Restriction map for
pF1KB4280
(Circular) (Six-base) MAPSORT of: pF1KB4280.seq Check: 8229 from: 1 to: 3926
pF1KB4280 3926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 695 942 695
Size: 247 3679
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1751 2183 2536 3198 655
Size: 1096 432 353 662 1383
Fragments arranged by size:
1383 1096 662 432 353
AflIII A'CryG_T
Cuts at: 801 1278 3078 3359 801
Size: 477 1800 281 1368
Fragments arranged by size:
1800 1368 477 281
AgeI A'CCGG_T
Cuts at: 692 692
Size: 3926
AleI CACnn'nnGTG
Cuts at: 598 598
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 1231 2669 1231
Size: 1438 2488
ApaI G_GGCC'C
Cuts at: 901 920 901
Size: 19 3907
ApaLI G'TGCA_C
Cuts at: 828 2764 828
Size: 1936 1990
ApoI r'AATT_y
Cuts at: 930 3279 3535 930
Size: 2349 256 1321
Fragments arranged by size:
2349 1321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 313 946 313
Size: 633 3293
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 695 942 1356 1604 1639 695
Size: 247 414 248 35 2982
Fragments arranged by size:
2982 414 248 247 35
BanII G_rGCy'C
Cuts at: 314 901 920 940 1970 314
Size: 587 19 20 1030 2270
Fragments arranged by size:
2270 1030 587 20 19
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbsI GAAGACnn'nnnn_
Cuts at: 663 663
Size: 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3892
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2880 3926 1824
Size: 1056 1046 1824
Fragments arranged by size:
1824 1056 1046
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglI GCCn_nnn'nGGC
Cuts at: 215 215
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 832 901 920 1551 1644 2768 832
Size: 69 19 631 93 1124 1990
Fragments arranged by size:
1990 1124 631 93 69 19
BmrI ACTGGGnnnn_n'
Cuts at: 201 1138 1219 1554 3698 201
Size: 937 81 335 2144 429
Fragments arranged by size:
2144 937 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 466 898 355
Size: 111 432 3383
Fragments arranged by size:
3383 432 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2695 2993 1102
Size: 879 714 298 2035
Fragments arranged by size:
2035 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 3926
BsaAI yAC'GTr
Cuts at: 804 1279 804
Size: 475 3451
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 410 1357 1605 2307 3803 410
Size: 947 248 702 1496 533
Fragments arranged by size:
1496 947 702 533 248
BsaWI w'CCGG_w
Cuts at: 692 1115 1636 2314 2725 2872 3146 692
Size: 423 521 678 411 147 274 1472
Fragments arranged by size:
1472 678 521 423 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 418
Size: 30 3896
BseYI C'CCAG_C
Cuts at: 147 197 450 554 629 1351 2109 2774 3182
Size: 50 253 104 75 722 758 665 408
Cuts at: 3182 147
Size: 891
Fragments arranged by size:
891 758 722 665 408 253 104 75
50
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2744 104
Size: 1364 46 1230 1286
Fragments arranged by size:
1364 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 428 832 940 1718 1908 2768 428
Size: 404 108 778 190 860 1586
Fragments arranged by size:
1586 860 778 404 190 108
Bsp1286I G_dGCh'C
Cuts at: 314 428 832 901 920 940 1551 1644 1718
Size: 114 404 69 19 20 611 93 74
Cuts at: 1718 1908 1970 2768 314
Size: 190 62 798 1472
Fragments arranged by size:
1472 798 611 404 190 114 93 74
69 62 20 19
BspHI T'CATG_A
Cuts at: 2358 2358
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 260 707 976 1190 1492 1873 260
Size: 447 269 214 302 381 2313
Fragments arranged by size:
2313 447 381 302 269 214
BsrBI CCG'CTC
Cuts at: 50 2218 2272 3756 3921 50
Size: 2168 54 1484 165 55
Fragments arranged by size:
2168 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 692 1924 2105 692
Size: 1232 181 2513
Fragments arranged by size:
2513 1232 181
BssSI C'ACGA_G
Cuts at: 2197 2905 2197
Size: 708 3218
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstEII G'GTnAC_C
Cuts at: 591 591
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 381 381
Size: 3926
BstYI r'GATC_y
Cuts at: 1 951 1418 1441 1776 2022 2328 2340 2426
Size: 950 467 23 335 246 306 12 86
Cuts at: 2426 3478 1
Size: 1052 449
Fragments arranged by size:
1052 950 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1876 2057 2220 1876
Size: 181 163 3582
Fragments arranged by size:
3582 181 163
BtsI GCAGTG_nn'
Cuts at: 1169 1169
Size: 3926
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2976 1632
Size: 1344 2582
EaeI y'GGCC_r
Cuts at: 1511 1685 2076 2103 3448 1511
Size: 174 391 27 1345 1989
Fragments arranged by size:
1989 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2876 3022 3736 2134
Size: 742 146 714 2324
Fragments arranged by size:
2324 742 714 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 466 655 898 1751 2183 2536 3198 355
Size: 111 189 243 853 432 353 662 1083
Fragments arranged by size:
1083 853 662 432 353 243 189 111
EcoNI CCTnn'n_nnAGG
Cuts at: 880 880
Size: 3926
EcoO109I rG'GnC_Cy
Cuts at: 117 201 455 897 916 1065 117
Size: 84 254 442 19 149 2978
Fragments arranged by size:
2978 442 254 149 84 19
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 481 1707 481
Size: 1226 2700
FspAI rTGC'GCAy
Cuts at: 481 481
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2838 3609 1360
Size: 248 1230 771 1677
Fragments arranged by size:
1677 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1768 1798 1800 1830 1768
Size: 30 2 30 3864
Fragments arranged by size:
3864 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 699 946 699
Size: 247 3679
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 585 2685 2869 585
Size: 2100 184 1642
Fragments arranged by size:
2100 1642 184
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 379 598 1915 2042 379
Size: 219 1317 127 2263
Fragments arranged by size:
2263 1317 219 127
MspA1I CmG'CkG
Cuts at: 139 475 558 633 818 1037 1351 1711 2495
Size: 336 83 75 185 219 314 360 784
Cuts at: 2495 2740 139
Size: 245 1325
Fragments arranged by size:
1325 784 360 336 314 245 219 185
83 75
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 307 979 1479 3082 307
Size: 672 500 1603 1151
Fragments arranged by size:
1603 1151 672 500
PciI A'CATG_T
Cuts at: 3078 3078
Size: 3926
PflMI CCAn_nnn'nTGG
Cuts at: 561 561
Size: 3926
PfoI T'CCnGG_A
Cuts at: 848 848
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PmlI CAC'GTG
Cuts at: 804 804
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2372 2404 2372
Size: 32 3894
PspOMI G'GGCC_C
Cuts at: 897 916 897
Size: 19 3907
PstI C_TGCA'G
Cuts at: 239 302 973 1658 239
Size: 63 671 685 2507
Fragments arranged by size:
2507 685 671 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 139 475 633 818 1351 1711 139
Size: 336 158 185 533 360 2354
Fragments arranged by size:
2354 533 360 336 185 158
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SfcI C'TryA_G
Cuts at: 33 235 298 969 1654 2622 2813 33
Size: 202 63 671 685 968 191 1146
Fragments arranged by size:
1146 968 685 671 202 191 63
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 315 948 315
Size: 633 3293
SmlI C'TyrA_G
Cuts at: 1081 1996 2710 2972 1081
Size: 915 714 262 2035
Fragments arranged by size:
2035 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 307 979 1479 307
Size: 672 500 2754
Fragments arranged by size:
2754 672 500
StyI C'CwwG_G
Cuts at: 269 1060 1414 269
Size: 791 354 2781
Fragments arranged by size:
2781 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 250 568 1509 1642 2101 2310 250
Size: 318 941 133 459 209 1866
Fragments arranged by size:
1866 941 459 318 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 3443 3475 418
Size: 32 2993 32 869
Fragments arranged by size:
2993 869 32 32
Tth111I GACn'n_nGTC
Cuts at: 589 667 1723 589
Size: 78 1056 2792
Fragments arranged by size:
2792 1056 78
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 313 946 313
Size: 633 3293
XmnI GAAnn'nnTTC
Cuts at: 226 226
Size: 3926
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BseRI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI