Restriction map for
pF1KB4307
(Circular) (Six-base) MAPSORT of: pF1KB4307.seq Check: 9659 from: 1 to: 4115
pF1KB4307 4115 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 647 647
Size: 4115
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2372 2725 3387 1940
Size: 432 353 662 2668
Fragments arranged by size:
2668 662 432 353
AflIII A'CryG_T
Cuts at: 1467 3267 3548 1467
Size: 1800 281 2034
Fragments arranged by size:
2034 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1420 2858 1420
Size: 1438 2677
ApaLI G'TGCA_C
Cuts at: 2953 2953
Size: 4115
ApoI r'AATT_y
Cuts at: 149 520 999 1119 3468 3724 149
Size: 371 479 120 2349 256 540
Fragments arranged by size:
2349 540 479 371 256 120
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 1135 1135
Size: 4115
BamHI G'GATC_C
Cuts at: 356 1140 356
Size: 784 3331
BanI G'GyrC_C
Cuts at: 1131 1545 1793 1828 1131
Size: 414 248 35 3418
Fragments arranged by size:
3418 414 248 35
BanII G_rGCy'C
Cuts at: 500 794 1129 2159 500
Size: 294 335 1030 2456
Fragments arranged by size:
2456 1030 335 294
BbeI G_GCGC'C
Cuts at: 1549 1797 1549
Size: 248 3867
BciVI GTATCCnnnnn_n'
Cuts at: 498 799 2013 3069 4115 498
Size: 301 1214 1056 1046 498
Fragments arranged by size:
1214 1056 1046 498 301
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglI GCCn_nnn'nGGC
Cuts at: 333 333
Size: 4115
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 329 1740 1833 2957 329
Size: 1411 93 1124 1487
Fragments arranged by size:
1487 1411 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 292 1327 1408 1743 3887 292
Size: 1035 81 335 2144 520
Fragments arranged by size:
2144 1035 520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2170 2884 3182 1291
Size: 879 714 298 2224
Fragments arranged by size:
2224 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3819 3819
Size: 4115
BsaAI yAC'GTr
Cuts at: 324 1468 324
Size: 1144 2971
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 644 1546 1794 2496 3992 644
Size: 902 248 702 1496 767
Fragments arranged by size:
1496 902 767 702 248
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2914 3061 3335 1304
Size: 521 678 411 147 274 2084
Fragments arranged by size:
2084 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 937 937
Size: 4115
BseYI C'CCAG_C
Cuts at: 328 942 1540 2298 2963 3371 328
Size: 614 598 758 665 408 1072
Fragments arranged by size:
1072 758 665 614 598 408
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2933 104
Size: 1553 46 1230 1286
Fragments arranged by size:
1553 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 500 794 1129 1907 2097 2957 500
Size: 294 335 778 190 860 1658
Fragments arranged by size:
1658 860 778 335 294 190
BsiWI C'GTAC_G
Cuts at: 296 296
Size: 4115
BsmBI CGTCTCn'nnnn_
Cuts at: 635 1100 635
Size: 465 3650
Bsp1286I G_dGCh'C
Cuts at: 329 500 794 1129 1740 1833 1907 2097 2159
Size: 171 294 335 611 93 74 190 62
Cuts at: 2159 2957 329
Size: 798 1487
Fragments arranged by size:
1487 798 611 335 294 190 171 93
74 62
BspHI T'CATG_A
Cuts at: 2547 2547
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 1165 1379 1681 2062 739
Size: 426 214 302 381 2792
Fragments arranged by size:
2792 426 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 2113 2294 2113
Size: 181 3934
BssSI C'ACGA_G
Cuts at: 2386 3094 2386
Size: 708 3407
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstYI r'GATC_y
Cuts at: 1 356 485 1140 1607 1630 1965 2211 2517
Size: 355 129 655 467 23 335 246 306
Cuts at: 2517 2529 2615 3667 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 655 467 449 355 335 306 246
129 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 914 2065 2246 2409 914
Size: 1151 181 163 2620
Fragments arranged by size:
2620 1151 181 163
