Restriction map for
pF1KB4277
(Circular) (Six-base) MAPSORT of: pF1KB4277.seq Check: 6928 from: 1 to: 4388
pF1KB4277 4388 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 950 1370 2213 2645 2998 3660 680
Size: 270 420 843 432 353 662 1408
Fragments arranged by size:
1408 843 662 432 420 353 270
AflII C'TTAA_G
Cuts at: 1030 1030
Size: 4388
AflIII A'CryG_T
Cuts at: 1740 3540 3821 1740
Size: 1800 281 2307
Fragments arranged by size:
2307 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1135 1135
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 847 1693 3131 847
Size: 846 1438 2104
Fragments arranged by size:
2104 1438 846
ApaI G_GGCC'C
Cuts at: 498 498
Size: 4388
ApaLI G'TGCA_C
Cuts at: 3226 3226
Size: 4388
ApoI r'AATT_y
Cuts at: 418 1392 3741 3997 418
Size: 974 2349 256 809
Fragments arranged by size:
2349 974 809 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 1408 1408
Size: 4388
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 504 537 504
Size: 33 4355
BamHI G'GATC_C
Cuts at: 1362 1413 1362
Size: 51 4337
BanI G'GyrC_C
Cuts at: 1404 1818 2066 2101 1404
Size: 414 248 35 3691
Fragments arranged by size:
3691 414 248 35
BanII G_rGCy'C
Cuts at: 498 1402 2432 498
Size: 904 1030 2454
Fragments arranged by size:
2454 1030 904
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 772 1193 1298 1349 772
Size: 421 105 51 3811
Fragments arranged by size:
3811 421 105 51
BciVI GTATCCnnnnn_n'
Cuts at: 867 2286 3342 4388 867
Size: 1419 1056 1046 867
Fragments arranged by size:
1419 1056 1046 867
BclI T'GATC_A
Cuts at: 155 852 1908 155
Size: 697 1056 2635
Fragments arranged by size:
2635 1056 697
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 498 683 2013 2106 3230 498
Size: 185 1330 93 1124 1656
Fragments arranged by size:
1656 1330 1124 185 93
BmrI ACTGGGnnnn_n'
Cuts at: 243 1600 1681 2016 4160 243
Size: 1357 81 335 2144 471
Fragments arranged by size:
2144 1357 471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 620 247
Size: 373 4015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1564 2443 3157 3455 1564
Size: 879 714 298 2497
Fragments arranged by size:
2497 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3187 3334 3608 1577
Size: 521 678 411 147 274 2357
Fragments arranged by size:
2357 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 522 552 522
Size: 30 4358
BseYI C'CCAG_C
Cuts at: 235 1813 2571 3236 3644 235
Size: 1578 758 665 408 979
Fragments arranged by size:
1578 979 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 379
Size: 4388
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3206 104
Size: 1826 46 1230 1286
Fragments arranged by size:
1826 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1402 2180 2370 3230 1402
Size: 778 190 860 2560
Fragments arranged by size:
2560 860 778 190
BsmI GAATG_Cn'
Cuts at: 293 1038 293
Size: 745 3643
BsmBI CGTCTCn'nnnn_
Cuts at: 628 628
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 498 683 1402 2013 2106 2180 2370 2432 3230
Size: 185 719 611 93 74 190 62 798
Cuts at: 3230 498
Size: 1656
Fragments arranged by size:
1656 798 719 611 190 185 93 74
62
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 2386 2567 2386
Size: 181 4207
BssSI C'ACGA_G
Cuts at: 2659 3367 2659
Size: 708 3680
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstEII G'GTnAC_C
Cuts at: 1082 1082
Size: 4388
BstYI r'GATC_y
Cuts at: 1 591 1011 1362 1413 1880 1903 2238 2484
Size: 590 420 351 51 467 23 335 246
Cuts at: 2484 2790 2802 2888 3940 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 590 467 449 420 351 335 306
246 86 51 23 12
BtgI C'CryG_G
Cuts at: 107 627 107
Size: 520 3868
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 510 2338 2519 2682 510
Size: 1828 181 163 2216
Fragments arranged by size:
2216 1828 181 163
BtsI GCAGTG_nn'
Cuts at: 146 923 1631 146
Size: 777 708 2903
Fragments arranged by size:
2903 777 708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1184 1219 1184
Size: 35 4353
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 3438 2094
Size: 1344 3044
EaeI y'GGCC_r
Cuts at: 110 1973 2147 2538 2565 3910 110
Size: 1863 174 391 27 1345 588
Fragments arranged by size:
1863 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 3338 3484 4198 2596
Size: 742 146 714 2786
Fragments arranged by size:
2786 742 714 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 620 680 950 1370 2213 2645 2998 3660
Size: 373 60 270 420 843 432 353 662
Cuts at: 3660 247
Size: 975
Fragments arranged by size:
975 843 662 432 420 373 353 270
60
EcoO109I rG'GnC_Cy
Cuts at: 494 1527 494
Size: 1033 3355
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
EcoRV GAT'ATC
Cuts at: 1238 1238
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 789 2407 789
Size: 1618 2770
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3300 4071 1822
Size: 248 1230 771 2139
Fragments arranged by size:
2139 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 207 239 1121 1153 1185 2230 2260 2262 2292
Size: 32 882 32 32 1045 30 2 30
Cuts at: 2292 207
Size: 2303
Fragments arranged by size:
2303 1045 882 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3147 3331 3147
Size: 184 4204
MscI TGG'CCA
Cuts at: 2149 2149
Size: 4388
MslI CAynn'nnrTG
Cuts at: 1058 1306 2377 2504 1058
Size: 248 1071 127 2942
Fragments arranged by size:
2942 1071 248 127
MspA1I CmG'CkG
Cuts at: 988 1499 1813 2173 2957 3202 988
Size: 511 314 360 784 245 2174
Fragments arranged by size:
2174 784 511 360 314 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NspI r_CATG'y
Cuts at: 1441 1941 3544 1441
Size: 500 1603 2285
Fragments arranged by size:
2285 1603 500
PciI A'CATG_T
Cuts at: 3540 3540
Size: 4388
PflMI CCAn_nnn'nTGG
Cuts at: 225 225
Size: 4388
PfoI T'CCnGG_A
Cuts at: 1249 1249
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 4356
PshAI GACnn'nnGTC
Cuts at: 620 1377 620
Size: 757 3631
PsiI TTA'TAA
Cuts at: 1334 1334
Size: 4388
PspOMI G'GGCC_C
Cuts at: 494 494
Size: 4388
PstI C_TGCA'G
Cuts at: 1435 2120 1435
Size: 685 3703
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 988 1813 2173 988
Size: 825 360 3203
Fragments arranged by size:
3203 825 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 1431 2116 3084 3275 33
Size: 1398 685 968 191 1146
Fragments arranged by size:
1398 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1410 1410
Size: 4388
SmlI C'TyrA_G
Cuts at: 1030 1543 2458 3172 3434 1030
Size: 513 915 714 262 1984
Fragments arranged by size:
1984 915 714 513 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
SspI AAT'ATT
Cuts at: 1067 1067
Size: 4388
StyI C'CwwG_G
Cuts at: 107 1267 1522 1876 107
Size: 1160 255 354 2619
Fragments arranged by size:
2619 1160 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1260 1971 2104 2563 2772 1260
Size: 711 133 459 209 2876
Fragments arranged by size:
2876 711 459 209 133
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4356
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XmaI C'CCGG_G
Cuts at: 1408 1408
Size: 4388
XmnI GAAnn'nnTTC
Cuts at: 286 286
Size: 4388
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI