG
Cuts at: 146 1780 146
Size: 1634 3201
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PmlI CAC'GTG
Cuts at: 1545 1545
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4803
PpuMI rG'GwC_Cy
Cuts at: 790 790
Size: 4835
PspOMI G'GGCC_C
Cuts at: 1083 1083
Size: 4835
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 952 984 952
Size: 32 4803
PstI C_TGCA'G
Cuts at: 1220 1882 2567 1220
Size: 662 685 3488
Fragments arranged by size:
3488 685 662
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 159 1093 2260 2620 159
Size: 934 1167 360 2374
Fragments arranged by size:
2374 1167 934 360
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SanDI GG'GwC_CC
Cuts at: 790 790
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 2858 3068 2858
Size: 210 4625
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 4352 4352
Size: 4835
SfcI C'TryA_G
Cuts at: 33 707 717 1094 1216 1878 2563 3531 3722
Size: 674 10 377 122 662 685 968 191
Cuts at: 3722 33
Size: 1146
Fragments arranged by size:
1146 968 685 674 662 377 191 122
10
SfoI GGC'GCC
Cuts at: 2267 2515 2267
Size: 248 4587
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 216 1857 216
Size: 1641 3194
SmlI C'TyrA_G
Cuts at: 1553 1990 2905 3619 3881 1553
Size: 437 915 714 262 2507
Fragments arranged by size:
2507 915 714 437 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1888 2388 1888
Size: 500 4335
SspI AAT'ATT
Cuts at: 1359 1359
Size: 4835
StyI C'CwwG_G
Cuts at: 107 1969 2323 107
Size: 1862 354 2619
Fragments arranged by size:
2619 1862 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 1676 1729 2418 2551 3010 3219 261
Size: 1415 53 689 133 459 209 1877
Fragments arranged by size:
1877 1415 689 459 209 133 53
TatI w'GTAC_w
Cuts at: 4350 4350
Size: 4835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 4803
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1681 2330 1681
Size: 649 4186
XmaI C'CCGG_G
Cuts at: 214 1855 214
Size: 1641 3194
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BcgI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NruI PacI PfoI
PshAI PsiI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI