Restriction map for
pF1KB4303
(Circular) (Six-base) MAPSORT of: pF1KB4303.seq Check: 5639 from: 1 to: 5018
pF1KB4303 5018 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1322 1846 1322
Size: 524 4494
AccI GT'mk_AC
Cuts at: 2056 2056
Size: 5018
Acc65I G'GTAC_C
Cuts at: 2034 2034
Size: 5018
AclI AA'CG_TT
Cuts at: 4858 4858
Size: 5018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 481 1490 2843 3275 3628 4290 379
Size: 102 1009 1353 432 353 662 1107
Fragments arranged by size:
1353 1107 1009 662 432 353 102
AflII C'TTAA_G
Cuts at: 319 319
Size: 5018
AflIII A'CryG_T
Cuts at: 500 1416 1668 2370 4170 4451 500
Size: 916 252 702 1800 281 1067
Fragments arranged by size:
1800 1067 916 702 281 252
AhdI GACnn_n'nnGTC
Cuts at: 849 849
Size: 5018
AlwNI CAG_nnn'CTG
Cuts at: 1489 1507 1575 2323 3761 1489
Size: 18 68 748 1438 2746
Fragments arranged by size:
2746 1438 748 68 18
ApaI G_GGCC'C
Cuts at: 908 908
Size: 5018
ApaLI G'TGCA_C
Cuts at: 694 712 3856 694
Size: 18 3144 1856
Fragments arranged by size:
3144 1856 18
ApoI r'AATT_y
Cuts at: 2022 4371 4627 2022
Size: 2349 256 2413
Fragments arranged by size:
2413 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5018
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
AvaI C'yCGr_G
Cuts at: 856 907 1067 1745 2038 856
Size: 51 160 678 293 3836
Fragments arranged by size:
3836 678 293 160 51
AvrII C'CTAG_G
Cuts at: 181 1860 181
Size: 1679 3339
BamHI G'GATC_C
Cuts at: 1755 2043 1755
Size: 288 4730
BanI G'GyrC_C
Cuts at: 835 1216 1978 2034 2448 2696 2731 835
Size: 381 762 56 414 248 35 3122
Fragments arranged by size:
3122 762 414 381 248 56 35
BanII G_rGCy'C
Cuts at: 305 908 1259 1442 1535 2032 3062 305
Size: 603 351 183 93 497 1030 2261
Fragments arranged by size:
2261 1030 603 497 351 183 93
BbeI G_GCGC'C
Cuts at: 1982 2452 2700 1982
Size: 470 248 4300
Fragments arranged by size:
4300 470 248
BbvCI CC'TCA_GC
Cuts at: 1508 1508
Size: 5018
BciVI GTATCCnnnnn_n'
Cuts at: 2916 3972 5018 2916
Size: 1056 1046 2916
Fragments arranged by size:
2916 1056 1046
BclI T'GATC_A
Cuts at: 2538 2538
Size: 5018
BglI GCCn_nnn'nGGC
Cuts at: 649 649
Size: 5018
BglII A'GATC_T
Cuts at: 2533 4570 2533
Size: 2037 2981
BlpI GC'TnA_GC
Cuts at: 616 2130 616
Size: 1514 3504
Bme1580I G_kGCm'C
Cuts at: 255 698 716 838 908 1193 1803 2643 2736
Size: 443 18 122 70 285 610 840 93
Cuts at: 2736 3860 255
Size: 1124 1413
Fragments arranged by size:
1413 1124 840 610 443 285 122 93
70 18
BmgBI CAC'GTC
Cuts at: 501 1671 501
Size: 1170 3848
BmrI ACTGGGnnnn_n'
Cuts at: 1176 1733 2230 2311 2646 4790 1176
Size: 557 497 81 335 2144 1404
Fragments arranged by size:
2144 1404 557 497 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 3385 1044
Size: 2341 2677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 1411 1456 1519 1567 231
Size: 1180 45 63 48 3682
Fragments arranged by size:
3682 1180 63 48 45
Bpu10I CC'TnA_GC
Cuts at: 1508 1576 1508
Size: 68 4950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1429 2194 3073 3787 4085 775
Size: 654 765 879 714 298 1708
Fragments arranged by size:
1708 879 765 714 654 298
BsaI GGTCTCn'nnnn_
Cuts at: 985 1180 4722 985
Size: 195 3542 1281
Fragments arranged by size:
3542 1281 195
BsaAI yAC'GTr
Cuts at: 591 711 1419 2371 591
Size: 120 708 952 3238
Fragments arranged by size:
3238 952 708 120
BsaBI GATnn'nnATC
Cuts at: 6 2532 4569 6
Size: 2526 2037 455
Fragments arranged by size:
2526 2037 455
BsaHI Gr'CG_yC
Cuts at: 964 1979 2449 2697 3399 4895 964
Size: 1015 470 248 702 1496 1087
Fragments arranged by size:
1496 1087 1015 702 470 248
BsaWI w'CCGG_w
Cuts at: 1136 1203 2207 2728 3406 3817 3964 4238 1136
Size: 67 1004 521 678 411 147 274 1916
Fragments arranged by size:
1916 1004 678 521 411 274 147 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1261 1291 1321 1351 1261
Size: 30 30 30 4928
Fragments arranged by size:
4928 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 1075 1564 1567 505
Size: 570 489 3 3956
Fragments arranged by size:
3956 570 489 3
BseYI C'CCAG_C
Cuts at: 294 927 1110 1429 2443 3201 3866 4274 294
Size: 633 183 319 1014 758 665 408 1038
Fragments arranged by size:
1038 1014 758 665 633 408 319 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 523
Size: 5018
BsiEI CG_ry'CG
Cuts at: 104 644 820 1783 2560 2606 3836 104
Size: 540 176 963 777 46 1230 1286
Fragments arranged by size:
1286 1230 963 777 540 176 46
BsiHKAI G_wGCw'C
Cuts at: 698 716 1025 1535 1562 2032 2810 3000 3860
Size: 18 309 510 27 470 778 190 860
Cuts at: 3860 698
Size: 1856
Fragments arranged by size:
1856 860 778 510 470 309 190 27
18
Bsp1286I G_dGCh'C
Cuts at: 255 305 698 716 838 908 1025 1193 1259
Size: 50 393 18 122 70 117 168 66
Cuts at: 1259 1442 1535 1562 1803 2032 2643 2736 2810
Size: 183 93 27 241 229 611 93 74
Cuts at: 2810 3000 3062 3860 255
Size: 190 62 798 1413
Fragments arranged by size:
1413 798 611 393 241 229 190 183
168 122 117 93 93 74 70 66
62 50 27 18
BspEI T'CCGG_A
Cuts at: 1136 1136
Size: 5018
BspHI T'CATG_A
Cuts at: 440 3450 440
Size: 3010 2008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1322 1846 2068 2282 2584 2965 611
Size: 711 524 222 214 302 381 2664
Fragments arranged by size:
2664 711 524 381 302 222 214
BsrBI CCG'CTC
Cuts at: 50 633 1689 3310 3364 4848 5013 50
Size: 583 1056 1621 54 1484 165 55
Fragments arranged by size:
1621 1484 1056 583 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2930 2930
Size: 5018
BsrFI r'CCGG_y
Cuts at: 518 698 1956 3016 3197 518
Size: 180 1258 1060 181 2339
Fragments arranged by size:
2339 1258 1060 181 180
BssHII G'CGCG_C
Cuts at: 652 652
Size: 5018
BssSI C'ACGA_G
Cuts at: 1915 3289 3997 1915
Size: 1374 708 2936
Fragments arranged by size:
2936 1374 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2395 2395
Size: 5018
BstBI TT'CG_AA
Cuts at: 1211 3379 1211
Size: 2168 2850
BstEII G'GTnAC_C
Cuts at: 262 262
Size: 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 1383 1389 1383
Size: 6 5012
BstYI r'GATC_y
Cuts at: 1 860 1755 2043 2510 2533 2868 3114 3420
Size: 859 895 288 467 23 335 246 306
Cuts at: 3420 3432 3518 4570 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 895 859 467 449 335 306 288
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1058 1351 1058
Size: 293 4725
BtgI C'CryG_G
Cuts at: 107 983 1265 1382 1751 1783 107
Size: 876 282 117 369 32 3342
Fragments arranged by size:
3342 876 369 282 117 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2968 3149 3312 2968
Size: 181 163 4674
Fragments arranged by size:
4674 181 163
BtsI GCAGTG_nn'
Cuts at: 610 1259 2261 610
Size: 649 1002 3367
Fragments arranged by size:
3367 1002 649
DraI TTT'AAA
Cuts at: 2017 2017
Size: 5018
DrdI GACnn_nn'nnGTC
Cuts at: 1051 1363 2724 4068 1051
Size: 312 1361 1344 2001
Fragments arranged by size:
2001 1361 1344 312
EaeI y'GGCC_r
Cuts at: 641 680 756 770 1780 1958 2603 2777 3168
Size: 39 76 14 1010 178 645 174 391
Cuts at: 3168 3195 4540 641
Size: 27 1345 1119
Fragments arranged by size:
1345 1119 1010 645 391 178 174 76
39 27 14
EagI C'GGCC_G
Cuts at: 641 1780 2603 641
Size: 1139 823 3056
Fragments arranged by size:
3056 1139 823
EarI CTCTTCn'nnn_
Cuts at: 457 3041 3251 457
Size: 2584 210 2224
Fragments arranged by size:
2584 2224 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 682 754 1003 3226 3968 4114 4828 682
Size: 72 249 2223 742 146 714 872
Fragments arranged by size:
2223 872 742 714 249 146 72
EcoICRI GAG'CTC
Cuts at: 1533 2030 1533
Size: 497 4521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 379 481 1411 1456 1490 1519 1567 2843
Size: 148 102 930 45 34 29 48 1276
Cuts at: 2843 3275 3628 4290 231
Size: 432 353 662 959
Fragments arranged by size:
1276 959 930 662 432 353 148 102
48 45 34 29
EcoNI CCTnn'n_nnAGG
Cuts at: 890 1549 1875 890
Size: 659 326 4033
Fragments arranged by size:
4033 659 326
EcoO109I rG'GnC_Cy
Cuts at: 600 1053 1365 1372 1656 1734 1818 1856 2157
Size: 453 312 7 284 78 84 38 301
Cuts at: 2157 600
Size: 3461
Fragments arranged by size:
3461 453 312 301 284 84 78 38
7
EcoRI G'AATT_C
Cuts at: 2022 2022
Size: 5018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 5018
FspI TGC'GCA
Cuts at: 2799 2799
Size: 5018
HaeII r_GCGC'y
Cuts at: 928 1429 1884 1982 2452 2700 3930 4701 928
Size: 501 455 98 470 248 1230 771 1245
Fragments arranged by size:
1245 1230 771 501 470 455 248 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 835 867 1261 1293 2860 2890 2892
Size: 32 37 32 394 32 1567 30 2
Cuts at: 2892 2922 766
Size: 30 2862
Fragments arranged by size:
2862 1567 394 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1627 2057 1627
Size: 430 4588
KasI G'GCGC_C
Cuts at: 1978 2448 2696 1978
Size: 470 248 4300
Fragments arranged by size:
4300 470 248
KpnI G_GTAC'C
Cuts at: 2038 2038
Size: 5018
MluI A'CGCG_T
Cuts at: 4451 4451
Size: 5018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 354 1259 3777 3961 354
Size: 905 2518 184 1411
Fragments arranged by size:
2518 1411 905 184
MscI TGG'CCA
Cuts at: 682 758 2779 682
Size: 76 2021 2921
Fragments arranged by size:
2921 2021 76
MslI CAynn'nnrTG
Cuts at: 310 1264 1381 3007 3134 310
Size: 954 117 1626 127 2194
Fragments arranged by size:
2194 1626 954 127 117
MspA1I CmG'CkG
Cuts at: 298 537 752 1038 1083 1404 1468 1486 1723
Size: 239 215 286 45 321 64 18 237
Cuts at: 1723 1805 2129 2443 2803 3587 3832 298
Size: 82 324 314 360 784 245 1484
Fragments arranged by size:
1484 784 360 324 321 314 286 245
239 237 215 82 64 45 18
NaeI GCC'GGC
Cuts at: 1958 3199 1958
Size: 1241 3777
NarI GG'CG_CC
Cuts at: 1979 2449 2697 1979
Size: 470 248 4300
Fragments arranged by size:
4300 470 248
NcoI C'CATG_G
Cuts at: 107 1265 1382 107
Size: 1158 117 3743
Fragments arranged by size:
3743 1158 117
NdeI CA'TA_TG
Cuts at: 1769 1769
Size: 5018
NgoMIV G'CCGG_C
Cuts at: 1956 3197 1956
Size: 1241 3777
NspI r_CATG'y
Cuts at: 459 1232 2071 2571 4174 459
Size: 773 839 500 1603 1303
Fragments arranged by size:
1603 1303 839 773 500
PasI CC'CwG_GG
Cuts at: 164 1928 164
Size: 1764 3254
PciI A'CATG_T
Cuts at: 4170 4170
Size: 5018
PfoI T'CCnGG_A
Cuts at: 551 1178 1535 551
Size: 627 357 4034
Fragments arranged by size:
4034 627 357
PmeI GTTT'AAAC
Cuts at: 2017 2017
Size: 5018
PmlI CAC'GTG
Cuts at: 1419 1419
Size: 5018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4986
PpuMI rG'GwC_Cy
Cuts at: 600 1053 1365 1656 1734 1856 600
Size: 453 312 291 78 122 3762
Fragments arranged by size:
3762 453 312 291 122 78
PspOMI G'GGCC_C
Cuts at: 904 904
Size: 5018
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 553 585 553
Size: 32 4986
PstI C_TGCA'G
Cuts at: 338 869 1706 2065 2750 338
Size: 531 837 359 685 2606
Fragments arranged by size:
2606 837 685 531 359
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5018
PvuII CAG'CTG
Cuts at: 537 1404 1468 1723 2443 2803 537
Size: 867 64 255 720 360 2752
Fragments arranged by size:
2752 867 720 360 255 64
RsrII CG'GwC_CG
Cuts at: 3213 3213
Size: 5018
SacI G_AGCT'C
Cuts at: 1535 2032 1535
Size: 497 4521
SalI G'TCGA_C
Cuts at: 2055 2055
Size: 5018
SanDI GG'GwC_CC
Cuts at: 1053 1734 1856 1053
Size: 681 122 4215
Fragments arranged by size:
4215 681 122
SapI GCTCTTCn'nnn_
Cuts at: 3041 3251 3041
Size: 210 4808
SbfI CC_TGCA'GG
Cuts at: 869 2065 869
Size: 1196 3822
ScaI AGT'ACT
Cuts at: 4535 4535
Size: 5018
SexAI A'CCwGG_T
Cuts at: 572 572
Size: 5018
SfcI C'TryA_G
Cuts at: 33 334 865 1702 2061 2746 3714 3905 33
Size: 301 531 837 359 685 968 191 1146
Fragments arranged by size:
1146 968 837 685 531 359 301 191
SfoI GGC'GCC
Cuts at: 1980 2450 2698 1980
Size: 470 248 4300
Fragments arranged by size:
4300 470 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
SmaI CCC'GGG
Cuts at: 1747 2040 1747
Size: 293 4725
SmlI C'TyrA_G
Cuts at: 319 790 1067 1408 2173 3088 3802 4064 319
Size: 471 277 341 765 915 714 262 1273
Fragments arranged by size:
1273 915 765 714 471 341 277 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5018
SphI G_CATG'C
Cuts at: 2071 2571 2071
Size: 500 4518
StuI AGG'CCT
Cuts at: 1865 1865
Size: 5018
StyI C'CwwG_G
Cuts at: 107 181 1265 1382 1860 2152 2506 107
Size: 74 1084 117 478 292 354 2619
Fragments arranged by size:
2619 1084 478 354 292 117 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 919 1395 2601 2734 3193 3402 919
Size: 476 1206 133 459 209 2535
Fragments arranged by size:
2535 1206 476 459 209 133
TatI w'GTAC_w
Cuts at: 4533 4533
Size: 5018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 4535 4567 508
Size: 32 3995 32 959
Fragments arranged by size:
3995 959 32 32
Tth111I GACn'n_nGTC
Cuts at: 1275 2815 1275
Size: 1540 3478
XbaI T'CTAG_A
Cuts at: 2049 2049
Size: 5018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2513 2513
Size: 5018
XhoI C'TCGA_G
Cuts at: 1067 1067
Size: 5018
XmaI C'CCGG_G
Cuts at: 1745 2038 1745
Size: 293 4725
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AleI AlfI AloI AscI BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI