Restriction map for
pF1KB4278
(Circular) (Six-base) MAPSORT of: pF1KB4278.seq Check: 1590 from: 1 to: 4145
pF1KB4278 4145 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 176 1183 176
Size: 1007 3138
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1970 2402 3149 3417 611
Size: 1359 432 747 268 1339
Fragments arranged by size:
1359 1339 747 432 268
AflII C'TTAA_G
Cuts at: 901 901
Size: 4145
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaLI G'TGCA_C
Cuts at: 1089 2915 1089
Size: 1826 2319
ApoI r'AATT_y
Cuts at: 782 1149 3498 3754 782
Size: 367 2349 256 1173
Fragments arranged by size:
2349 1173 367 256
AseI AT'TA_AT
Cuts at: 20 815 20
Size: 795 3350
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 160 206 1165 160
Size: 46 959 3140
Fragments arranged by size:
3140 959 46
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 1161 1575 1823 1858 1161
Size: 414 248 35 3448
Fragments arranged by size:
3448 414 248 35
BanII G_rGCy'C
Cuts at: 1159 2189 1159
Size: 1030 3115
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbsI GAAGACnn'nnnn_
Cuts at: 458 458
Size: 4145
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1080 1665 1080
Size: 585 3560
BglII A'GATC_T
Cuts at: 897 1660 3697 897
Size: 763 2037 1345
Fragments arranged by size:
2037 1345 763
BlpI GC'TnA_GC
Cuts at: 668 1257 668
Size: 589 3556
Bme1580I G_kGCm'C
Cuts at: 1093 1770 1863 2919 1093
Size: 677 93 1056 2319
Fragments arranged by size:
2319 1056 677 93
BmrI ACTGGGnnnn_n'
Cuts at: 1070 1357 1438 1773 3917 1070
Size: 287 81 335 2144 1298
Fragments arranged by size:
2144 1298 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 858
Size: 4145
Bpu10I CC'TnA_GC
Cuts at: 971 971
Size: 4145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 2200 2692 2990 1321
Size: 879 492 298 2476
Fragments arranged by size:
2476 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 1659 3696 6
Size: 1653 2037 455
Fragments arranged by size:
2037 1653 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 485 1334 1855 2533 2807 2954 3365 485
Size: 849 521 678 274 147 411 1265
Fragments arranged by size:
1265 849 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1061 1066 1061
Size: 5 4140
BseYI C'CCAG_C
Cuts at: 1570 2328 2905 3401 1570
Size: 758 577 496 2314
Fragments arranged by size:
2314 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 1093 1159 1937 2127 2919 131
Size: 962 66 778 190 792 1357
Fragments arranged by size:
1357 962 792 778 190 66
Bsp1286I G_dGCh'C
Cuts at: 131 1093 1159 1770 1863 1937 2127 2189 2919
Size: 962 66 611 93 74 190 62 730
Cuts at: 2919 131
Size: 1357
Fragments arranged by size:
1357 962 730 611 190 93 74 66
62
BspEI T'CCGG_A
Cuts at: 485 3365 485
Size: 2880 1265
BspHI T'CATG_A
Cuts at: 141 474 3321 141
Size: 333 2847 965
Fragments arranged by size:
2847 965 333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 127 1195 1409 1711 2092 127
Size: 1068 214 302 381 2180
Fragments arranged by size:
2180 1068 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2057 2057
Size: 4145
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1522
Size: 4145
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstYI r'GATC_y
Cuts at: 1 319 897 1170 1637 1660 1995 2241 3253
Size: 318 578 273 467 23 335 246 1012
Cuts at: 3253 3339 3351 3697 1
Size: 86 12 346 449
Fragments arranged by size:
1012 578 467 449 346 335 318 273
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 989 989
Size: 4145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 1388
Size: 4145
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DraIII CAC_nnn'GTG
Cuts at: 260 260
Size: 4145
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 1730 1904 2295 2322 3667 1730
Size: 174 391 27 1345 2208
Fragments arranged by size:
2208 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 185 2168 2378 185
Size: 1983 210 1952
Fragments arranged by size:
1983 1952 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1157 1157
Size: 4145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 858 1970 2402 3149 3417 611
Size: 247 1112 432 747 268 1339
Fragments arranged by size:
1339 1112 747 432 268 247
EcoO109I rG'GnC_Cy
Cuts at: 1284 1284
Size: 4145
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1987 2017 2019 2049 1987
Size: 30 2 30 4083
Fragments arranged by size:
4083 30 30 2
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
HindIII A'AGCT_T
Cuts at: 677 677
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 177 225 2816 3000 177
Size: 48 2591 184 1322
Fragments arranged by size:
2591 1322 184 48
MscI TGG'CCA
Cuts at: 1906 1906
Size: 4145
MslI CAynn'nnrTG
Cuts at: 2134 2261 2134
Size: 127 4018
MspA1I CmG'CkG
Cuts at: 672 1256 1570 1930 2943 3188 672
Size: 584 314 360 1013 245 1629
Fragments arranged by size:
1629 1013 584 360 314 245
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NspI r_CATG'y
Cuts at: 1198 1698 2605 1198
Size: 500 907 2738
Fragments arranged by size:
2738 907 500
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PfoI T'CCnGG_A
Cuts at: 264 264
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PstI C_TGCA'G
Cuts at: 1192 1877 1192
Size: 685 3460
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 672 1570 1930 672
Size: 898 360 2887
Fragments arranged by size:
2887 898 360
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 1159 1159
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 960 1188 1873 2866 3057 33
Size: 927 228 685 993 191 1121
Fragments arranged by size:
1121 993 927 685 228 191
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 206 901 1300 2215 2707 2969 206
Size: 695 399 915 492 262 1382
Fragments arranged by size:
1382 915 695 492 399 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
SspI AAT'ATT
Cuts at: 354 462 354
Size: 108 4037
StyI C'CwwG_G
Cuts at: 1279 1633 1279
Size: 354 3791
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1728 1861 2320 2529 1728
Size: 133 459 209 3344
Fragments arranged by size:
3344 459 209 133
TatI w'GTAC_w
Cuts at: 505 3660 505
Size: 3155 990
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 3662 3694 246
Size: 32 3384 32 697
Fragments arranged by size:
3384 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 1176 1176
Size: 4145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1640 114
Size: 1526 2619
XhoI C'TCGA_G
Cuts at: 206 206
Size: 4145
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I BtgI ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI ZraI