BtsI GCAGTG_nn'
Cuts at: 353 1358 353
Size: 1005 3110
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 3165 1821
Size: 1344 2771
EaeI y'GGCC_r
Cuts at: 857 1700 1874 2265 2292 3637 857
Size: 843 174 391 27 1345 1335
Fragments arranged by size:
1345 1335 843 391 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 3065 3211 3925 2323
Size: 742 146 714 2513
Fragments arranged by size:
2513 742 714 146
EcoICRI GAG'CTC
Cuts at: 498 792 1127 498
Size: 294 335 3486
Fragments arranged by size:
3486 335 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1940 2372 2725 3387 286
Size: 1654 432 353 662 1014
Fragments arranged by size:
1654 1014 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1254 1254
Size: 4115
EcoRI G'AATT_C
Cuts at: 999 1119 999
Size: 120 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 1549 1797 3027 3798 1549
Size: 248 1230 771 1866
Fragments arranged by size:
1866 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 913 940 945 964 972 996 1066
Size: 32 688 27 5 19 8 24 70
Cuts at: 1066 1098 1957 1987 1989 2019 193
Size: 32 859 30 2 30 2289
Fragments arranged by size:
2289 859 688 70 32 32 30 30
27 24 19 8 5 2
HincII GTy'rAC
Cuts at: 972 1154 972
Size: 182 3933
KasI G'GCGC_C
Cuts at: 1545 1793 1545
Size: 248 3867
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MfeI C'AATT_G
Cuts at: 740 740
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2874 3058 2874
Size: 184 3931
MscI TGG'CCA
Cuts at: 859 1876 859
Size: 1017 3098
MslI CAynn'nnrTG
Cuts at: 346 844 2104 2231 346
Size: 498 1260 127 2230
Fragments arranged by size:
2230 1260 498 127
MspA1I CmG'CkG
Cuts at: 332 1226 1540 1900 2684 2929 332
Size: 894 314 360 784 245 1518
Fragments arranged by size:
1518 894 784 360 314 245
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 4115
NarI GG'CG_CC
Cuts at: 1546 1794 1546
Size: 248 3867
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 4115
NspI r_CATG'y
Cuts at: 1168 1668 3271 1168
Size: 500 1603 2012
Fragments arranged by size:
2012 1603 500
PciI A'CATG_T
Cuts at: 3267 3267
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PmlI CAC'GTG
Cuts at: 324 324
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2561 2593 2561
Size: 32 4083
PstI C_TGCA'G
Cuts at: 963 1162 1847 963
Size: 199 685 3231
Fragments arranged by size:
3231 685 199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 1540 1900 1540
Size: 360 3755
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 500 794 1129 500
Size: 294 335 3486
Fragments arranged by size:
3486 335 294
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 3632 3632
Size: 4115
SfcI C'TryA_G
Cuts at: 33 959 1158 1843 2811 3002 33
Size: 926 199 685 968 191 1146
Fragments arranged by size:
1146 968 926 685 199 191
SfoI GGC'GCC
Cuts at: 1547 1795 1547
Size: 248 3867
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 1270 2185 2899 3161 1270
Size: 915 714 262 2224
Fragments arranged by size:
2224 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StyI C'CwwG_G
Cuts at: 212 1249 1603 212
Size: 1037 354 2724
Fragments arranged by size:
2724 1037 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 667 1698 1831 2290 2499 667
Size: 1031 133 459 209 2283
Fragments arranged by size:
2283 1031 459 209 133
TatI w'GTAC_w
Cuts at: 660 3630 660
Size: 2970 1145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 797 829 1061 1093 3632 3664 797
Size: 32 232 32 2539 32 1248
Fragments arranged by size:
2539 1248 232 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 1610 423
Size: 1187 2928
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
ZraI GAC'GTC
Cuts at: 645 645
Size: 4115
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsgI BsmI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI